JP6002763B2 - グラム陰性細菌の外膜小胞を、洗浄剤を含まずに生産するためのプロセス - Google Patents
グラム陰性細菌の外膜小胞を、洗浄剤を含まずに生産するためのプロセス Download PDFInfo
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Description
a)グラム陰性細菌の集団を、定常増殖期まで培養するステップと、
b)定常増殖期の開始後少なくとも約1時間の時点で、a)において得られた細菌を、pHが約pH7.5を超えるか若しくはpH8.0を超えるように調整されるか、又は約pH7.5を超えるか若しくはpH8.0を超え、金属キレート化剤、好ましくはEDTAの濃度が約1から100mMの間となるように調整されるか又は約1から100mMの間である培地中でインキュベートして、OMVを抽出するステップと、
c)b)において抽出されたOMVを回収するステップであって、回収が、OMVからの細菌の除去を少なくとも含むステップと
を含むプロセスを提供する。
を有することが好ましい。
収率及び純度が改善された、髄膜炎菌血清型Bに対する九価のOMVワクチンの規模拡大可能な製造
以下の例は、40Lの産生用培養物により実施したが、少なくとも800Lまでは完全に規模拡大可能である。
株RL16.2.15が、P1.7,16/P1.5−1,2−2/P1.19,15−1を発現し、
株RL10.12.4が、P1.5−2,10/P12−1,13/P1.7−2,4を発現し、
株RL14.1.6が、P1.22,14/P1.7−1,1/P1.18−1,3,6を発現する髄膜炎菌血清群B株
を、−135℃で、マスターの種地として保管した。マスターの種地を融解させ、150mLの既知組成培地[Baartら、2007]を伴う振とうフラスコ内で増殖させ、指数関数的増殖の間にアリコートに分割し、グリセロールを添加した後−135℃で保管し、作業用種地を得た。
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Claims (24)
- ワクチンにおいて用いられる細菌の外膜小胞(OMV)を、洗浄剤を含まずに調製するためのプロセスであって、
a)グラム陰性細菌の集団を、定常増殖期まで培養するステップと、
b)定常増殖期の開始後少なくとも1時間の時点で、a)において得られた細菌を、pHがpH8.0を超えるように調整されるか又はpH8.0を超え、かつ金属キレート化剤の濃度が1から100mMの間となるように調整されるか又は1から100mMの間である培地中でインキュベートして、OMVを抽出するステップと、
c)b)において抽出されたOMVを回収するステップであって、回収が、OMVからの細菌の除去を少なくとも含むステップと
を含む上記プロセス。 - 前記金属キレート化剤がEDTAである、請求項1に記載のプロセス。
- グラム陰性細菌が、細菌に毒性が低減されたLPSを産生させるが、LPSがそのアジュバント活性の少なくとも一部を保持する遺伝子改変を有する、請求項1又は2に記載のプロセス。
- 前記遺伝子改変が、lpxL1遺伝子及びlpxL2遺伝子若しくはこれらの相同体、並びにlpxK遺伝子若しくはその相同体から選択される1又は複数の遺伝子の発現を減少させるか、若しくはこれをノックアウトする改変であり、並びに/又は1又は複数のlpxE遺伝子及び/若しくはpagL遺伝子の発現をもたらす改変である、請求項3に記載のプロセス。
- b)において、細菌を培地中でインキュベートする定常増殖期の開始後の時点が、1から9時間の間である、請求項1から4までのいずれか一項に記載のプロセス。
- 前記時点が2から5時間の間である、請求項5に記載のプロセス。
- OMVを滅菌する、請求項1から6までのいずれか一項に記載のプロセス。
- OMVを濾過滅菌により滅菌する、請求項7に記載のプロセス。
- OMVを小孔が0.3マイクロメートル未満のフィルターを用いて濾過滅菌により滅菌する、請求項8に記載のプロセス。
- ステップb)における金属キレート化剤の濃度が、5から15mMの間であり、及び/又は、pHが、pH8.0からpH9.5の間である、請求項1から9までのいずれか一項に記載のプロセス。
- 前記pHが、pH8.0からpH9.0の間である、請求項10に記載のプロセス。
- 前記pHが、pH8.2からpH8.8の間である、請求項11に記載のプロセス。
- 前記pHが、pH8.4からpH8.7の間である、請求項12に記載のプロセス。
- 前記金属キレート化剤がEDTAである、請求項10から13までのいずれか一項に記載のプロセス。
- a)における培養物の容量、及び/又はb)における培地の容量が、少なくとも10Lである、請求項1から14までのいずれか一項に記載のプロセス。
- 前記a)における培養物の容量、及び/又はb)における培地の容量が、少なくとも20L、40L、60L、80L、100L、200L、300L、400L、500L、800L、1500L、5000L、10,000L、20,000L、又は40,000Lである、請求項15に記載のプロセス。
- グラム陰性細菌が、ナイセリア(Neisseria)属又はボルデテラ(Bordetella)属の種である、請求項1から16までのいずれか一項に記載のプロセス。
- 前記グラム陰性細菌が、髄膜炎菌(Neisseria meningitidis)又は百日咳菌(Bordetella pertussis)である、請求項17に記載のプロセス。
- グラム陰性細菌が、遺伝子産物の発現を減少させるか、又はノックアウトする1又は複数の突然変異を有する、請求項1から18までのいずれか一項に記載のプロセス。
- 前記遺伝子産物が、cpsと、lpxL1、rmpM、porA、porB、及びopAを含むリピドA生合成遺伝子産物からなる群から選択される、請求項19に記載のプロセス。
- グラム陰性細菌が、複数のporA亜型を発現する、請求項1から20までのいずれか一項に記載のプロセス。
- 集団が、グラム陰性細菌の複数の株を含み、各株が、異なるporA亜型を発現する、請求項1から21までのいずれか一項に記載のプロセス。
- グラム陰性細菌が、前記グラム陰性細菌に対する外来の抗原を発現する、請求項1から22までのいずれか一項に記載のプロセス。
- OMVを、医薬として許容される賦形剤と組み合わせるステップをさらに含む、請求項1から23までのいずれか一項に記載のプロセス。
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PCT/NL2012/050478 WO2013006055A1 (en) | 2011-07-07 | 2012-07-05 | A process for detergent-free production of outer membrane vesicles |
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EP3327028B1 (en) | 2010-03-30 | 2022-03-16 | Children's Hospital & Research Center at Oakland | Factor h binding proteins (fhbp) with altered properties and methods of use thereof |
RS57267B1 (sr) * | 2011-07-07 | 2018-08-31 | De Staat Der Nederlanden Vert Door De Minister Van Vws | Postupak za proizvodnju vezikula spoljašnje membrane gram-negativne bakterije bez upotrebe deterdženta |
US10279026B2 (en) * | 2012-04-26 | 2019-05-07 | Glaxosmithkline Biologicals Sa | Antigens and antigen combinations |
EA201690056A8 (ru) * | 2013-08-02 | 2017-07-31 | Чилдренс Хоспитл Энд Рисерч Сентер Эт Окленд | ИСКУССТВЕННЫЕ БЕЛКИ, СВЯЗЫВАЮЩИЕ ФАКТОР Н (fHbp), И СПОСОБЫ ИХ ПРИМЕНЕНИЯ |
WO2016014719A1 (en) | 2014-07-23 | 2016-01-28 | Children's Hospital & Research Center Oakland | Factor h binding protein variants and methods of use thereof |
KR101715366B1 (ko) * | 2014-09-04 | 2017-03-14 | 한국과학기술연구원 | 지질의 탈인산화용 조성물, 키트, 및 이를 이용한 탈인산화된 지질을 생산하는 방법 |
EP3223847A1 (en) * | 2014-11-28 | 2017-10-04 | Janssen Vaccines & Prevention B.V. | Meningitis b vaccine |
CN117247460A (zh) * | 2015-06-02 | 2023-12-19 | Intravacc有限责任公司 | 革兰氏阴性菌外膜囊泡抗原表面展示 |
US11292808B2 (en) | 2016-01-28 | 2022-04-05 | Intravacc B.V. | Modified hexa-acylated neisserial LPS |
JP7031933B2 (ja) * | 2016-01-28 | 2022-03-08 | イントラヴァック ビー.ブイ. | 改変されたテトラアシル化ナイセリアlps |
BE1025210B1 (fr) * | 2016-11-25 | 2018-12-12 | Glaxosmithkline Biologicals Sa | Conjugues immunogenes et leur utilisation |
CN110461865A (zh) | 2017-03-13 | 2019-11-15 | 由卫生福利和体育大臣代表的荷兰王国 | 包含具有降低的反应原性的lps的博德特氏菌疫苗 |
BR112020011019A2 (pt) * | 2017-12-04 | 2020-11-17 | De Staat Der Nederlanden, Vert. Door De Minister Van Vws, Ministerie Van Volksgezondheid, Welzijn En Sport | processo aprimorado para produzir vesículas de membrana externa |
JP2022520999A (ja) * | 2019-02-22 | 2022-04-04 | エヴェロ バイオサイエンシズ,インコーポレーテッド | 細菌の膜調製物 |
US20230270849A1 (en) | 2020-05-08 | 2023-08-31 | Intravacc B.V. | click OMVs |
EP4208472A1 (en) | 2020-09-04 | 2023-07-12 | Intravacc B.V. | Ompa mutations enhance omv production in bordetella pertussis |
CN115216478B (zh) * | 2022-06-01 | 2024-03-26 | 华南农业大学 | 一种禽多杀性巴氏杆菌内毒素减毒灭活疫苗菌株的构建方法及应用 |
CN115976088B (zh) * | 2022-07-21 | 2023-09-19 | 深圳蓝晶生物科技有限公司 | 低内毒素含量的罗氏真养菌及其应用 |
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US5997881A (en) | 1995-11-22 | 1999-12-07 | University Of Maryland, Baltimore | Method of making non-pyrogenic lipopolysaccharide or A |
US6558677B2 (en) * | 1996-10-15 | 2003-05-06 | Wendell D. Zollinger | Vaccine against gram negative bacteria |
AU762369B2 (en) | 1998-11-03 | 2003-06-26 | De Staat Der Nederlanden Vertegenwoordigd Door De Minister Van Welzijn, Volksgezonheid En Cultuur | LPS with reduced toxicity from genetically modified gram negative bacteria |
GB0103170D0 (en) * | 2001-02-08 | 2001-03-28 | Smithkline Beecham Biolog | Vaccine composition |
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SI1748791T1 (sl) | 2004-05-11 | 2010-07-30 | Staat Der Nederlanden - Minister Van Vws | Neisseria meningitidis IgtB LOS kot adjuvans |
EP2682126B1 (en) * | 2005-01-27 | 2016-11-23 | Children's Hospital & Research Center at Oakland | GNA1870-based vesicle vaccines for broad spectrum protection against diseases caused by Neisseria meningitidis |
RS57267B1 (sr) * | 2011-07-07 | 2018-08-31 | De Staat Der Nederlanden Vert Door De Minister Van Vws | Postupak za proizvodnju vezikula spoljašnje membrane gram-negativne bakterije bez upotrebe deterdženta |
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AU2012278407B2 (en) | 2017-01-19 |
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CA2840096C (en) | 2021-07-06 |
DK2729167T3 (en) | 2018-04-30 |
PT2729167T (pt) | 2018-06-20 |
US9272006B2 (en) | 2016-03-01 |
US9707286B2 (en) | 2017-07-18 |
PL2729167T3 (pl) | 2018-08-31 |
WO2013006055A1 (en) | 2013-01-10 |
AU2012278407A1 (en) | 2014-01-16 |
LT2729167T (lt) | 2018-06-25 |
CN103687612A (zh) | 2014-03-26 |
RS57267B1 (sr) | 2018-08-31 |
WO2013006055A8 (en) | 2013-08-15 |
CA2840096A1 (en) | 2013-01-10 |
EP2729167A1 (en) | 2014-05-14 |
EP2729167B1 (en) | 2018-03-14 |
US20160303214A1 (en) | 2016-10-20 |
ES2670669T3 (es) | 2018-05-31 |
US20140147469A1 (en) | 2014-05-29 |
CN103687612B (zh) | 2017-12-15 |
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