JP6599592B2 - α−グルコシダーゼ活性抑制剤 - Google Patents
α−グルコシダーゼ活性抑制剤 Download PDFInfo
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- JP6599592B2 JP6599592B2 JP2019516029A JP2019516029A JP6599592B2 JP 6599592 B2 JP6599592 B2 JP 6599592B2 JP 2019516029 A JP2019516029 A JP 2019516029A JP 2019516029 A JP2019516029 A JP 2019516029A JP 6599592 B2 JP6599592 B2 JP 6599592B2
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- extract
- glucosidase activity
- cells
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- food
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/13—Coniferophyta (gymnosperms)
- A61K36/15—Pinaceae (Pine family), e.g. pine or cedar
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Description
<1>抽出溶媒が含水アルコール(含水率:1質量%〜60質量%)である琥珀抽出物を含有することを特徴とするα-グルコシダーゼ活性抑制剤。
<2><1>に記載の剤を配合してなるα−グルコシダーゼ活性抑制用経口投与組成物。
<3>態様が医薬品、医薬部外品、飲食品又は食品添加物である<2>に記載のα-グルコシダーゼ活性抑制用経口投与組成物。
本発明のα-グルコシダーゼ活性抑制剤は、有効成分として琥珀抽出物を含有することを特徴とする。ここで、本発明で言う琥珀抽出物とは、琥珀そのもの、琥珀を粉砕、細切等加工した加工物、琥珀又はその加工物に溶媒を加え抽出した抽出物、抽出物から溶媒を除去した抽出物の溶媒除去物、それらの精製物等が例示でき、これらの内では抽出物又はその溶媒除去物が特に好ましい。
<製造例1>
琥珀の粉末100gを50%エタノールで抽出し、それを減圧濃縮、凍結乾燥し、50%エタノール抽出物10gを得た。
<実施例1>スクラーゼ、マルターゼ活性抑制試験
本試験はOGAWAらの方法(N.Ogawaら, The Journal Of Nutrition, 130:507−513, 2000)を参考にした。
DMEM培地に、Caco−2細胞を懸濁し、1.0×104 cells/cm2となるように直径4cmのディッシュ(TPP社製)に播種した。95%空気−5%炭酸ガスの下、37℃で培養し、2から3日おきに培地交換を行いコンフルエントになるまで10日間培養した。培養上清を吸引除去後、製造例1の琥珀抽出物を終濃度100、200μg/mLになるようにDMSOに溶解させ、DMEM培地に混ぜたもの(DMSO終濃度:0.25%)を1mLずつ添加した。また、DMEM培地にDMSO終濃度0.25%になるようDMSOを添加した培養液を処理したものをcontrolとした。これらのディッシュを95%空気−5%炭酸ガスの下、37℃で7日間培養した。
<実施例3>SIタンパク発現抑制試験
DMEM培地に、Caco−2細胞を懸濁し、1.0×104cells/cm2となるように直径4cmのディッシュ(TPP社製)に播種した。95%空気−5%炭酸ガスの下、37℃で培養し、2〜3日おきに培地交換を行いコンフルエントになるまで10日間培養した。培養上清を吸引除去後、製造例1の琥珀抽出物を終濃度100、200μg/mLになるようにDMSOに溶解させ、DMEM培地に混ぜたもの(DMSO終濃度:0.25%)を1mLずつ添加した。また、DMEM培地にDMSO終濃度0.25%になるようDMSOを添加した培養液を処理したものをcontrolとした。これらのディッシュを95%空気−5%炭酸ガスの下、37℃で8日間培養した。
Claims (3)
- 抽出溶媒が含水率50質量%の含水エタノールである琥珀抽出物を含有することを特徴とするα−グルコシダーゼ活性抑制剤。
- 請求項1に記載の剤を配合してなるα−グルコシダーゼ活性抑制用経口投与組成物。
- 態様が医薬品、医薬部外品、飲食品又は食品添加物である請求項2に記載のα−グルコシダーゼ活性抑制用経口投与組成物。
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WO2019156213A1 (ja) * | 2018-02-09 | 2019-08-15 | 琥珀バイオテクノロジー株式会社 | グリセロール放出促進剤 |
JP6548797B1 (ja) * | 2018-08-10 | 2019-07-24 | 琥珀バイオテクノロジー株式会社 | アンジオテンシンi変換酵素活性阻害剤 |
JP2021088534A (ja) * | 2019-12-05 | 2021-06-10 | 琥珀バイオテクノロジー株式会社 | アディポネクチン産生促進剤 |
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JPH0662798U (ja) | 1993-02-10 | 1994-09-02 | 株式会社高岳製作所 | モータドライブ制御装置 |
JP4034839B2 (ja) | 1996-02-18 | 2008-01-16 | 希能 澤口 | 琥珀成分含有剤 |
JP4421718B2 (ja) | 1999-11-04 | 2010-02-24 | 希能 澤口 | 琥珀成分含有剤の製造方法 |
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JP4953204B2 (ja) * | 2006-04-25 | 2012-06-13 | 独立行政法人理化学研究所 | 琥珀から得られる皮膚ターンオーバー促進因子を含有する組成物及びその使用 |
FR2911779B1 (fr) * | 2007-01-30 | 2009-04-24 | Lvmh Rech | Composition contenant un extrait d'ambre |
JP4953203B2 (ja) | 2007-04-24 | 2012-06-13 | 独立行政法人理化学研究所 | 琥珀から得られるヒアルロン酸産生促進因子を含有する組成物及びその使用 |
JP5464730B2 (ja) | 2009-03-31 | 2014-04-09 | 丸善製薬株式会社 | 美白剤、抗老化剤、プロフィラグリンmRNA発現上昇促進剤、育毛剤及び毛乳頭細胞増殖促進剤 |
JP5590286B2 (ja) * | 2009-09-04 | 2014-09-17 | 国立大学法人岩手大学 | 新規なCa2+シグナル伝達阻害剤 |
JP5858561B2 (ja) * | 2010-05-11 | 2016-02-10 | 国立研究開発法人理化学研究所 | 琥珀由来のvegf産生促進剤及び琥珀由来のvegf産生促進剤を含む製剤又は化粧料若しくは頭髪・頭皮用化粧料 |
JP2012201570A (ja) * | 2011-03-28 | 2012-10-22 | Han-Tang Fu | 複合記憶材料と製造方法 |
JP5858562B2 (ja) * | 2011-05-19 | 2016-02-10 | 国立研究開発法人理化学研究所 | 琥珀から得られるエンドセリン−1産生抑制剤、scf産生抑制剤、及びメラニン産生抑制剤並びにその使用 |
CN105596776A (zh) * | 2015-12-30 | 2016-05-25 | 贾晓萍 | 一种治疗糖尿病的中药 |
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