JP5639654B2 - オンチップでの非干渉性のレンズフリーのホログラフィおよび顕微鏡法 - Google Patents
オンチップでの非干渉性のレンズフリーのホログラフィおよび顕微鏡法 Download PDFInfo
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- JP5639654B2 JP5639654B2 JP2012535307A JP2012535307A JP5639654B2 JP 5639654 B2 JP5639654 B2 JP 5639654B2 JP 2012535307 A JP2012535307 A JP 2012535307A JP 2012535307 A JP2012535307 A JP 2012535307A JP 5639654 B2 JP5639654 B2 JP 5639654B2
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- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H2222/00—Light sources or light beam properties
- G03H2222/20—Coherence of the light source
- G03H2222/24—Low coherence light normally not allowing valuable record or reconstruction
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- G—PHYSICS
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- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
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- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H2240/00—Hologram nature or properties
- G03H2240/50—Parameters or numerical values associated with holography, e.g. peel strength
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Description
本願は、2009年10月20日出願の米国仮特許出願第61/253,276号、2010年5月5日出願の米国仮特許出願第61/331,500号の権利を主張するものである。米国特許出願第61/253,276号および第61/331,500号は、本書に完全に記載されているように参照により組み込まれる。優先権は、米国特許法119号および他の該当する法律に準じて主張される。
は、画像化センサアレイ16の平面にレンズフリーの画像を作りだす蛍光源分布であり;αは、非負正規化パラメータであり;
は、ベクトル
のlpノームを表している。この作業に用いられる最適化アルゴリズムは 切除型のニュートンの内点法に基づいており、式(1)に基づくスパース蛍光解
に即座に収束する。
に差し替えることができ、実際の画像面における本来の再構成された領域は、以下のように示される。
、すなわちith回反復した後の検出面における複雑な回折領域は、以下のように示すことができる。
全血細胞または微小ビーズなどの小さいまたは僅かに拡散する物体について、両方法が同等の画像の質を有する十分な結果を生じさせる。このような物体について、記録した形態の通常のフレネル数は1未満であり、焦点が合った真の画像は二重像が広がる領域のごく一部を占める。従って、真の画像面における物体画像を除去すると、仮想画像に対して情報の損失が最小限となり、これは二重像の影響なく再生される。しかしながら、大きい対象の物体に対しては、システムのフレネル数が増加し、真の画像を除去することは仮想画像において過剰な情報損失を引き起こすことがあり、反復的に再生させることがより困難となりうる。さらに、強く拡散した物体については、記録された強度のホログラフィックの内容が歪曲しないように、自己および相互の干渉項が支配し始めることがある。従って、強く拡散する物体および/または拡張した物体については、上記の第2の方法は、ホログラフィック項が10未満のフレネル数で機構に支配されることを要する第1の方法よりも好適となる。一方、第1の方法の利点は、試料を機構に挿入する前に取られる別個の背景画像を必ずしも必要としないことである。方法2(ステップb)について背景画像がない状態では、物体平面における領域の平均値を利用することもできるが、最終的な画像の質は、試験的に取得された背景でより良くなることが分かった。
Claims (13)
- 細胞試料を画像化するためのシステムであって:
細胞試料を保持するよう構成された試料ホルダと;
画像センサアレイと、少なくとも1のプロセッサを内部に有する携帯型電子デバイスと;
前記画像センサアレイに近接して前記携帯型電子デバイスに固定されるよう構成された拡張部と;
前記試料ホルダの第1の面において当該試料ホルダからz1の距離で前記拡張部内に配置され、その中に開口を有する空間フィルタであって、前記開口は照明が通過できるよう構成されている空間フィルタと;
前記拡張部内に配置され、前記開口を通って前記細胞試料を照明するよう構成された照明光源であって、前記空間フィルタは前記照明光源と前記試料ホルダの間に置かれている照明光源と;
を具えており、
前記画像センサアレイは、前記携帯型電子デバイス内に配置され、且つ前記試料ホルダの第2の反対側の面において前記試料ホルダからz2の距離に配置され、z2 <z1であり;
前記少なくとも1のプロセッサは、前記画像化センサアレイから前記細胞試料内の関心物体の未加工のホログラム画像を含む画像フレームを受け取るよう構成され、さらに前記関心物体の遺失したホログラム位相を回復し、前記関心物体の画像を再構成するよう構成されていることを特徴とするシステム。 - 請求項1に記載のシステムにおいて、z1 が1cm乃至10cmの範囲内にあり、z2 が0.05mm乃至2cmの範囲内にあることを特徴とするシステム。
- 請求項1に記載のシステムにおいて、前記試料ホルダが前記携帯型電子デバイス内に配置され、細胞試料を保持するよう構成されていることを特徴とするシステム。
- 請求項3に記載のシステムにおいて、前記携帯型電子デバイスが、データを無線送信するように構成されていることを特徴とするシステム。
- 請求項4に記載のシステムにおいて、前記携帯型電子デバイスが、携帯電話または携帯情報端末を含むことを特徴とするシステム。
- 請求項4に記載のシステムにおいて、前記拡張部が、前記携帯型電子デバイスから取り外しできるよう構成されていることを特徴とするシステム。
- 請求項1に記載のシステムにおいて、前記試料ホルダが、マイクロ流体デバイスを具えることを特徴とするシステム。
- 請求項1に記載のシステムがさらに:
前記空間フィルタと前記試料ホルダの間に置かれたプリズムと;
前記プリズムを通して前記細胞試料を照明するよう構成された蛍光照明光源とを具えており、入射蛍光照明が全反射(TIR)によって反射されることを特徴とするシステム。 - 請求項8に記載のシステムがさらに、前記試料ホルダと前記画像化センサアレイの間に置かれた光学フィルタを具えることを特徴とするシステム。
- 請求項9に記載のシステムがさらに、前記プリズムと前記光学フィルタの間に置かれた複数の光ファイバを具える光学フェースプレートを具えていることを特徴とするシステム。
- 細胞試料を画像化する方法であって:
非干渉性の光を発する照明光源を用いて、細胞試料を保持するよう構成された試料ホルダの前面を照明するステップであって、前記非干渉性の光は前記細胞試料を照明する前に開口を通過しており、前記開口は前記試料ホルダからz1の距離に配置され、画像化センサアレイは前記試料ホルダの反対側の面からz2の距離に配置され、z2 <z1であるステップと;
前記試料ホルダの後面に配置された前記画像化センサアレイから1以上の画像フレームを取得するステップであって、前記1以上の画像フレームが前記細胞試料内の関心物体の未加工のホログラム画像を含む、ステップと;
プロセッサを用いて前記関心物体の未加工のホログラム画像を含む前記1以上の画像フレームを処理して、前記画像フレーム内に含まれる前記関心物体の遺失したホログラム位相情報を再生させるステップと;
前記未加工のホログラム画像および前記遺失したホログラム位相情報に少なくとも部分的に基づいて前記関心物体の再構成された画像を前記プロセッサから出力するステップとを含むことを特徴とする方法。 - 請求項11に記載の方法において、前記少なくとも1のプロセッサが1以上の画像フレームとライブラリを比較し、この比較に少なくとも部分的に基づいて、試料内に含まれる1以上のセル、細胞小器官、粒子を識別することを特徴とする方法。
- 請求項11に記載の方法において、前記少なくとも1のプロセッサが、セルの計数を出力することを特徴とする方法。
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PCT/US2010/053225 WO2011049965A1 (en) | 2009-10-20 | 2010-10-19 | Incoherent lensfree cell holography and microscopy on a chip |
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CA2778284A1 (en) | 2011-04-28 |
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