CN107190034A - 生产蛋白质的方法 - Google Patents
生产蛋白质的方法 Download PDFInfo
- Publication number
- CN107190034A CN107190034A CN201710499437.8A CN201710499437A CN107190034A CN 107190034 A CN107190034 A CN 107190034A CN 201710499437 A CN201710499437 A CN 201710499437A CN 107190034 A CN107190034 A CN 107190034A
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- cell
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- 230000010412 perfusion Effects 0.000 description 11
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- UHBYWPGGCSDKFX-UHFFFAOYSA-N carboxyglutamic acid Chemical compound OC(=O)C(N)CC(C(O)=O)C(O)=O UHBYWPGGCSDKFX-UHFFFAOYSA-N 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
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- 102000039446 nucleic acids Human genes 0.000 description 1
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- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 description 1
- MBWXNTAXLNYFJB-NKFFZRIASA-N phylloquinone Chemical compound C1=CC=C2C(=O)C(C/C=C(C)/CCC[C@H](C)CCC[C@H](C)CCCC(C)C)=C(C)C(=O)C2=C1 MBWXNTAXLNYFJB-NKFFZRIASA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6437—Coagulation factor VIIa (3.4.21.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/644—Coagulation factor IXa (3.4.21.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21022—Coagulation factor IXa (3.4.21.22)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10186545.9 | 2010-10-05 | ||
| EP10186545 | 2010-10-05 | ||
| US39271310P | 2010-10-13 | 2010-10-13 | |
| US61/392713 | 2010-10-13 | ||
| CN2011800482244A CN103154233A (zh) | 2010-10-05 | 2011-10-05 | 生产蛋白质的方法 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011800482244A Division CN103154233A (zh) | 2010-10-05 | 2011-10-05 | 生产蛋白质的方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107190034A true CN107190034A (zh) | 2017-09-22 |
Family
ID=43735957
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011800482244A Pending CN103154233A (zh) | 2010-10-05 | 2011-10-05 | 生产蛋白质的方法 |
| CN201710499437.8A Pending CN107190034A (zh) | 2010-10-05 | 2011-10-05 | 生产蛋白质的方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011800482244A Pending CN103154233A (zh) | 2010-10-05 | 2011-10-05 | 生产蛋白质的方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US10138290B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP2625262B1 (cg-RX-API-DMAC7.html) |
| JP (2) | JP6282467B2 (cg-RX-API-DMAC7.html) |
| CN (2) | CN103154233A (cg-RX-API-DMAC7.html) |
| ES (1) | ES2556454T3 (cg-RX-API-DMAC7.html) |
| PL (1) | PL2625262T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2012045769A1 (cg-RX-API-DMAC7.html) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111263631A (zh) * | 2017-10-26 | 2020-06-09 | 瑞普利金公司 | 微型交替切向流灌注过滤器、处理容器及其使用方法 |
| WO2021008571A1 (zh) * | 2019-07-16 | 2021-01-21 | 信达生物制药(苏州)有限公司 | 高密度连续接种的细胞培养方法及其应用 |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015526094A (ja) * | 2012-08-20 | 2015-09-10 | テルモ ビーシーティー、インコーポレーテッド | 細胞成長チャンバーを循環する流体の成分を濃縮する方法 |
| BR112016006063A2 (pt) * | 2013-09-30 | 2017-08-01 | Crucell Holland Bv | método para clarificação de uma colheita de cultura celular em bruto |
| JP6530171B2 (ja) * | 2014-09-09 | 2019-06-12 | 旭化成メディカル株式会社 | 培養産生物の回収方法 |
| AU2017301691B2 (en) * | 2016-07-25 | 2022-07-21 | Repligen Corporation | Alternating tangential flow rapid harvesting |
| TWI846694B (zh) * | 2018-05-04 | 2024-07-01 | 美商健臻公司 | 具有過濾系統的灌注式生物反應器 |
| CN108504562A (zh) * | 2018-06-21 | 2018-09-07 | 江苏澳创生物科技有限公司 | 一种发酵生产l-苏氨酸的系统及其应用 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101490239A (zh) * | 2006-07-14 | 2009-07-22 | 帝斯曼知识产权资产管理有限公司 | 改进的细胞培养方法 |
| WO2009130198A2 (en) * | 2008-04-21 | 2009-10-29 | Novo Nordisk A/S | Hyperglycosylated human coagulation factor ix |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4770999A (en) | 1985-04-22 | 1988-09-13 | Genetics Institute, Inc. | High yield production of active Factor IX |
| EP0253870B1 (en) | 1986-01-03 | 1993-03-31 | Genetics Institute, Inc. | Method for producing factor viii:c-type proteins |
| CA1331157C (en) | 1987-04-06 | 1994-08-02 | Randal J. Kaufman | Method for producing factor viii:c-type proteins |
| US4956080A (en) * | 1987-08-03 | 1990-09-11 | Microlift Systems, Incorporated | High pressure oxygen-saturated water treatment apparatus |
| JPH066054B2 (ja) | 1987-10-15 | 1994-01-26 | 帝人株式会社 | 動物細胞の培養方法 |
| JPH084495B2 (ja) | 1988-08-19 | 1996-01-24 | 帝人株式会社 | 接着性動物細胞の培養方法 |
| WO1990002175A1 (en) | 1988-08-16 | 1990-03-08 | Novo Nordisk A/S | A method of producing polypeptides by culturing eukaryotic cells in the presence of protease inhibitors |
| US6268487B1 (en) | 1996-05-13 | 2001-07-31 | Genzyme Transgenics Corporation | Purification of biologically active peptides from milk |
| US5851800A (en) | 1996-05-14 | 1998-12-22 | Pharmacia & Upjohn Ab | Process for producing a protein |
| AT409379B (de) * | 1999-06-02 | 2002-07-25 | Baxter Ag | Medium zur protein- und serumfreien kultivierung von zellen |
| US6544424B1 (en) | 1999-12-03 | 2003-04-08 | Refined Technology Company | Fluid filtration system |
| US7358067B2 (en) | 2000-10-20 | 2008-04-15 | Bioneer A/S | Fermentation method for production of heterologous gene products in lactic acid bacteria |
| ES2490590T3 (es) | 2001-11-02 | 2014-09-04 | Novo Nordisk Health Care Ag | Polipéptidos de factor VII de coagulación humana |
| US6960657B2 (en) | 2001-11-02 | 2005-11-01 | Novo Nordisk Healthcare A/G | Human coagulation factor VII polypeptides |
| DK1517710T3 (da) | 2002-06-21 | 2011-07-18 | Novo Nordisk Healthcare Ag | Pegylerede faktor VII-glycoformer |
| US7351688B2 (en) | 2003-02-05 | 2008-04-01 | The Research Foundation Of State University Of New York | Compositions and methods for less immunogenic protein formulations |
| CN100432217C (zh) | 2003-05-01 | 2008-11-12 | 帝斯曼知识产权资产管理有限公司 | 通过对悬浮的动物细胞进行灌流式培养来生产生物物质的方法 |
| CN101328473B (zh) | 2003-05-09 | 2012-05-02 | 克鲁塞尔荷兰公司 | E1-永生化的细胞培养物及培养所述细胞以提高从中获取的产物产量的方法 |
| ES2565077T3 (es) | 2003-10-10 | 2016-03-31 | Novo Nordisk Health Care Ag | Método para producción en gran escala de un polipéptido en células eucariotas |
| CN1930281A (zh) * | 2004-03-05 | 2007-03-14 | 帝斯曼知识产权资产管理有限公司 | 用于通过连续灌注和交互切向流来培养细胞的方法 |
| US8828939B2 (en) | 2004-08-17 | 2014-09-09 | Csl Behring Gmbh | Modified vitamin K dependent polypeptides |
| EP1707634A1 (en) | 2005-03-29 | 2006-10-04 | Octapharma AG | Method for isolation of recombinantly produced proteins |
| CA2649912A1 (en) | 2006-04-21 | 2007-11-01 | Bayer Healthcare Llc | Application of anti-apoptotic genes in mammalian cells for perfusion culture |
| MX2009000522A (es) * | 2006-07-14 | 2009-01-27 | Dsm Ip Assets Bv | Proceso mejorado para el cultivo de celulas. |
| EP1988101A1 (en) | 2007-05-04 | 2008-11-05 | Novo Nordisk A/S | Improvement of factor VIII polypeptide titers in cell cultures |
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- 2011-10-05 US US13/823,847 patent/US10138290B2/en active Active
- 2011-10-05 CN CN2011800482244A patent/CN103154233A/zh active Pending
- 2011-10-05 CN CN201710499437.8A patent/CN107190034A/zh active Pending
- 2011-10-05 WO PCT/EP2011/067372 patent/WO2012045769A1/en not_active Ceased
- 2011-10-05 EP EP11764561.4A patent/EP2625262B1/en not_active Revoked
- 2011-10-05 JP JP2013532180A patent/JP6282467B2/ja active Active
- 2011-10-05 EP EP15174742.5A patent/EP2957628A1/en not_active Withdrawn
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2016
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111263631A (zh) * | 2017-10-26 | 2020-06-09 | 瑞普利金公司 | 微型交替切向流灌注过滤器、处理容器及其使用方法 |
| US11946033B2 (en) | 2017-10-26 | 2024-04-02 | Repligen Corporation | Micro alternating tangential flow perfusion filter, micro bioreactor, and methods of use thereof |
| WO2021008571A1 (zh) * | 2019-07-16 | 2021-01-21 | 信达生物制药(苏州)有限公司 | 高密度连续接种的细胞培养方法及其应用 |
| CN114127260A (zh) * | 2019-07-16 | 2022-03-01 | 信达生物制药(苏州)有限公司 | 高密度连续接种的细胞培养方法及其应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| US20130244283A1 (en) | 2013-09-19 |
| JP6282467B2 (ja) | 2018-02-21 |
| EP2957628A1 (en) | 2015-12-23 |
| JP2013538588A (ja) | 2013-10-17 |
| EP2625262B1 (en) | 2015-09-23 |
| CN103154233A (zh) | 2013-06-12 |
| US10138290B2 (en) | 2018-11-27 |
| EP2625262A1 (en) | 2013-08-14 |
| WO2012045769A1 (en) | 2012-04-12 |
| PL2625262T3 (pl) | 2016-05-31 |
| ES2556454T3 (es) | 2016-01-18 |
| JP2016187360A (ja) | 2016-11-04 |
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