JP5963683B2 - カプセル化された小滴に関するエマルジョンの化学およびアッセイ - Google Patents
カプセル化された小滴に関するエマルジョンの化学およびアッセイ Download PDFInfo
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- JP5963683B2 JP5963683B2 JP2012556218A JP2012556218A JP5963683B2 JP 5963683 B2 JP5963683 B2 JP 5963683B2 JP 2012556218 A JP2012556218 A JP 2012556218A JP 2012556218 A JP2012556218 A JP 2012556218A JP 5963683 B2 JP5963683 B2 JP 5963683B2
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- Prior art keywords
- emulsion
- droplets
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- capsule
- continuous phase
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Manufacturing Of Micro-Capsules (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (21)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US30984510P | 2010-03-02 | 2010-03-02 | |
| US61/309,845 | 2010-03-02 | ||
| US31763510P | 2010-03-25 | 2010-03-25 | |
| US34121810P | 2010-03-25 | 2010-03-25 | |
| US61/341,218 | 2010-03-25 | ||
| US61/317,635 | 2010-03-25 | ||
| US38098110P | 2010-09-08 | 2010-09-08 | |
| US61/380,981 | 2010-09-08 | ||
| US40910610P | 2010-11-01 | 2010-11-01 | |
| US61/409,106 | 2010-11-01 | ||
| US40947310P | 2010-11-02 | 2010-11-02 | |
| US61/409,473 | 2010-11-02 | ||
| US41076910P | 2010-11-05 | 2010-11-05 | |
| US61/410,769 | 2010-11-05 | ||
| US41724110P | 2010-11-25 | 2010-11-25 | |
| US61/417,241 | 2010-11-25 | ||
| US12/976,827 | 2010-12-22 | ||
| US12/976,827 US9598725B2 (en) | 2010-03-02 | 2010-12-22 | Emulsion chemistry for encapsulated droplets |
| US12/976,816 | 2010-12-22 | ||
| US12/976,816 US8399198B2 (en) | 2010-03-02 | 2010-12-22 | Assays with droplets transformed into capsules |
| PCT/US2011/026901 WO2011109546A1 (en) | 2010-03-02 | 2011-03-02 | Emulsion chemistry and assays for encapsulated droplets |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016022474A Division JP6188844B2 (ja) | 2010-03-02 | 2016-02-09 | カプセル化された小滴に関するエマルジョンの化学およびアッセイ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013520997A JP2013520997A (ja) | 2013-06-10 |
| JP2013520997A5 JP2013520997A5 (https=) | 2014-04-24 |
| JP5963683B2 true JP5963683B2 (ja) | 2016-08-03 |
Family
ID=44531671
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012556218A Active JP5963683B2 (ja) | 2010-03-02 | 2011-03-02 | カプセル化された小滴に関するエマルジョンの化学およびアッセイ |
| JP2016022474A Active JP6188844B2 (ja) | 2010-03-02 | 2016-02-09 | カプセル化された小滴に関するエマルジョンの化学およびアッセイ |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016022474A Active JP6188844B2 (ja) | 2010-03-02 | 2016-02-09 | カプセル化された小滴に関するエマルジョンの化学およびアッセイ |
Country Status (5)
| Country | Link |
|---|---|
| US (6) | US9598725B2 (https=) |
| EP (4) | EP2542660B1 (https=) |
| JP (2) | JP5963683B2 (https=) |
| CA (3) | CA2996219C (https=) |
| WO (1) | WO2011109546A1 (https=) |
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| US12090480B2 (en) | 2008-09-23 | 2024-09-17 | Bio-Rad Laboratories, Inc. | Partition-based method of analysis |
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| US12162008B2 (en) | 2008-09-23 | 2024-12-10 | Bio-Rad Laboratories, Inc. | Partition-based method of analysis |
| US9399215B2 (en) | 2012-04-13 | 2016-07-26 | Bio-Rad Laboratories, Inc. | Sample holder with a well having a wicking promoter |
| US11130128B2 (en) | 2008-09-23 | 2021-09-28 | Bio-Rad Laboratories, Inc. | Detection method for a target nucleic acid |
| US9132394B2 (en) | 2008-09-23 | 2015-09-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
| US20130084572A1 (en) * | 2011-09-30 | 2013-04-04 | Quantalife, Inc. | Calibrations and controls for droplet-based assays |
| CA2738578C (en) | 2008-09-23 | 2021-05-04 | Quantalife, Inc. | Droplet-based assay system |
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