JP5816100B2 - 多能性細胞の分化 - Google Patents

多能性細胞の分化 Download PDF

Info

Publication number
JP5816100B2
JP5816100B2 JP2011552223A JP2011552223A JP5816100B2 JP 5816100 B2 JP5816100 B2 JP 5816100B2 JP 2011552223 A JP2011552223 A JP 2011552223A JP 2011552223 A JP2011552223 A JP 2011552223A JP 5816100 B2 JP5816100 B2 JP 5816100B2
Authority
JP
Japan
Prior art keywords
cells
differentiation
medium
cell
pluripotent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2011552223A
Other languages
English (en)
Japanese (ja)
Other versions
JP2012519005A (ja
JP2012519005A5 (enExample
Inventor
ディーピカ ラジェシュ，
ディーピカ ラジェシュ，
レイチェル ルイス，
レイチェル ルイス，
Original Assignee
セルラー ダイナミクス インターナショナル， インコーポレイテッド
セルラー ダイナミクス インターナショナル， インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by セルラー ダイナミクス インターナショナル， インコーポレイテッド, セルラー ダイナミクス インターナショナル， インコーポレイテッド filed Critical セルラー ダイナミクス インターナショナル， インコーポレイテッド
Publication of JP2012519005A publication Critical patent/JP2012519005A/ja
Publication of JP2012519005A5 publication Critical patent/JP2012519005A5/ja
Application granted granted Critical
Publication of JP5816100B2 publication Critical patent/JP5816100B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C12N5/0692—Stem cells; Progenitor cells; Precursor cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/14—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/145—Thrombopoietin [TPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/26—Flt-3 ligand (CD135L, flk-2 ligand)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Vascular Medicine (AREA)
  • Diabetes (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2011552223A 2009-02-27 2010-03-01 多能性細胞の分化 Active JP5816100B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15630409P 2009-02-27 2009-02-27
US61/156,304 2009-02-27
PCT/US2010/025776 WO2010099539A1 (en) 2009-02-27 2010-03-01 Differentiation of pluripotent cells

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2015158092A Division JP2015192681A (ja) 2009-02-27 2015-08-10 多能性細胞の分化

Publications (3)

Publication Number Publication Date
JP2012519005A JP2012519005A (ja) 2012-08-23
JP2012519005A5 JP2012519005A5 (enExample) 2013-04-18
JP5816100B2 true JP5816100B2 (ja) 2015-11-18

Family

ID=42097524

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2011552223A Active JP5816100B2 (ja) 2009-02-27 2010-03-01 多能性細胞の分化
JP2015158092A Pending JP2015192681A (ja) 2009-02-27 2015-08-10 多能性細胞の分化

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2015158092A Pending JP2015192681A (ja) 2009-02-27 2015-08-10 多能性細胞の分化

Country Status (9)

Country Link
US (2) US8372642B2 (enExample)
EP (1) EP2401364B1 (enExample)
JP (2) JP5816100B2 (enExample)
KR (1) KR101720961B1 (enExample)
CN (1) CN102388130B (enExample)
AU (1) AU2010217739B2 (enExample)
CA (1) CA2753679C (enExample)
IL (1) IL214818A (enExample)
WO (1) WO2010099539A1 (enExample)

Families Citing this family (140)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2377925A1 (en) 2006-04-14 2011-10-19 Advanced Cell Technology, Inc. Hemangio-colony forming cells
JP5804280B2 (ja) * 2009-09-08 2015-11-04 国立大学法人京都大学 多能性幹細胞からの肥満細胞の製造方法
EP4166652A1 (en) 2009-11-12 2023-04-19 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
US8703487B2 (en) * 2009-12-01 2014-04-22 The Regents Of The University Of California Compositions and methods for making and using bone marrow mesenchymal stem cells and erythroid progenitor cells
US8822218B2 (en) * 2009-12-04 2014-09-02 Stem Cell & Regenerative Medicine International, Inc. Method of generating natural killer cells and dendritic cells from human embryonic stem cell-derived hemangioblasts
US9428735B2 (en) 2010-02-25 2016-08-30 The Johns Hopkins University Smooth muscle-like cells (SMLCs) dervided from human pluripotent stem cells
AU2011267849B2 (en) 2010-06-15 2015-02-05 FUJIFILM Cellular Dynamics, Inc. Generation of induced pluripotent stem cells from small volumes of peripheral blood
WO2012018933A2 (en) 2010-08-04 2012-02-09 Cellular Dynamics International, Inc. Reprogramming immortalized b cells
CN106119200A (zh) 2010-09-10 2016-11-16 国立大学法人东京大学 涉及由多能干细胞向血细胞分化的培养方法
WO2012103098A2 (en) * 2011-01-24 2012-08-02 Immunepath, Inc. Compositions and methods for treating hematological cytopenias
JP6148429B2 (ja) * 2011-01-31 2017-06-14 協和発酵バイオ株式会社 ヒト多能性幹細胞の培養方法
WO2012109208A2 (en) * 2011-02-08 2012-08-16 Cellular Dynamics International, Inc. Hematopoietic precursor cell production by programming
CN102161980B (zh) * 2011-02-12 2013-07-31 浙江大学 用人间充质干细胞为滋养层培养诱导多能干细胞的方法
WO2012115276A1 (en) 2011-02-23 2012-08-30 Kyoto University Method for producing dendritic cells from pluripotent stem cells
GB201103600D0 (en) * 2011-03-01 2011-04-13 Isis Innovation Dendritic cells
US9169309B2 (en) * 2011-03-01 2015-10-27 Humanzyme Inc. Thermostable variants of fibroblast growth factors
WO2012135621A2 (en) 2011-03-30 2012-10-04 Cellular Dynamics International. Inc Priming of pluripotent stem cells for neural differentiation
JP6124880B2 (ja) * 2011-06-09 2017-05-10 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 多能性幹細胞の血管床細胞への分化のための方法
AU2012315783B2 (en) * 2011-09-29 2015-08-20 Cellsnap, Llc Compositions and methods for toxigenicity testing
KR102031386B1 (ko) * 2011-10-03 2019-10-11 닛산 가가쿠 가부시키가이샤 다능성 간세포로부터의 거핵구 및/또는 혈소판의 제조 방법
CN104053769A (zh) * 2011-11-21 2014-09-17 大学健康网络 造血祖细胞群体和富集其干细胞的方法
TW202537630A (zh) 2011-11-30 2025-10-01 美商安斯泰來再生醫藥協會 利用血管母細胞產生間葉基質細胞之方法
US8961956B2 (en) 2011-11-30 2015-02-24 Ocata Therapeutics, Inc. Mesenchymal stromal cells and uses related thereto
US10428309B2 (en) 2011-12-01 2019-10-01 New York Stem Cell Foundation, Inc. Systems and methods for producing stem cells and differentiated cells
JP6257520B2 (ja) * 2011-12-01 2018-01-10 ニューヨーク ステム セル ファウンデーション インコーポレイテッド 人工多能性幹細胞または分化細胞を作製するための自動化されたシステム
EP2820123B1 (en) * 2012-02-29 2017-10-11 The Johns Hopkins University Method for guiding the derivation of endothelial cells from human pluripotent stem cells employing two-dimensional, feeder-free differentiation
EP2841563B1 (en) 2012-04-24 2019-06-12 Dan S. Kaufman Method for developing natural killer cells from stem cells
GB201210857D0 (en) 2012-06-19 2012-08-01 Cambridge Entpr Ltd Transcription factor mediated programming towards megakaryocytes
CN104471059B (zh) 2012-07-12 2018-04-17 珠海横琴爱姆斯坦生物科技有限公司 人胚胎干细胞衍生的间充质样干细胞、方法及其应用
WO2014051154A1 (ja) * 2012-09-28 2014-04-03 公益財団法人先端医療振興財団 虚血性疾患治療に適した細胞を含む細胞群の生体外増幅方法
RU2015123810A (ru) 2012-11-21 2017-01-10 Мерц Фарма Гмбх Унд Ко. Кгаа Средства и способы определения биологической активности ботулинического нейротоксина
CA3177929A1 (en) 2012-12-21 2014-06-26 Astellas Institute For Regenerative Medicine Methods for production of platelets from pluripotent stem cells and compositions thereof
US9938499B2 (en) * 2013-03-13 2018-04-10 Wisconsin Alumni Research Foundation Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions
US9506037B2 (en) 2013-03-15 2016-11-29 The Johns Hopkins University Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix
EP2970902B1 (en) * 2013-03-15 2019-11-20 The Johns Hopkins University Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix
JP2016509863A (ja) 2013-03-15 2016-04-04 ラーソン、マルクス カーレ、トールレイフＬＡＲＳＳＯＮ， Ｍａｒｃｕｓ Ｋａｒｅ， Ｔｏｒｌｅｉｆ 細胞、臍帯血収集のための方法及び装置、並びに細胞の単離のための方法及び装置
US9994825B2 (en) 2013-03-15 2018-06-12 The Johns Hopkins University Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix
US9822342B2 (en) 2013-05-14 2017-11-21 Kyoto University Method of efficiently inducing cardiomyocytes
JP5511039B1 (ja) * 2013-05-22 2014-06-04 国立大学法人九州大学 Ｎｋ細胞の調製方法
WO2014189144A1 (ja) * 2013-05-23 2014-11-27 国立大学法人京都大学 骨髄異形成症候群等の治療／予防薬のスクリーニング方法
US10119122B2 (en) * 2013-06-10 2018-11-06 Academisch Ziekenhuis Leiden Differentiation and expansion of endothelial cells from pluripotent stem cells and the in vitro formation of vasculature like structures
JP6608357B2 (ja) 2013-06-28 2019-11-20 メルツ ファルマ ゲーエムベーハー ウント コンパニー カーゲーアーアー 細胞において神経毒ポリペプチドの生物活性を決定する手段及び方法
CA2925774A1 (en) 2013-10-01 2015-04-09 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Human ipsc-derived vascular-related and hematopoetic cells for therapies and toxicology/drug screenings
WO2015119642A1 (en) * 2014-02-10 2015-08-13 The Johns Hopkins University Low oxygen tension enhances endothelial fate of human pluripotent stem cells
CN106133522B (zh) 2014-02-19 2018-10-12 莫茨药物股份两合公司 在体外试验系统中用于使细胞对肉毒神经毒素的敏感性标准化和增加的神经节苷脂
CA2945393C (en) 2014-04-24 2021-03-23 Board Of Regents, The University Of Texas System Application of induced pluripotent stem cells to generate adoptive cell therapy products
CN103937743B (zh) * 2014-04-27 2017-12-01 浙江大学 一种利用三维诱导系统获得造血干细胞的方法
LT3177612T (lt) 2014-08-04 2022-06-10 Nuevolution A/S Pirimidino dariniai, pakeisti pasirinktinai kondensuotu heterociklilu, tinkami uždegiminių, metabolinių, onkologinių ir autoimuninių ligų gydymui
CN107207586B (zh) 2014-12-19 2021-07-13 莫茨药物股份两合公司 用于测定BoNT/E在细胞中的生物活性的装置和方法
WO2016114723A1 (en) 2015-01-16 2016-07-21 Agency For Science, Technology And Research Differentiation of macrophages from pluripotent stem cells
WO2016118824A1 (en) 2015-01-22 2016-07-28 Regenerative Medical Solutions, Inc. Markers for differentiation of stem cells into differentiated cell populations
EP3250680A4 (en) * 2015-01-26 2018-12-05 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
TWI704156B (zh) 2015-03-04 2020-09-11 德商曼茲法瑪股份有限公司 用於增進肉毒桿菌神經毒素進入細胞之特定攝取的方法
US11680244B2 (en) 2015-05-20 2023-06-20 The Regents Of The University Of California Method for generating human dendritic cells for immunotherapy
EP3310388B1 (en) * 2015-06-18 2021-10-06 Yeda Research and Development Co., Ltd. Conditioning protocols and use of same for tissue regeneration
WO2017043604A1 (ja) 2015-09-08 2017-03-16 大日本住友製薬株式会社 網膜組織の製造方法
CA3002157A1 (en) 2015-10-20 2017-04-27 FUJIFILM Cellular Dynamics, Inc. Methods for directed differentiation of pluripotent stem cells to immune cells
US11154573B2 (en) 2015-10-30 2021-10-26 The Regents Of The University Of California Methods of generating T-cells from stem cells and immunotherapeutic methods using the T-cells
AU2016349504B2 (en) 2015-11-04 2023-02-09 Fate Therapeutics, Inc. Genomic engineering of pluripotent cells
SG11201803145RA (en) 2015-11-04 2018-05-30 Fate Therapeutics Inc Methods and compositions for inducing hematopoietic cell differentiation
CN105296428A (zh) * 2015-12-03 2016-02-03 广州医科大学附属第三医院 一种提高诱导多能干细胞的体外分化造血效率的方法
CN108699518A (zh) * 2015-12-29 2018-10-23 株式会社可乐丽 干细胞聚集体的群体的制备方法
GB201601503D0 (en) 2016-01-27 2016-03-09 Isis Innovation Dendritic cells
CN109415680B (zh) * 2016-05-18 2022-08-16 学校法人庆应义塾 类器官培养用细胞培养基、培养方法及类器官
EP3492577A4 (en) * 2016-07-29 2020-04-08 Daikin Industries, Ltd. METHOD FOR PRODUCING DIFFERENTIATED CELLS AND CULTURE POCKET USED THEREFOR
KR102288953B1 (ko) * 2016-09-02 2021-08-11 다카라 바이오 가부시키가이샤 다능성 줄기세포로부터 마이크로글리아를 얻는 방법
JP7215994B2 (ja) 2016-09-06 2023-01-31 ザ チルドレンズ メディカル センター コーポレーション 人工多能性幹細胞由来の免疫細胞
DK3523423T3 (da) * 2016-10-05 2024-09-02 Fujifilm Cellular Dynamics Inc Fremgangsmåder til målrettet differentiering af pluripotente stamceller til hla homozygote immunceller
AU2017340634B2 (en) * 2016-10-05 2022-06-02 FUJIFILM Cellular Dynamics, Inc. Generating mature lineages from induced pluripotent stem cells with MECP2 disruption
CN106479968A (zh) * 2016-10-11 2017-03-08 海南博鳌龄迈迪精准医学研究院有限公司 脐带间充质干细胞的培养方法
WO2018073615A1 (en) 2016-10-21 2018-04-26 Longboat Explorers Ab Methods and compositions for generating hematopoietic cells
CN106754684A (zh) * 2017-01-05 2017-05-31 厚朴生物科技（苏州）有限公司 一种睾丸间质干细胞分离培养分化方法
WO2018139600A1 (ja) 2017-01-27 2018-08-02 株式会社カネカ 内胚葉系細胞集団、及び多能性細胞から三胚葉のいずれかの細胞集団を製造する方法
JP7265988B2 (ja) * 2017-02-03 2023-04-27 コーネル ユニヴァーシティー 安定な３次元血管およびそれを形成するための方法
CA3056709A1 (en) * 2017-03-21 2018-09-27 Etablissement Francais Du Sang Methods of improving hematopoietic grafts
ES2912383T3 (es) * 2017-04-18 2022-05-25 Fujifilm Cellular Dynamics Inc Células efectoras inmunitarias específicas de antígeno
EP3399027A1 (en) 2017-05-04 2018-11-07 Medizinische Hochschule Hannover Stem-cell derived myeloid cells, generation and use thereof
CN107083367B (zh) * 2017-07-03 2020-07-14 广州润虹医药科技股份有限公司 培养基及其用途与由尿液细胞制备间充质干细胞的方法
WO2019117444A1 (ko) * 2017-12-11 2019-06-20 대한민국(관리부서 질병관리본부장) 전분화능 줄기세포를 이용한 면역세포 분화
WO2019126146A1 (en) 2017-12-19 2019-06-27 Xcell Biosciences, Inc. Methods of modulating cell phenotype by way of regulating the gaseous environment
CN108048390B (zh) * 2017-12-21 2021-04-27 清华大学 一种制备血管内皮细胞的方法及其专用试剂盒
CN108588024B (zh) * 2017-12-29 2020-04-21 广州医科大学附属第三医院（广州重症孕产妇救治中心、广州柔济医院） 诱导多能干细胞分化成造血干细胞的培养基及方法
AU2019205942B2 (en) * 2018-01-05 2025-05-15 Platelet Biogenesis, Inc. Compositions and methods for producing megakaryocytes
JP7268039B2 (ja) 2018-02-01 2023-05-02 エヌケーマックス カンパニー リミテッド がん治療のためのナチュラルキラー細胞および組成物の製造方法
FR3079239A1 (fr) * 2018-03-23 2019-09-27 Centre National De La Recherche Scientifique Nouvelle methode d’obtention de cellules t a partir de cellules souches pluripotentes, et leurs utilisations
US12403161B2 (en) 2018-06-29 2025-09-02 Stellular Bio, Inc. Compositions for drug delivery and methods of use thereof
CA3110706A1 (en) 2018-09-19 2020-03-26 FUJIFILM Cellular Dynamics, Inc. Protein l for activation and expansion of chimeric antigen receptor-modified immune cells
CN109266618B (zh) * 2018-10-18 2021-04-23 赛元生物科技(杭州)有限公司 能够靶向肿瘤细胞的巨噬细胞及其制备方法
WO2020086889A1 (en) * 2018-10-24 2020-04-30 Hebecell Corporation Methods and systems for manufacturing hematopoietic lineage cells
KR102111441B1 (ko) * 2018-11-12 2020-05-15 대한민국 전분화능 줄기세포 유래 대식세포를 기반으로 하는 감염질환 치료용 약물 스크리닝 방법
EP3901253A4 (en) * 2018-12-20 2022-09-07 Sumitomo Chemical Company Limited EMBRYONIC ERYTHROBLAST CONTAINING CELL POPULATION AND METHODS FOR THEIR PRODUCTION, CELL CULTURE COMPOSITION AND COMBINATION TESTING METHODS
JP7634938B2 (ja) * 2019-02-21 2025-02-25 ピービーエス バイオテック インコーポレイテッド バイオリアクターにおける治療細胞の膨張および分化のためのシステムおよび方法
CN111206017B (zh) * 2019-04-30 2022-02-18 浙江大学 一种干细胞无血清培养基及其应用
CN114174493B (zh) * 2019-06-14 2025-10-28 富士胶片细胞动力公司 使用带电表面从诱导多能干细胞产生多个谱系的方法
US12465878B2 (en) 2019-06-20 2025-11-11 Amniotics Ab Apparatus for filtering amniotic fluid
EP4045068A1 (en) 2019-10-18 2022-08-24 Amniotics AB Use of term amniotic fluid cells for the treatment of acute and chronic respiratory diseases
EP4060024A4 (en) 2019-11-13 2023-12-06 Karis Bio Inc. METHOD FOR ISOLATING PURE CULTURE OF VASCULAR ENDOTHELIAL CELLS, MEDIUM FOR MAINTAINING THE CHARACTERISTICS OF VASCULAR ENDOTHELIAL CELLS, AND CULTURE METHOD COMPRISING SAME
CN110938584B (zh) * 2019-11-25 2021-12-03 广州赛莱拉干细胞科技股份有限公司 一种人胚胎干细胞向内皮细胞诱导分化的方法
MX2022007265A (es) 2019-12-20 2022-09-09 Nuevolution As Compuestos activos frente a receptores nucleares.
TWI872177B (zh) 2019-12-20 2025-02-11 丹麥商紐韋盧森公司 對核受體具有活性之化合物
JP2023515579A (ja) * 2020-02-28 2023-04-13 パーデュー・リサーチ・ファウンデーション 規定の条件下でのヒト多能性幹細胞からの大動脈－生殖腺－中腎様造血細胞の生成
US12220445B2 (en) 2020-03-09 2025-02-11 Amprion, Inc. Inert matrices for qualitative and semi-quantitative seed amplification assays
CN111440760B (zh) * 2020-03-09 2022-09-23 浙江大学 一种人多能干细胞高效分化得到内皮祖细胞的方法
US11547726B2 (en) 2020-03-26 2023-01-10 Honed Life Sciences, Llc Enhancement of production of NK cells from stem cells
US11613532B2 (en) 2020-03-31 2023-03-28 Nuevolution A/S Compounds active towards nuclear receptors
US11780843B2 (en) 2020-03-31 2023-10-10 Nuevolution A/S Compounds active towards nuclear receptors
JP2023526670A (ja) 2020-05-22 2023-06-22 インシトロ インコーポレイテッド 機械学習モデルを使用した疾患の転帰の予測
US20230257705A1 (en) 2020-06-17 2023-08-17 Kyoto University Chimeric antigen receptor-expressing immunocompetent cells
AU2021317752A1 (en) * 2020-07-27 2023-03-02 Stemcell Technologies Canada Inc. Systems and methods for differentiating hematopoietic cells
CN111808812B (zh) * 2020-08-31 2020-12-15 首都医科大学附属北京友谊医院 一种用于多能干细胞向造血干细胞分化的材料和方法
US12435308B2 (en) 2020-11-06 2025-10-07 Amniotics Ab Immunomodulation by amniotic fluid mesenchymal stem cells
WO2022113056A1 (en) 2020-11-30 2022-06-02 Crispr Therapeutics Ag Gene-edited natural killer cells
US11661459B2 (en) 2020-12-03 2023-05-30 Century Therapeutics, Inc. Artificial cell death polypeptide for chimeric antigen receptor and uses thereof
TW202237827A (zh) 2020-12-03 2022-10-01 美商世紀治療股份有限公司 經基因工程改造之細胞及其用途
CN112608895B (zh) * 2020-12-18 2024-02-27 深圳市济因生物科技有限公司 一种人多能干细胞分化的自然杀伤细胞及其制备方法与应用
EP4271798A1 (en) 2020-12-30 2023-11-08 CRISPR Therapeutics AG Compositions and methods for differentiating stem cells into nk cells
JP7729901B2 (ja) * 2021-02-05 2025-08-26 スンクワン メディカル ファウンデーション 多能性幹細胞由来の造血性内皮細胞からのリンパ球系細胞の分化方法
CA3210702A1 (en) 2021-04-07 2022-10-13 Century Therapeutics, Inc. Gene transfer vectors and methods of engineering cells
CA3214473A1 (en) 2021-04-07 2022-10-13 Century Therapeutics, Inc. Compositions and methods for generating alpha-beta t cells from induced pluripotent stem cells
CA3214661A1 (en) 2021-04-07 2022-10-13 Century Therapeutics, Inc. Combined artificial cell death/reporter system polypeptide for chimeric antigen receptor cell and uses thereof
IL307358A (en) 2021-04-07 2023-11-01 Century Therapeutics Inc Compositions and methods for generating gamma-delta t cells from induced pluripotent stem cells
EP4334438A1 (en) 2021-05-05 2024-03-13 Fujifilm Cellular Dynamics, Inc. Methods and compositions for ipsc-derived microglia
EP4399282A2 (en) 2021-09-10 2024-07-17 Fujifilm Cellular Dynamics, Inc. Compositions of induced pluripotent stem cell-derived cells and methods of use thereof
CA3232289A1 (en) * 2021-09-13 2023-03-16 Amprion, Inc. Inert matrices for qualitative and semi-quantitative seed amplification assays
US20250051478A1 (en) 2021-12-15 2025-02-13 Y-Mabs Therapeutics, Inc. scFv and Antibodies with Reduced Multimerisation
WO2023129937A1 (en) 2021-12-29 2023-07-06 Century Therapeutics, Inc. Genetically engineered cells having anti-cd19 / anti-cd22 chimeric antigen receptors, and uses thereof
WO2023164440A1 (en) 2022-02-22 2023-08-31 Juno Therapeutics, Inc. Proteinase 3 (pr3) chimeric autoantibody receptor t cells and related methods and uses
CN119365492A (zh) 2022-05-04 2025-01-24 世纪治疗股份有限公司 用hla-e和hla-g转基因工程化的细胞
WO2023240212A2 (en) 2022-06-08 2023-12-14 Century Therapeutics, Inc. Genetically engineered cells having anti-cd133 / anti-egfr chimeric antigen receptors, and uses thereof
CN119698467A (zh) 2022-06-08 2025-03-25 世纪治疗股份有限公司 衍生自遗传工程化以具有膜结合的il12的诱导多能干细胞的免疫效应细胞及其用途
AU2023284442A1 (en) 2022-06-08 2024-12-05 Century Therapeutics, Inc. Genetically engineered cells expressing cd16 variants and nkg2d and uses thereof
WO2024102838A1 (en) 2022-11-09 2024-05-16 Century Therapeutics, Inc. Engineered interleukin-7 receptors and uses thereof
IL320650A (en) 2022-11-10 2025-07-01 Century Therapeutics Inc Genetically modified cells with chimeric anti-nectin 4 antigen receptors and uses thereof
WO2024137677A1 (en) 2022-12-19 2024-06-27 FUJIFILM Holdings America Corporation Extracellular vesicle-enriched secretome composition derived from induced pluripotent stem cell derived-microglia and methods of use thereof
KR20250160941A (ko) * 2023-02-16 2025-11-14 신택스 바이오, 인크. 조혈 계통으로의 세포 프로그래밍을 위한 시스템 및 이의 방법
CN116426472A (zh) * 2023-06-08 2023-07-14 呈诺再生医学科技(北京)有限公司 一种促进造血干细胞或造血祖细胞分化为红细胞的诱导分化体系及其应用
WO2025101938A2 (en) 2023-11-10 2025-05-15 Century Therapeutics, Inc. Genetically engineered cells having multi-transmembrane domain chimeric antigen receptors utilizing g protein-coupled receptor scaffolds, and uses thereof
WO2025106626A1 (en) 2023-11-15 2025-05-22 Century Therapeutics, Inc. Genetically engineered cells expressing c-x-c chemokine receptor type 4, and uses thereof
WO2025207798A1 (en) 2024-03-26 2025-10-02 Century Therapeutics, Inc. Genetically engineered cells having anti-cd123 chimeric antigen receptors, and uses thereof
WO2025207795A1 (en) 2024-03-26 2025-10-02 Juno Therapeutics, Inc. Genetically engineered cells having anti-cd33 / anti-cd123 chimeric antigen receptors, and uses thereof

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0187371B1 (en) 1984-12-27 1991-06-19 Asahi Kasei Kogyo Kabushiki Kaisha Substituted isoquinolinesulfonyl compounds
JP2000515525A (ja) 1996-07-19 2000-11-21 アベーグ，グンナー 尿と胃腸の疾患の治療におけるｓ（―）―トルテロジン
AU5734998A (en) 1997-01-10 1998-08-03 Life Technologies, Inc. Embryonic stem cell serum replacement
JP4531990B2 (ja) 1999-03-25 2010-08-25 田辺三菱製薬株式会社 間質性肺炎・肺線維症の予防・治療薬
AU5250400A (en) 1999-06-18 2001-01-09 Mitsubishi Pharma Corporation Osteogenesis promoters
EP1367899A4 (en) * 2001-02-14 2004-07-28 Leo T Furcht TOTIPOTENT ADULT STEM CELLS, SOURCES OF SUCH CELLS, METHODS FOR OBTAINING AND MAINTAINING SAME, METHODS FOR DIFFERENTIATING THESE CELLS, METHODS OF USING SAME, AND CELLS DERIVED FROM THE ABOVE-MENTIONED CELLS
GB2412379B (en) * 2001-12-07 2006-03-29 Geron Corp Hematopoietic cells from human embryonic stem cells
IL162648A0 (en) * 2001-12-21 2005-11-20 Mount Sinai Hospital Corp Cellular compositions and methods of making and using them
MXPA04007196A (es) * 2002-01-23 2005-06-08 Bayer Pharmaceuticals Corp Inhibidores de rho-quinasa.
US6664266B2 (en) * 2002-03-14 2003-12-16 Children's Medical Center Corporation Axon regeneration with PKC inhibitiors
GB0206860D0 (en) 2002-03-22 2002-05-01 Glaxo Group Ltd Compounds
WO2003089456A2 (en) * 2002-04-22 2003-10-30 The Board Of Trustees Of The Leland Stanford Junior University Peptide inhibitors of protein kinase c
US7723106B2 (en) * 2004-07-29 2010-05-25 Food Industry Research & Development Institute Stroma-free, serum-free, and chemically defined medium and method for ex vivo mononuclear cell expansion using the same
BRPI0508594A (pt) 2004-03-08 2007-08-21 Wyeth Corp moduladores de canal de ìon
US7452718B2 (en) * 2004-03-26 2008-11-18 Geron Corporation Direct differentiation method for making cardiomyocytes from human embryonic stem cells
CN101052712A (zh) * 2004-09-08 2007-10-10 威斯康星校友研究基金会 培养人胚胎干细胞
AU2005302258B2 (en) 2004-11-01 2010-10-21 Wisconsin Alumni Research Foundation Platelets from stem cells
US20070077654A1 (en) * 2004-11-01 2007-04-05 Thomson James A Platelets from stem cells
WO2006090882A1 (ja) * 2005-02-23 2006-08-31 Foundation For Biomedical Research And Innovation 血管内皮前駆細胞の生体外増幅方法
EP1960777B1 (en) * 2005-11-28 2009-10-21 Choongwae Pharma Corporation Serum-free expansion of cells in culture
JP2008099662A (ja) * 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
JP5067949B2 (ja) * 2006-11-09 2012-11-07 独立行政法人国立国際医療研究センター 霊長類動物胚性幹細胞の培養及び継代方法、並びにその分化誘導方法
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
KR101689124B1 (ko) 2008-03-27 2016-12-23 아스테리아스 바이오세라퓨틱스, 인크. 영장류 다능성 줄기 세포의 조혈계 세포로의 분화
WO2009135206A1 (en) 2008-05-02 2009-11-05 Stem Cell Products, Inc. Method for production of mast cells from stem cells
WO2010035136A2 (en) * 2008-09-25 2010-04-01 Uti Limited Partnership Expansion of embryonic stem cells

Also Published As

Publication number Publication date
IL214818A (en) 2014-07-31
JP2015192681A (ja) 2015-11-05
CA2753679C (en) 2021-03-02
KR101720961B1 (ko) 2017-03-29
US8372642B2 (en) 2013-02-12
CA2753679A1 (en) 2010-09-02
CN102388130A (zh) 2012-03-21
AU2010217739B2 (en) 2015-09-03
US10100282B2 (en) 2018-10-16
JP2012519005A (ja) 2012-08-23
WO2010099539A1 (en) 2010-09-02
KR20110122858A (ko) 2011-11-11
IL214818A0 (en) 2011-11-30
US20130210141A1 (en) 2013-08-15
CN102388130B (zh) 2014-08-27
US20100279403A1 (en) 2010-11-04
AU2010217739A1 (en) 2011-09-08
EP2401364A1 (en) 2012-01-04
EP2401364B1 (en) 2015-04-22

Similar Documents

Publication Publication Date Title
JP5816100B2 (ja) 多能性細胞の分化
JP2012519005A5 (enExample)
AU2020264375B2 (en) Method for developing natural killer cells from stem cells
JP6189581B2 (ja) 幹細胞の分化のための方法および組成物
US20200157503A1 (en) Hemangio colony forming cells and non-engrafting hemangio cells
CN117264888A (zh) 一种制备造血干细胞和祖细胞的方法
HK40017506A (en) Method for developing natural killer cells from stem cells

Legal Events

Date Code Title Description
A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20130228

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130228

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20140710

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20140911

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20140919

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20141128

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150420

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20150715

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150810

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20150903

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20150925

R150 Certificate of patent or registration of utility model

Ref document number: 5816100

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: R3D03

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250