JP6282467B2 - タンパク質を生産するための方法 - Google Patents
タンパク質を生産するための方法 Download PDFInfo
- Publication number
- JP6282467B2 JP6282467B2 JP2013532180A JP2013532180A JP6282467B2 JP 6282467 B2 JP6282467 B2 JP 6282467B2 JP 2013532180 A JP2013532180 A JP 2013532180A JP 2013532180 A JP2013532180 A JP 2013532180A JP 6282467 B2 JP6282467 B2 JP 6282467B2
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- JP
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- Prior art keywords
- culture
- protein
- cells
- suspension
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 102000004169 proteins and genes Human genes 0.000 title claims description 38
- 108090000623 proteins and genes Proteins 0.000 title claims description 38
- 238000004519 manufacturing process Methods 0.000 title claims description 14
- 238000000034 method Methods 0.000 claims description 56
- 230000002439 hemostatic effect Effects 0.000 claims description 26
- 102100022641 Coagulation factor IX Human genes 0.000 claims description 23
- 108010076282 Factor IX Proteins 0.000 claims description 23
- 238000004113 cell culture Methods 0.000 claims description 20
- 239000000725 suspension Substances 0.000 claims description 20
- 229960004222 factor ix Drugs 0.000 claims description 19
- 230000010412 perfusion Effects 0.000 claims description 13
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 210000004027 cell Anatomy 0.000 description 49
- 102100023804 Coagulation factor VII Human genes 0.000 description 10
- 108010023321 Factor VII Proteins 0.000 description 9
- 108010054218 Factor VIII Proteins 0.000 description 9
- 102000001690 Factor VIII Human genes 0.000 description 9
- 229940012413 factor vii Drugs 0.000 description 8
- 229960000301 factor viii Drugs 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 230000035899 viability Effects 0.000 description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 239000002609 medium Substances 0.000 description 5
- 238000011084 recovery Methods 0.000 description 4
- 239000006143 cell culture medium Substances 0.000 description 3
- 230000003833 cell viability Effects 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000011143 downstream manufacturing Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 2
- 239000004695 Polyether sulfone Substances 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 238000011437 continuous method Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229920006393 polyether sulfone Polymers 0.000 description 2
- 230000014616 translation Effects 0.000 description 2
- 241000699800 Cricetinae Species 0.000 description 1
- 241000699802 Cricetulus griseus Species 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- ABSPRNADVQNDOU-UHFFFAOYSA-N Menaquinone 1 Natural products C1=CC=C2C(=O)C(CC=C(C)C)=C(C)C(=O)C2=C1 ABSPRNADVQNDOU-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000008425 Protein deficiency Diseases 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- UHBYWPGGCSDKFX-UHFFFAOYSA-N carboxyglutamic acid Chemical compound OC(=O)C(N)CC(C(O)=O)C(O)=O UHBYWPGGCSDKFX-UHFFFAOYSA-N 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229960001031 glucose Drugs 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 229960002743 glutamine Drugs 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 description 1
- MBWXNTAXLNYFJB-NKFFZRIASA-N phylloquinone Chemical compound C1=CC=C2C(=O)C(C/C=C(C)/CCC[C@H](C)CCC[C@H](C)CCCC(C)C)=C(C)C(=O)C2=C1 MBWXNTAXLNYFJB-NKFFZRIASA-N 0.000 description 1
- 235000019175 phylloquinone Nutrition 0.000 description 1
- 239000011772 phylloquinone Substances 0.000 description 1
- 229960001898 phytomenadione Drugs 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6437—Coagulation factor VIIa (3.4.21.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/644—Coagulation factor IXa (3.4.21.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21022—Coagulation factor IXa (3.4.21.22)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10186545.9 | 2010-10-05 | ||
| EP10186545 | 2010-10-05 | ||
| US39271310P | 2010-10-13 | 2010-10-13 | |
| US61/392,713 | 2010-10-13 | ||
| PCT/EP2011/067372 WO2012045769A1 (en) | 2010-10-05 | 2011-10-05 | Process for protein production |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016153606A Division JP2016187360A (ja) | 2010-10-05 | 2016-08-04 | タンパク質を生産するための方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013538588A JP2013538588A (ja) | 2013-10-17 |
| JP2013538588A5 JP2013538588A5 (cg-RX-API-DMAC7.html) | 2014-11-20 |
| JP6282467B2 true JP6282467B2 (ja) | 2018-02-21 |
Family
ID=43735957
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013532180A Active JP6282467B2 (ja) | 2010-10-05 | 2011-10-05 | タンパク質を生産するための方法 |
| JP2016153606A Withdrawn JP2016187360A (ja) | 2010-10-05 | 2016-08-04 | タンパク質を生産するための方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016153606A Withdrawn JP2016187360A (ja) | 2010-10-05 | 2016-08-04 | タンパク質を生産するための方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US10138290B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP2957628A1 (cg-RX-API-DMAC7.html) |
| JP (2) | JP6282467B2 (cg-RX-API-DMAC7.html) |
| CN (2) | CN107190034A (cg-RX-API-DMAC7.html) |
| ES (1) | ES2556454T3 (cg-RX-API-DMAC7.html) |
| PL (1) | PL2625262T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2012045769A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015526094A (ja) * | 2012-08-20 | 2015-09-10 | テルモ ビーシーティー、インコーポレーテッド | 細胞成長チャンバーを循環する流体の成分を濃縮する方法 |
| US10421986B2 (en) | 2013-09-30 | 2019-09-24 | Janssen Vaccines & Prevention B.V. | Method for the clarification of high-density crude cell culture harvest |
| JP6530171B2 (ja) * | 2014-09-09 | 2019-06-12 | 旭化成メディカル株式会社 | 培養産生物の回収方法 |
| KR102539167B1 (ko) * | 2016-07-25 | 2023-06-02 | 리플리겐 코포레이션 | 교번 접선 유동식의 신속한 수확 |
| CN111263631A (zh) * | 2017-10-26 | 2020-06-09 | 瑞普利金公司 | 微型交替切向流灌注过滤器、处理容器及其使用方法 |
| TWI898645B (zh) | 2018-05-04 | 2025-09-21 | 美商健臻公司 | 灌注式細胞培養裝置 |
| CN108504562A (zh) * | 2018-06-21 | 2018-09-07 | 江苏澳创生物科技有限公司 | 一种发酵生产l-苏氨酸的系统及其应用 |
| JP7680418B2 (ja) * | 2019-07-16 | 2025-05-20 | イノベント バイオロジクス（スーチョウ）カンパニー，リミティド | 高密度連続接種の細胞培養方法及びその応用 |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2584443B2 (ja) | 1985-04-22 | 1997-02-26 | ジエネテイツクス・インスチチユ−ト・インコ−ポレ−テツド | 活性化▲ｉ▼▲ｘ▼因子の高収率産生 |
| DE3785102T2 (de) | 1986-01-03 | 1993-07-22 | Genetics Inst | Verfahren zur herstellung von faktor-viii:c-typ-proteinen. |
| CA1331157C (en) | 1987-04-06 | 1994-08-02 | Randal J. Kaufman | Method for producing factor viii:c-type proteins |
| US4956080A (en) * | 1987-08-03 | 1990-09-11 | Microlift Systems, Incorporated | High pressure oxygen-saturated water treatment apparatus |
| JPH066054B2 (ja) | 1987-10-15 | 1994-01-26 | 帝人株式会社 | 動物細胞の培養方法 |
| JPH084495B2 (ja) | 1988-08-19 | 1996-01-24 | 帝人株式会社 | 接着性動物細胞の培養方法 |
| WO1990002175A1 (en) | 1988-08-16 | 1990-03-08 | Novo Nordisk A/S | A method of producing polypeptides by culturing eukaryotic cells in the presence of protease inhibitors |
| US6268487B1 (en) | 1996-05-13 | 2001-07-31 | Genzyme Transgenics Corporation | Purification of biologically active peptides from milk |
| US5851800A (en) | 1996-05-14 | 1998-12-22 | Pharmacia & Upjohn Ab | Process for producing a protein |
| AT409379B (de) * | 1999-06-02 | 2002-07-25 | Baxter Ag | Medium zur protein- und serumfreien kultivierung von zellen |
| US6544424B1 (en) * | 1999-12-03 | 2003-04-08 | Refined Technology Company | Fluid filtration system |
| JP4399158B2 (ja) | 2000-10-20 | 2010-01-13 | バイオニアー エイ／エス | 乳酸菌における異種遺伝子産物の製造のための改良発酵法 |
| JP2005512524A (ja) | 2001-11-02 | 2005-05-12 | ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト | ヒト凝固第ｖｉｉ因子ポリペプチド |
| US6960657B2 (en) | 2001-11-02 | 2005-11-01 | Novo Nordisk Healthcare A/G | Human coagulation factor VII polypeptides |
| ATE503498T1 (de) | 2002-06-21 | 2011-04-15 | Novo Nordisk Healthcare Ag | Pegylierte glykoformen von faktor vii |
| US7351688B2 (en) | 2003-02-05 | 2008-04-01 | The Research Foundation Of State University Of New York | Compositions and methods for less immunogenic protein formulations |
| KR20060015568A (ko) | 2003-05-01 | 2006-02-17 | 디에스엠 아이피 어셋츠 비.브이. | 현탁된 동물 세포의 관류 배양에 의한 생물학적 물질의제조방법 |
| EA008670B1 (ru) | 2003-05-09 | 2007-06-29 | Круселл Холланд Б.В. | Культуры e1-иммортализованных клеток и способы их культивирования с целью повышения выхода их продукции |
| EP1673452B1 (en) | 2003-10-10 | 2015-12-23 | Novo Nordisk Health Care AG | Method for large-scale production of a polypeptide in eukaryote cells |
| EP1720972B1 (en) * | 2004-03-05 | 2014-01-08 | DSM IP Assets B.V. | Process for cell culturing by continuous perfusion and alternating tangential flow |
| JP2008509688A (ja) | 2004-08-17 | 2008-04-03 | ツェー・エス・エル・ベーリング・ゲー・エム・ベー・ハー | 改変ビタミンｋ依存性ポリペプチド |
| EP1707634A1 (en) | 2005-03-29 | 2006-10-04 | Octapharma AG | Method for isolation of recombinantly produced proteins |
| CN101473029A (zh) | 2006-04-21 | 2009-07-01 | 拜尔健康护理有限责任公司 | 抗凋亡基因在用于灌注培养的哺乳动物细胞中的应用 |
| CN101490239A (zh) * | 2006-07-14 | 2009-07-22 | 帝斯曼知识产权资产管理有限公司 | 改进的细胞培养方法 |
| PL2343362T5 (pl) * | 2006-07-14 | 2024-12-02 | Patheon Holdings I B.V. | Ulepszony proces hodowli komórek |
| EP1988101A1 (en) | 2007-05-04 | 2008-11-05 | Novo Nordisk A/S | Improvement of factor VIII polypeptide titers in cell cultures |
| EP2014760A1 (en) | 2007-06-13 | 2009-01-14 | CMC Biopharmaceuticals A/S | A method for producing a biopolymer (e.g. polypeptide) in a continuous fermentation process |
| JP2009045019A (ja) | 2007-08-21 | 2009-03-05 | Toray Ind Inc | タンパク質の製造方法 |
| US20110137011A1 (en) | 2008-04-21 | 2011-06-09 | Novo Nordisk A/S | Hyperglycosylated human coagulation factor ix |
| WO2010003759A2 (en) | 2008-06-17 | 2010-01-14 | Dsm Ip Assets B.V. | Cell culturing method |
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- 2011-10-05 EP EP15174742.5A patent/EP2957628A1/en not_active Withdrawn
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|---|---|
| JP2013538588A (ja) | 2013-10-17 |
| PL2625262T3 (pl) | 2016-05-31 |
| ES2556454T3 (es) | 2016-01-18 |
| US10138290B2 (en) | 2018-11-27 |
| WO2012045769A1 (en) | 2012-04-12 |
| EP2957628A1 (en) | 2015-12-23 |
| JP2016187360A (ja) | 2016-11-04 |
| EP2625262A1 (en) | 2013-08-14 |
| EP2625262B1 (en) | 2015-09-23 |
| CN107190034A (zh) | 2017-09-22 |
| CN103154233A (zh) | 2013-06-12 |
| US20130244283A1 (en) | 2013-09-19 |
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