JP6489017B2 - 核酸増幅法 - Google Patents

核酸増幅法 Download PDF

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Publication number
JP6489017B2
JP6489017B2 JP2015530853A JP2015530853A JP6489017B2 JP 6489017 B2 JP6489017 B2 JP 6489017B2 JP 2015530853 A JP2015530853 A JP 2015530853A JP 2015530853 A JP2015530853 A JP 2015530853A JP 6489017 B2 JP6489017 B2 JP 6489017B2
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JP
Japan
Prior art keywords
amino acid
seq
mutant
pcna
amplification
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Active
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JP2015530853A
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English (en)
Japanese (ja)
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JPWO2015019951A1 (ja
Inventor
哲大 小林
哲大 小林
弘嵩 松本
弘嵩 松本
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Toyobo Co Ltd
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Toyobo Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2015530853A 2013-08-06 2014-08-01 核酸増幅法 Active JP6489017B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2013162992 2013-08-06
JP2013162992 2013-08-06
JP2014061742 2014-03-25
JP2014061742 2014-03-25
PCT/JP2014/070322 WO2015019951A1 (ja) 2013-08-06 2014-08-01 核酸増幅法

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2018095268A Division JP6658796B2 (ja) 2013-08-06 2018-05-17 核酸増幅方法

Publications (2)

Publication Number Publication Date
JPWO2015019951A1 JPWO2015019951A1 (ja) 2017-03-02
JP6489017B2 true JP6489017B2 (ja) 2019-03-27

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JP2015530853A Active JP6489017B2 (ja) 2013-08-06 2014-08-01 核酸増幅法
JP2018095268A Active JP6658796B2 (ja) 2013-08-06 2018-05-17 核酸増幅方法

Family Applications After (1)

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JP2018095268A Active JP6658796B2 (ja) 2013-08-06 2018-05-17 核酸増幅方法

Country Status (2)

Country Link
JP (2) JP6489017B2 (https=)
WO (1) WO2015019951A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018161129A (ja) * 2013-08-06 2018-10-18 東洋紡株式会社 核酸増幅方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015122432A1 (ja) * 2014-02-17 2015-08-20 東洋紡株式会社 核酸増幅の正確性を向上させる方法
CN114891761B (zh) * 2022-03-25 2024-01-12 上海威高医疗技术发展有限公司 Tth DNA聚合酶突变体及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002360261A (ja) * 2001-06-11 2002-12-17 Toyobo Co Ltd Ｄｎａポリメラーゼ関連因子
US7960157B2 (en) * 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
JP4722035B2 (ja) * 2003-03-25 2011-07-13 アジレント・テクノロジーズ・インク Ｄｎａポリメラーゼ融合物およびその使用
KR20080031255A (ko) * 2005-07-04 2008-04-08 셀레스타 렉시코-사이언시즈 가부시키가이샤 변이형 ｐｃｎａ
US20070190538A1 (en) * 2005-07-29 2007-08-16 Applera Corporation Chimeric polymerases
JP6478444B2 (ja) * 2012-09-28 2019-03-06 東洋紡株式会社 改変された耐熱性ｄｎａポリメラーゼ
JP6119957B2 (ja) * 2012-10-12 2017-04-26 株式会社三洋物産 遊技機
JP6489017B2 (ja) * 2013-08-06 2019-03-27 東洋紡株式会社 核酸増幅法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018161129A (ja) * 2013-08-06 2018-10-18 東洋紡株式会社 核酸増幅方法

Also Published As

Publication number Publication date
JP6658796B2 (ja) 2020-03-04
JP2018161129A (ja) 2018-10-18
WO2015019951A1 (ja) 2015-02-12
JPWO2015019951A1 (ja) 2017-03-02

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