JP6482459B2 - 細胞サンプル中の生存細胞を検出する方法 - Google Patents

細胞サンプル中の生存細胞を検出する方法 Download PDF

Info

Publication number
JP6482459B2
JP6482459B2 JP2015510465A JP2015510465A JP6482459B2 JP 6482459 B2 JP6482459 B2 JP 6482459B2 JP 2015510465 A JP2015510465 A JP 2015510465A JP 2015510465 A JP2015510465 A JP 2015510465A JP 6482459 B2 JP6482459 B2 JP 6482459B2
Authority
JP
Japan
Prior art keywords
membrane
cells
viable cells
porous membrane
viable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2015510465A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015517656A5 (enExample
JP2015517656A (ja
Inventor
ウェインライト,ノーマン,アール.
マニアン,バラ,エス.
スティンプソン,エリック
コロニア,ブライアン,ジェイ.
コロニア,ロバート,ケイ.
Original Assignee
チャールズ リバー ラボラトリーズ, インコーポレイテッド
チャールズ リバー ラボラトリーズ, インコーポレイテッド
レアメトリックス インコーポレイテッド
レアメトリックス インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by チャールズ リバー ラボラトリーズ, インコーポレイテッド, チャールズ リバー ラボラトリーズ, インコーポレイテッド, レアメトリックス インコーポレイテッド, レアメトリックス インコーポレイテッド filed Critical チャールズ リバー ラボラトリーズ, インコーポレイテッド
Publication of JP2015517656A publication Critical patent/JP2015517656A/ja
Publication of JP2015517656A5 publication Critical patent/JP2015517656A5/ja
Application granted granted Critical
Publication of JP6482459B2 publication Critical patent/JP6482459B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6486Measuring fluorescence of biological material, e.g. DNA, RNA, cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2015510465A 2012-05-02 2013-05-02 細胞サンプル中の生存細胞を検出する方法 Expired - Fee Related JP6482459B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261641809P 2012-05-02 2012-05-02
US61/641,809 2012-05-02
US201361784789P 2013-03-14 2013-03-14
US61/784,789 2013-03-14
PCT/US2013/039349 WO2013166337A1 (en) 2012-05-02 2013-05-02 Method of detecting viable cells in a cell sample

Publications (3)

Publication Number Publication Date
JP2015517656A JP2015517656A (ja) 2015-06-22
JP2015517656A5 JP2015517656A5 (enExample) 2016-06-23
JP6482459B2 true JP6482459B2 (ja) 2019-03-13

Family

ID=48670053

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015510465A Expired - Fee Related JP6482459B2 (ja) 2012-05-02 2013-05-02 細胞サンプル中の生存細胞を検出する方法

Country Status (5)

Country Link
US (2) US9709500B2 (enExample)
EP (1) EP2780707B1 (enExample)
JP (1) JP6482459B2 (enExample)
CN (1) CN104755931B (enExample)
WO (1) WO2013166337A1 (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6482459B2 (ja) 2012-05-02 2019-03-13 チャールズ リバー ラボラトリーズ, インコーポレイテッド 細胞サンプル中の生存細胞を検出する方法
CN104685346B (zh) 2012-05-02 2018-05-29 查尔斯河实验室公司 细胞捕获系统及其使用
US8993260B2 (en) 2012-05-02 2015-03-31 Charles River Laboratories, Inc. Fluorescence-based viability staining method using a membrane permeable flourescent dye and membrane impermeable fluorescence quencher
USD719667S1 (en) * 2013-03-14 2014-12-16 Reametrix, Inc. Cell detection system
USD719666S1 (en) * 2013-03-14 2014-12-16 Reametrix, Inc. Cell detection system with touchscreen
USD717460S1 (en) * 2013-03-14 2014-11-11 Reametrix, Inc. Cell detection system
EP2976434B1 (en) * 2013-03-18 2017-08-02 Life Technologies Corporation Methods and systems for analyzing biological reaction systems
WO2015066727A1 (en) * 2013-11-04 2015-05-07 Charles River Laboratories, Inc. Method for detecting viable cells in a cell sample
USD724755S1 (en) 2013-11-04 2015-03-17 Charles River Laboratories, Inc. Cup
EP3066189B1 (en) 2013-11-04 2019-09-18 Charles River Laboratories, Inc. Filtration system and use thereof
US20150218612A1 (en) * 2014-02-06 2015-08-06 Tacount Exact Ltd. Apparatus, system and method for live bacteria microscopy
US9436866B2 (en) 2014-08-01 2016-09-06 General Electric Company High sensitivity flat panel microbiology detection and enumeration system
USD782694S1 (en) 2014-11-04 2017-03-28 Charles River Laboratories, Inc. Filtration device
USD776295S1 (en) 2014-11-04 2017-01-10 Charles River Laboratories, Inc. Base
USD759836S1 (en) 2014-11-04 2016-06-21 Charles River Laboratories, Inc. Cup
USD776296S1 (en) 2014-11-04 2017-01-10 Charles River Laboratories, Inc. Adapter
CN114723741B (zh) * 2015-04-23 2025-05-23 Bd科斯特公司 用于自动计数微生物菌落的方法和系统
USD817509S1 (en) * 2016-10-14 2018-05-08 Spartan Bioscience Inc. Diagnostic device
JP2018183088A (ja) * 2017-04-26 2018-11-22 アズビル株式会社 細胞生存率判定装置
WO2019051272A1 (en) * 2017-09-07 2019-03-14 Professional Compounding Centers Of America, Inc. METHOD AND SYSTEM FOR FLUORESCENCE DETECTION BY CYTOMETRY OF FLOWS OF REACTIVE MATERIALS IN NON-FILTRABLE VISCOUS MATERIALS
GB201716883D0 (en) * 2017-10-13 2017-11-29 Q-Linea Ab Method for determining the concentration of intact microorganisms in a sample
US10564100B1 (en) * 2018-10-12 2020-02-18 SageMedic Corporation Analysis of viable and nonviable cells
EP4492116A3 (en) 2018-11-21 2025-01-29 Cornell University Method of optical clearing of biological tissues
CN113196286A (zh) * 2018-12-21 2021-07-30 环球生命科技咨询美国有限责任公司 用于细胞培养监测的过程中装置和方法
KR102205473B1 (ko) * 2018-12-21 2021-01-21 칸트사이언스 주식회사 세포 내 pH 측정방법
US20230294097A1 (en) * 2020-05-04 2023-09-21 The Regents Of The University Of California Electrochemical cellular circuits
CN113670696A (zh) * 2021-07-28 2021-11-19 上海睿钰生物科技有限公司 一种用于细胞染色的染色液及其配制方法
TWI866690B (zh) * 2023-02-07 2024-12-11 邦睿生技股份有限公司 生物樣品檢測裝置

Family Cites Families (126)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1256360A (en) 1984-02-10 1989-06-27 John W. Bornhoeft Apparatus and method for determining the effectiveness of sterlization
US4561553A (en) 1985-01-22 1985-12-31 Northern Engineering And Plastics Corp. Snap on twist off tamper-proof closure for containers
EP0215066A1 (en) 1985-02-27 1987-03-25 University Of Cincinnati Viable microorganism detection by induced fluorescence
US4725891A (en) 1986-09-15 1988-02-16 Matrix Instruments Inc. Film image digitizer
US4739416A (en) 1986-09-15 1988-04-19 Matrix Instruments Inc. Digital image reproduction
US4783401A (en) 1986-10-31 1988-11-08 Smithkline Beckman Corporation Viable cell labelling
US5037656A (en) 1986-12-04 1991-08-06 Millipore Corporation Porous membrane having hydrophilic and cell growth promotions surface and process
JPH0813930B2 (ja) 1987-12-28 1996-02-14 三井東圧化学株式会社 近赤外線吸収フィルター用高純度アントラキノン系色素
US4930893A (en) 1988-03-25 1990-06-05 Molecular Dynamics Electrophoresis imaging system
US4838632A (en) 1988-05-06 1989-06-13 Lumisys Inc. Two-dimensional beam scanner
US5073488A (en) 1988-11-29 1991-12-17 Minnesota Mining And Manufacturing Company Rapid method for determining efficacy of a sterilization cycle and rapid read-out biological indicator
WO1992002632A1 (en) * 1990-07-30 1992-02-20 Sierra Cytometry Fluorescent dyes for identification and enumeration of viable cells in milk
US5185450A (en) 1991-02-19 1993-02-09 University Of South Florida Tetrazolium compounds for cell viability assays
US5172419A (en) 1991-03-05 1992-12-15 Lumisys, Inc. Medical image processing system
US5296341A (en) 1991-03-05 1994-03-22 Lumisys, Inc. Green light laser imaging method
US5314805A (en) 1991-10-28 1994-05-24 Molecular Probes, Inc. Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM
US5234585A (en) 1991-12-19 1993-08-10 Zuk, Incorporated Vacuum filtration device
US5137609A (en) 1992-01-31 1992-08-11 Biometric Imaging Inc. Differential separation assay
US5843680A (en) 1992-01-31 1998-12-01 Biometric Imaging, Inc. Differential separation assay methods and test kits
US5221454A (en) 1992-01-31 1993-06-22 Biometric Imaging Inc. Differential separation assay
DE69311944T2 (de) 1992-04-01 1997-11-20 Nihon Millipore Kogyo K K Verfahren zur Bestimmung der Anzahl lebender Mikroorganismen
JP3228812B2 (ja) 1993-02-10 2001-11-12 日本マイクロリス株式会社 生菌数を測定する方法
US5585246A (en) 1993-02-17 1996-12-17 Biometric Imaging, Inc. Method for preparing a sample in a scan capillary for immunofluorescent interrogation
US5547849A (en) 1993-02-17 1996-08-20 Biometric Imaging, Inc. Apparatus and method for volumetric capillary cytometry
US5545535A (en) 1993-04-13 1996-08-13 Molecular Probes, Inc. Fluorescent assay for bacterial gram reaction
US5534416A (en) 1993-04-13 1996-07-09 Molecular Probes, Inc. Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes
US5445946A (en) 1993-04-13 1995-08-29 Molecular Probes, Inc. Intravacuolar stains for yeast and other fungi
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5437980A (en) 1993-05-17 1995-08-01 Molecular Probes, Inc. Phenanthridium dye staining of nucleic acids in living cells
FR2707665A1 (fr) 1993-06-28 1995-01-20 Chemunex Procédé de mesure et d'évaluation de la vitalité des microorganismes et ses applications.
JP3011036B2 (ja) 1993-12-20 2000-02-21 三菱化学株式会社 光ディスク基板の複屈折の測定法
IES940182A2 (en) * 1994-03-01 1995-11-29 Teagasc Agric Food Dev Authori "Rapid detection of bacteria in liquid cultures"
JP3137160B2 (ja) 1994-04-01 2001-02-19 日立電子エンジニアリング株式会社 ウエハの位置ズレ補正方法
US5550032A (en) 1994-05-27 1996-08-27 George Mason University Biological assay for microbial contamination
JP3593161B2 (ja) 1994-11-15 2004-11-24 株式会社トプコン 回転体上異物位置測定装置
EP0713087B1 (en) 1994-11-17 1999-04-14 Chemunex Apparatus and process for rapid and ultrasensitive detection and counting of microorganisms by fluorescence
US5603900A (en) 1995-05-19 1997-02-18 Millipore Investment Holdings Limited Vacuum filter device
US5565678A (en) 1995-06-06 1996-10-15 Lumisys, Inc. Radiographic image quality assessment utilizing a stepped calibration target
WO1997012226A1 (en) 1995-09-25 1997-04-03 Tencor Instruments Improved system for surface inspection
HUP9902028A2 (hu) 1995-11-08 1999-11-29 Pioneer Hi-Bred International, Inc. Eljárások mikrobatenyészetek életképességének becslésére
US20020119438A1 (en) 1995-12-29 2002-08-29 Fuminori Kato Method for determining viable cell count
US6459805B1 (en) 1996-03-26 2002-10-01 Childrens Hospital Los Angeles Fluorescence digital imaging microscopy system
DE19621312A1 (de) 1996-05-28 1997-12-04 Bayer Ag Maskierung der Hintergrundfluoreszenz und Signalverstärkung bei der optischen Analyse biologisch medizinischer Assays
US5956146A (en) 1997-01-29 1999-09-21 Victor Company Of Japan, Ltd. Birefringence measuring apparatus for optical disc substrate
US6221621B1 (en) 1997-03-06 2001-04-24 Bard Diagnostic Sciences, Inc. Methods of screening for colorectal cancers in which a complement Factor I or related protein is associated
FR2764305B1 (fr) 1997-06-04 2000-10-06 Chemunex Procede de detection et de numeration de cellules viables dans un echantillon biologique et kit pour sa mise en oeuvre
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6214563B1 (en) 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6043506A (en) 1997-08-13 2000-03-28 Bio-Rad Laboratories, Inc. Multi parameter scanner
US5922617A (en) 1997-11-12 1999-07-13 Functional Genetics, Inc. Rapid screening assay methods and devices
WO1999035499A1 (en) 1997-12-30 1999-07-15 Remacle Jose Method comprising capture molecule fixed on disc surface
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
JP2002521690A (ja) 1998-07-28 2002-07-16 バイオメトリック イメージング インコーポレイテッド 細胞運動性アッセイのための装置および方法
US6130745A (en) 1999-01-07 2000-10-10 Biometric Imaging, Inc. Optical autofocus for use with microtiter plates
JP2000232897A (ja) * 1999-02-15 2000-08-29 Nippon Mizushori Giken:Kk 菌類の即時判別方法
EP1314980B1 (en) 1999-02-26 2009-12-09 Cellomics, Inc. A system for cell-based screening
JP2000304688A (ja) 1999-04-16 2000-11-02 Canon Inc 基板測定方法および装置
US6181413B1 (en) 1999-09-03 2001-01-30 Biometric Imaging, Inc. Displacing volume in field of view
ATE399878T1 (de) 1999-10-25 2008-07-15 Genprime Inc Verfahren und vorrichtung zur quantifizierung von prokaryotischen und eukaryotischen zellen
JP2001228088A (ja) 2000-02-18 2001-08-24 Nippon Laser & Electronics Lab 生体試料光学的走査装置
CA2337987A1 (en) 2000-02-25 2001-08-25 Elmex Limited Membrane filtration system
JP2001242082A (ja) 2000-02-29 2001-09-07 Nippon Laser & Electronics Lab 生体試料光学的走査装置
GB0008787D0 (en) 2000-04-10 2000-05-31 Norsk Naeringsmiddelforskning A method of cell detection
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
IL139593A (en) 2000-11-09 2010-12-30 Biogem Optical Ltd Method for the detection of viable microorganisms
US7546925B1 (en) 2000-12-04 2009-06-16 Roush Life Sciences, Llc Disposable vacuum filtration apparatus capable of detecting microorganisms and particulates in liquid samples
US6633368B2 (en) 2001-01-02 2003-10-14 Becton, Dickinson And Company Method for determining the volume of single red blood cells
US6714287B2 (en) 2001-01-02 2004-03-30 Becton, Dickinson And Company Apparatus for determining the volume of single red blood cells
CA2445509A1 (en) 2001-01-25 2002-12-19 James D. Thacker Rapid methods for microbial typing and enumeration
JP2002310886A (ja) 2001-04-11 2002-10-23 Canon Inc ディスクサイトメトリーによる分析方法及び装置
US7016087B2 (en) 2001-08-08 2006-03-21 Becton Dickinson And Company Photon efficient scanner
US6750457B2 (en) 2001-08-29 2004-06-15 Becton Dickinson And Company System for high throughput analysis
CA2458802C (en) 2001-09-06 2016-06-07 Genomic Profiling Systems, Inc. Rapid and sensitive detection of molecules
US7205100B2 (en) 2002-10-10 2007-04-17 Advanced Analytical Technologies, Inc. Methods for reducing background fluorescence
JP4231920B2 (ja) * 2003-08-01 2009-03-04 独立行政法人産業技術総合研究所 ディスク状バイオチップ及びその読取り装置
US7435576B2 (en) 2003-09-30 2008-10-14 Gen-Probe Incorporated Filter snapper
ES2239886B1 (es) 2003-11-05 2006-12-16 Universidad De Barcelona Metodo y aparato para la determinacion de la viabilidad celular.
EP1699351B1 (en) 2003-12-22 2011-05-04 Lightouch Medical, Inc. Process for determination of cell viability
WO2005088287A1 (en) 2004-03-17 2005-09-22 National Research Council Of Canada Method and apparatus for the detection of microorganisms
DE102004019685A1 (de) 2004-04-20 2005-11-17 DRäGER AEROSPACE GMBH Passagiersauerstoffmaske
US20060129327A1 (en) 2004-07-29 2006-06-15 Kim Myung L Ultrasensitive sensor and rapid detection of analytes
KR20070061802A (ko) 2004-07-29 2007-06-14 킴 래버러터리즈, 아이엔씨. 초음파 감응 센서 및 분석 대상물의 고속 검출 방법
DE602005010515D1 (de) 2004-08-06 2008-12-04 Fuji Electric Holdings Nachweisverfahren für lebenfähige Zellen
JP4779433B2 (ja) 2004-08-26 2011-09-28 パナソニック株式会社 ろ過フィルタデバイス
US20060121443A1 (en) 2004-12-03 2006-06-08 Orion Biosolutions, Inc. Determination of cell viability
US7018804B1 (en) 2004-12-03 2006-03-28 Orion Biosolutions, Inc. Determination of cell viability and phenotype
US20090170144A1 (en) 2005-02-22 2009-07-02 Heineman William R Determination of viable microorganisms using coated paramagnetic beads
JP2007033170A (ja) * 2005-07-26 2007-02-08 Fujifilm Corp 蛍光検出方法および蛍光検出システム
EP1919622B1 (en) 2005-09-02 2013-08-14 Nypro Inc. Systems, apparatus and methods for vacuum filtration
US8268567B2 (en) 2005-09-16 2012-09-18 The Charles Stark Draper Laboratory Reaction sensing in living cells
JP5268175B2 (ja) 2006-01-12 2013-08-21 バイオセンス テクノロジーズ インク. 迅速に細胞の生存テストを実行するための方法及び構成物
DE102006022877B4 (de) 2006-05-15 2009-01-29 Sartorius Stedim Biotech Gmbh Verfahren zur Bestimmung der Viabilität von Zellen in Zellkulturen
GB0615302D0 (en) 2006-08-01 2006-09-13 Biotrace Internat Plc Assay Method For The Detection Of Viable Microbial Cells In A Sample
US20080153125A1 (en) 2006-11-10 2008-06-26 Advanced Analytical Technologies, Inc. Rapid detection of microorganisms in fluids
CN101201312A (zh) 2006-12-15 2008-06-18 北京林业大学 一种中性红染色法检测藻细胞活性的方法
EP2142665B1 (en) 2007-04-13 2013-11-27 Promega Corporation Luminescent live and dead cell assay
US20080305514A1 (en) * 2007-06-06 2008-12-11 Alcon Research, Ltd. Method for detecting microbes
WO2009029039A1 (en) 2007-08-28 2009-03-05 Ridgeview Instruments Ab Method for measurement of cell viability
US20090099525A1 (en) 2007-10-11 2009-04-16 Animas Corporation Drug Delivery System with Breakaway Plunger Extractor
US8216778B2 (en) 2007-11-09 2012-07-10 Genzyme Corporation Methods of measuring cell viability in tissue engineered products
US8986946B2 (en) 2007-12-20 2015-03-24 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Real-time method for the detection of viable micro-organisms
US20110110861A1 (en) 2008-05-13 2011-05-12 Mireille Hanna Lahoud Methods of Detecting Cells with a Disrupted Cell Membrane, Cells Infected with A Pathogen, Dying Cells or Dead Cells
US9128097B2 (en) 2008-07-14 2015-09-08 Chemometec A/S Method and kit for assessing viable cells
CN101344476B (zh) 2008-08-20 2011-03-16 吉林农业大学 细菌活的非可培养状态荧光显微镜观察与计数方法
CN102414324B (zh) 2009-05-04 2015-12-02 诺华股份有限公司 快速无菌微测试
FR2945457B1 (fr) 2009-05-12 2011-07-15 Aes Chemunex Ensemble forme d'une membrane de filtration et d'une plaque de support, ainsi que dispositif de test microbiologique en faisant usage.
US8148515B1 (en) 2009-06-02 2012-04-03 Biotium, Inc. Detection using a dye and a dye modifier
WO2010151131A2 (en) 2009-06-26 2010-12-29 Nederlandse Organisatie Voor Toegepast- Natuurwetenschappelijk Onderzoek Tno Apparatus for detecting viable microorganisms or spores in a sample and use thereof.
CA2768699C (en) 2009-07-24 2017-08-22 Morinaga Milk Industry Co., Ltd. Method and kit for detection of live microorganisms
CA2781516C (en) 2010-03-26 2016-01-26 Morinaga Milk Industry Co., Ltd. Culture medium for specific growth, detection, and enumeration of bifidobacterium breve
GB201005939D0 (en) 2010-04-09 2010-05-26 Biostatus Ltd Method of analysing a cell or other biological material containing nucleic acid
US20110294206A1 (en) * 2010-05-28 2011-12-01 California Institute Of Technology Methods and design of membrane filters
ES2646413T3 (es) 2010-06-03 2017-12-13 Basf Se Detección y recuento de microorganismos
US20130210063A1 (en) 2010-10-13 2013-08-15 Life Technologies Corporation Compositions and assays for determining cell viability
CA2816014A1 (en) 2010-11-03 2012-05-10 Reametrix Inc. Method and device for fluorescent measurement of samples
CA2816017A1 (en) 2010-11-03 2012-05-10 Reametrix Inc. Methods for calibrating a raman spectrometer, and spectrometer
CA2815951A1 (en) 2010-11-03 2012-05-10 Reametrix Inc. Measurement system for fluorescent detection, and method therefor
EP2637787A1 (en) 2010-11-08 2013-09-18 Reactrix Systems, Inc. Sample assembly for a measurement device
US9446411B2 (en) 2011-02-24 2016-09-20 Reametrix, Inc. Sample assembly for a measurement device
US20130068310A1 (en) 2011-09-15 2013-03-21 University Of Washington Through Its Center Of Commercialization Method and Apparatus for a Microfluidic Device
CN104685346B (zh) 2012-05-02 2018-05-29 查尔斯河实验室公司 细胞捕获系统及其使用
US8993260B2 (en) 2012-05-02 2015-03-31 Charles River Laboratories, Inc. Fluorescence-based viability staining method using a membrane permeable flourescent dye and membrane impermeable fluorescence quencher
JP6482459B2 (ja) 2012-05-02 2019-03-13 チャールズ リバー ラボラトリーズ, インコーポレイテッド 細胞サンプル中の生存細胞を検出する方法

Also Published As

Publication number Publication date
US9709500B2 (en) 2017-07-18
EP2780707A1 (en) 2014-09-24
CN104755931A (zh) 2015-07-01
WO2013166337A1 (en) 2013-11-07
CN104755931B (zh) 2016-08-31
EP2780707B1 (en) 2016-02-24
JP2015517656A (ja) 2015-06-22
US20180120231A1 (en) 2018-05-03
US20130323745A1 (en) 2013-12-05

Similar Documents

Publication Publication Date Title
JP6482459B2 (ja) 細胞サンプル中の生存細胞を検出する方法
JP6285915B2 (ja) 生死判別染色法
JP6240162B2 (ja) 細胞収集システムおよびその使用
AU2007207450B2 (en) Method for rapid detection and evaluation of cultured cell growth
KR20070061802A (ko) 초음파 감응 센서 및 분석 대상물의 고속 검출 방법
Connally A device for gated autosynchronous luminescence detection
CN114057604A (zh) 一种细胞核荧光染料及其染色方法
US20160298162A1 (en) Method for detecting viable cells in a cell sample
Leader et al. DEVELOPING AN OXGYEN DETECTION METHOD FOR A MICROFLUIDIC-BASED HYPOXIA CHAMBER

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160426

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160426

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20170329

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170407

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170707

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170907

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171006

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20180223

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20180523

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20180720

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180817

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20190129

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20190212

R150 Certificate of patent or registration of utility model

Ref document number: 6482459

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees