JP6197039B2 - ハイスループット多重検出用システム、装置及び方法 - Google Patents

ハイスループット多重検出用システム、装置及び方法 Download PDF

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JP6197039B2
JP6197039B2 JP2015528698A JP2015528698A JP6197039B2 JP 6197039 B2 JP6197039 B2 JP 6197039B2 JP 2015528698 A JP2015528698 A JP 2015528698A JP 2015528698 A JP2015528698 A JP 2015528698A JP 6197039 B2 JP6197039 B2 JP 6197039B2
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cells
cell
separation
protein
capture agent
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ファン，ロン
ル，ヤオ
チェン，ジョナサン
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Yale University
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/70—Mechanisms involved in disease identification
    • G01N2800/7023—(Hyper)proliferation
    • G01N2800/7028—Cancer

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  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
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  • Molecular Biology (AREA)
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  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2015528698A 2012-08-24 2013-08-23 ハイスループット多重検出用システム、装置及び方法 Active JP6197039B2 (ja)

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US201261692895P 2012-08-24 2012-08-24
US61/692,895 2012-08-24
US201361779299P 2013-03-13 2013-03-13
US61/779,299 2013-03-13
PCT/US2013/056454 WO2014031997A1 (en) 2012-08-24 2013-08-23 System, device and method for high-throughput multi-plexed detection

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JP2019164396A Pending JP2020046423A (ja) 2012-08-24 2019-09-10 ハイスループット多重検出用システム、装置及び方法

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Families Citing this family (103)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009012340A2 (en) 2007-07-16 2009-01-22 California Institute Of Technology Microfluidic devices, methods and systems for detecting target molecules
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US11435358B2 (en) 2011-06-23 2022-09-06 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
EP2739587B1 (en) 2011-08-01 2020-05-27 Denovo Sciences Cell capture system
US10466160B2 (en) 2011-08-01 2019-11-05 Celsee Diagnostics, Inc. System and method for retrieving and analyzing particles
US9404864B2 (en) 2013-03-13 2016-08-02 Denovo Sciences, Inc. System for imaging captured cells
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
CN108267581A (zh) 2012-08-24 2018-07-10 耶鲁大学 用于高通量多重检测的系统、装置和方法
US9752181B2 (en) 2013-01-26 2017-09-05 Denovo Sciences, Inc. System and method for capturing and analyzing cells
US9707562B2 (en) 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
US10391490B2 (en) 2013-05-31 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
US9856535B2 (en) 2013-05-31 2018-01-02 Denovo Sciences, Inc. System for isolating cells
DE102013211850A1 (de) * 2013-06-21 2014-12-24 Gilupi Gmbh Schnelltest zum Nachweisen von Erregermaterial, insbesondere zur Unterstützung der Diagnose einer Sepsis, sowie Kit und Vorrichtung zur Durchführung eines Sepsistests
ES2711168T3 (es) 2013-08-28 2019-04-30 Becton Dickinson Co Análisis masivo en paralelo de células individuales
FR3019899B1 (fr) * 2014-04-09 2017-12-22 Bio-Rad Innovations Utilisation d'un colorant pour ameliorer la detection du signal dans un procede d'analyse
CN112782140A (zh) 2014-12-03 2021-05-11 伊索普莱西斯公司 细胞分泌特征的分析和筛选
CN107209934B (zh) * 2014-12-03 2021-03-30 文塔纳医疗系统公司 用于定量分析异质生物标志物分布的方法、系统和装置
EP3240885B1 (en) * 2014-12-31 2024-11-13 Fluidigm Canada Inc. Structured biological samples for analysis by mass cytometry
WO2016115537A2 (en) 2015-01-15 2016-07-21 Massachusetts Institute Of Technology Systems, methods, and apparatus for in vitro single-cell identification and recovery
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
US10712356B2 (en) 2015-04-21 2020-07-14 General Automation Lab Technologies Inc. Apparatus and method for picking biological sample
SG11201708579WA (en) * 2015-04-21 2017-11-29 Gen Automation Lab Tech Inc High resolution systems, kits, apparatus, and methods for high throughput microbiology applications
TWI737614B (zh) 2015-06-11 2021-09-01 博錸生技股份有限公司 已編碼微載體及其製造方法以及用以進行多工分析之包含該等已編碼微載體之套件
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
JP6599472B2 (ja) 2015-09-29 2019-10-30 東京応化工業株式会社 基板、構造体、構造体の製造方法、細胞の選別方法、細胞の製造方法、及び分泌物の産生方法
US10436776B2 (en) 2015-11-20 2019-10-08 Plexbio Co., Ltd. Methods and systems for selection of detection area
DK3384046T3 (da) * 2015-12-01 2021-07-12 Illumina Inc Digitalt mikrofluidisk system til enkeltcelleisolering og karakterisering af analytter
US20200398277A9 (en) * 2016-01-16 2020-12-24 Spin Bio, Llc. Tools and methods for isolation and analysis of individual components from a biological sample
US10019815B2 (en) 2016-03-17 2018-07-10 Plexbio Co., Ltd. Methods and systems for image differentiated multiplex assays
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP3472351B1 (de) 2016-06-15 2020-10-28 Ludwig-Maximilians-Universität München Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie
EP3510398B1 (en) 2016-09-12 2026-02-25 Isoplexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
US11796535B2 (en) 2016-09-16 2023-10-24 Plexbio Co., Ltd. Methods and systems for multiplex assays
CA3034924C (en) 2016-09-26 2025-11-18 Becton, Dickinson And Company MEASUREMENT OF PROTEIN EXPRESSION USING REAGENTS WITH BARCODED OLIGONUCLEOTIDE SEQUENCES
WO2018061733A1 (ja) * 2016-09-28 2018-04-05 富士フイルム株式会社 検査方法および検査チップ
JP2020524480A (ja) 2016-11-03 2020-08-20 セル マイクロシステムズ インクＣｅｌｌ Ｍｉｃｒｏｓｙｓｔｅｍｓ， Ｉｎｃ． 特定の数の細胞の自動収集
EP4036578A1 (en) * 2016-11-11 2022-08-03 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
CN110226084A (zh) 2016-11-22 2019-09-10 伊索普莱克西斯公司 用于细胞捕获的系统、装置和方法，以及其制造方法
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
EP3580354A4 (en) 2017-02-13 2021-03-31 Yale University HIGH THROUGHPUT SINGLE CELL POLYOMICS
WO2018179735A1 (ja) * 2017-03-28 2018-10-04 ソニー株式会社 細胞の生産能力を分析するためのデバイス、細胞の生産能力を分析するための装置、細胞の生産能力を分析する方法及び細胞の生産能力を分析するためのシステム
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
WO2019046307A1 (en) * 2017-08-29 2019-03-07 Celsee Diagnostics, Inc. SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS
WO2019088904A1 (en) * 2017-10-31 2019-05-09 Astrego Diagnostics Ab Microfluidic device for cell characterization
US12122988B2 (en) 2018-03-12 2024-10-22 Sony Corporation Cell evaluation device and cell evaluation system
CN110343655A (zh) * 2018-04-02 2019-10-18 中国科学院大连化学物理研究所 一种集成细胞捕获、三维基底培养及生物分析的芯片
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
CN110499251A (zh) * 2018-05-17 2019-11-26 中国科学院大连化学物理研究所 高通量多参数的单细胞源性细胞外囊泡分析芯片及应用
AU2019301750A1 (en) 2018-07-12 2021-01-28 Board Of Regents, The University Of Texas System Molecular neighborhood detection by oligonucleotides
JP7172344B2 (ja) * 2018-09-20 2022-11-16 東洋紡株式会社 分析方法および分析用装置
ES2992135T3 (es) 2018-10-01 2024-12-09 Becton Dickinson Co Determinar secuencias de transcripción 5’
GB2593091B (en) 2018-10-05 2023-12-20 Univ Texas Solid-phase N-terminal peptide capture and release
CN109521202A (zh) * 2018-10-31 2019-03-26 江苏师范大学 一种基于数字免疫分析技术的低丰度蛋白质绝对定量方法
JP7618548B2 (ja) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
WO2020117975A1 (en) * 2018-12-04 2020-06-11 IsoPlexis Corporation Systems, devices and methods for identification, selective ablation, and selection and collection of single cells
EP3894590B1 (en) * 2018-12-10 2025-10-22 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
CN109765356A (zh) * 2019-01-18 2019-05-17 江苏医联生物科技有限公司 一种蛋白质芯片荧光检测方法
EP4242322B1 (en) 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
CN109991423B (zh) * 2019-01-29 2020-06-26 厦门大学 高效单细胞捕获与快速单细胞分泌蛋白检测平台及检测方法
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
US10633693B1 (en) 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
EP3966307B1 (en) 2019-05-07 2024-11-20 Bio-Rad Laboratories, Inc. System and method for automated single cell processing
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
SG11202112898WA (en) 2019-06-14 2021-12-30 Bio Rad Laboratories System and method for automated single cell processing and analyses
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
CN114787348B (zh) 2019-09-30 2025-09-19 耶鲁大学 用于空间组学测序的确定性条形码
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用
CN112798785A (zh) * 2019-11-13 2021-05-14 中国科学院大连化学物理研究所 一种可实现细胞捕获与多指标生物分析的芯片与应用
EP4090764A1 (en) 2020-01-13 2022-11-23 Becton, Dickinson and Company Cell capture using du-containing oligonucleotides
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
CN115298322A (zh) * 2020-01-17 2022-11-04 贝克顿迪金森公司 用于单细胞分泌组学的方法和组合物
WO2021154230A1 (en) 2020-01-29 2021-08-05 Hewlett-Packard Development Company, L.P. Antibody fluid deposits
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US12064768B2 (en) 2020-02-21 2024-08-20 Northeastern University Single cell isolation and processing system with reversible well shape
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11504719B2 (en) 2020-03-12 2022-11-22 Bio-Rad Laboratories, Inc. System and method for receiving and delivering a fluid for sample processing
WO2021230799A1 (en) * 2020-05-14 2021-11-18 Camsund Daniel A microfluidic device having specifically designed detection chambers
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
CN113009145A (zh) * 2020-06-17 2021-06-22 山东大学 一种用于治疗细胞分泌蛋白检测的微流控芯片及制备方法
CN113030481A (zh) * 2020-06-17 2021-06-25 山东大学 一种单细胞外泌体静态检测试剂盒及检测分析方法
CN113008764B (zh) * 2020-06-17 2022-12-02 山东大学 一种单细胞功能评估试剂盒及评估方法
CN113009144B (zh) * 2020-06-17 2023-07-21 山东大学 一种基于微流控技术的抗体检测试剂盒及检测方法
CN113005022B (zh) * 2020-07-06 2024-04-26 山东大学 一种用于同一个单细胞分泌蛋白、细胞质mRNA和核内DNA的同步检测芯片及方法
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CA3186058A1 (en) * 2020-07-27 2022-02-03 Benjamin Marc Feder Fogelson Techniques for analyzing and detecting executional artifacts in microwell plates
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
WO2022125701A1 (en) * 2020-12-09 2022-06-16 Becton, Dickinson And Company Multiplexed single cell immunoassay
EP4263859A1 (en) * 2020-12-15 2023-10-25 Becton, Dickinson and Company Single cell secretome analysis
US12576400B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for incubating and analyzing a cell in a compartment of a fluidic device
JP2024502618A (ja) 2021-01-08 2024-01-22 セラノーム， インコーポレイテッド 生体試料を分析するためのデバイスおよび方法
WO2022178253A1 (en) 2021-02-19 2022-08-25 IsoPlexis Corporation Methods and devices for spatially resolved analysis of proteomic and genetic information
WO2022182832A1 (en) * 2021-02-24 2022-09-01 Board Of Regents, The University Of Texas System Methods for polypeptide processing and analysis
JP2024527371A (ja) * 2021-07-08 2024-07-24 オウロテック インコーポレイテッド 処置の有効性予測システム及び方法
CN114073996B (zh) * 2021-11-24 2023-04-14 中山大学 一种嵌套式微井阵列芯片及其制备方法
WO2023107539A1 (en) 2021-12-10 2023-06-15 Bio-Rad Laboratories, Inc. Compositions, methods, and systems for sample processing with morphology-adjustable functionalized particles
CN116930047A (zh) * 2023-08-11 2023-10-24 中翰盛泰生物技术股份有限公司 一种用于流式荧光检测前处理装置及其处理方法

Family Cites Families (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6943034B1 (en) 1991-11-22 2005-09-13 Affymetrix, Inc. Combinatorial strategies for polymer synthesis
EP0821726B1 (en) * 1995-03-10 2014-05-07 Meso Scale Technologies, LLC. Multi-array, multi-specific electrochemiluminescence testing
US6632436B2 (en) 1996-01-25 2003-10-14 Genitrix Llc Vaccine compositions and method of modulating immune responses
US6165739A (en) 1996-03-13 2000-12-26 Compucyte Corporation Multiple simultaneous testing media
US6039897A (en) 1996-08-28 2000-03-21 University Of Washington Multiple patterned structures on a single substrate fabricated by elastomeric micro-molding techniques
US6924153B1 (en) 1997-03-06 2005-08-02 Quidel Corporation Quantitative lateral flow assays and devices
US5858801A (en) 1997-03-13 1999-01-12 The United States Of America As Represented By The Secretary Of The Navy Patterning antibodies on a surface
US6103489A (en) 1997-03-21 2000-08-15 University Of Hawaii Cell-free protein synthesis system with protein translocation and processing
US6613512B1 (en) 1997-06-09 2003-09-02 Caliper Technologies Corp. Apparatus and method for correcting for variable velocity in microfluidic systems
US20030096232A1 (en) 1997-12-19 2003-05-22 Kris Richard M. High throughput assay system
US6210910B1 (en) * 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
US6316193B1 (en) 1998-10-06 2001-11-13 Origene Technologies, Inc. Rapid-screen cDNA library panels
US6429027B1 (en) * 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
DE60045917D1 (de) * 1999-02-23 2011-06-16 Caliper Life Sciences Inc Sequenzierung durch inkorporation
US8008071B2 (en) 1999-11-08 2011-08-30 University Of South Florida Compositions and methods for detecting intracellular glucose and analogs thereof
US6875619B2 (en) 1999-11-12 2005-04-05 Motorola, Inc. Microfluidic devices comprising biochannels
US20020090649A1 (en) 1999-12-15 2002-07-11 Tony Chan High density column and row addressable electrode arrays
US6699665B1 (en) * 2000-11-08 2004-03-02 Surface Logix, Inc. Multiple array system for integrating bioarrays
US7351575B2 (en) * 2000-11-08 2008-04-01 Surface Logix, Inc. Methods for processing biological materials using peelable and resealable devices
WO2002044695A1 (en) 2000-11-16 2002-06-06 Burstein Technologies, Inc. Methods and apparatus for detecting and quantifying lymphocytes with optical biodiscs
US20020100714A1 (en) 2001-01-31 2002-08-01 Sau Lan Tang Staats Microfluidic devices
US7968305B2 (en) 2001-03-24 2011-06-28 Aviva Biosciences Corporation Biochips including ion transport detecting structures and methods of use
US20020146745A1 (en) 2001-04-03 2002-10-10 Surromed, Inc. Methods and reagents for multiplexed analyte capture, surface array self-assembly, and analysis of complex biological samples
DE10127221A1 (de) 2001-05-23 2002-11-28 Lifebits Ag Träger für chemische, biochemische und biologische Substanzen
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
US7023007B2 (en) 2001-07-17 2006-04-04 Caliper Life Sciences, Inc. Methods and systems for alignment of detection optics
US10539561B1 (en) 2001-08-30 2020-01-21 Customarray, Inc. Enzyme-amplified redox microarray detection process
US7381375B2 (en) * 2001-10-26 2008-06-03 Millipore Corporation Assay systems with adjustable fluid communication
US20050032144A1 (en) 2001-11-16 2005-02-10 Lombardi Vincent C Method for selective conjugation of analytes to enzymes without unwanted enzyme-enzyme cross-linking
US7258837B2 (en) 2001-12-05 2007-08-21 University Of Washington Microfluidic device and surface decoration process for solid phase affinity binding assays
US20030190689A1 (en) 2002-04-05 2003-10-09 Cell Signaling Technology,Inc. Molecular profiling of disease and therapeutic response using phospho-specific antibodies
AU2002952384A0 (en) 2002-10-31 2002-11-14 Swinburne University Of Technology Structures
CN2577283Y (zh) * 2002-11-08 2003-10-01 穆海东 一种蛋白质芯片
DE10305050A1 (de) 2003-02-07 2004-08-19 Roche Diagnostics Gmbh Analytisches Testelement und Verfahren für Blutuntersuchungen
US7312197B2 (en) 2003-02-24 2007-12-25 University Of Maryland, Baltimore Method of modifying glucose activity using polypeptides selectively expressed in fat tissue
US7452678B2 (en) 2003-06-24 2008-11-18 Bristol-Myers Squibb Company Identification of biomarkers for liver toxicity
DE602004024539D1 (de) 2003-07-03 2010-01-21 Gentron Llc Verfahren und systeme zur diagnose von nicht das zentrale nervensystem (zns) betreffenden krankheiten in zns-proben
US20050226779A1 (en) 2003-09-19 2005-10-13 Oldham Mark F Vacuum assist for a microplate
US7981362B2 (en) 2003-11-04 2011-07-19 Meso Scale Technologies, Llc Modular assay plates, reader systems and methods for test measurements
JP4814103B2 (ja) 2003-11-12 2011-11-16 バイオ−ラッド・ハイファ・リミテッド アレイフォーマットにおいて複数の結合反応を実行するためのシステムおよび方法
JP4880484B2 (ja) 2004-02-20 2012-02-22 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 唾液ｍＲＮＡプロファイリング、バイオマーカーならびに関連した方法およびキット
JP4850061B2 (ja) 2004-03-18 2012-01-11 日水製薬株式会社 抗原の分析装置の製造方法及び分析装置
GB0409771D0 (en) * 2004-04-30 2004-06-09 Mabtech Ab Assay
DE102004056735A1 (de) * 2004-11-09 2006-07-20 Clondiag Chip Technologies Gmbh Vorrichtung für die Durchführung und Analyse von Mikroarray-Experimenten
CN1296492C (zh) 2004-11-18 2007-01-24 博奥生物有限公司 一种基于生物芯片检测能结合特异序列的核酸结合蛋白的方法
EP1838880A2 (en) 2005-01-21 2007-10-03 Third Wave Technologies, Inc. Methods and compositions for increased dynamic range detection of nucleic acid molecules
GB0508983D0 (en) 2005-05-03 2005-06-08 Oxford Gene Tech Ip Ltd Cell analyser
CA2607579A1 (en) 2005-05-06 2006-11-23 The Regents Of The University Of California Microfluidic system for identifying or sizing individual particles passing through a channel
US20060263818A1 (en) 2005-05-23 2006-11-23 Axel Scherer High throughput multi-antigen microfluidic fluorescence immunoassays
US20080220982A1 (en) 2005-07-26 2008-09-11 Vu Tania Q Nanoparticle Probes for Capture, Sorting and Placement of Targets
US20100297145A1 (en) 2005-08-04 2010-11-25 Kazutake Tsujikawa Apoptosis promoter, cell proliferation inhibitor, prophylactic/therapeutic agent for cancer, screening method for the promoter, inhibitor or agent
WO2007033385A2 (en) 2005-09-13 2007-03-22 Fluidigm Corporation Microfluidic assay devices and methods
WO2007035633A2 (en) * 2005-09-16 2007-03-29 President & Fellows Of Harvard College Screening assays and methods
US20070122819A1 (en) 2005-11-25 2007-05-31 Industrial Technology Research Institute Analyte assay structure in microfluidic chip for quantitative analysis and method for using the same
WO2007076023A2 (en) * 2005-12-21 2007-07-05 Meso Scale Technologies, Llc Assay modules having assay reagents and methods of making and using same
US20100009335A1 (en) 2006-02-07 2010-01-14 Victor Joseph Temperature-regulated culture plates
US8492098B2 (en) 2006-02-21 2013-07-23 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of reaction components that affect a reaction
AU2007281479A1 (en) 2006-08-02 2008-02-07 California Institute Of Technology Methods and systems for detecting and/or sorting targets
US8394590B2 (en) 2006-08-02 2013-03-12 California Institute Of Technology Capture agents and related methods and systems for detecting and/or sorting targets
WO2008101196A1 (en) 2007-02-15 2008-08-21 Osmetech Molecular Diagnostics Fluidics devices
JP2009002806A (ja) 2007-06-21 2009-01-08 Hitachi Ltd 化学発光計測装置
WO2009012340A2 (en) 2007-07-16 2009-01-22 California Institute Of Technology Microfluidic devices, methods and systems for detecting target molecules
WO2009105583A1 (en) 2008-02-19 2009-08-27 Anstron Technologies Company Fluorescence resonance energy transfer (fret) binding assays that are surface-based
US8871527B2 (en) 2008-04-29 2014-10-28 Becton, Dickinson And Company Method and system for measuring a sample to determine the presence of and optionally treat a pathologic condition
JP5777045B2 (ja) 2008-09-02 2015-09-09 国立研究開発法人産業技術総合研究所 細胞検出方法及び該方法に用いるマイクロアレイチップ
JP2010066146A (ja) 2008-09-11 2010-03-25 Panasonic Corp マイクロアレイ測定方法
KR101335725B1 (ko) * 2008-10-02 2013-12-04 삼성전자주식회사 다중분석용 미세유동 구조물 및 이를 포함하는 미세유동 장치
EP2370814B1 (en) * 2008-12-04 2015-04-29 Massachusetts Institute of Technology Method for diagnosing allergic reactions
US9244071B2 (en) * 2009-01-21 2016-01-26 Massachusetts Institute Of Technology Compositions and methods for assessing cytotoxicity of single cells
US8317737B2 (en) 2009-02-25 2012-11-27 The Invention Science Fund I, Llc Device for actively removing a target component from blood or lymph of a vertebrate subject
EP2473857B1 (en) 2009-09-01 2021-09-29 Corsolutions, LLC Microfluidic interface
US9500645B2 (en) 2009-11-23 2016-11-22 Cyvek, Inc. Micro-tube particles for microfluidic assays and methods of manufacture
WO2012022482A1 (en) * 2010-08-20 2012-02-23 Eth Zurich Microwell arrays for direct quantification of analytes on a flat sample
CN103917870B (zh) * 2011-11-16 2016-04-13 贝克顿·迪金森公司 用于检测样品中的分析物的方法和系统
CA2856868C (en) 2011-12-13 2020-07-07 Single Cell Technology, Inc. Method of screening a plurality of single secreting cells for functional activity
IN2014DN07531A (https=) 2012-03-30 2015-04-24 Clarient Diagnostic Services Inc
CN102690786B (zh) 2012-06-05 2014-02-12 武汉格蓝丽富科技有限公司 一种细胞富集、分离、提取的方法和仪器及单细胞分析方法
CN108267581A (zh) 2012-08-24 2018-07-10 耶鲁大学 用于高通量多重检测的系统、装置和方法
CA3088591C (en) 2012-09-28 2023-01-03 Vibrant Holdings, Llc Inverted pillar plates for assaying microarrays and methods of using same.
US20140307931A1 (en) 2013-04-15 2014-10-16 Massachusetts Institute Of Technology Fully automated system and method for image segmentation and quality control of protein microarrays
US20160122753A1 (en) 2013-06-12 2016-05-05 Tarjei Mikkelsen High-throughput rna-seq
EP3010550B1 (en) 2013-06-20 2021-10-27 California Institute of Technology Cyclic peptides as protein targeting agents
US10112195B2 (en) 2013-07-31 2018-10-30 Hitachi High-Technologies Corporation Flow cell for nucleic acid analysis and nucleic acid analyzer
US9498778B2 (en) 2014-11-11 2016-11-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
ES2895750T3 (es) 2014-09-15 2022-02-22 Abvitro Llc Secuenciación de alto rendimiento de colecciones de nucleótidos
US20160108458A1 (en) 2014-10-06 2016-04-21 The Board Of Trustees Of The Leland Stanford Junior University Multiplexed detection and quantification of nucleic acids in single-cells
KR20170068504A (ko) 2014-10-08 2017-06-19 노파르티스 아게 키메라 항원 수용체 요법에 대한 치료 반응성을 예측하는 바이오마커 및 그의 용도
CN112782140A (zh) 2014-12-03 2021-05-11 伊索普莱西斯公司 细胞分泌特征的分析和筛选
AU2015357526B2 (en) 2014-12-05 2022-03-17 Eureka Therapeutics, Inc. Chimeric antigen receptors targeting B-cell maturation antigen and uses thereof
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
CN108449933A (zh) 2015-11-18 2018-08-24 宝生物工程（美国)有限公司 用于汇集来自多孔装置的样品的系统和方法
CN110100010B (zh) 2016-07-18 2024-03-08 耶鲁大学 单细胞中细胞内或表面分子靶标的多重检测
EP3510398B1 (en) 2016-09-12 2026-02-25 Isoplexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
EP4036578A1 (en) 2016-11-11 2022-08-03 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
CN110226084A (zh) 2016-11-22 2019-09-10 伊索普莱克西斯公司 用于细胞捕获的系统、装置和方法，以及其制造方法
EP3580354A4 (en) 2017-02-13 2021-03-31 Yale University HIGH THROUGHPUT SINGLE CELL POLYOMICS
CA3056414A1 (en) 2017-03-16 2018-09-20 Case Western Reserve University Biochip having microchannel provided with capturing agent for performing cytological analysis

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