JP5931738B2 - 新規のペプチジルアルファ−ヒドロキシグリシンアルファ−アミド化リアーゼ - Google Patents

新規のペプチジルアルファ−ヒドロキシグリシンアルファ−アミド化リアーゼ Download PDF

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Publication number
JP5931738B2
JP5931738B2 JP2012541487A JP2012541487A JP5931738B2 JP 5931738 B2 JP5931738 B2 JP 5931738B2 JP 2012541487 A JP2012541487 A JP 2012541487A JP 2012541487 A JP2012541487 A JP 2012541487A JP 5931738 B2 JP5931738 B2 JP 5931738B2
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enzyme
xaa
polypeptide
peptide
seq
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JP2012541487A
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Japanese (ja)
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JP2013511997A5 (enExample
JP2013511997A (ja
Inventor
アラン・クリスチャン・ショー
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Novo Nordisk AS
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Novo Nordisk AS
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00Drugs for disorders of the metabolism
    • A61P3/04Anorexiants; Antiobesity agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00Drugs for disorders of the metabolism
    • A61P3/08Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y403/00Carbon-nitrogen lyases (4.3)
    • C12Y403/02Amidine-lyases (4.3.2)
    • C12Y403/02005Peptidylamidoglycolate lyase (4.3.2.5)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Diabetes (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Public Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Emergency Medicine (AREA)
  • Child & Adolescent Psychology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2012541487A 2009-12-01 2010-12-01 新規のペプチジルアルファ−ヒドロキシグリシンアルファ−アミド化リアーゼ Expired - Fee Related JP5931738B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP09177593.2 2009-12-01
EP09177593 2009-12-01
US26671109P 2009-12-04 2009-12-04
US61/266,711 2009-12-04
PCT/EP2010/068630 WO2011067283A1 (en) 2009-12-01 2010-12-01 Novel peptidyl alpha-hydroxyglycine alpha-amidating lyases

Publications (3)

Publication Number Publication Date
JP2013511997A JP2013511997A (ja) 2013-04-11
JP2013511997A5 JP2013511997A5 (enExample) 2014-01-16
JP5931738B2 true JP5931738B2 (ja) 2016-06-08

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012541487A Expired - Fee Related JP5931738B2 (ja) 2009-12-01 2010-12-01 新規のペプチジルアルファ−ヒドロキシグリシンアルファ−アミド化リアーゼ

Country Status (6)

Country Link
US (2) US9096843B2 (enExample)
EP (1) EP2507258B1 (enExample)
JP (1) JP5931738B2 (enExample)
CN (1) CN102666570B (enExample)
ES (1) ES2583259T3 (enExample)
WO (1) WO2011067283A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105793278A (zh) * 2013-12-20 2016-07-20 豪夫迈·罗氏有限公司 肽基甘氨肽α酰胺化单氧加氧酶(PAM)用于C端酰胺化的用途
CN105985995A (zh) * 2015-01-29 2016-10-05 暨南大学 一种利用家蚕生物反应器生产普兰林肽(pa)的方法
CN113302310B (zh) * 2018-12-18 2024-05-03 帕西昂奥地利有限两合公司 单步生物催化酰胺化
CN110034855B (zh) * 2019-04-10 2021-12-14 国网辽宁省电力有限公司 一种信息传输校验方法及系统
JP2023515981A (ja) * 2020-02-26 2023-04-17 ペーアーエム セラノスティクス ゲゼルシャフト ミット ベシュレンクテル ハフツング ペプチジルグリシンαアミド化モノオキシゲナーゼ(PAM)を決定する方法および診断目的でのその使用
IL295924A (en) * 2020-02-26 2022-10-01 Pam Theragnostics Gmbh Use of peptidylglycine alpha-amidization monooxygenase (pam) for medicinal purposes
US20230167153A1 (en) 2020-05-01 2023-06-01 Kashiv Biosciences, Llc An improved process of purification of protein
EP4487124A1 (en) * 2022-03-23 2025-01-08 Kashiv Biosciences, LLC An improved process for the separation and analysis of pancreatic enzymes
WO2025133225A1 (en) * 2023-12-22 2025-06-26 Pam Theragnostics Gmbh Pharmaceutical combination of peptide-gly and peptidylglycine alpha-amidating monooxygenase (pam)

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4203570A (en) 1978-08-23 1980-05-20 The Western States Machine Company Power-operated loading gate for centrifugal machines incorporating an auxiliary drive device
US4546082A (en) 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
US4599311A (en) 1982-08-13 1986-07-08 Kawasaki Glenn H Glycolytic promotersfor regulated protein expression: protease inhibitor
WO1984004330A1 (en) 1983-04-22 1984-11-08 Amgen Secretion of exogenous polypeptides from yeast
NZ207926A (en) 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
US4870008A (en) 1983-08-12 1989-09-26 Chiron Corporation Secretory expression in eukaryotes
US4931373A (en) 1984-05-25 1990-06-05 Zymogenetics, Inc. Stable DNA constructs for expression of α-1 antitrypsin
DK58285D0 (da) 1984-05-30 1985-02-08 Novo Industri As Peptider samt fremstilling og anvendelse deraf
US4708934A (en) 1984-09-27 1987-11-24 Unigene Laboratories, Inc. α-amidation enzyme
US4766073A (en) 1985-02-25 1988-08-23 Zymogenetics Inc. Expression of biologically active PDGF analogs in eucaryotic cells
US4885249A (en) 1984-12-05 1989-12-05 Allelix, Inc. Aspergillus niger transformation system
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
AU607398B2 (en) 1985-08-29 1991-03-07 Genencor Inc. Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same
US4882279A (en) 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
HU206897B (en) 1985-10-25 1993-01-28 Zymogenetics Inc Process for utilizing bar-1 gen for selecting strange proteins
US4935349A (en) 1986-01-17 1990-06-19 Zymogenetics, Inc. Expression of higher eucaryotic genes in aspergillus
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
GB8720115D0 (en) * 1987-08-26 1987-09-30 Cooper G J S Treatment of diabetes mellitus
US5789234A (en) 1987-08-14 1998-08-04 Unigene Laboratories, Inc. Expression systems for amidating enzyme
JP2703598B2 (ja) 1987-09-04 1998-01-26 ノボ―ノルディスク アクティーゼルスカブ アスペルギルス菌中でのタンパク質生産物の生産方法およびアスペルギルス菌中で使用するためのプロモーター
DK463887D0 (da) 1987-09-07 1987-09-07 Novo Industri As Gaerleader
US6255067B1 (en) 1987-09-15 2001-07-03 The Johns Hopkins University cDNA encoding peptidyl-glycine alpha-amidating monooxygenase (PAM)
US5037743A (en) 1988-08-05 1991-08-06 Zymogenetics, Inc. BAR1 secretion signal
JP2845468B2 (ja) * 1989-01-17 1999-01-13 サントリー株式会社 ヒト甲状腺由来C―末端α―アミド化酵素
JP2535398B2 (ja) 1989-01-17 1996-09-18 サントリー株式会社 アミド化ペプチドの製造方法
EP0448513A3 (en) 1990-03-21 1991-12-27 Japat Ltd Process for production of peptidylglycine alpha-hydroxylating monooxygenase and use thereof
EP0465404B1 (en) * 1990-06-01 1995-11-15 Ciba-Geigy Ag Peptdidylhydroxylglycine N-C lyase and DNA coding therefor
DK300090D0 (da) 1990-12-19 1990-12-19 Novo Nordisk As Fremgangsmaade til fremstilling af leadersekvenser
US6495514B1 (en) 1998-01-21 2002-12-17 Mercer University Method for reducing inflammation and inducing an analgesic effect and compounds thereof
WO2001049856A2 (en) * 2000-01-06 2001-07-12 Genoptera, Llc Drosophila enzymes, encoding nucleic acids and methods of use
WO2002048192A2 (en) * 2000-12-13 2002-06-20 Eli Lilly And Company Amidated glucagon-like peptide-1
PL1651249T3 (pl) * 2003-07-23 2013-04-30 Novartis Ag Zastosowanie kalcytoniny w zapaleniu kości i stawów
EP1874798A1 (en) 2005-04-15 2008-01-09 Novo Nordisk A/S Basic protein purification tags from thermophilic bacteria
US8815583B2 (en) 2005-06-24 2014-08-26 Enteris Biopharma, Inc. Cell lines for expressing enzyme useful in the preparation of amidated products
CN101563358A (zh) 2006-10-13 2009-10-21 诺沃-诺迪斯克保健股份有限公司 融合于碱性蛋白标签的加工酶
WO2009005140A1 (ja) 2007-06-29 2009-01-08 Asubio Pharma Co., Ltd. 組換えC-末端α-アミド化酵素誘導体

Also Published As

Publication number Publication date
EP2507258B1 (en) 2016-04-20
WO2011067283A1 (en) 2011-06-09
CN102666570A (zh) 2012-09-12
ES2583259T3 (es) 2016-09-20
US20160083713A1 (en) 2016-03-24
JP2013511997A (ja) 2013-04-11
CN102666570B (zh) 2015-12-02
EP2507258A1 (en) 2012-10-10
US20120322733A1 (en) 2012-12-20
US9096843B2 (en) 2015-08-04

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