JP6339998B2 - 幹細胞よりナチュラルキラー細胞を発生させる方法 - Google Patents

幹細胞よりナチュラルキラー細胞を発生させる方法 Download PDF

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JP6339998B2
JP6339998B2 JP2015509079A JP2015509079A JP6339998B2 JP 6339998 B2 JP6339998 B2 JP 6339998B2 JP 2015509079 A JP2015509079 A JP 2015509079A JP 2015509079 A JP2015509079 A JP 2015509079A JP 6339998 B2 JP6339998 B2 JP 6339998B2
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ダン エス. カウフマン,
ダン エス. カウフマン,
デイビッド エー. クノール,
デイビッド エー. クノール,
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JP2015509079A 2012-04-24 2013-04-23 幹細胞よりナチュラルキラー細胞を発生させる方法 Active JP6339998B2 (ja)

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US201261637592P 2012-04-24 2012-04-24
US61/637,592 2012-04-24
PCT/US2013/037782 WO2013163171A1 (en) 2012-04-24 2013-04-23 Method for developing natural killer cells from stem cells

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JP2019091121A Pending JP2019122415A (ja) 2012-04-24 2019-05-14 幹細胞よりナチュラルキラー細胞を発生させる方法

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Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013163171A1 (en) * 2012-04-24 2013-10-31 Kaufman Dan S Method for developing natural killer cells from stem cells
WO2014165707A2 (en) 2013-04-03 2014-10-09 Memorial Sloan-Kettering Cancer Center Effective generation of tumor-targeted t-cells derived from pluripotent stem cells
WO2016123100A1 (en) * 2015-01-26 2016-08-04 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
WO2016143826A1 (ja) * 2015-03-09 2016-09-15 学校法人慶應義塾 多能性幹細胞を所望の細胞型へ分化する方法
WO2016187820A1 (zh) * 2015-05-26 2016-12-01 中国科学院广州生物医药与健康研究院 一种人原代细胞培养基及其应用
PL412787A1 (pl) * 2015-06-22 2017-01-02 Magdalena Król Oparty na makrofagach celowany system dostarczania związków związanych z ferrytyną
US10736963B2 (en) * 2015-07-24 2020-08-11 Innate Pharma Methods for detecting tissue infiltrating NK cells
SG11201801770VA (en) * 2015-09-08 2018-04-27 Sumitomo Dainippon Pharma Co Ltd Method for producing retinal pigment epithelial cells
SG11201803145RA (en) 2015-11-04 2018-05-30 Fate Therapeutics Inc Methods and compositions for inducing hematopoietic cell differentiation
KR20250141836A (ko) 2015-11-04 2025-09-29 페이트 세러퓨틱스, 인코포레이티드 만능 세포의 유전자 조작
CN105296428A (zh) * 2015-12-03 2016-02-03 广州医科大学附属第三医院 一种提高诱导多能干细胞的体外分化造血效率的方法
EP3827838B1 (en) 2015-12-16 2023-06-07 The Walter and Eliza Hall Institute of Medical Research Inhibition of cytokine-induced sh2 protein in nk cells
US20190161726A1 (en) * 2016-07-29 2019-05-30 Daikin Industries, Ltd. Differentiated cell production method and culture bag used therefor
US20190194621A1 (en) * 2016-09-08 2019-06-27 Kyowa Hakko Bio Co., Ltd. Medium for culturing stem cells, method for culturing stem cells, growth promoter for stem cells, and cell composition and method for producing same
EP3580332B1 (en) * 2017-02-07 2026-04-15 Agency for Science, Technology And Research Methods and kits for generating mimetic innate immune cells from pluripotent stem cells
IL309689B1 (en) * 2017-02-28 2026-02-01 Univ Central Florida Res Found Inc PM21 particles to enhance NK cell migration to the bone marrow
WO2018164240A1 (ja) 2017-03-08 2018-09-13 大日本住友製薬株式会社 網膜色素上皮細胞の製造方法
AU2018240416B2 (en) * 2017-03-21 2024-07-11 Centre National De La Recherche Scientifique Methods of improving hematopoietic grafts
KR102167548B1 (ko) * 2017-09-21 2020-10-19 한국생명공학연구원 자연살해세포의 제조방법 및 그의 용도
MX2020005907A (es) * 2017-12-08 2020-10-19 Juno Therapeutics Inc Formulacion de medio libre de suero para cultivar celulas y metodos de uso de la misma.
IL276365B2 (en) 2018-02-01 2023-10-01 Nkmax Co Ltd A method for the production of natural killer cells and a preparation for cancer treatment
EP3755347B1 (en) * 2018-02-21 2025-06-25 Board of Regents, The University of Texas System Universal antigen presenting cells and uses thereof
US12473336B2 (en) 2018-02-21 2025-11-18 Board Of Regents, The University Of Texas System Methods for activation and expansion of natural killer cells and uses thereof
US12516099B2 (en) 2018-08-09 2026-01-06 Juno Therapeutics, Inc. Processes for generating engineered cells and compositions thereof
CA3109804A1 (en) 2018-08-16 2020-02-20 The Regents Of The University Of California Chemically and photochemically initiated cell membrane blebbing to induce cell vesicle production, modifications thereof, and uses thereof
HRP20240821T1 (hr) 2018-08-27 2024-09-27 Affimed Gmbh Stanice nk čuvane zamrzavanjem, koje su prethodno ubačene uz protutijelni konstrukt
AU2019336221B2 (en) 2018-09-07 2025-05-15 Wisconsin Alumni Research Foundation Generation of hematopoietic progenitor cells from human pluripotent stem cells
WO2020180744A1 (en) * 2019-03-01 2020-09-10 The Regents Of The University Of California Natural killer cell induced cellular vesicles for cancer therapy
KR102298639B1 (ko) * 2019-03-28 2021-09-07 한국생명공학연구원 면역세포의 제조방법 및 그의 용도
RU2731293C1 (ru) 2019-04-12 2020-09-01 Игорь Петрович Белецкий Способ получения генно-модифицированных линий клеток натуральных киллеров с нокаутированным геном PD-1 и повышенной экспрессией белков семейства Фактора Некроза Опухолей для иммунотерапии онкологических заболеваний
WO2020220016A1 (en) * 2019-04-26 2020-10-29 H. Lee Moffitt Cancer Center And Research Institute, Inc. Artificial antigen presenting cells comprising ligands for nkg2d for expanding immune cells for immunotherapy
EP4060024A4 (en) * 2019-11-13 2023-12-06 Karis Bio Inc. METHOD FOR ISOLATING PURE CULTURE OF VASCULAR ENDOTHELIAL CELLS, MEDIUM FOR MAINTAINING THE CHARACTERISTICS OF VASCULAR ENDOTHELIAL CELLS, AND CULTURE METHOD COMPRISING SAME
CN111235105B (zh) * 2020-03-06 2021-08-10 安徽中盛溯源生物科技有限公司 一种从人多能干细胞分化为自然杀伤细胞的方法及应用
WO2021183776A1 (en) * 2020-03-11 2021-09-16 Research Institute At Nationwide Children's Hospital Nk cells and uses thereof for treatment of microbial infections
US11547726B2 (en) 2020-03-26 2023-01-10 Honed Life Sciences, Llc Enhancement of production of NK cells from stem cells
US20230257705A1 (en) 2020-06-17 2023-08-17 Kyoto University Chimeric antigen receptor-expressing immunocompetent cells
CN116096858A (zh) * 2020-07-27 2023-05-09 加拿大干细胞技术公司 用于分化造血细胞的系统和方法
US11459372B2 (en) 2020-11-30 2022-10-04 Crispr Therapeutics Ag Gene-edited natural killer cells
CN112608895B (zh) * 2020-12-18 2024-02-27 深圳市济因生物科技有限公司 一种人多能干细胞分化的自然杀伤细胞及其制备方法与应用
WO2022144632A1 (en) * 2020-12-30 2022-07-07 Crispr Therapeutics Ag Compositions and methods for differentiating stem cells into nk cells
EP4326852A4 (en) * 2021-04-22 2025-04-23 Artec Biotech, Inc. Method for producing hematopoietic cells from stem cells using vascular organoids
WO2022269393A1 (en) 2021-06-23 2022-12-29 Crispr Therapeutics Ag Engineered cells with improved protection from natural killer cell killing
CN115261318B (zh) * 2021-09-29 2024-05-28 苏州艾凯利元生物科技有限公司 产生自然杀伤细胞的方法
US20250000974A1 (en) * 2021-10-27 2025-01-02 The Regents Of The University Of California Combination of human natural killer cells and macrophages for cancer therapy
CN113957048A (zh) * 2021-10-29 2022-01-21 深圳市默赛尔生物医学科技发展有限公司 一种利用脐带血单个核细胞生产自然杀伤细胞的方法
MX2024007261A (es) 2021-12-17 2024-08-06 Umoja Biopharma Inc Celula linfoide innata citotoxica y usos de la misma.
TW202346574A (zh) * 2022-01-31 2023-12-01 日商希里歐斯股份有限公司 自然殺手細胞之製造方法
CN114507641B (zh) * 2022-04-20 2022-07-12 山东兴瑞生物科技有限公司 一种由人胚胎干细胞诱导分化为nk细胞的方法
WO2023233342A2 (en) 2022-06-01 2023-12-07 Crispr Therapeutics Ag Gene-edited natural killer cells
KR20250022772A (ko) * 2022-06-09 2025-02-17 우모자 바이오파마 인코포레이티드 Nk 세포 분화를 위한 조성물 및 방법
CN115058387B (zh) * 2022-06-11 2023-12-01 重庆医科大学 一种人胚胎干细胞与前列腺癌细胞共培养制备抗前列腺癌药物的方法
AU2023314782A1 (en) * 2022-07-27 2025-01-30 Umoja Biopharma, Inc. Differentiation of stem cells in suspension culture
WO2024101863A1 (ko) * 2022-11-07 2024-05-16 마루테라퓨틱스 주식회사 줄기세포 기원 및 발현 마커에 따른 nk 세포 분화 효율 증진 방법
WO2024101862A1 (ko) * 2022-11-07 2024-05-16 마루테라퓨틱스 주식회사 Nk 세포의 분화 효율 및 기능성 증가를 위한 배양 방법
EP4524241A4 (en) * 2022-11-10 2025-10-08 Sungkwang Medical Found MEDIUM COMPOSITION FOR INDUCING DIFFERENTIATION FROM PLURIPOTENT STEM CELLS INTO NATURAL KILLER CELLS AND DIFFERENTIATION METHOD USING SAME
AU2024263914A1 (en) * 2023-04-27 2025-12-04 Takeda Pharmaceutical Company Limited Method for expanding cd56+/cd3- cells

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0018737B1 (en) 1979-04-11 1983-11-09 Mitsui Petrochemical Industries, Ltd. Process for producing spherical carrier particles for olefin polymerisation catalyst, catalyst comprising such carrier particles, and use of such catalyst in olefin polymerisation
EP0187371B1 (en) 1984-12-27 1991-06-19 Asahi Kasei Kogyo Kabushiki Kaisha Substituted isoquinolinesulfonyl compounds
US6355476B1 (en) 1988-11-07 2002-03-12 Advanced Research And Technologyinc Nucleic acid encoding MIP-1α Lymphokine
CA2214649C (en) 1995-03-08 2007-06-12 Zeling Cai Antigen presenting system and methods for activation of t-cells
ES2278390T3 (es) 1996-05-23 2007-08-01 The Scripps Research Institute Sistema de presentacion de antigenos de clase ii de cmh y procedimientos de activacion de linfocitos t cd4+.
CA2277278A1 (en) 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
US6790662B1 (en) 1999-03-12 2004-09-14 Ortho-Mcneil Pharmaceutical, Inc. Method of isolating CD8+ cells, and related hybridoma cells antibodies and polypeptides
WO2000057913A1 (en) 1999-03-25 2000-10-05 Welfide Corporation Preventives/remedies for interstitial pneumonia and pulmonary fibrosis
WO2000078351A1 (en) 1999-06-18 2000-12-28 Mitsubishi Pharma Corporation Osteogenesis promoters
US20040071671A1 (en) 2001-02-20 2004-04-15 Leturcq Didier J. Cell therapy method for the treatment of tumors
MXPA04007196A (es) 2002-01-23 2005-06-08 Bayer Pharmaceuticals Corp Inhibidores de rho-quinasa.
US6664266B2 (en) 2002-03-14 2003-12-16 Children's Medical Center Corporation Axon regeneration with PKC inhibitiors
GB0206860D0 (en) 2002-03-22 2002-05-01 Glaxo Group Ltd Compounds
JP2005527209A (ja) 2002-04-22 2005-09-15 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 疼痛管理のためのプロテインキナーゼCγのペプチドインヒビター
ATE549395T1 (de) 2004-09-08 2012-03-15 Wisconsin Alumni Res Found Medium und kultur von embryonalen stammzellen
CA2643337C (en) 2006-03-01 2016-01-12 Janssen Pharmaceutica N.V. Cancer treatment combining lymphodepleting agent with ctls and cytokines
JP5543207B2 (ja) 2006-10-04 2014-07-09 ジヤンセン・フアーマシユーチカ・ナームローゼ・フエンノートシヤツプ 不活性化された人工抗原提示細胞の調製および細胞治療におけるそれらの使用
US8513012B2 (en) 2008-05-02 2013-08-20 Cellular Dynamics International, Inc. Method for production of mast cells from stem cells
KR101720961B1 (ko) 2009-02-27 2017-03-29 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 다능성 세포의 분화
CA3080368A1 (en) 2009-12-04 2011-06-09 Stem Cell & Regenerative Medicine International, Inc. Method of generating natural killer cells and dendritic cells from human embryonic stem cell-derived hemangioblasts
WO2013163171A1 (en) * 2012-04-24 2013-10-31 Kaufman Dan S Method for developing natural killer cells from stem cells

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