JP6088573B2 - クロマトグラフィー媒体用容器 - Google Patents
クロマトグラフィー媒体用容器 Download PDFInfo
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- JP6088573B2 JP6088573B2 JP2015074771A JP2015074771A JP6088573B2 JP 6088573 B2 JP6088573 B2 JP 6088573B2 JP 2015074771 A JP2015074771 A JP 2015074771A JP 2015074771 A JP2015074771 A JP 2015074771A JP 6088573 B2 JP6088573 B2 JP 6088573B2
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- 239000000539 dimer Substances 0.000 description 39
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 24
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- 238000011084 recovery Methods 0.000 description 15
- 239000011780 sodium chloride Substances 0.000 description 12
- 229910019142 PO4 Inorganic materials 0.000 description 11
- 239000002245 particle Substances 0.000 description 11
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 11
- 239000010452 phosphate Substances 0.000 description 11
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 10
- 238000007596 consolidation process Methods 0.000 description 10
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- 239000007858 starting material Substances 0.000 description 9
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- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
- 239000004471 Glycine Substances 0.000 description 5
- 239000012149 elution buffer Substances 0.000 description 5
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- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
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- 150000003839 salts Chemical class 0.000 description 3
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- DFCAFRGABIXSDS-UHFFFAOYSA-N Cycloate Chemical compound CCSC(=O)N(CC)C1CCCCC1 DFCAFRGABIXSDS-UHFFFAOYSA-N 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000012505 Superdex™ Substances 0.000 description 2
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- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 2
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- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
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- 239000006167 equilibration buffer Substances 0.000 description 1
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
- B01D15/206—Packing or coating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
- G01N30/56—Packing methods or coating methods
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6004—Construction of the column end pieces
- G01N30/6017—Fluid distributors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
- G01N30/6069—Construction of the column body with compartments or bed substructure
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
- G01N30/56—Packing methods or coating methods
- G01N2030/562—Packing methods or coating methods packing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
- G01N30/6082—Construction of the column body transparent to radiation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
- G01N30/6086—Construction of the column body form designed to optimise dispersion
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Peptides Or Proteins (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Description
a.上述のバッグをクロマトグラフィーカラムのチャンバー内に挿入する段階と、
b.カラムハウジングを閉鎖する段階と、
c.クロマトグラフィー媒体の充填ベッド又は圧密化ベッド又は流動ベッドを形成又は圧縮する段階と
を含む。
以下に詳述する実験は、2010年8月5日に出願された「Stabilization and Storage Media and Method」と題する本出願人による同時係属中の米国特許出願第61/370878号に十分に記載されている。その開示内容はその全体が、国内法が許容する場合、援用によって、具体的に記載されているのと同様に本明細書の内容の一部をなす。
最初に、ヒトポリクローナルIgGの単量体画分(1%二量体)を、VIVASPIN(商標)6限外濾過ユニットを用いて121.5AU(A280nm)に濃縮し、0.2μmシリンジフィルターで濾過した。各種の実験用捕獲(capture)保存ヒドロゲル媒体、及び対照(非捕獲SEC)媒体(上記材料参照)を96ウェルフィルタープレート(MULTITRAP(商標)プレート)に分配した。ヒトポリクローナルIgGの濃縮された単量体画分を抗体試料として用いてMULTITRAP(商標)プロトコルに従った。並行して、抗体試料を、その後の−20℃の溶液中の保存のためのいろいろな「ゲル媒体」として用いた各種の緩衝液と混合した。6.7μlの抗体溶液をそれぞれ13.3μlの各緩衝液と混合した。
・「pH5 IEX」=20mMの酢酸Na、0.02%(w/v)のNa−アジド、pH5.0
・「pH9 IEX」=20mMのNa−グリシン、0.02%(w/v)のNa−アジド、pH9.0
・「PBS」=10mMのリン酸Na、2.7mMのKCl、0.14MのNaCl、0.02%(w/v)のNa−アジド、pH7.4
・「pH5 HIC」=25mMの酢酸Na、0.5mMのEDTA、0.75M(NH4)2SO4、pH5.0。
・溶出緩衝液:「3.3×PBS」=30mMのリン酸Na、8.1mMのKCl、0.42MのNaCl、pH7.4(試料及びゲル媒体の平衡化/洗浄に使用した緩衝液が「pH5 IEX」及び「pH9 IEX」であった試料)、
・溶出緩衝液:0.1MのNa−グリシン pH2.9(試料及びゲル媒体の平衡化/洗浄に使用した緩衝液が「PBS」であった試料)、
・溶出及びストリップ緩衝液:20mMのTris−HCl pH7.5(試料及びゲル媒体の平衡化/洗浄に使用した緩衝液が「pH5 HIC」であった試料)、
・ストリップ緩衝液:10mMのNaOH、1MのNaCl(試料及びゲル媒体の平衡化/洗浄に使用した緩衝液が「pH5 IEX」及び「pH5 HIC」であった試料)、
・ストリップ緩衝液:0.5Mの酢酸(試料及びゲル媒体の平衡化/洗浄に使用した緩衝液が「PBS」及び「pH9 IEX」であった試料)。
ポリクローナルヒトIgG(GAMMANORM(登録商標)165mg/ml)を次の平衡化/洗浄緩衝液で30mg/mlに希釈した。
・20mM酢酸Na、0.02%(w/v)Na−アジド、pH5.0
・20mM Na−グリシン、0.02%(w/v)Na−アジド、pH9.0
・10mMリン酸Na、2.7mM KCl、0.14M NaCl、0.02%(w/v)Na−アジド、pH7.4
・50mM酢酸Na、1mM EDTA、1.5M (NH4)2SO4、pH5.0。
・CAPTO(商標)MMC混合モード媒体、20mMの酢酸Na、0.02%(w/v)のNa−アジド、pH5.0
・CAPTO(商標)Sカチオン交換媒体、20mMの酢酸Na、0.02%(w/v)のNa−アジド、pH5.0
・CAPTO(商標)Adhere混合モード媒体、20mMのNa−グリシン、0.02%(w/v)のNa−アジド、pH9.0
・CAPTO(商標)Qアニオン交換媒体、20mMのNa−グリシン、0.02%(w/v)のNa−アジド、pH9.0
・MABSELECT(商標)、親和性媒体、10mMのリン酸Na、2.7mMのKCl、0.14MのNaCl、0.02%(w/v)のNa−アジド、pH7.4
・SEPHADEX(商標)G−50、対照SEC媒体、10mMのリン酸Na、2.7mMのKCl、0.14MのNaCl、0.02%(w/v)のNa−アジド、pH7.4
・CAPTO(商標)Phe hs、フェニルリガンドを含有するHIC媒体、50mMの酢酸Na、1mMのEDTA、1.5Mの(NH4)2SO4、pH5.0
・pH HIC 6%、pH応答性ポリマー系のHIC媒体、50mMの酢酸Na、1mMのEDTA、1.5Mの(NH4)2SO4、pH5.0
・pH HIC 16%、pH応答性ポリマー系のHIC媒体、50mMの酢酸Na、1mMのEDTA、1.5Mの(NH4)2SO4、pH5.0。
・CAPTO(商標)MMC、20mMのリン酸Na、1MのNaCl、pH7.0
・CAPTO(商標)S、20mMのリン酸Na、1MのNaCl、pH7.0
・CAPTO(商標)Adhere、0.5Mの酢酸
・CAPTO(商標)Q、20mMのリン酸Na、1MのNaCl、pH7.0
・MABSELECT(商標)、0.5Mの酢酸
・SEPHADEX(商標)G−50、溶出しない、フロースルー画分を収集した
・CAPTO(商標)Phe hs、20mMのリン酸Na、1MのNaCl、pH7.0
・pH HIC 6%、20mMのリン酸Na、1MのNaCl、pH7.0
・pH HIC 16%、20mMのリン酸Na、1MのNaCl、pH7.0。
1.凝集に対するIgGの安定化(減速動力学)
2.IgG二量体の除去(洗練)
3.単量体形成の促進(単量体/二量体平衡の逆転)。
Claims (20)
- クロマトグラフィー媒体を収容する可撓性バッグであって、当該バッグが、
非多孔質材料の外壁であって、第1の液体分配要素及び第1の液体分配要素と対向する第2の液体分配要素が取り付けられて内部にクロマトグラフィー媒体用の区画を画成する非多孔質材料の外壁と、
媒体充填口と
を含んでおり、第1の液体分配要素及び/又は第2の液体分配要素が外壁に溶着又は成型されており、第1の液体分配要素及び第2の液体分配要素が、クロマトグラフィー媒体は通り抜けることができないほど小さいが液体は透過できる細孔を有するフィルター、メッシュ、ネット又は焼結材料から形成されており、前記バッグが円筒状構造を有していて、それぞれ第1及び第2の分配要素に隣接する第1の及び第2のエンドピースが外壁に取り付けられており、第1及び第2のエンドピースが共に担体液体用の入口又は出口を受容するための開口を含んでいて、第1及び第2のエンドピースが剛性又は半剛性材料製であって、外壁に溶着又は成型されている、バッグ。 - 第1の液体分配要素及び第1のエンドピースが一体化ユニットであり、第2の液体分配要素及び第2のエンドピースが一体化ユニットである、請求項1記載のバッグ。
- 軸方向クロマトグラフィー用の請求項1又は請求項2記載のバッグ。
- 前記バッグがさらに、液圧流体用の入口を有する第2の区画を含んでいる、請求項1及び請求項3のいずれか1項記載のバッグ。
- 前記壁がプラスチックポリマー材料からなる、請求項1乃至請求項4のいずれか1項記載のバッグ。
- さらに、壁の周りに半剛性ハウジングを含み、軸方向及び/又は半径方向の剛性を与える、請求項4乃至請求項5のいずれか1項記載のバッグ。
- 前記クロマトグラフィー媒体が湿潤又は乾燥状態である、請求項1乃至請求項6のいずれか1項記載のバッグ。
- 湿潤又は乾燥クロマトグラフィー媒体が無菌である、請求項7記載のバッグ。
- 前記バッグが放射線及び/又はオートクレーブ処理によって殺菌されている、請求項1乃至請求項8のいずれか1項記載のバッグ。
- クロマトグラフィーカラムを充填する方法であって、当該方法が、
a.請求項1乃至請求項9のいずれか1項記載のバッグをクロマトグラフィーカラムのチャンバー内に挿入する段階と、
b.カラムハウジングを閉鎖する段階と、
c.クロマトグラフィー媒体の充填ベッドを形成又は圧縮する段階と
を含んでおり、前記バッグが湿潤媒体を含有していて、カラムハウジングが剛性であり、バッグの軸方向の圧縮によって充填ベッドを形成又は圧縮する、方法。 - 前記バッグが乾燥クロマトグラフィー媒体を含有していて、液体を加えて媒体を膨潤させてカラムチャンバーの105〜120%の液体中膨潤体積Vsを与えることによって充填ベッドを形成する、請求項10記載の方法。
- 前記カラムが液圧チャンバーを含んでおり、チャンバーに液圧流体を満たすことによって充填ベッドを形成又は圧縮する、請求項10記載の方法。
- 前記液圧チャンバーが可撓性バッグである、請求項12記載の方法。
- 前記バッグが第2の区画を含んでおり、第2の区画に液圧流体を満たすことによって充填ベッドを形成又は圧縮する、請求項10記載の方法。
- 前記カラムが可動性ピストン又はアダプターを含んでおり、ピストン又はアダプターの軸方向の移動によって充填ベッドを形成又は圧縮する、請求項10記載の方法。
- 湿潤媒体の体積圧縮が5〜20%である、請求項10及び請求項15のいずれか1項記載の方法。
- さらに、カラムを放射線で殺菌することを含む、請求項10乃至請求項16のいずれか1項記載の方法。
- 請求項1乃至請求項9のいずれか1項記載のバッグを備えるクロマトグラフィーカラム。
- 化学物質の分離又は精製に使用するための請求項18記載のクロマトグラフィーカラム。
- 前記化学物質がタンパク質である、請求項19記載のクロマトグラフィーカラム。
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Free format text: JAPANESE INTERMEDIATE CODE: R250 |
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Free format text: JAPANESE INTERMEDIATE CODE: R250 |
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Free format text: JAPANESE INTERMEDIATE CODE: R250 |