ES2911421T3 - Adaptadores, métodos y composiciones mejorados para secuenciación dúplex - Google Patents

Adaptadores, métodos y composiciones mejorados para secuenciación dúplex Download PDF

Info

Publication number
ES2911421T3
ES2911421T3 ES16822832T ES16822832T ES2911421T3 ES 2911421 T3 ES2911421 T3 ES 2911421T3 ES 16822832 T ES16822832 T ES 16822832T ES 16822832 T ES16822832 T ES 16822832T ES 2911421 T3 ES2911421 T3 ES 2911421T3
Authority
ES
Spain
Prior art keywords
nucleic acid
adapter
strand
sequence
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES16822832T
Other languages
English (en)
Spanish (es)
Inventor
Lawrence A Loeb
Michael W Schmitt
Jesse J Salk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Twinstrand Biosciences Inc
Original Assignee
Twinstrand Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Twinstrand Biosciences Inc filed Critical Twinstrand Biosciences Inc
Application granted granted Critical
Publication of ES2911421T3 publication Critical patent/ES2911421T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES16822832T 2015-12-08 2016-12-08 Adaptadores, métodos y composiciones mejorados para secuenciación dúplex Active ES2911421T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562264822P 2015-12-08 2015-12-08
US201662281917P 2016-01-22 2016-01-22
PCT/US2016/065605 WO2017100441A1 (en) 2015-12-08 2016-12-08 Improved adapters, methods, and compositions for duplex sequencing

Publications (1)

Publication Number Publication Date
ES2911421T3 true ES2911421T3 (es) 2022-05-19

Family

ID=57750605

Family Applications (1)

Application Number Title Priority Date Filing Date
ES16822832T Active ES2911421T3 (es) 2015-12-08 2016-12-08 Adaptadores, métodos y composiciones mejorados para secuenciación dúplex

Country Status (11)

Country Link
US (3) US11332784B2 (enExample)
EP (2) EP3387152B1 (enExample)
JP (3) JP6975507B2 (enExample)
CN (1) CN109072294A (enExample)
AU (1) AU2016366231B2 (enExample)
CA (1) CA3006792A1 (enExample)
ES (1) ES2911421T3 (enExample)
IL (1) IL259788B2 (enExample)
PL (1) PL3387152T3 (enExample)
PT (1) PT3387152T (enExample)
WO (1) WO2017100441A1 (enExample)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
EP2814959B1 (en) 2012-02-17 2018-01-17 Fred Hutchinson Cancer Research Center Compositions and methods for accurately identifying mutations
HUE051845T2 (hu) 2012-03-20 2021-03-29 Univ Washington Through Its Center For Commercialization Módszerek a tömegesen párhuzamos DNS-szekvenálás hibaarányának csökkentésére duplex konszenzus szekvenálással
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
DE202013012824U1 (de) 2012-09-04 2020-03-10 Guardant Health, Inc. Systeme zum Erfassen von seltenen Mutationen und einer Kopienzahlvariation
JP6656733B2 (ja) 2013-08-05 2020-03-04 ツイスト バイオサイエンス コーポレーション 新規合成した遺伝子ライブラリ
SG10201804519RA (en) 2013-12-28 2018-07-30 Guardant Health Inc Methods and systems for detecting genetic variants
US10954559B2 (en) * 2014-11-21 2021-03-23 Mgi Tech Co., Ltd. Bubble-shaped adaptor element and method of constructing sequencing library with bubble-shaped adaptor element
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
US10844373B2 (en) 2015-09-18 2020-11-24 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
EP3384077A4 (en) 2015-12-01 2019-05-08 Twist Bioscience Corporation FUNCTIONALIZED SURFACES AND MANUFACTURE THEREOF
CA3006792A1 (en) 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
JP2019507585A (ja) 2015-12-17 2019-03-22 ガーダント ヘルス， インコーポレイテッド 無細胞ｄｎａの分析による腫瘍遺伝子コピー数を決定するための方法
ES3002715T3 (en) 2016-04-14 2025-03-07 Guardant Health Inc Methods for early detection of cancer
US11384382B2 (en) 2016-04-14 2022-07-12 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
EP4180539A1 (en) 2016-07-12 2023-05-17 Qiagen Sciences, LLC Single end duplex dna sequencing
WO2018015318A1 (en) 2016-07-18 2018-01-25 F. Hoffmann-La Roche Ag Method for generating single-stranded circular dna libraries for single molecule sequencing
WO2018031588A1 (en) * 2016-08-09 2018-02-15 Takara Bio Usa, Inc. Nucleic acid adaptors with molecular identification sequences and use thereof
CN109996876A (zh) 2016-08-22 2019-07-09 特韦斯特生物科学公司 从头合成的核酸文库
KR102217487B1 (ko) 2016-09-21 2021-02-23 트위스트 바이오사이언스 코포레이션 핵산 기반 데이터 저장
JP7169975B2 (ja) 2016-12-16 2022-11-11 ツイスト バイオサイエンス コーポレーション 免疫シナプスの変異体ライブラリーおよびその合成
WO2018125892A1 (en) 2016-12-28 2018-07-05 Quest Diagnostics Investments Llc Compositions and methods for detecting circulating tumor dna
KR102701404B1 (ko) 2017-01-18 2024-08-30 일루미나, 인코포레이티드 이종 분자 길이를 가진 고유 분자 인덱스 세트의 생성 및 오류 수정 방법 및 시스템
EP4556433A3 (en) 2017-02-22 2025-08-06 Twist Bioscience Corporation Nucleic acid based data storage
US10894959B2 (en) 2017-03-15 2021-01-19 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CN110520542B (zh) 2017-03-23 2024-06-14 华盛顿大学 用于靶向核酸序列富集的方法及在错误纠正的核酸测序中的应用
WO2018183942A1 (en) * 2017-03-31 2018-10-04 Grail, Inc. Improved library preparation and use thereof for sequencing-based error correction and/or variant identification
CN119824552A (zh) 2017-05-01 2025-04-15 伊鲁米那股份有限公司 用于多重大规模平行测序的最佳索引序列
AU2018266377B2 (en) 2017-05-08 2024-06-20 Illumina, Inc. Universal short adapters for indexing of polynucleotide samples
KR102784398B1 (ko) 2017-06-12 2025-03-19 트위스트 바이오사이언스 코포레이션 심리스 핵산 어셈블리를 위한 방법
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
GB2583590A (en) 2017-10-20 2020-11-04 Twist Bioscience Corp Heated nanowells for polynucleotide synthesis
FI3707723T3 (fi) 2017-11-06 2023-12-13 Illumina Inc Nukleiinihappojen indeksointitekniikoita
SG11202003885UA (en) 2017-11-08 2020-05-28 Twinstrand Biosciences Inc Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
KR20250069684A (ko) 2018-01-04 2025-05-19 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
CN108300716B (zh) * 2018-01-05 2020-06-30 武汉康测科技有限公司 接头元件、其应用和基于不对称多重pcr进行靶向测序文库构建的方法
JP2021515579A (ja) 2018-03-15 2021-06-24 ツインストランド・バイオサイエンシズ・インコーポレイテッドＴｗｉｎｓｔｒａｎｄ Ｂｉｏｓｃｉｅｎｃｅｓ， Ｉｎｃ． 配列決定用途および他の核酸物質インテロゲーションのための核酸物質を濃縮するための方法および試薬
GB201804641D0 (en) 2018-03-22 2018-05-09 Inivata Ltd Methods of sequencing nucleic acids and error correction of sequence reads
WO2019183640A1 (en) * 2018-03-23 2019-09-26 Board Of Regents, The University Of Texas System Efficient sequencing of dsdna with extremely low level of errors
BR112020023296A2 (pt) * 2018-05-16 2021-05-04 Twinstrand Biosciences, Inc. métodos e reagentes para resolver misturas de ácido nucleico e populações de células mistas e aplicações associadas
JP2021526366A (ja) 2018-05-18 2021-10-07 ツイスト バイオサイエンス コーポレーション 核酸ハイブリダイゼーションのためのポリヌクレオチド、試薬、および方法
BR112021000409A2 (pt) * 2018-07-12 2021-04-06 Twinstrand Biosciences, Inc. Métodos e reagentes para caracterizar edição genômica, expansão clonal e aplicações associadas
US12297490B2 (en) 2018-08-28 2025-05-13 Sophia Genetics S.A. Methods for asymmetric DNA library generation and optionally integrated duplex sequencing
EP3853362A1 (en) 2018-09-21 2021-07-28 F. Hoffmann-La Roche AG System and method for modular and combinatorial nucleic acid sample preparation for sequencing
CN113439125A (zh) 2018-10-16 2021-09-24 特温斯特兰德生物科学有限公司 用于通过汇集对大量样品进行高效基因分型的方法和试剂
CN111378557B (zh) 2018-12-26 2023-06-06 财团法人工业技术研究院 用于产生液珠的管状结构及液珠产生方法
EP3902816A4 (en) 2018-12-26 2022-09-14 Twist Bioscience Corporation HIGHLY ACCURATE POLYNUCLEOTIDE DE NOVO SYNTHESIS
AU2020227672A1 (en) * 2019-02-25 2021-10-07 Twist Bioscience Corporation Compositions and methods for next generation sequencing
CN113785057A (zh) 2019-02-26 2021-12-10 特韦斯特生物科学公司 用于抗体优化的变异核酸文库
EP3930753A4 (en) 2019-02-26 2023-03-29 Twist Bioscience Corporation VARIANT NUCLEIC ACID LIBRARIES FOR GLP1 RECEPTOR
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US20220220543A1 (en) * 2019-08-01 2022-07-14 Twinstrand Biosciences, Inc. Methods and reagents for nucleic acid sequencing and associated applications
US12173282B2 (en) 2019-09-23 2024-12-24 Twist Bioscience, Inc. Antibodies that bind CD3 epsilon
EP4464827A3 (en) 2019-09-23 2025-02-26 Twist Bioscience Corporation Variant nucleic acid libraries for crth2
CN112176419B (zh) * 2019-10-16 2022-03-22 中国医学科学院肿瘤医院 一种检测ctDNA中肿瘤特异基因的变异和甲基化的方法
BR112022008752A2 (pt) 2019-11-06 2022-08-16 Univ Leland Stanford Junior Métodos e sistemas para a análise de moléculas de ácido nucleico
US11783912B2 (en) 2021-05-05 2023-10-10 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for analyzing nucleic acid molecules
WO2024015869A2 (en) * 2022-07-12 2024-01-18 University Of Washington Systems and methods for variant detection in cells
AU2023316513A1 (en) * 2022-07-25 2025-02-13 Mgi Tech Co., Ltd. Methods of in-solution positional co-barcoding for sequencing long dna molecules

Family Cites Families (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5308751A (en) 1992-03-23 1994-05-03 General Atomics Method for sequencing double-stranded DNA
ES2563643T3 (es) * 1997-04-01 2016-03-15 Illumina Cambridge Limited Método de secuenciación de ácido nucleico
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
EP1124990B1 (en) 1998-10-27 2006-01-18 Affymetrix, Inc. Complexity management and analysis of genomic dna
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
US7300751B2 (en) 2000-06-30 2007-11-27 Syngenta Participations Ag Method for identification of genetic markers
US6632611B2 (en) 2001-07-20 2003-10-14 Affymetrix, Inc. Method of target enrichment and amplification
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
US20040002073A1 (en) 2001-10-15 2004-01-01 Li Alice Xiang Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection
US7406385B2 (en) 2001-10-25 2008-07-29 Applera Corporation System and method for consensus-calling with per-base quality values for sample assemblies
US7459273B2 (en) 2002-10-04 2008-12-02 Affymetrix, Inc. Methods for genotyping selected polymorphism
US7452699B2 (en) 2003-01-15 2008-11-18 Dana-Farber Cancer Institute, Inc. Amplification of DNA in a hairpin structure, and applications
EP1606417A2 (en) 2003-03-07 2005-12-21 Rubicon Genomics Inc. In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna
EP1709203A2 (en) 2004-01-23 2006-10-11 Lingvitae AS Improving polynucleotide ligation reactions
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US8796441B2 (en) 2005-04-13 2014-08-05 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Human sweet and umami taste receptor variants
EP2302070B1 (en) 2005-06-23 2012-08-22 Keygene N.V. Strategies for high throughput identification and detection of polymorphisms
CA2623539C (en) 2005-09-29 2015-12-15 Keygene N.V. High throughput screening of mutagenized populations
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US7537897B2 (en) 2006-01-23 2009-05-26 Population Genetics Technologies, Ltd. Molecular counting
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
DK2002017T3 (en) 2006-04-04 2015-09-07 Keygene Nv High-capacity detection of molecular markers based on restriction fragments
WO2008093098A2 (en) * 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
EP2201143B2 (en) 2007-09-21 2016-08-24 Katholieke Universiteit Leuven Tools and methods for genetic tests using next generation sequencing
CN102084001B (zh) 2008-03-28 2015-03-18 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序的组合物和方法
US8029993B2 (en) * 2008-04-30 2011-10-04 Population Genetics Technologies Ltd. Asymmetric adapter library construction
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
WO2010056728A1 (en) 2008-11-11 2010-05-20 Helicos Biosciences Corporation Nucleic acid encoding for multiplex analysis
CN102369298B (zh) 2009-01-30 2017-03-22 牛津纳米孔技术有限公司 跨膜测序中用于核酸构建体的衔接体
US20100331204A1 (en) 2009-02-13 2010-12-30 Jeff Jeddeloh Methods and systems for enrichment of target genomic sequences
US20120165202A1 (en) 2009-04-30 2012-06-28 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
EP2248914A1 (en) 2009-05-05 2010-11-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. The use of class IIB restriction endonucleases in 2nd generation sequencing applications
CN102482668A (zh) 2009-08-20 2012-05-30 群体遗传学科技有限公司 分子内核酸重排的组合物和方法
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9080210B2 (en) 2010-06-09 2015-07-14 Keygene N.V. High throughput screening using combinatorial sequence barcodes
US20120244525A1 (en) 2010-07-19 2012-09-27 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
US20120238738A1 (en) 2010-07-19 2012-09-20 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
ES2523140T3 (es) 2010-09-21 2014-11-21 Population Genetics Technologies Ltd. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
WO2012042374A2 (en) 2010-10-01 2012-04-05 Anssi Jussi Nikolai Taipale Method of determining number or concentration of molecules
US9074251B2 (en) * 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2635679B1 (en) 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
DK2652155T3 (en) 2010-12-16 2017-02-13 Gigagen Inc Methods for Massive Parallel Analysis of Nucleic Acids in Single Cells
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
WO2012142213A2 (en) 2011-04-15 2012-10-18 The Johns Hopkins University Safe sequencing system
AU2012281242B2 (en) 2011-07-08 2016-12-22 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
EP2753712B1 (en) 2011-09-06 2017-03-22 Gen-Probe Incorporated Closed nucleic acid structures
EP2753715A4 (en) * 2011-09-09 2015-05-20 Univ Leland Stanford Junior METHOD FOR OBTAINING A SEQUENCE
JP5418579B2 (ja) 2011-12-06 2014-02-19 株式会社デンソー 開閉眼検出装置
US9650628B2 (en) 2012-01-26 2017-05-16 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library regeneration
US20160153039A1 (en) 2012-01-26 2016-06-02 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US9528107B2 (en) * 2012-01-31 2016-12-27 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
EP2814959B1 (en) 2012-02-17 2018-01-17 Fred Hutchinson Cancer Research Center Compositions and methods for accurately identifying mutations
ES2776673T3 (es) 2012-02-27 2020-07-31 Univ North Carolina Chapel Hill Métodos y usos para etiquetas moleculares
US9670529B2 (en) 2012-02-28 2017-06-06 Population Genetics Technologies Ltd. Method for attaching a counter sequence to a nucleic acid sample
NO2694769T3 (enExample) * 2012-03-06 2018-03-03
US9862995B2 (en) 2012-03-13 2018-01-09 Abhijit Ajit Patel Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
HUE051845T2 (hu) 2012-03-20 2021-03-29 Univ Washington Through Its Center For Commercialization Módszerek a tömegesen párhuzamos DNS-szekvenálás hibaarányának csökkentésére duplex konszenzus szekvenálással
CA2873176C (en) 2012-05-10 2024-02-27 The General Hospital Corporation Methods for determining a nucleotide sequence
CA2872141C (en) 2012-05-31 2016-01-19 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
DE202013012824U1 (de) 2012-09-04 2020-03-10 Guardant Health, Inc. Systeme zum Erfassen von seltenen Mutationen und einer Kopienzahlvariation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
CN102877136B (zh) * 2012-09-24 2014-03-12 上海交通大学 基于基因组简化与二代测序dna文库构建方法及试剂盒
AU2013382098B2 (en) * 2013-03-13 2019-02-07 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US9290800B2 (en) * 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
US10087481B2 (en) * 2013-03-19 2018-10-02 New England Biolabs, Inc. Enrichment of target sequences
US9873907B2 (en) 2013-05-29 2018-01-23 Agilent Technologies, Inc. Method for fragmenting genomic DNA using CAS9
EP3049523B1 (en) * 2013-09-24 2019-08-07 Somalogic, Inc. Multiaptamer target detection
SG10201804519RA (en) 2013-12-28 2018-07-30 Guardant Health Inc Methods and systems for detecting genetic variants
EP3363904B1 (en) 2014-01-31 2019-10-23 Swift Biosciences, Inc. Improved methods for processing dna substrates
ES2925014T3 (es) 2014-09-12 2022-10-13 Univ Leland Stanford Junior Identificación y uso de ácidos nucleicos circulantes
CA3012122A1 (en) 2015-01-23 2016-07-28 Washington University Detection of rare sequence variants, methods and compositions therefor
US10686637B2 (en) 2015-02-20 2020-06-16 Nec Corporation Receiving apparatus and receiving method
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
CN107849600A (zh) 2015-06-09 2018-03-27 生命技术公司 用于分子标记的方法、系统、组合物、试剂盒、装置和计算机可读媒体
US10465241B2 (en) 2015-06-15 2019-11-05 The Board Of Trustees Of The Leleand Stanford Junior University High resolution STR analysis using next generation sequencing
GB201515557D0 (en) 2015-09-02 2015-10-14 14M Genomics Ltd Method of sequencing
CN108350491A (zh) 2015-11-18 2018-07-31 加利福尼亚太平洋生物科学股份有限公司 将核酸加载到基材上
CA3006792A1 (en) 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
AU2017209330B2 (en) 2016-01-22 2023-05-04 Grail, Llc Variant based disease diagnostics and tracking
WO2017127741A1 (en) 2016-01-22 2017-07-27 Grail, Inc. Methods and systems for high fidelity sequencing
EP4180539A1 (en) 2016-07-12 2023-05-17 Qiagen Sciences, LLC Single end duplex dna sequencing
EP3800461A1 (en) 2016-07-29 2021-04-07 Sysmex Corporation Smear analysis system and method
WO2018031588A1 (en) 2016-08-09 2018-02-15 Takara Bio Usa, Inc. Nucleic acid adaptors with molecular identification sequences and use thereof
WO2018057928A1 (en) 2016-09-23 2018-03-29 Grail, Inc. Methods of preparing and analyzing cell-free nucleic acid sequencing libraries
US10982351B2 (en) 2016-12-23 2021-04-20 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
CN110520542B (zh) 2017-03-23 2024-06-14 华盛顿大学 用于靶向核酸序列富集的方法及在错误纠正的核酸测序中的应用
WO2018183942A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Improved library preparation and use thereof for sequencing-based error correction and/or variant identification
SG11202003885UA (en) 2017-11-08 2020-05-28 Twinstrand Biosciences Inc Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
SG11202007648WA (en) 2018-02-13 2020-09-29 Twinstrand Biosciences Inc Methods and reagents for detecting and assessing genotoxicity
JP2021515579A (ja) 2018-03-15 2021-06-24 ツインストランド・バイオサイエンシズ・インコーポレイテッドＴｗｉｎｓｔｒａｎｄ Ｂｉｏｓｃｉｅｎｃｅｓ， Ｉｎｃ． 配列決定用途および他の核酸物質インテロゲーションのための核酸物質を濃縮するための方法および試薬
WO2019183640A1 (en) 2018-03-23 2019-09-26 Board Of Regents, The University Of Texas System Efficient sequencing of dsdna with extremely low level of errors
BR112020023296A2 (pt) 2018-05-16 2021-05-04 Twinstrand Biosciences, Inc. métodos e reagentes para resolver misturas de ácido nucleico e populações de células mistas e aplicações associadas
BR112021000409A2 (pt) 2018-07-12 2021-04-06 Twinstrand Biosciences, Inc. Métodos e reagentes para caracterizar edição genômica, expansão clonal e aplicações associadas
KR102662200B1 (ko) 2019-07-15 2024-04-29 엘지디스플레이 주식회사 유기발광표시장치

Also Published As

Publication number Publication date
EP3387152A1 (en) 2018-10-17
JP2019509015A (ja) 2019-04-04
JP7548579B2 (ja) 2024-09-10
JP6975507B2 (ja) 2021-12-01
IL259788B1 (en) 2023-01-01
CA3006792A1 (en) 2017-06-15
PT3387152T (pt) 2022-04-19
PL3387152T3 (pl) 2022-05-09
US20170211140A1 (en) 2017-07-27
CN109072294A (zh) 2018-12-21
US20220267841A1 (en) 2022-08-25
JP2022017453A (ja) 2022-01-25
IL259788B2 (en) 2023-05-01
EP4043584A1 (en) 2022-08-17
AU2016366231B2 (en) 2022-12-15
US11332784B2 (en) 2022-05-17
WO2017100441A1 (en) 2017-06-15
AU2016366231A1 (en) 2018-06-14
EP3387152B1 (en) 2022-01-26
IL259788A (en) 2018-07-31
US20250313888A1 (en) 2025-10-09
JP2024010122A (ja) 2024-01-23

Similar Documents

Publication Publication Date Title
ES2911421T3 (es) Adaptadores, métodos y composiciones mejorados para secuenciación dúplex
ES2724824T3 (es) Métodos para la secuenciación de ácidos nucleicos
US20220064741A1 (en) High throughput nucleic acid sequencing by expansion
ES2866896T3 (es) Procedimiento para incrementar el rendimiento de secuenciación de molécula única concatenando fragmentos de ADN cortos
CN105283558B (zh) 使用热稳定的TthPrimPol扩增和测序的方法
ES2960184T3 (es) Códigos de barras de secuencias combinatorias para el cribado de alto rendimiento
BR122021026781B1 (pt) Transposon de preservação de contiguidade
EP3924504A1 (en) Haplotagging - haplotype phasing and single-tube combinatorial barcoding of nucleic acid molecules using bead-immobilized tn5 transposase
ES2965194T3 (es) Algoritmo de secuenciación
JP2025522572A (ja) 核酸配列決定のための方法及び組成物
US20190218606A1 (en) Methods of reducing errors in deep sequencing
CN103993100A (zh) 一种提高基因连接测序准确性的方法
CA3200114C (en) Rna probe for mutation profiling and use thereof
CN116685696A (zh) 从两端对多核苷酸片段进行测序的方法
EP3878972A1 (en) Barcoding technology for sequencing of nucleic acid molecules
EP4103749A1 (en) Reference ladders and adaptors
Tserovski Chemical Labeling and Next-generation Sequencing for Detection of RNA-modifications
WO2020012057A1 (es) Preparación de librerías de ácidos nucléicos o genotecas