JP4777981B2 - 共鳴エネルギー移動中における蛍光の信号検出を改善する方法 - Google Patents

共鳴エネルギー移動中における蛍光の信号検出を改善する方法 Download PDF

Info

Publication number
JP4777981B2
JP4777981B2 JP2007517367A JP2007517367A JP4777981B2 JP 4777981 B2 JP4777981 B2 JP 4777981B2 JP 2007517367 A JP2007517367 A JP 2007517367A JP 2007517367 A JP2007517367 A JP 2007517367A JP 4777981 B2 JP4777981 B2 JP 4777981B2
Authority
JP
Japan
Prior art keywords
wavelength
donor
plane
polarization
fluorescent compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2007517367A
Other languages
English (en)
Japanese (ja)
Other versions
JP2008504517A (ja
JP2008504517A5 (enExample
Inventor
トリンク,エリック
マティ,ジェラール
Original Assignee
シ ビオ アンテルナショナル
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by シ ビオ アンテルナショナル filed Critical シ ビオ アンテルナショナル
Publication of JP2008504517A publication Critical patent/JP2008504517A/ja
Publication of JP2008504517A5 publication Critical patent/JP2008504517A5/ja
Application granted granted Critical
Publication of JP4777981B2 publication Critical patent/JP4777981B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6445Measuring fluorescence polarisation

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
JP2007517367A 2004-06-28 2005-06-27 共鳴エネルギー移動中における蛍光の信号検出を改善する方法 Expired - Lifetime JP4777981B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0407087A FR2872287B1 (fr) 2004-06-28 2004-06-28 Procede d'amelioration de la detection des signaux de fluorescence lors d'un transfert d'energie non radiatif
FR0407087 2004-06-28
PCT/FR2005/001618 WO2006010839A2 (fr) 2004-06-28 2005-06-27 Procede d’amelioration de la detection des signaux de fluorescence lors d’un transfert d’energie non radiatif

Publications (3)

Publication Number Publication Date
JP2008504517A JP2008504517A (ja) 2008-02-14
JP2008504517A5 JP2008504517A5 (enExample) 2008-05-22
JP4777981B2 true JP4777981B2 (ja) 2011-09-21

Family

ID=34946931

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2007517367A Expired - Lifetime JP4777981B2 (ja) 2004-06-28 2005-06-27 共鳴エネルギー移動中における蛍光の信号検出を改善する方法

Country Status (5)

Country Link
US (1) US7872243B2 (enExample)
EP (1) EP1766374A2 (enExample)
JP (1) JP4777981B2 (enExample)
FR (1) FR2872287B1 (enExample)
WO (1) WO2006010839A2 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2872287B1 (fr) 2004-06-28 2007-03-16 Cis Bio Internat Sa Procede d'amelioration de la detection des signaux de fluorescence lors d'un transfert d'energie non radiatif
US8031338B2 (en) * 2004-12-02 2011-10-04 Vanderbilt University Measuring Forster resonance energy transfer with polarized and depolarized light
WO2006107864A1 (en) 2005-04-04 2006-10-12 Blueshift Biotechnologies, Inc. Screening using polarization anisotropy in fret emissions
GB0603636D0 (en) * 2006-02-23 2006-04-05 Advanced Sensors Ltd Apparatus for measuring fluorescent material in a liquid
US8269191B2 (en) * 2010-11-05 2012-09-18 The United States Of America As Represented By The Secretary Of The Army System and method for measuring depolarization
KR101320097B1 (ko) * 2011-06-23 2013-10-18 한국생명공학연구원 고유 형광 공명 에너지 전이용 탐침을 이용한 단백질 검출 또는 이미지화 현미경 장치 및 이를 이용한 단백질 검출 또는 이미징방법
EP2797635B1 (en) 2011-12-30 2020-05-20 University of Washington Through Its Center for Commercialization Chromophoric polymer dots with narrow-band emission
US20150289372A1 (en) * 2014-04-03 2015-10-08 Yikang Deng Fluorescent conductive fill material for plated through hole structures and methods of defect inspection utilizing the same
CN105466902A (zh) * 2016-01-18 2016-04-06 华南师范大学 一种荧光共振能量转移敏化淬灭转化因子的测量方法
CN105842210B (zh) * 2016-03-23 2018-08-24 南昌大学 基于生物量子点和Au NPs荧光共振能量转移的凝血酶检测方法
CN106442455B (zh) * 2016-11-23 2019-01-08 华南师范大学 一种基于双通道荧光强度同时探测的荧光共振能量转移效率的快速测量方法
CN112763463A (zh) * 2020-11-05 2021-05-07 北京工业大学 一种提高回音壁模式微腔传感灵敏度的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002098638A (ja) * 2000-09-26 2002-04-05 Shimadzu Corp 蛍光偏光度測定装置
HU226937B1 (en) * 2000-11-17 2010-03-29 Mta Szegedi Biolog Koezpont Method and apparatus for determining polarization amount of material by a laser scanning microscope
US20030228703A1 (en) * 2002-04-05 2003-12-11 The Regents Of The University Of Michigan Fluorescence resonance energy transfer quantitation and stoichiometry in living cells
JP4214206B2 (ja) * 2002-12-10 2009-01-28 独立行政法人理化学研究所 Fretを利用した蛍光指示薬
FR2872287B1 (fr) 2004-06-28 2007-03-16 Cis Bio Internat Sa Procede d'amelioration de la detection des signaux de fluorescence lors d'un transfert d'energie non radiatif

Also Published As

Publication number Publication date
FR2872287B1 (fr) 2007-03-16
EP1766374A2 (fr) 2007-03-28
US20090294691A1 (en) 2009-12-03
US7872243B2 (en) 2011-01-18
JP2008504517A (ja) 2008-02-14
WO2006010839A2 (fr) 2006-02-02
WO2006010839B1 (fr) 2006-08-17
WO2006010839A3 (fr) 2006-06-22
FR2872287A1 (fr) 2005-12-30

Similar Documents

Publication Publication Date Title
Dunsing et al. Optimal fluorescent protein tags for quantifying protein oligomerization in living cells
Sahoo Förster resonance energy transfer–A spectroscopic nanoruler: Principle and applications
Schwille Fluorescence correlation spectroscopy
Daly et al. Fluorescent ligands, antibodies, and proteins for the study of receptors
JP4511363B2 (ja) 相互作用する分子を検出するためのfretプローブおよび方法
JP4777981B2 (ja) 共鳴エネルギー移動中における蛍光の信号検出を改善する方法
Nguyen et al. Fluorescence polarization and fluctuation analysis monitors subunit proximity, stoichiometry, and protein complex hydrodynamics
Schuler Application of single molecule Förster resonance energy transfer to protein folding
Masi et al. Optical methods in the study of protein-protein interactions
Schuler et al. Application of confocal single-molecule FRET to intrinsically disordered proteins
Ojha et al. Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association
KR20200024736A (ko) 타겟 물질 검출을 위한 방법 및 키트
US20150185150A1 (en) Method for normalizing the luminescence emitted by a measuring medium
Ujlaky-Nagy et al. Flow cytometric FRET analysis of protein interactions
Laskaratou et al. Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor
Ma et al. Fluorescence cross-correlation spectroscopy (FCCS) in living cells
JP2008504517A5 (enExample)
Wang et al. Studying structural dynamics of potassium channels by single-molecule FRET
Strianese et al. Modern fluorescence-based concepts and methods to study biomolecular interactions
US20140170769A1 (en) Microscope apparatus for detecting or imaging protein using probe for intrinsic fluorescence resonance energy transfer and method for detecting or imaging protein using the same
Vereb et al. Flow cytometric FRET analysis of protein interaction
FR3069644A1 (fr) Methode pour mesurer la modulation de l'activation d'un recepteur couple a une proteine g
Subadini et al. Fluorescence Resonance Energy Transfer: A Technique to Unleash Biomolecular Structure and Dynamics
EP1802975B1 (en) Method for identifying compounds that affect a transport of a protein through a membrane trafficking pathway
JP7007132B2 (ja) アミロイド凝集体の検出方法、アミロイド凝集体検出装置、及びアミロイド凝集体検出プログラム

Legal Events

Date Code Title Description
A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20080331

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20080331

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20100816

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20101130

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20110218

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20110225

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110511

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20110614

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20110630

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

Ref document number: 4777981

Country of ref document: JP

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20140708

Year of fee payment: 3

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

EXPY Cancellation because of completion of term