CA2753679C - Differentiation of pluripotent cells - Google Patents
Differentiation of pluripotent cells Download PDFInfo
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- CA2753679C CA2753679C CA2753679A CA2753679A CA2753679C CA 2753679 C CA2753679 C CA 2753679C CA 2753679 A CA2753679 A CA 2753679A CA 2753679 A CA2753679 A CA 2753679A CA 2753679 C CA2753679 C CA 2753679C
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- cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/069—Vascular Endothelial cells
- C12N5/0692—Stem cells; Progenitor cells; Precursor cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/02—Atmosphere, e.g. low oxygen conditions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/14—Erythropoietin [EPO]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/145—Thrombopoietin [TPO]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/165—Vascular endothelial growth factor [VEGF]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/26—Flt-3 ligand (CD135L, flk-2 ligand)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Vascular Medicine (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Diabetes (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15630409P | 2009-02-27 | 2009-02-27 | |
| US61/156,304 | 2009-02-27 | ||
| PCT/US2010/025776 WO2010099539A1 (en) | 2009-02-27 | 2010-03-01 | Differentiation of pluripotent cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2753679A1 CA2753679A1 (en) | 2010-09-02 |
| CA2753679C true CA2753679C (en) | 2021-03-02 |
Family
ID=42097524
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2753679A Active CA2753679C (en) | 2009-02-27 | 2010-03-01 | Differentiation of pluripotent cells |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US8372642B2 (enExample) |
| EP (1) | EP2401364B1 (enExample) |
| JP (2) | JP5816100B2 (enExample) |
| KR (1) | KR101720961B1 (enExample) |
| CN (1) | CN102388130B (enExample) |
| AU (1) | AU2010217739B2 (enExample) |
| CA (1) | CA2753679C (enExample) |
| IL (1) | IL214818A (enExample) |
| WO (1) | WO2010099539A1 (enExample) |
Families Citing this family (140)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102651433B1 (ko) | 2006-04-14 | 2024-03-25 | 아스텔라스 인스티튜트 포 리제너러티브 메디슨 | 혈관 콜로니 형성 세포 |
| EP2475767B1 (en) * | 2009-09-08 | 2017-04-19 | Kyoto University | Method for producing mast cells from pluripotent stem cells |
| US9018010B2 (en) | 2009-11-12 | 2015-04-28 | Technion Research & Development Foundation Limited | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
| US8703487B2 (en) * | 2009-12-01 | 2014-04-22 | The Regents Of The University Of California | Compositions and methods for making and using bone marrow mesenchymal stem cells and erythroid progenitor cells |
| BR112012014104A2 (pt) * | 2009-12-04 | 2016-07-05 | Stem Cell & Regenerative Medicine International Inc | método de geração de células destrutoras naturais e células dendríticas de hemangioblastos embrionários humanos derivados de células-tronco |
| US9428735B2 (en) | 2010-02-25 | 2016-08-30 | The Johns Hopkins University | Smooth muscle-like cells (SMLCs) dervided from human pluripotent stem cells |
| WO2011159684A2 (en) | 2010-06-15 | 2011-12-22 | Cellular Dynamics International, Inc. | Generation of induced pluripotent stem cells from small volumes of peripheral blood |
| US8765470B2 (en) | 2010-08-04 | 2014-07-01 | Cellular Dynamics International, Inc. | Reprogramming immortalized B-cells to induced pluripotent stem cells |
| EP2615165B1 (en) | 2010-09-10 | 2016-08-03 | The University of Tokyo | Culture method related to differentiation of pluripotent stem cells into blood cells |
| WO2012103098A2 (en) * | 2011-01-24 | 2012-08-02 | Immunepath, Inc. | Compositions and methods for treating hematological cytopenias |
| JP6148429B2 (ja) * | 2011-01-31 | 2017-06-14 | 協和発酵バイオ株式会社 | ヒト多能性幹細胞の培養方法 |
| WO2012109208A2 (en) * | 2011-02-08 | 2012-08-16 | Cellular Dynamics International, Inc. | Hematopoietic precursor cell production by programming |
| CN102161980B (zh) * | 2011-02-12 | 2013-07-31 | 浙江大学 | 用人间充质干细胞为滋养层培养诱导多能干细胞的方法 |
| US9499789B2 (en) | 2011-02-23 | 2016-11-22 | Kyoto University | Method for producing dendritic cells from pluripotent stem cells |
| GB201103600D0 (en) * | 2011-03-01 | 2011-04-13 | Isis Innovation | Dendritic cells |
| US9169309B2 (en) * | 2011-03-01 | 2015-10-27 | Humanzyme Inc. | Thermostable variants of fibroblast growth factors |
| WO2012135621A2 (en) | 2011-03-30 | 2012-10-04 | Cellular Dynamics International. Inc | Priming of pluripotent stem cells for neural differentiation |
| WO2012168167A1 (en) * | 2011-06-09 | 2012-12-13 | F. Hoffmann-La Roche Ag | Method for differentiation of pluripotent stem cells into vascular bed cells |
| KR101640694B1 (ko) * | 2011-09-29 | 2016-07-18 | 셀스냅, 엘엘씨 | 독소생산능 시험용 조성물 및 방법 |
| EP2765187B1 (en) * | 2011-10-03 | 2018-08-08 | Nissan Chemical Industries, Ltd. | Method for producing megakaryocytes and/or platelets from pluripotent stem cells |
| WO2013075222A1 (en) * | 2011-11-21 | 2013-05-30 | University Health Network | Populations of hematopoietic progenitors and methods of enriching stem cells therefor |
| US8961956B2 (en) | 2011-11-30 | 2015-02-24 | Ocata Therapeutics, Inc. | Mesenchymal stromal cells and uses related thereto |
| RS57886B1 (sr) | 2011-11-30 | 2019-01-31 | Astellas Inst For Regenerative Medicine | Mezenhimalne stromalne ćelije i primene vezane za njih |
| US10428309B2 (en) | 2011-12-01 | 2019-10-01 | New York Stem Cell Foundation, Inc. | Systems and methods for producing stem cells and differentiated cells |
| AU2012345676B2 (en) | 2011-12-01 | 2018-10-04 | New York Stem Cell Foundation, Inc. | Automated system for producing induced pluripotent stem cells or differentiated cells |
| WO2013130820A1 (en) * | 2012-02-29 | 2013-09-06 | The Johns Hopkins University | Method for guiding the derivation of endothelial cells from human pluripotent stem cells employing two-dimensional, feeder-free differentiation |
| US9260696B2 (en) | 2012-04-24 | 2016-02-16 | Dan S. Kaufman | Method for developing natural killer cells from stem cells |
| GB201210857D0 (en) * | 2012-06-19 | 2012-08-01 | Cambridge Entpr Ltd | Transcription factor mediated programming towards megakaryocytes |
| CA2876499C (en) | 2012-07-12 | 2021-10-26 | Imstem Biotechnology, Inc. | Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof |
| CN105073980A (zh) * | 2012-09-28 | 2015-11-18 | 公益财团法人先端医疗振兴财团 | 含有适合用于缺血性疾病治疗的细胞的细胞群体的体外增殖方法 |
| US20160289731A1 (en) | 2012-11-21 | 2016-10-06 | Merz Pharma Gmbh & Co, Kgaa | Means and methods for determination of botulinum neurotoxin biological activity |
| CN114558032B (zh) * | 2012-12-21 | 2025-07-29 | 安斯泰来再生医药协会 | 由多能干细胞制备血小板的方法及其组合物 |
| SG11201507275TA (en) * | 2013-03-13 | 2015-10-29 | Wisconsin Alumni Res Found | Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions |
| JP2016509863A (ja) | 2013-03-15 | 2016-04-04 | ラーソン、マルクス カーレ、トールレイフLARSSON, Marcus Kare, Torleif | 細胞、臍帯血収集のための方法及び装置、並びに細胞の単離のための方法及び装置 |
| US9506037B2 (en) | 2013-03-15 | 2016-11-29 | The Johns Hopkins University | Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix |
| CA2906188A1 (en) * | 2013-03-15 | 2014-09-18 | The Johns Hopkins University | Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix |
| US9994825B2 (en) | 2013-03-15 | 2018-06-12 | The Johns Hopkins University | Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix |
| JP6429280B2 (ja) * | 2013-05-14 | 2018-11-28 | 国立大学法人京都大学 | 効率的な心筋細胞の誘導方法 |
| JP5511039B1 (ja) * | 2013-05-22 | 2014-06-04 | 国立大学法人九州大学 | Nk細胞の調製方法 |
| JP6436491B2 (ja) * | 2013-05-23 | 2018-12-12 | 国立大学法人京都大学 | 骨髄異形成症候群等の治療/予防薬のスクリーニング方法 |
| EP3008169B1 (en) * | 2013-06-10 | 2021-11-10 | Academisch Ziekenhuis Leiden | Differentiation and expansion of endothelial cells from pluripotent stem cells and the in vitro formation of vasculature like structures |
| JP6608357B2 (ja) | 2013-06-28 | 2019-11-20 | メルツ ファルマ ゲーエムベーハー ウント コンパニー カーゲーアーアー | 細胞において神経毒ポリペプチドの生物活性を決定する手段及び方法 |
| WO2015050963A1 (en) * | 2013-10-01 | 2015-04-09 | Manfred Boehm | Human ipsc-derived vascular-related and hematopoetic cells for therapies and toxicology/drug screenings |
| WO2015119642A1 (en) * | 2014-02-10 | 2015-08-13 | The Johns Hopkins University | Low oxygen tension enhances endothelial fate of human pluripotent stem cells |
| AU2015220915B2 (en) | 2014-02-19 | 2020-09-17 | Merz Pharma Gmbh & Co. Kgaa | Gangliosides for standardizing and increasing the sensitivity of cells to Botulinum neurotoxins in in vitro test systems |
| WO2015164740A1 (en) | 2014-04-24 | 2015-10-29 | Board Of Regents, The University Of Texas System | Application of induced pluripotent stem cells to generate adoptive cell therapy products |
| CN103937743B (zh) * | 2014-04-27 | 2017-12-01 | 浙江大学 | 一种利用三维诱导系统获得造血干细胞的方法 |
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| CA2998091C (en) * | 2015-09-08 | 2024-06-11 | Sumitomo Dainippon Pharma Co., Ltd. | Method for producing retinal tissue |
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