JP5420837B2 - 胚幹細胞の培地及び培養 - Google Patents

胚幹細胞の培地及び培養 Download PDF

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JP5420837B2
JP5420837B2 JP2007531300A JP2007531300A JP5420837B2 JP 5420837 B2 JP5420837 B2 JP 5420837B2 JP 2007531300 A JP2007531300 A JP 2007531300A JP 2007531300 A JP2007531300 A JP 2007531300A JP 5420837 B2 JP5420837 B2 JP 5420837B2
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stem cells
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JP2008512122A (ja
JP2008512122A5 (enExample
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ジェイムズ エイ トムソン
テナイル ルードウィッグ
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ウィスコンシン アラムニ リサーチ ファンデーション
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    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/845—Gamma amino butyric acid [GABA]

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JP2007531300A 2004-09-08 2005-09-08 胚幹細胞の培地及び培養 Expired - Lifetime JP5420837B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US60804004P 2004-09-08 2004-09-08
US60/608,040 2004-09-08
PCT/US2005/031838 WO2006029197A1 (en) 2004-09-08 2005-09-08 Medium and culture of embryonic stem cells

Related Child Applications (2)

Application Number Title Priority Date Filing Date
JP2011165510A Division JP2011206064A (ja) 2004-09-08 2011-07-28 胚幹細胞の培地及び培養
JP2013202449A Division JP2014014373A (ja) 2004-09-08 2013-09-27 胚幹細胞の培地及び培養

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JP2008512122A JP2008512122A (ja) 2008-04-24
JP2008512122A5 JP2008512122A5 (enExample) 2013-11-21
JP5420837B2 true JP5420837B2 (ja) 2014-02-19

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JP2007531300A Expired - Lifetime JP5420837B2 (ja) 2004-09-08 2005-09-08 胚幹細胞の培地及び培養
JP2011165510A Pending JP2011206064A (ja) 2004-09-08 2011-07-28 胚幹細胞の培地及び培養
JP2013202449A Pending JP2014014373A (ja) 2004-09-08 2013-09-27 胚幹細胞の培地及び培養

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JP2011165510A Pending JP2011206064A (ja) 2004-09-08 2011-07-28 胚幹細胞の培地及び培養
JP2013202449A Pending JP2014014373A (ja) 2004-09-08 2013-09-27 胚幹細胞の培地及び培養

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US (5) US7449334B2 (enExample)
EP (1) EP1791948B1 (enExample)
JP (3) JP5420837B2 (enExample)
KR (1) KR101264940B1 (enExample)
CN (2) CN101044235B (enExample)
AT (1) ATE549395T1 (enExample)
AU (1) AU2005282510B2 (enExample)
BR (1) BRPI0514778B1 (enExample)
CA (1) CA2579643C (enExample)
ES (1) ES2383813T3 (enExample)
GB (1) GB2432846B (enExample)
IL (1) IL181437A (enExample)
IS (1) IS8618A (enExample)
MX (1) MX2007002390A (enExample)
NO (1) NO344695B1 (enExample)
NZ (1) NZ553241A (enExample)
WO (1) WO2006029197A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200034331A (ko) * 2018-09-21 2020-03-31 메디포스트(주) 피부 세포 활성화용 조성물 및 이의 용도

Families Citing this family (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3196296T3 (en) * 2004-09-08 2019-02-04 Wisconsin Alumini Res Foundation Cultivation of human embryonic stem cells
ES2383813T3 (es) * 2004-09-08 2012-06-26 Wisconsin Alumni Research Foundation Método de cultivo y cultivo de células madre embrionarias
US8017395B2 (en) 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
AU2006255183B2 (en) 2005-06-08 2012-02-02 Centocor, Inc. A cellular therapy for ocular degeneration
US20070025973A1 (en) * 2005-07-29 2007-02-01 Fitzsimmons Frederick J Reprogramming of adult or neonic stem cells and methods of use
CA2624728A1 (en) * 2005-10-07 2007-04-19 Cellartis Ab A method for obtaining a xeno-free hbs cell line
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
GB0613756D0 (en) * 2006-07-12 2006-08-23 Univ Sheffield Cell culture medium
WO2008089351A1 (en) * 2007-01-17 2008-07-24 Wisconsin Alumni Research Foundation Improved culture of stem cells
EP2139988A1 (en) * 2007-03-15 2010-01-06 California Stem Cell, Inc. Cardiomyocytes and methods of producing and purifying cardiomyocytes
FI20075417A0 (fi) * 2007-06-05 2007-06-05 Marjo-Riitta Suuronen Koostumuksia ja menetelmiä alkion kantasolujen kasvatukseen
US8815585B2 (en) * 2007-06-29 2014-08-26 Cellular Dynamics International, Inc. Automated method and apparatus for embryonic stem cell culture
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
CA2695225C (en) 2007-07-31 2021-06-01 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic endocrine
US9062290B2 (en) 2007-11-27 2015-06-23 Lifescan, Inc. Differentiation of human embryonic stem cells
US20110008893A1 (en) * 2007-12-28 2011-01-13 Fujirebio Inc. Medium for mammalian somatic cells and additive therefor
BRPI0908033A2 (pt) 2008-02-21 2015-08-04 Centocor Ortho Biotech Inc Método placas de superfície modificada e composições para adesão, cultura e desprendimento de célula
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
WO2009135206A1 (en) * 2008-05-02 2009-11-05 Stem Cell Products, Inc. Method for production of mast cells from stem cells
BRPI0914116A2 (pt) 2008-06-30 2019-09-17 Centocor Ortho Biotech Inc diferenciação de células-tronco pluripotentes
MX2011000123A (es) * 2008-06-30 2011-02-25 Centocor Ortho Biotech Inc Diferenciacion de las celulas madre pluripotentes.
WO2010017562A2 (en) 2008-08-08 2010-02-11 Mayo Foundation For Medical Education And Research Induced pluripotent stem cells
EP2346988B1 (en) 2008-10-31 2017-05-31 Janssen Biotech, Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
BRPI0919883A2 (pt) 2008-10-31 2015-08-11 Centocor Ortho Biotech Inc Diferenciação de células-tronco embrionárias humanas para a linhagem endócrina pancreática
ES2642070T3 (es) 2008-11-20 2017-11-15 Janssen Biotech, Inc. Cultivo de células madre pluripotentes en microportadores
CN102257132B (zh) 2008-11-20 2014-09-03 森托科尔奥索生物科技公司 用于在平面基底上进行细胞附着和培养的方法和组合物
US8557580B2 (en) * 2009-02-20 2013-10-15 Cellular Dynamics International, Inc. Methods and compositions for the differentiation of stem cells
KR101720961B1 (ko) * 2009-02-27 2017-03-29 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 다능성 세포의 분화
AU2010276402B2 (en) 2009-07-20 2014-07-03 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
BR112012001480A2 (pt) 2009-07-20 2015-09-01 Janssen Biotech Inc Diferenciação de células-tronco embriônicas humanas
AU2010276440B2 (en) 2009-07-20 2014-07-03 Janssen Biotech Inc. Differentiation of human embryonic stem cells
JP5957382B2 (ja) * 2009-10-29 2016-07-27 ヤンセン バイオテツク，インコーポレーテツド 多能性幹細胞
WO2011063005A2 (en) 2009-11-17 2011-05-26 Advanced Cell Technology, Inc. Methods of producing human rpe cells and pharmaceutical preparations of human rpe cells
FI20096288A0 (fi) 2009-12-04 2009-12-04 Kristiina Rajala Formulations and methods for culturing stem cells
EP2516626B1 (en) 2009-12-23 2017-05-10 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
WO2011079017A2 (en) 2009-12-23 2011-06-30 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells
WO2011109279A2 (en) 2010-03-01 2011-09-09 Centocor Ortho Biotech Inc. Methods for purifying cells derived from pluripotent stem cells
EP3498825A1 (en) 2010-05-12 2019-06-19 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
AU2010355614B2 (en) 2010-06-13 2015-07-02 Institute Of Biophysics, Chinese Academy Of Sciences Methods and compositions for preparing cardiomyocytes from stem cells and uses thereof
WO2012012803A2 (en) 2010-07-23 2012-01-26 Advanced Cell Technology, Inc. Methods for detection of rare subpopulations of cells and highly purified compositions of cells
US9279107B2 (en) 2010-08-05 2016-03-08 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture
WO2012019122A2 (en) 2010-08-05 2012-02-09 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture
ES2659393T3 (es) 2010-08-31 2018-03-15 Janssen Biotech, Inc. Diferenciación de células madre embrionarias humanas
US9506036B2 (en) 2010-08-31 2016-11-29 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
RU2673946C1 (ru) 2010-08-31 2018-12-03 Янссен Байотек, Инк. Дифференцирование плюрипотентных стволовых клеток
CN102559587A (zh) * 2010-12-16 2012-07-11 中国科学院上海药物研究所 诱导性多能干细胞的制备方法以及用于制备诱导性多能干细胞的培养基
EP2651973B1 (en) * 2010-12-17 2016-08-31 Biolamina AB Recombinant laminin-521
US9133266B2 (en) * 2011-05-06 2015-09-15 Wisconsin Alumni Research Foundation Vitronectin-derived cell culture substrate and uses thereof
US20130029416A1 (en) 2011-07-22 2013-01-31 Tayaramma Thatava Differentiating induced pluripotent stem cells into glucose-responsive, insulin-secreting progeny
WO2013022584A1 (en) 2011-08-05 2013-02-14 Corning Incorporated Modulation of the mammalian receptor mtor to inhibit stem cell differentiation into neurons
CN102399742B (zh) * 2011-09-30 2014-07-30 东莞中山大学研究院 用于哺乳动物唾液腺上皮细胞和唾液腺干细胞培养的无血清培养液
WO2013082268A1 (en) 2011-11-30 2013-06-06 The Wistar Institute Of Anatomy And Biology Methods and compositions for regulation of cell aging, carcinogenesis, and reprogramming
BR112014013664A2 (pt) 2011-12-05 2020-11-03 Factor Bioscience Inc. métodos e produtos para transfectar células
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
US8772460B2 (en) 2011-12-16 2014-07-08 Wisconsin Alumni Research Foundation Thermostable FGF-2 mutant having enhanced stability
EP2794857A4 (en) 2011-12-22 2015-07-08 Janssen Biotech Inc DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS IN SINGLE INSULIN POSITIVE CELLS
HK1206058A1 (en) * 2012-03-07 2015-12-31 Janssen Biotech, Inc. Defined media for expansion and maintenance of pluripotent stem cells
AU2013251785B2 (en) 2012-04-24 2018-05-10 Dan S. Kaufman Method for developing natural killer cells from stem cells
ES2897649T3 (es) 2012-06-08 2022-03-02 Janssen Biotech Inc Diferenciación de células madre embrionarias humanas en células endocrinas pancreáticas
GB201211873D0 (en) * 2012-07-04 2012-08-15 Univ Edinburgh Cell culture
WO2014015777A1 (zh) 2012-07-23 2014-01-30 中国科学院生物物理研究所 体外诱导多能干细胞分化为心室肌细胞的方法
BR122019025681B1 (pt) 2012-11-01 2023-04-18 Factor Bioscience Inc Método para inserir uma sequência de ácido nucleico em uma localização segura de um genoma de uma célula
RU2687379C2 (ru) 2012-12-31 2019-05-13 Янссен Байотек, Инк. Суспендирование и кластеризация плюрипотентных клеток человека с целью их дифференцировки в панкреатические эндокринные клетки
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
AU2013368221B2 (en) 2012-12-31 2018-11-01 Janssen Biotech, Inc. Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
EP4219683A1 (en) 2012-12-31 2023-08-02 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells using hb9 regulators
CA3141731A1 (en) 2013-03-13 2014-10-09 Wisconsin Alumni Research Foundation Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions
NL2011170C2 (en) * 2013-07-15 2015-01-21 Univ Erasmus Medical Ct Method and culture medium for in vitro culturing of stem cells.
WO2015042356A1 (en) 2013-09-19 2015-03-26 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
US9770489B2 (en) 2014-01-31 2017-09-26 Factor Bioscience Inc. Methods and products for nucleic acid production and delivery
CN106459918A (zh) 2014-04-24 2017-02-22 得克萨斯州大学系统董事会 施加诱导多能干细胞产生过继细胞疗法产品
WO2015171663A2 (en) * 2014-05-05 2015-11-12 Indiana University Research And Technology Corporation Method for producing retinal ganglion cells from pluripotent cells
CA2949056C (en) 2014-05-16 2025-10-07 Janssen Biotech, Inc. USE OF SMALL MOLECULES TO IMPROVE MAFA GENE EXPRESSION IN PANCREATIC ENDOCRINE CELLS
US11241505B2 (en) 2015-02-13 2022-02-08 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
CN104830772A (zh) * 2015-05-28 2015-08-12 深圳富利鑫健康产业发展有限公司 一种造血干细胞培养基及其应用和干细胞培养方法
ES2862676T3 (es) * 2015-10-20 2021-10-07 Fujifilm Cellular Dynamics Inc Producción de células precursoras hematopoyéticas multilinaje mediante programación genética
MA45479A (fr) 2016-04-14 2019-02-20 Janssen Biotech Inc Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen
US10576167B2 (en) 2016-08-17 2020-03-03 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
EP4361256A3 (en) * 2017-01-23 2024-07-31 Stemcell Technologies Canada Inc. Media and methods for enhancing the survival and proliferation of stem cells
AU2018254442B2 (en) 2017-04-18 2024-03-28 FUJIFILM Cellular Dynamics, Inc. Antigen-specific immune effector cells
CA3064864A1 (en) * 2017-05-25 2018-11-29 New York Stem Cell Foundation, Inc. Method and composition for generating basal forebrain cholinergic neurons (bfcns)
CA3068685A1 (en) * 2017-06-27 2019-01-03 Ajinomoto Co., Inc. Riboflavin derivative-containing medium
KR102121042B1 (ko) * 2017-12-06 2020-06-09 이정복 조류의 에그로부터 분리한 세포인 배반엽 전분화능세포, 신경줄기세포, 배아섬유아세포 컨디션드 배지를 유효성분으로 하는 세포 증식, 피부재생 및 주름 개선용 배지 조성물
WO2019177420A1 (ko) * 2018-03-16 2019-09-19 사회복지법인 삼성생명공익재단 Fgf-17을 유효 성분으로 포함하는 줄기세포 증식 촉진용 배지 조성물 및 이를 이용한 줄기세포 증식 촉진 방법
KR20220039719A (ko) 2019-07-03 2022-03-29 팩터 바이오사이언스 인크. 양이온성 지질 및 이의 용도
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods
CN111690595B (zh) * 2020-07-21 2021-07-16 生物岛实验室 一种无血清无饲养层的胚胎干细胞或多能干细胞培养基及其应用
CN112029708A (zh) * 2020-09-09 2020-12-04 江苏育瑞康生物科技有限公司 一种干细胞培养系统

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3880795B2 (ja) * 1997-10-23 2007-02-14 ジェロン・コーポレーション フィーダー細胞を含まない培養物中で、霊長類由来始原幹細胞を増殖させるための方法
US7410798B2 (en) * 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US7455983B2 (en) * 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
US7514260B2 (en) * 2004-05-21 2009-04-07 Wicell Research Institute, Inc. Feeder independent extended culture of embryonic stem cells
US7005252B1 (en) * 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
SI1572984T1 (sl) * 2002-12-16 2016-07-29 Technion Research & Development Foundation Ltd. Kulturni sistem brez hranilnih celic, brez kseno-komponent, za humane embrionalne matične celice
KR100632851B1 (ko) 2004-01-20 2006-10-13 한국원자력연구소 전분화능 줄기세포의 분화를 억제하는 방법
DK3196296T3 (en) * 2004-09-08 2019-02-04 Wisconsin Alumini Res Foundation Cultivation of human embryonic stem cells
ES2383813T3 (es) * 2004-09-08 2012-06-26 Wisconsin Alumni Research Foundation Método de cultivo y cultivo de células madre embrionarias

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200034331A (ko) * 2018-09-21 2020-03-31 메디포스트(주) 피부 세포 활성화용 조성물 및 이의 용도
KR102216302B1 (ko) 2018-09-21 2021-02-17 메디포스트(주) 피부 세포 활성화용 조성물 및 이의 용도

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