JP5907864B2 - ステム加速等温核酸増幅技術 - Google Patents

ステム加速等温核酸増幅技術 Download PDF

Info

Publication number
JP5907864B2
JP5907864B2 JP2012515553A JP2012515553A JP5907864B2 JP 5907864 B2 JP5907864 B2 JP 5907864B2 JP 2012515553 A JP2012515553 A JP 2012515553A JP 2012515553 A JP2012515553 A JP 2012515553A JP 5907864 B2 JP5907864 B2 JP 5907864B2
Authority
JP
Japan
Prior art keywords
primer
stem
primers
region
segment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2012515553A
Other languages
English (en)
Japanese (ja)
Other versions
JP2012529904A5 (cg-RX-API-DMAC7.html
JP2012529904A (ja
Inventor
ローレンス・カルロ・ティシ
オルガ・ガンデルマン
ガイ・キドル
キャサル・ジョゼフ・マクエルガン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lumora Ltd
Original Assignee
Lumora Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lumora Ltd filed Critical Lumora Ltd
Publication of JP2012529904A publication Critical patent/JP2012529904A/ja
Publication of JP2012529904A5 publication Critical patent/JP2012529904A5/ja
Application granted granted Critical
Publication of JP5907864B2 publication Critical patent/JP5907864B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2012515553A 2009-06-15 2010-06-15 ステム加速等温核酸増幅技術 Expired - Fee Related JP5907864B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0910302.9A GB0910302D0 (en) 2009-06-15 2009-06-15 Nucleic acid amplification
GB0910302.9 2009-06-15
PCT/GB2010/001169 WO2010146349A1 (en) 2009-06-15 2010-06-15 Stem accelerated isothermal nucleic acid amplification technology

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2015201347A Division JP6106732B2 (ja) 2009-06-15 2015-10-09 ステム加速等温核酸増幅技術

Publications (3)

Publication Number Publication Date
JP2012529904A JP2012529904A (ja) 2012-11-29
JP2012529904A5 JP2012529904A5 (cg-RX-API-DMAC7.html) 2013-08-01
JP5907864B2 true JP5907864B2 (ja) 2016-04-26

Family

ID=40940851

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2012515553A Expired - Fee Related JP5907864B2 (ja) 2009-06-15 2010-06-15 ステム加速等温核酸増幅技術
JP2015201347A Expired - Fee Related JP6106732B2 (ja) 2009-06-15 2015-10-09 ステム加速等温核酸増幅技術

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2015201347A Expired - Fee Related JP6106732B2 (ja) 2009-06-15 2015-10-09 ステム加速等温核酸増幅技術

Country Status (10)

Country Link
US (1) US9410190B2 (cg-RX-API-DMAC7.html)
EP (1) EP2443253B1 (cg-RX-API-DMAC7.html)
JP (2) JP5907864B2 (cg-RX-API-DMAC7.html)
CN (1) CN102652176B (cg-RX-API-DMAC7.html)
AU (1) AU2010261541B2 (cg-RX-API-DMAC7.html)
CA (1) CA2765406C (cg-RX-API-DMAC7.html)
ES (1) ES2589079T3 (cg-RX-API-DMAC7.html)
GB (1) GB0910302D0 (cg-RX-API-DMAC7.html)
WO (1) WO2010146349A1 (cg-RX-API-DMAC7.html)
ZA (1) ZA201109493B (cg-RX-API-DMAC7.html)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI1007214A8 (pt) 2009-01-30 2022-12-20 Oxford Nanopore Tech Ltd Adaptador para sequenciar ácidos nucleicos, par de adaptadores, kit, construto de ácido nucleico, e, métodos para preparar um adaptador, um construto de ácido nucleico, e um construto de dequência, e para sequenciar ácido nucleico de fita dupla.
EP3144396B1 (en) 2010-10-27 2020-01-01 President and Fellows of Harvard College Methods of use of toehold hairpin primer
WO2013048583A2 (en) * 2011-05-24 2013-04-04 Elitech Holding B.V. Detection of methicillin-resistant staphylococcus aureus
CA2843136C (en) 2011-07-25 2020-12-29 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
CN105209634B (zh) 2013-03-08 2020-05-12 牛津纳米孔技术公司 酶停滞方法
AU2014233150A1 (en) 2013-03-15 2015-09-17 Theranos Ip Company, Llc Nucleic acid amplification
CA2906824C (en) 2013-03-15 2023-10-03 Theranos, Inc. Nucleic acid amplification
WO2014151177A1 (en) 2013-03-15 2014-09-25 3M Innovative Properties Company Sample concentrator and method of use
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
CN105452480B (zh) * 2013-08-09 2020-01-31 基因生物系统股份有限公司 经由剪刀状结构的dna扩增(dasl)
SG11201601611WA (en) 2013-09-06 2016-04-28 Theranos Inc Systems and methods for detecting infectious diseases
CN103497947B (zh) * 2013-09-13 2015-08-12 浙江国际旅行卫生保健中心 一种体外新型快速环介导等温扩增方法、体外检测甲型h1n1流感病毒的试剂及方法
EP3071713B1 (en) * 2013-11-22 2021-05-12 Labrador Diagnostics LLC Nucleic acid amplification
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
EP3495506B1 (en) 2013-12-11 2023-07-12 AccuraGen Holdings Limited Methods for detecting rare sequence variants
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10716587B2 (en) 2014-06-13 2020-07-21 Surgis Medical Llc Surgical device with light
EP3169793B1 (en) * 2014-07-16 2022-07-06 Tangen Biosciences Inc. Isothermal methods for amplifying nucleic acid samples
WO2016073353A1 (en) 2014-11-03 2016-05-12 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
US11639522B2 (en) 2015-01-30 2023-05-02 President And Fellows Of Harvard College Microscope-free imaging
US10612073B2 (en) 2015-02-26 2020-04-07 Hitachi High-Technologies Corporation Method for constructing nucleic acid molecule
US10260090B2 (en) 2015-07-16 2019-04-16 Arizona Board Of Regents On Behalf Of Arizona State University Accelerated isothermal amplification of DNA
KR20180055905A (ko) * 2015-10-09 2018-05-25 아큐라젠 홀딩스 리미티드 증폭 산물의 농축을 위한 방법 및 조성물
WO2017096322A1 (en) 2015-12-03 2017-06-08 Accuragen Holdings Limited Methods and compositions for forming ligation products
US11286517B2 (en) 2016-02-17 2022-03-29 President And Fellows Of Harvard College Molecular programming tools
WO2017201102A1 (en) 2016-05-16 2017-11-23 Accuragen Holdings Limited Method of improved sequencing by strand identification
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN107488656B (zh) * 2016-06-13 2020-07-17 陆欣华 一种核酸等温自扩增方法
CN109844133A (zh) 2016-08-15 2019-06-04 安可济控股有限公司 检测罕见序列变体的组合物和方法
EP3568492B1 (en) * 2017-01-10 2025-01-01 President and Fellows of Harvard College Multiplexed signal amplification
EP3724322A1 (en) 2017-12-14 2020-10-21 Medicinal Genomics Corporation Methods and kits for classifying cannabinoid production in cannabis plants
CN111630179B (zh) 2018-01-26 2024-07-19 哈佛学院院长及董事 邻近检测方法和组合物
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
CN112601823A (zh) 2018-06-12 2021-04-02 安可济控股有限公司 用于形成连接产物的方法和组合物
CN114045330B (zh) * 2021-12-23 2024-02-09 川北医学院附属医院 一种基于滑动复制的核酸等温扩增方法
CN118621045A (zh) * 2024-08-14 2024-09-10 天健生物制药（天津）有限公司 基于lamp的鸟分枝杆菌检测的组合物和试剂盒

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
ATE437225T1 (de) * 2000-09-19 2009-08-15 Eiken Chemical Verfahren zur polynukleotidsynthese
JPWO2002090538A1 (ja) * 2001-05-08 2004-08-26 栄研化学株式会社 核酸を合成する方法
GB0218215D0 (en) 2002-08-06 2002-09-11 Tepnel Medical Ltd Amplification of nucleic acids
WO2007025281A2 (en) 2005-08-24 2007-03-01 Applera Corporation A method to quantify sirnas, mirnas and polymorphic mirnas
JP4580875B2 (ja) * 2006-01-20 2010-11-17 株式会社東芝 核酸検出法のためのプライマーの設計方法
JP2008029333A (ja) 2006-07-03 2008-02-14 Fujifilm Corp 新規遺伝子増幅法に用いられるプライマー
EP2069540B1 (en) 2006-10-09 2014-03-19 Oxitec Limited Methods for amplifying and detecting nucleic acid sequences
JP4490988B2 (ja) * 2007-04-26 2010-06-30 株式会社東芝 血清アミロイドａ１（ｓａａ１）の遺伝子型を検出するための核酸プライマーセット、キット、及び該プライマーセットを用いた検出方法
US8748100B2 (en) 2007-08-30 2014-06-10 The Chinese University Of Hong Kong Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences

Also Published As

Publication number Publication date
WO2010146349A1 (en) 2010-12-23
CN102652176A (zh) 2012-08-29
JP6106732B2 (ja) 2017-04-05
JP2016041070A (ja) 2016-03-31
AU2010261541B2 (en) 2016-03-10
US9410190B2 (en) 2016-08-09
EP2443253A1 (en) 2012-04-25
CN102652176B (zh) 2016-09-28
CA2765406A1 (en) 2010-12-23
CA2765406C (en) 2019-02-26
AU2010261541A1 (en) 2012-02-02
EP2443253B1 (en) 2016-06-08
JP2012529904A (ja) 2012-11-29
US20120157326A1 (en) 2012-06-21
GB0910302D0 (en) 2009-07-29
ZA201109493B (en) 2015-07-29
ES2589079T3 (es) 2016-11-10

Similar Documents

Publication Publication Date Title
JP6106732B2 (ja) ステム加速等温核酸増幅技術
US12077811B2 (en) Compositions of toehold primer duplexes and methods of use
US7282328B2 (en) Helicase dependent amplification of nucleic acids
CN107849603B (zh) 利用有限核苷酸组成的引物扩增
DK2828399T3 (en) SYSTEM FOR DETECTING POLYMERASE CHAIN REACTION USING OLIGONUCLEOTIDS INCLUDING A PHOSPHOROTHIOATE GROUP
Karami et al. A review of the current isothermal amplification techniques: applications, advantages and disadvantages
JP7175326B2 (ja) 標的核酸増幅方法及び標的核酸増幅用組成物
JP2012514461A (ja) 標的核酸の交差プライミング増幅
CN105899674A (zh) 用于环介导的等温扩增的具有与内部胞嘧啶结合的单个荧光团标记物的核酸探针
JP2017501737A (ja) 核酸と信号プローブの非対称等温増幅を用いた核酸の検出方法
JP7634551B2 (ja) 標的核酸を検出するためのループプライマー及びループ・デ・ループ方法
Lamas et al. Evaluation of the effect of outer primer structure, and inner primer linker sequences, in the performance of Loop-mediated isothermal amplification
ES2925313T3 (es) Método para la detección de secuencias de ácido nucleico diana
JP4297966B2 (ja) 標識ヌクレオチドを利用することによる核酸の新規測定方法
Fischbach et al. Hinge-initiated primer-dependent amplification of nucleic acids (HIP)–a new versatile isothermal amplification method
JPH04229200A (ja) 改良ｌｃｒ法
JP2017538418A (ja) 抗生物質耐性細菌を検出する方法および組成物
CN108026569B (zh) 用于催化性测定的方法和组合物
JP4276897B2 (ja) 標識ヌクレオチドを利用することによる核酸の新規測定方法
Shin Study on biophysical properties of Phi29 DNA polymerase using a novel single-molecule imaging technique
KR20240067091A (ko) 다양한 내부 변형을 갖는 루프형 프라이머 및 표적 탐지를 위한 루프-디-루프 방법
JP2009219445A (ja) Ｖｋｏｒｃ１遺伝子上の１塩基多型を検出する方法およびプライマーセット
Kanu Jain et al. Polymerase Chain Reaction: A Powerful Diagnostic and Research Tool
JP2004033090A (ja) ビブリオ・バルニフィカスの検出方法
HK1188261B (en) Compositions of toehold primer duplexes and methods of use

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130613

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130613

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20140930

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20141225

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150121

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20150609

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151009

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20151216

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20160223

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20160322

R150 Certificate of patent or registration of utility model

Ref document number: 5907864

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees