JP5999783B2 - オンチップでの非干渉性のレンズフリーのホログラフィおよび顕微鏡法 - Google Patents
オンチップでの非干渉性のレンズフリーのホログラフィおよび顕微鏡法 Download PDFInfo
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- JP5999783B2 JP5999783B2 JP2014216920A JP2014216920A JP5999783B2 JP 5999783 B2 JP5999783 B2 JP 5999783B2 JP 2014216920 A JP2014216920 A JP 2014216920A JP 2014216920 A JP2014216920 A JP 2014216920A JP 5999783 B2 JP5999783 B2 JP 5999783B2
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- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H2222/00—Light sources or light beam properties
- G03H2222/20—Coherence of the light source
- G03H2222/24—Low coherence light normally not allowing valuable record or reconstruction
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H2222/00—Light sources or light beam properties
- G03H2222/34—Multiple light sources
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H2223/00—Optical components
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- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H2226/00—Electro-optic or electronic components relating to digital holography
- G03H2226/11—Electro-optic recording means, e.g. CCD, pyroelectric sensors
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- G—PHYSICS
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- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H2227/00—Mechanical components or mechanical aspects not otherwise provided for
- G03H2227/02—Handheld portable device, e.g. holographic camera, mobile holographic display
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H2240/00—Hologram nature or properties
- G03H2240/50—Parameters or numerical values associated with holography, e.g. peel strength
- G03H2240/62—Sampling aspect applied to sensor or display
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Description
本願は、2009年10月20日出願の米国仮特許出願第61/253,276号、2010年5月5日出願の米国仮特許出願第61/331,500号の権利を主張するものである。米国特許出願第61/253,276号および第61/331,500号は、本書に完全に記載されているように参照により組み込まれる。優先権は、米国特許法119号および他の該当する法律に準じて主張される。
は、画像化センサアレイ16の平面にレンズフリーの画像を作りだす蛍光源分布であり;αは、非負正規化パラメータであり;
は、ベクトル
のlpノームを表している。この作業に用いられる最適化アルゴリズムは 切除型のニュートンの内点法に基づいており、式(1)に基づくスパース蛍光解
に即座に収束する。
に差し替えることができ、実際の画像面における本来の再構成された領域は、以下のように示される。
、すなわちith回反復した後の検出面における複雑な回折領域は、以下のように示すことができる。
全血細胞または微小ビーズなどの小さいまたは僅かに拡散する物体について、両方法が同等の画像の質を有する十分な結果を生じさせる。このような物体について、記録した形態の通常のフレネル数は1未満であり、焦点が合った真の画像は二重像が広がる領域のごく一部を占める。従って、真の画像面における物体画像を除去すると、仮想画像に対して情報の損失が最小限となり、これは二重像の影響なく再生される。しかしながら、大きい対象の物体に対しては、システムのフレネル数が増加し、真の画像を除去することは仮想画像において過剰な情報損失を引き起こすことがあり、反復的に再生させることがより困難となりうる。さらに、強く拡散した物体については、記録された強度のホログラフィックの内容が歪曲しないように、自己および相互の干渉項が支配し始めることがある。従って、強く拡散する物体および/または拡張した物体については、上記の第2の方法は、ホログラフィック項が10未満のフレネル数で機構に支配されることを要する第1の方法よりも好適となる。一方、第1の方法の利点は、試料を機構に挿入する前に取られる別個の背景画像を必ずしも必要としないことである。方法2(ステップb)について背景画像がない状態では、物体平面における領域の平均値を利用することもできるが、最終的な画像の質は、試験的に取得された背景でより良くなることが分かった。
Claims (6)
- 細胞試料を画像化するためのレンズフリーの顕微鏡システムであって:
1またはそれ以上の細胞または細胞下成分を含む関心物体を中に有する細胞試料を保持するよう構成された試料ホルダと;
前記試料ホルダの片側に、前記試料ホルダからz2の距離に配置され動作領域を有する画像センサアレイと、
前記試料ホルダの反対側に、当該試料ホルダからz1の距離に配置された空間フィルタであって、z2<z1であり、照明が通過できるよう構成されている開口をその中に有する空間フィルタと;
前記開口を通って前記細胞試料を照明するよう構成された照明光源であって、前記空間フィルタは前記照明光源と前記試料ホルダの間に置かれており、前記照明光源と前記細胞試料の間にレンズがなく、前記試料ホルダと前記画像センサアレイの間にレンズがない、照明光源と;
を具えており、
前記少なくとも1のプロセッサは、前記画像化センサアレイから前記細胞試料内の関心物体の未加工のホログラム画像を含む画像フレームを受け取るよう構成され、さらに前記関心物体の遺失したホログラム位相を回復し、細胞下分解能を有し前記画像センサアレイの動作領域と等しい視野を有する顕微鏡品質の前記関心物体の画像を再構成するよう構成されていることを特徴とするシステム。 - 請求項1に記載のレンズフリーの顕微鏡システムにおいて、z1が1cm乃至10cmの範囲内にあり、z2が0.05mm乃至2cmの範囲内にあることを特徴とするシステム。
- 請求項1に記載のレンズフリーの顕微鏡システムにおいて、前記試料ホルダが、マイクロ流体デバイスを具えることを特徴とするシステム。
- 請求項1に記載のレンズフリーの顕微鏡システムがさらに:
前記空間フィルタと前記試料ホルダの間に置かれたプリズムと;
前記プリズムを通して前記細胞試料を照明するよう構成された蛍光照明光源とを具えており、入射蛍光照明が全反射(TIR)によって反射されることを特徴とするシステム。 - 請求項4に記載のレンズフリーの顕微鏡システムがさらに、前記試料ホルダと前記画像化センサアレイの間に置かれた光学フィルタを具えることを特徴とするシステム。
- 請求項5に記載のレンズフリーの顕微鏡システムがさらに、前記プリズムと前記光学フィルタの間に置かれた複数の光ファイバを具える光学フェースプレートを具えていることを特徴とするシステム。
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