JP6473510B2 - ビブリオで汎用される遺伝子ノックアウト用の自殺ベクターおよびその利用 - Google Patents

ビブリオで汎用される遺伝子ノックアウト用の自殺ベクターおよびその利用 Download PDF

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JP6473510B2
JP6473510B2 JP2017536525A JP2017536525A JP6473510B2 JP 6473510 B2 JP6473510 B2 JP 6473510B2 JP 2017536525 A JP2017536525 A JP 2017536525A JP 2017536525 A JP2017536525 A JP 2017536525A JP 6473510 B2 JP6473510 B2 JP 6473510B2
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vibrio
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suicide vector
suicide
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鵬 羅
鵬 羅
香燕 何
香燕 何
秋▲ティン▼ 劉
秋▲ティン▼ 劉
超群 胡
超群 胡
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South China Sea Institute of Oceanology of CAS
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    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
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    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C12N15/03Bacteria
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/63Vibrio

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JP2017536525A 2015-09-16 2015-12-11 ビブリオで汎用される遺伝子ノックアウト用の自殺ベクターおよびその利用 Active JP6473510B2 (ja)

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Application Number Priority Date Filing Date Title
CN201510593592.7A CN105063073A (zh) 2015-09-16 2015-09-16 一种弧菌通用的基因敲除自杀载体及其应用
CN201510593592.7 2015-09-16
PCT/CN2015/097202 WO2017045281A1 (zh) 2015-09-16 2015-12-11 一种弧菌通用的基因敲除自杀载体及其应用

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JP2018500920A JP2018500920A (ja) 2018-01-18
JP6473510B2 true JP6473510B2 (ja) 2019-02-20

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Publication number Priority date Publication date Assignee Title
CN105063073A (zh) * 2015-09-16 2015-11-18 中国科学院南海海洋研究所 一种弧菌通用的基因敲除自杀载体及其应用
CN105505974B (zh) * 2016-01-21 2019-01-11 浙江大学 一种实现大肠杆菌基因无痕同源重组的方法
CN106399213A (zh) * 2016-08-09 2017-02-15 江苏省农业科学院 一种抗生素溶杆菌基因敲除系统及其构建方法和应用
CN106834332B (zh) * 2017-03-09 2021-05-04 中国科学院过程工程研究所 一种重组质粒及其制备方法和应用
CN109517844B (zh) * 2018-10-17 2021-04-30 中国水产科学研究院东海水产研究所 一种鳗弧菌鞭毛蛋白基因敲除的方法
CN109880844A (zh) * 2019-03-06 2019-06-14 江苏省农业科学院 一种基于同源重组的变棕溶杆菌oh23基因敲除系统的构建方法与应用
CN110066820B (zh) * 2019-04-29 2021-03-16 华南农业大学 一种荧光菌株E.coli C600及构建方法与应用
CN110591993A (zh) * 2019-09-09 2019-12-20 中国水产科学研究院南海水产研究所 一种基于盐酸刺激的哈维弧菌同源重组基因敲除的方法
CN110591994B (zh) * 2019-09-09 2023-10-20 中国水产科学研究院南海水产研究所 一种基于氢氧化钠刺激的哈维弧菌同源重组基因敲除的方法

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GB0121998D0 (en) * 2001-09-11 2001-10-31 Acambis Res Ltd Attenuated bacteria useful in vaccines
CN104817632B (zh) * 2015-05-05 2018-03-02 中国科学院南海海洋研究所 拟态弧菌ta系统毒素蛋白及其编码基因和应用
CN105063073A (zh) * 2015-09-16 2015-11-18 中国科学院南海海洋研究所 一种弧菌通用的基因敲除自杀载体及其应用

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JP2018500920A (ja) 2018-01-18
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