JP6364074B2 - マルチビュー・ライトシート顕微鏡検査法 - Google Patents
マルチビュー・ライトシート顕微鏡検査法 Download PDFInfo
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- JP6364074B2 JP6364074B2 JP2016521691A JP2016521691A JP6364074B2 JP 6364074 B2 JP6364074 B2 JP 6364074B2 JP 2016521691 A JP2016521691 A JP 2016521691A JP 2016521691 A JP2016521691 A JP 2016521691A JP 6364074 B2 JP6364074 B2 JP 6364074B2
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/10—Condensers affording dark-field illumination
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Optics & Photonics (AREA)
- Health & Medical Sciences (AREA)
- Multimedia (AREA)
- Engineering & Computer Science (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361888869P | 2013-10-09 | 2013-10-09 | |
| US61/888,869 | 2013-10-09 | ||
| PCT/US2014/059822 WO2015054450A1 (en) | 2013-10-09 | 2014-10-09 | Multiview light-sheet microscopy |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018124403A Division JP6625696B2 (ja) | 2013-10-09 | 2018-06-29 | マルチビュー・ライトシート顕微鏡検査法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016538584A JP2016538584A (ja) | 2016-12-08 |
| JP2016538584A5 JP2016538584A5 (enExample) | 2017-11-24 |
| JP6364074B2 true JP6364074B2 (ja) | 2018-07-25 |
Family
ID=52776741
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016521691A Active JP6364074B2 (ja) | 2013-10-09 | 2014-10-09 | マルチビュー・ライトシート顕微鏡検査法 |
| JP2018124403A Active JP6625696B2 (ja) | 2013-10-09 | 2018-06-29 | マルチビュー・ライトシート顕微鏡検査法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018124403A Active JP6625696B2 (ja) | 2013-10-09 | 2018-06-29 | マルチビュー・ライトシート顕微鏡検査法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US10539772B2 (enExample) |
| EP (1) | EP3055728B1 (enExample) |
| JP (2) | JP6364074B2 (enExample) |
| WO (1) | WO2015054450A1 (enExample) |
Families Citing this family (68)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014531908A (ja) | 2011-10-14 | 2014-12-04 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 構造アッセンブリによる配列決定 |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| WO2013184754A2 (en) | 2012-06-05 | 2013-12-12 | President And Fellows Of Harvard College | Spatial sequencing of nucleic acids using dna origami probes |
| US10138509B2 (en) | 2013-03-12 | 2018-11-27 | President And Fellows Of Harvard College | Method for generating a three-dimensional nucleic acid containing matrix |
| DE102013112596B4 (de) * | 2013-11-15 | 2023-12-28 | Carl Zeiss Microscopy Gmbh | Anordnung zur Lichtblattmikroskopie |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| ES2567379B1 (es) * | 2014-10-21 | 2017-02-03 | Universidad Carlos Iii De Madrid | Microscopio y procedimiento para la generación de imágenes 3D de una colección demuestras |
| DE102014116174A1 (de) * | 2014-11-06 | 2016-05-12 | Carl Zeiss Microscopy Gmbh | Verfahren zur Erzeugung eines Bildes einer Probe |
| US10795144B2 (en) * | 2014-12-06 | 2020-10-06 | Howard Hughes Medical Institute | Microscopy with structured plane illumination and point accumulation for imaging and nanoscale topography |
| CA2977073A1 (en) | 2015-02-23 | 2016-09-01 | Li-Cor, Inc. | Fluorescence biopsy specimen imager and methods |
| JP6552881B2 (ja) * | 2015-06-12 | 2019-07-31 | オリンパス株式会社 | 顕微鏡および顕微鏡画像取得方法 |
| CN107709968A (zh) | 2015-06-26 | 2018-02-16 | 利康公司 | 荧光活检样本成像仪及方法 |
| ITUB20154591A1 (it) * | 2015-10-12 | 2017-04-12 | Crestoptics S R L | Apparato di microscopia confocale e relativo procedimento di acquisizione ed elaborazione di immagini |
| US10802262B2 (en) * | 2015-10-29 | 2020-10-13 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and systems for imaging a biological sample |
| JP6882282B2 (ja) * | 2015-11-03 | 2021-06-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 三次元核酸含有マトリックスの立体撮像のための方法と装置 |
| CN113063755A (zh) * | 2015-11-17 | 2021-07-02 | 韩国科学技术院 | 利用混沌波传感器的样品特性探测装置 |
| DE102016204653B4 (de) * | 2016-03-21 | 2022-12-22 | Carl Zeiss Microscopy Gmbh | Die Erfindung betrifft ein Lichtblattmikroskop sowie ein Verfahren zum Betreiben eines Lichtblattmikroskops |
| DE102016105798A1 (de) * | 2016-03-30 | 2017-10-05 | Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts, Universitätsmedizin | Verfahren zum Erzeugen eines Mikroskopbildes |
| LU93022B1 (de) | 2016-04-08 | 2017-11-08 | Leica Microsystems | Verfahren und Mikroskop zum Untersuchen einer Probe |
| EP3446098A1 (en) | 2016-04-21 | 2019-02-27 | Li-Cor, Inc. | Multimodality multi-axis 3-d imaging |
| EP4613756A3 (en) | 2016-04-25 | 2025-11-12 | President And Fellows Of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
| DE102016108384B3 (de) * | 2016-05-04 | 2017-11-09 | Leica Microsystems Cms Gmbh | Vorrichtung und Verfahren zur lichtblattartigen Beleuchtung einer Probe |
| US10495554B2 (en) * | 2016-05-25 | 2019-12-03 | The Board Of Trustees Of The Leland Stanford Junior University | Method and system for imaging and analysis of a biological specimen |
| WO2017223378A1 (en) | 2016-06-23 | 2017-12-28 | Li-Cor, Inc. | Complementary color flashing for multichannel image presentation |
| US11320640B2 (en) * | 2016-06-24 | 2022-05-03 | Howard Hughes Medical Institute | Automated adjustment of light sheet geometry in a microscope |
| EP3497502B1 (de) * | 2016-08-15 | 2022-10-26 | Leica Microsystems CMS GmbH | Lichtblattmikroskop |
| EP4273594A3 (en) | 2016-09-09 | 2024-01-10 | The University of North Carolina at Chapel Hill | Tilted illumination systems for fluoresence microscopes |
| EP3296723A1 (en) * | 2016-09-14 | 2018-03-21 | ASML Netherlands B.V. | Illumination source for an inspection apparatus, inspection apparatus and inspection method |
| US10690898B2 (en) * | 2016-09-15 | 2020-06-23 | Molecular Devices (Austria) GmbH | Light-field microscope with selective-plane illumination |
| US11506877B2 (en) * | 2016-11-10 | 2022-11-22 | The Trustees Of Columbia University In The City Of New York | Imaging instrument having objective axis and light sheet or light beam projector axis intersecting at less than 90 degrees |
| WO2018098162A1 (en) | 2016-11-23 | 2018-05-31 | Li-Cor, Inc. | Motion-adaptive interactive imaging method |
| JP2018105936A (ja) * | 2016-12-22 | 2018-07-05 | オリンパス株式会社 | 顕微鏡、設定支援方法 |
| CN110100175A (zh) * | 2017-02-02 | 2019-08-06 | 伊斯梅卡半导体控股公司 | 用于检查部件的组件和方法 |
| US11156822B2 (en) | 2017-04-24 | 2021-10-26 | Igor Lyuboshenko | Selective plane illumination microscopy with multiple illumination units scanning an object in sync with a digital camera rolling shutter |
| US10768400B2 (en) | 2017-04-24 | 2020-09-08 | Igor Lyuboshenko | Varying an illumination path of a selective plane illumination microscopy |
| US10365464B1 (en) | 2017-04-24 | 2019-07-30 | Igor Lyuboshenko | Extending optical microscopes to provide selective plane illumination microscopy |
| US12474561B2 (en) | 2017-04-24 | 2025-11-18 | Igor Lyuboshenko | Automatic correction of spherical aberration in selective plane illumination microscopy |
| EP3616158A1 (en) | 2017-04-25 | 2020-03-04 | Li-Cor, Inc. | Top-down and rotational side view biopsy specimen imager and methods |
| JP7014794B2 (ja) * | 2017-07-11 | 2022-02-01 | 浜松ホトニクス株式会社 | 試料観察装置及び試料観察方法 |
| EP3454105A1 (en) * | 2017-09-08 | 2019-03-13 | Igor Lyuboshenko | Varying an illumination path of a selective plane illumination microscopy |
| CN107884379A (zh) * | 2017-12-18 | 2018-04-06 | 北京赛尔怡美科技有限公司 | 光片照明成像装置 |
| CN111869205B (zh) * | 2018-01-19 | 2022-06-10 | Pcms控股公司 | 具有变化位置的多焦平面 |
| CN108334688B (zh) * | 2018-01-29 | 2022-04-01 | 南京理工大学 | 基于matlab的旋转功能梯度厚板动力学行为计算方法 |
| WO2019152216A1 (en) * | 2018-02-01 | 2019-08-08 | University Of Pittsburgh-Of The Commonwealth System Of Higher Education | Systems and methods for robust background correction and/or emitter localization for super-resolution localization microscopy |
| CN112005100B (zh) * | 2018-03-12 | 2024-09-20 | 查珀尔希尔北卡罗来纳大学 | 用于荧光显微镜的光盘显微术 |
| CN112020641B (zh) | 2018-03-12 | 2024-04-16 | 查珀尔希尔北卡罗来纳大学 | 用于增加收集的镜像显微术 |
| US12153227B2 (en) | 2018-03-12 | 2024-11-26 | The University Of North Carolina At Chapel Hill | Light disc microscopy for fluorescence microscopes |
| US11477434B2 (en) | 2018-03-23 | 2022-10-18 | Pcms Holdings, Inc. | Multifocal plane based method to produce stereoscopic viewpoints in a DIBR system (MFP-DIBR) |
| CN108956562B (zh) * | 2018-06-11 | 2020-05-22 | 浙江大学 | 一种基于重定位的光切片荧光显微成像方法和装置 |
| WO2020010018A1 (en) | 2018-07-05 | 2020-01-09 | Pcms Holdings, Inc. | Method and system for near-eye focal plane overlays for 3d perception of content on 2d displays |
| DE102018215833B4 (de) * | 2018-09-18 | 2020-04-02 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Optische Anordnung für fluoreszenzmikroskopische Anwendungen |
| DE102018215831B4 (de) * | 2018-09-18 | 2020-04-02 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Optische Anordnung für fluoreszenzmikroskopische Anwendungen |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| WO2020161826A1 (ja) | 2019-02-06 | 2020-08-13 | オリンパス株式会社 | 撮像装置 |
| US11187659B2 (en) * | 2019-03-28 | 2021-11-30 | The Regents Of The University Of California | Systems for fluorescence light sheet microscopy of large samples in high refractive index solutions |
| EP3796065B1 (en) * | 2019-09-20 | 2025-02-19 | Leica Microsystems CMS GmbH | Light sheet microscope with exchangeable optical elements |
| CN111521608A (zh) * | 2020-04-27 | 2020-08-11 | 中国科学院广州生物医药与健康研究院 | 一种超分辨率显微成像方法及显微镜 |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| KR102451422B1 (ko) * | 2020-11-24 | 2022-10-07 | 울산과학기술원 | 초고해상도 이미지와 고속 광시야 이미지를 동시에 획득하는 방법 및 장치 |
| EP4036628A1 (en) * | 2021-01-22 | 2022-08-03 | Viventis Microscopy Sàrl | Microscope for imaging a sample |
| DE102021107821B4 (de) * | 2021-03-29 | 2023-05-11 | Universität Kassel, Körperschaft des öffentlichen Rechts | Lichtblatt-fluoreszenzmikroskop und verfahren zur tomographischen darstellung |
| US12443023B2 (en) * | 2021-05-14 | 2025-10-14 | Igor Lyuboshenko | Light sheet microscope having streamlined field of view changes |
| JPWO2022249583A1 (enExample) * | 2021-05-26 | 2022-12-01 | ||
| EP4134724A1 (en) * | 2021-08-13 | 2023-02-15 | European Molecular Biology Laboratory | Inverted light-sheet microscope |
| WO2023049164A1 (en) * | 2021-09-22 | 2023-03-30 | Howard Hughes Medical Institute | Multiscale multiview light-sheet imaging |
| CN114236801B (zh) * | 2021-10-25 | 2024-01-05 | 北京京东方技术开发有限公司 | 光片生成装置及具有其的显微镜系统 |
| WO2024068455A1 (en) * | 2022-09-26 | 2024-04-04 | Consiglio Nazionale Delle Ricerche | System and method for multicolor light sheet microscopy |
| WO2024178045A1 (en) * | 2023-02-21 | 2024-08-29 | The Board Of Regents Of The University Of Texas System | Systems, methods, and devices providing fluorescence microscopy confocally sectioned images |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003185927A (ja) | 2001-12-13 | 2003-07-03 | Olympus Optical Co Ltd | 走査型レーザー顕微鏡 |
| DE10257423A1 (de) | 2002-12-09 | 2004-06-24 | Europäisches Laboratorium für Molekularbiologie (EMBL) | Mikroskop |
| WO2009002225A2 (ru) | 2007-06-25 | 2008-12-31 | Closed Company 'molecular-Medicine Technologies' | Многофункциональное устройство для диагностики и способ тестирования биологических объектов |
| DE102007045897A1 (de) | 2007-09-26 | 2009-04-09 | Carl Zeiss Microimaging Gmbh | Verfahren zur mikroskopischen dreidimensionalen Abbildung einer Probe |
| DE102007063274B8 (de) | 2007-12-20 | 2022-12-15 | Albert-Ludwigs-Universität Freiburg | Mikroskop |
| DE102008009216A1 (de) | 2008-02-13 | 2009-08-20 | Carl Zeiss Microimaging Gmbh | Vorrichtung und Verfahren zum räumlich hochauflösenden Abbilden einer Struktur einer Probe |
| US9134521B2 (en) * | 2008-07-30 | 2015-09-15 | The Regents Of The University Of California | Multidirectional selective plane illumination microscopy |
| DE102009044983A1 (de) | 2009-09-24 | 2011-03-31 | Carl Zeiss Microimaging Gmbh | Mikroskop |
| DE102009044984A1 (de) | 2009-09-24 | 2011-03-31 | Carl Zeiss Microimaging Gmbh | Mikroskop |
| WO2011059833A2 (en) | 2009-10-29 | 2011-05-19 | California Institute Of Technology | Dual-mode raster point scanning/light sheet illumination microscope |
| JP4894907B2 (ja) | 2009-11-17 | 2012-03-14 | カシオ計算機株式会社 | 撮像装置、撮像処理方法及びプログラム |
| US8711211B2 (en) * | 2010-06-14 | 2014-04-29 | Howard Hughes Medical Institute | Bessel beam plane illumination microscope |
| US10051240B2 (en) | 2010-06-14 | 2018-08-14 | Howard Hughes Medical Institute | Structured plane illumination microscopy |
| WO2012027542A2 (en) | 2010-08-25 | 2012-03-01 | California Institute Of Technology | Simultaneous orthogonal light sheet microscopy and computed optical tomography |
| DE102010060121C5 (de) | 2010-10-22 | 2023-09-28 | Leica Microsystems Cms Gmbh | SPIM-Mikroskop mit sequenziellem Lightsheet |
| US10908403B2 (en) | 2011-02-14 | 2021-02-02 | European Molecular Biology Laboratory (Embl) | Light-pad microscope for high-resolution 3D fluorescence imaging and 2D fluctuation spectroscopy |
| WO2012122027A2 (en) * | 2011-03-04 | 2012-09-13 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Optomechanical module for converting a microscope to provide selective plane illumination microscopy |
| JP6037623B2 (ja) | 2012-02-24 | 2016-12-07 | オリンパス株式会社 | レーザ走査型共焦点顕微鏡、及び、レーザ走査型共焦点顕微鏡の光学系のアライメント調整方法 |
| ES2898616T3 (es) | 2012-04-03 | 2022-03-08 | Univ Court Univ St Andrews | Imágenes de alta resolución de volúmenes extendidos |
| DE102012013163B4 (de) | 2012-07-02 | 2022-08-25 | Carl Zeiss Microscopy Gmbh | Mikroskop und Verfahren zur Lichtscheibenmikroskopie |
-
2014
- 2014-10-08 US US14/509,331 patent/US10539772B2/en active Active
- 2014-10-09 JP JP2016521691A patent/JP6364074B2/ja active Active
- 2014-10-09 EP EP14852724.5A patent/EP3055728B1/en active Active
- 2014-10-09 WO PCT/US2014/059822 patent/WO2015054450A1/en not_active Ceased
-
2018
- 2018-06-29 JP JP2018124403A patent/JP6625696B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2015054450A1 (en) | 2015-04-16 |
| JP2018185524A (ja) | 2018-11-22 |
| EP3055728B1 (en) | 2023-11-15 |
| JP2016538584A (ja) | 2016-12-08 |
| EP3055728A4 (en) | 2017-06-07 |
| EP3055728A1 (en) | 2016-08-17 |
| US20150098126A1 (en) | 2015-04-09 |
| US10539772B2 (en) | 2020-01-21 |
| JP6625696B2 (ja) | 2019-12-25 |
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