JP5246878B2 - シルクタンパク質を利用した人工鼓膜 - Google Patents
シルクタンパク質を利用した人工鼓膜 Download PDFInfo
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- JP5246878B2 JP5246878B2 JP2009154428A JP2009154428A JP5246878B2 JP 5246878 B2 JP5246878 B2 JP 5246878B2 JP 2009154428 A JP2009154428 A JP 2009154428A JP 2009154428 A JP2009154428 A JP 2009154428A JP 5246878 B2 JP5246878 B2 JP 5246878B2
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- silk
- eardrum
- artificial
- membrane
- silk protein
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- 210000003454 tympanic membrane Anatomy 0.000 title claims abstract description 63
- 108090000623 proteins and genes Proteins 0.000 title abstract description 36
- 102000004169 proteins and genes Human genes 0.000 title abstract description 36
- 239000012528 membrane Substances 0.000 claims abstract description 28
- 108010022355 Fibroins Proteins 0.000 claims abstract description 24
- 108010013296 Sericins Proteins 0.000 claims abstract description 15
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract description 15
- 239000000835 fiber Substances 0.000 claims abstract description 12
- 241000255789 Bombyx mori Species 0.000 claims abstract description 7
- 102000008186 Collagen Human genes 0.000 claims abstract description 6
- 108010035532 Collagen Proteins 0.000 claims abstract description 6
- 239000000783 alginic acid Substances 0.000 claims abstract description 6
- 229920000615 alginic acid Polymers 0.000 claims abstract description 6
- 235000010443 alginic acid Nutrition 0.000 claims abstract description 6
- 229960001126 alginic acid Drugs 0.000 claims abstract description 6
- 150000004781 alginic acids Chemical class 0.000 claims abstract description 6
- 229920001436 collagen Polymers 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 17
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 15
- 239000002202 Polyethylene glycol Substances 0.000 claims description 14
- 239000000654 additive Substances 0.000 claims description 10
- 230000000996 additive effect Effects 0.000 claims description 10
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 8
- 150000003839 salts Chemical class 0.000 claims description 7
- 239000007864 aqueous solution Substances 0.000 claims description 6
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 5
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 5
- 229920001661 Chitosan Polymers 0.000 claims description 5
- 108010010803 Gelatin Proteins 0.000 claims description 5
- 229940045110 chitosan Drugs 0.000 claims description 5
- 229960005188 collagen Drugs 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 5
- 229920000159 gelatin Polymers 0.000 claims description 5
- 239000008273 gelatin Substances 0.000 claims description 5
- 229940014259 gelatin Drugs 0.000 claims description 5
- 235000019322 gelatine Nutrition 0.000 claims description 5
- 235000011852 gelatine desserts Nutrition 0.000 claims description 5
- 229920002674 hyaluronan Polymers 0.000 claims description 5
- 229960003160 hyaluronic acid Drugs 0.000 claims description 5
- AMXOYNBUYSYVKV-UHFFFAOYSA-M lithium bromide Chemical compound [Li+].[Br-] AMXOYNBUYSYVKV-UHFFFAOYSA-M 0.000 claims description 4
- 239000011592 zinc chloride Substances 0.000 claims description 4
- 235000005074 zinc chloride Nutrition 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 3
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- 239000003242 anti bacterial agent Substances 0.000 claims description 3
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- 238000000034 method Methods 0.000 description 15
- 206010045210 Tympanic Membrane Perforation Diseases 0.000 description 14
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
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- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
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- 241000228212 Aspergillus Species 0.000 description 1
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- 229920000298 Cellophane Polymers 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
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- 230000003213 activating effect Effects 0.000 description 1
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- 150000001298 alcohols Chemical class 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
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Images
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F11/00—Methods or devices for treatment of the ears or hearing sense; Non-electric hearing aids; Methods or devices for enabling ear patients to achieve auditory perception through physiological senses other than hearing sense; Protective devices for the ears, carried on the body or in the hand
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/18—Internal ear or nose parts, e.g. ear-drums
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/18—Internal ear or nose parts, e.g. ear-drums
- A61F2002/183—Ear parts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/14—Materials or treatment for tissue regeneration for ear reconstruction or ear implants, e.g. implantable hearing aids
Description
Claims (5)
- シルクフィブロインを利用した人工鼓膜であって、
繭又は絹繊維からセリシンを除去した後に得られたシルクフィブロイン溶液を脱塩及び乾燥することにより、空隙がない透明なシルク膜形態の構造を有することを特徴とする人工鼓膜。 - 前記シルク膜が、0.8%乃至20%濃度のシルクフィブロイン溶液を用いて室温乃至90℃の温度条件で製造されることを特徴とする請求項1に記載の人工鼓膜。
- 前記シルクフィブロイン溶液が、ブロム化リチウム(LiBr)、塩化リチウム(LiCl2)、塩化亜鉛(ZnCl2)又は塩化カルシウム(CaCl2)のうち少なくともいずれか1つの化合物又はこれを含むエタノール水溶液からなるカオトロピック塩を使用して溶解されることを特徴とする請求項1に記載の人工鼓膜。
- 前記シルク膜が、ゼラチン、コラーゲン、キトサン、アルギン酸、ヒアルロン酸、又はPEG(Polyethylene glycol)のうち少なくともいずれか1つの添加剤をさらに含むことを特徴とする請求項1に記載の人工鼓膜。
- 前記シルク膜が、適用部位の追加感染を防止するために、可塑剤、柔軟剤、抗生剤、抗微生物剤、細胞、酵素、抗体又は顔料のうち少なくともいずれか1つの添加剤又はこれらの混合物よりなる群から選択されるいずれか1つの添加剤をさらに含むことを特徴とする請求項1に記載の人工鼓膜。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020090040200A KR101060910B1 (ko) | 2009-05-08 | 2009-05-08 | 실크단백질을 이용한 인공고막 및 그 제조방법 |
KR10-2009-0040200 | 2009-05-08 |
Publications (2)
Publication Number | Publication Date |
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JP2010259767A JP2010259767A (ja) | 2010-11-18 |
JP5246878B2 true JP5246878B2 (ja) | 2013-07-24 |
Family
ID=42646319
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP2009154428A Active JP5246878B2 (ja) | 2009-05-08 | 2009-06-29 | シルクタンパク質を利用した人工鼓膜 |
Country Status (5)
Country | Link |
---|---|
US (1) | US8500808B2 (ja) |
EP (1) | EP2251048B1 (ja) |
JP (1) | JP5246878B2 (ja) |
KR (1) | KR101060910B1 (ja) |
CN (1) | CN101879098B (ja) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
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KR101322337B1 (ko) * | 2010-12-23 | 2013-10-25 | 대한민국 | 유연성과 봉합강도가 우수한 인공뇌경막 및 생체막용 실크 인공막 |
CN103764071B (zh) * | 2011-07-11 | 2017-02-15 | 澳大利亚耳科学研究所 | 鼓室修补器械 |
TWI448296B (zh) * | 2012-05-04 | 2014-08-11 | Wen Fu Lai | 修復耳膜之醫藥組成物及其用途 |
WO2013185173A1 (en) | 2012-06-12 | 2013-12-19 | The University Of Western Australia | Method for producing a collagen membrane and uses thereof |
EP2712955A1 (en) | 2012-09-27 | 2014-04-02 | Ludwig Boltzmann Gesellschaft GmbH | Product made of silk |
CN103114491A (zh) * | 2013-02-27 | 2013-05-22 | 盐城纺织职业技术学院 | 一种含胶类纤维成膜方法及其装置 |
CN103341214B (zh) * | 2013-07-08 | 2014-12-31 | 苏州大学 | 一种丝素蛋白膜及其制备方法 |
CN103341209B (zh) * | 2013-07-08 | 2015-04-08 | 苏州大学 | 一种丝素蛋白纳米纤维膜及其制备方法 |
KR101573838B1 (ko) * | 2014-06-13 | 2015-12-07 | 대한민국 | 누에고치를 이용한 인공 생체막 및 이의 제조방법 |
KR101570717B1 (ko) * | 2014-08-04 | 2015-11-24 | 대한민국 | 누에고치를 이용한 치과용 차폐막 및 이의 제조방법 |
KR101629204B1 (ko) | 2014-08-19 | 2016-06-10 | 전북대학교산학협력단 | 초박막 실크피브로인/콜라겐 복합이식체 및 이의 제조방법 |
KR101640499B1 (ko) * | 2015-08-19 | 2016-07-18 | 한림대학교 산학협력단 | 형질 전환 누에고치로부터 추출한 형광 실크 단백질 용액의 제조 방법 및 이를 이용한 지지체 제조 방법 |
KR101602797B1 (ko) * | 2015-10-21 | 2016-03-11 | 대한민국 | 평면견을 이용한 인공 생체막 및 이의 제조방법 |
US11365292B2 (en) * | 2016-03-31 | 2022-06-21 | Ear Science Institute Australia | Silk fibroin glycerol membranes |
CN109069699B (zh) * | 2016-04-14 | 2021-11-16 | 澳大利亚耳科学研究所 | 改善的丝纤蛋白生物相容性聚氨酯膜 |
CN106290325B (zh) * | 2016-07-19 | 2019-05-03 | 南通纺织丝绸产业技术研究院 | 蚕丝蛋白薄膜在制备湿度传感器中的应用 |
CN106139246A (zh) * | 2016-08-19 | 2016-11-23 | 苏州大学 | 一种再生丝蛋白纤维支架及其制备方法 |
CN107510863A (zh) * | 2017-08-25 | 2017-12-26 | 张阳 | 一种复合促神经修复管及其制备方法 |
CN107552095A (zh) * | 2017-09-17 | 2018-01-09 | 钱景 | 负载二氧化钛‑纳米银的蚕丝三维多孔材料及制备方法 |
CN110421915B (zh) * | 2019-07-05 | 2021-07-30 | 厦门派恩杰科技有限公司 | 一种具有加热控温功能的蛋白质薄膜的制备方法 |
KR102228268B1 (ko) | 2020-08-13 | 2021-03-16 | 한국실크연구원 | 실크볼을 이용한 카본 제조장치 |
CN113209388A (zh) * | 2021-03-30 | 2021-08-06 | 复旦大学附属眼耳鼻喉科医院 | 一种传递声音和诱导鼓膜再生的纤维膜及其制备方法和应用 |
CN113278170B (zh) * | 2021-06-15 | 2021-11-16 | 苏州大学 | 一种化学交联透明质酸水凝胶及其制备方法与应用 |
CN114835933A (zh) * | 2022-05-20 | 2022-08-02 | 西南大学 | Pluronic高分子高韧性丝素复合透明薄膜制备方法及制品 |
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- 2009-06-29 JP JP2009154428A patent/JP5246878B2/ja active Active
- 2009-06-29 US US12/493,277 patent/US8500808B2/en active Active
- 2009-06-29 EP EP09164040.9A patent/EP2251048B1/en active Active
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KR101060910B1 (ko) | 2011-08-30 |
KR20100121169A (ko) | 2010-11-17 |
EP2251048A3 (en) | 2011-09-07 |
US8500808B2 (en) | 2013-08-06 |
EP2251048B1 (en) | 2014-04-09 |
EP2251048A2 (en) | 2010-11-17 |
JP2010259767A (ja) | 2010-11-18 |
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