WO2006010839A3 - Procede d’amelioration de la detection des signaux de fluorescence lors d’un transfert d’energie non radiatif - Google Patents

Procede d’amelioration de la detection des signaux de fluorescence lors d’un transfert d’energie non radiatif Download PDF

Info

Publication number
WO2006010839A3
WO2006010839A3 PCT/FR2005/001618 FR2005001618W WO2006010839A3 WO 2006010839 A3 WO2006010839 A3 WO 2006010839A3 FR 2005001618 W FR2005001618 W FR 2005001618W WO 2006010839 A3 WO2006010839 A3 WO 2006010839A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescence
energy transfer
signals during
resonance energy
improving detection
Prior art date
Application number
PCT/FR2005/001618
Other languages
English (en)
Other versions
WO2006010839B1 (fr
WO2006010839A2 (fr
Inventor
Eric Trinquet
Gerard Mathis
Original Assignee
Cis Bio Int
Eric Trinquet
Gerard Mathis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cis Bio Int, Eric Trinquet, Gerard Mathis filed Critical Cis Bio Int
Priority to JP2007517367A priority Critical patent/JP4777981B2/ja
Priority to EP05783888A priority patent/EP1766374A2/fr
Priority to US11/571,331 priority patent/US7872243B2/en
Publication of WO2006010839A2 publication Critical patent/WO2006010839A2/fr
Publication of WO2006010839A3 publication Critical patent/WO2006010839A3/fr
Publication of WO2006010839B1 publication Critical patent/WO2006010839B1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L’invention concerne l’utilisation du phénomène de polarisation de fluorescence, en vue d’améliorer la détection des signaux de fluorescence lors d’un transfert d’énergie non radiatif (FRET). En particulier, l’invention concerne une méthode d’amélioration du rapport signal/bruit de fond dans une mesure de FRET. L’invention concerne également un appareil permettant de mesurer la fluorescence consécutive à un transfert d’énergie entre un composé fluorescent donneur et un composé fluorescent accepteur présents dans un milieu de mesure.
PCT/FR2005/001618 2004-06-28 2005-06-27 Procede d’amelioration de la detection des signaux de fluorescence lors d’un transfert d’energie non radiatif WO2006010839A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2007517367A JP4777981B2 (ja) 2004-06-28 2005-06-27 共鳴エネルギー移動中における蛍光の信号検出を改善する方法
EP05783888A EP1766374A2 (fr) 2004-06-28 2005-06-27 Procede d"amelioration de la detection des signaux de fluorescence lors d"un transfert d"energie non radiatif
US11/571,331 US7872243B2 (en) 2004-06-28 2005-06-27 Method for improving the detection of fluorescence signals during a resonance energy transfer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0407087 2004-06-28
FR0407087A FR2872287B1 (fr) 2004-06-28 2004-06-28 Procede d'amelioration de la detection des signaux de fluorescence lors d'un transfert d'energie non radiatif

Publications (3)

Publication Number Publication Date
WO2006010839A2 WO2006010839A2 (fr) 2006-02-02
WO2006010839A3 true WO2006010839A3 (fr) 2006-06-22
WO2006010839B1 WO2006010839B1 (fr) 2006-08-17

Family

ID=34946931

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2005/001618 WO2006010839A2 (fr) 2004-06-28 2005-06-27 Procede d’amelioration de la detection des signaux de fluorescence lors d’un transfert d’energie non radiatif

Country Status (5)

Country Link
US (1) US7872243B2 (fr)
EP (1) EP1766374A2 (fr)
JP (1) JP4777981B2 (fr)
FR (1) FR2872287B1 (fr)
WO (1) WO2006010839A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2872287B1 (fr) 2004-06-28 2007-03-16 Cis Bio Internat Sa Procede d'amelioration de la detection des signaux de fluorescence lors d'un transfert d'energie non radiatif
US8031338B2 (en) * 2004-12-02 2011-10-04 Vanderbilt University Measuring Forster resonance energy transfer with polarized and depolarized light
EP1866443A4 (fr) 2005-04-04 2009-04-08 Blueshift Biotechnologies Inc Criblage au moyen d'une anisotropie de polarisation dans des emissions de fret
GB0603636D0 (en) * 2006-02-23 2006-04-05 Advanced Sensors Ltd Apparatus for measuring fluorescent material in a liquid
US8269191B2 (en) * 2010-11-05 2012-09-18 The United States Of America As Represented By The Secretary Of The Army System and method for measuring depolarization
JP2014520275A (ja) * 2011-06-23 2014-08-21 コリア リサーチ インスティテュート オブ バイオサイエンス アンド バイオテクノロジー ｉＦＲＥＴ用探針を利用してタンパク質を検出又はイメージ化する顕微鏡装置及びこれを利用したタンパク質の検出又はイメージング方法
CN104487097B (zh) * 2011-12-30 2019-01-18 华盛顿大学商业中心 具有窄带发射的发色聚合物点
US20150289372A1 (en) 2014-04-03 2015-10-08 Yikang Deng Fluorescent conductive fill material for plated through hole structures and methods of defect inspection utilizing the same
CN105466902A (zh) * 2016-01-18 2016-04-06 华南师范大学 一种荧光共振能量转移敏化淬灭转化因子的测量方法
CN105842210B (zh) * 2016-03-23 2018-08-24 南昌大学 基于生物量子点和Au NPs荧光共振能量转移的凝血酶检测方法
CN106442455B (zh) * 2016-11-23 2019-01-08 华南师范大学 一种基于双通道荧光强度同时探测的荧光共振能量转移效率的快速测量方法
CN112763463A (zh) * 2020-11-05 2021-05-07 北京工业大学 一种提高回音壁模式微腔传感灵敏度的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002098638A (ja) * 2000-09-26 2002-04-05 Shimadzu Corp 蛍光偏光度測定装置
US20030058442A1 (en) * 2000-11-17 2003-03-27 Gyozo Garab Method and apparatus for determining the polarization properties of light emitted, reflected or transmitted by a material using a laser scanning microscope

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030228703A1 (en) * 2002-04-05 2003-12-11 The Regents Of The University Of Michigan Fluorescence resonance energy transfer quantitation and stoichiometry in living cells
JP4214206B2 (ja) * 2002-12-10 2009-01-28 独立行政法人理化学研究所 Ｆｒｅｔを利用した蛍光指示薬
FR2872287B1 (fr) 2004-06-28 2007-03-16 Cis Bio Internat Sa Procede d'amelioration de la detection des signaux de fluorescence lors d'un transfert d'energie non radiatif

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002098638A (ja) * 2000-09-26 2002-04-05 Shimadzu Corp 蛍光偏光度測定装置
US20030058442A1 (en) * 2000-11-17 2003-03-27 Gyozo Garab Method and apparatus for determining the polarization properties of light emitted, reflected or transmitted by a material using a laser scanning microscope

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
COHEN-KASHI M: "Fluorescence resonance energy transfers measurements on cell surfaces via fluorescence polarization", BIOPHYSICAL JOURNAL, vol. 83, 2002, pages 1395 - 1402, XP002328968 *
GAUTIER I ET AL: "Homo-FRET microscopy in living cells to measure monomer-dimer transition of GFP-tagged proteins", BIOPHYSICAL JOURNAL, NEW YORK, US, US, vol. 80, no. 6, June 2001 (2001-06-01), pages 3000 - 3008, XP002977865, ISSN: 0006-3495 *
GORDON G W: "Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy", BIOPHYSICAL JOURNAL, vol. 74, 1998, pages 2702 - 2713, XP000990953 *
MOENS P D J ET AL: "Detection of tryptophan to tryptophan energy transfer in proteins", THE PROTEIN JOURNAL, vol. 23, no. 1, 1 January 2004 (2004-01-01), pages 79 - 83, XP002328973 *
PATENT ABSTRACTS OF JAPAN vol. 2002, no. 08 5 August 2002 (2002-08-05) *
See also references of EP1766374A2 *
XIA Z AND LIU Y: "Reliable and global measurement of fluorescence resonance energy transfer using fluorescence microscopes", BIOPHYSICAL JOURNAL, vol. 81, 2001, pages 2395 - 2402, XP002328970 *

Also Published As

Publication number Publication date
EP1766374A2 (fr) 2007-03-28
JP4777981B2 (ja) 2011-09-21
FR2872287A1 (fr) 2005-12-30
US20090294691A1 (en) 2009-12-03
US7872243B2 (en) 2011-01-18
FR2872287B1 (fr) 2007-03-16
WO2006010839B1 (fr) 2006-08-17
WO2006010839A2 (fr) 2006-02-02
JP2008504517A (ja) 2008-02-14

Similar Documents

Publication Publication Date Title
WO2006010839A3 (fr) Procede d’amelioration de la detection des signaux de fluorescence lors d’un transfert d’energie non radiatif
WO2010077649A3 (fr) Appareil et procédé d'estimation de compositions de terrain en fond de trou
TW200704778A (en) Screening using polarization anisotropy in fret emissions
WO2007086840A3 (fr) Système et procédé de résonance magnétique pour détecter et confirmer des analytes
WO2008057613A3 (fr) Système à résonance magnétique et procédé de détection et de confirmation d'analytes
WO2008150979A9 (fr) Procédé et appareil pour diagraphie de sondage rmn à rapport signal sur bruit élevé
WO2005089409A3 (fr) Constructions de capteurs et procedes de detection associes
WO2006130732A3 (fr) Procede de mesure de la sensibilite d'un recepteur de signal de paquet de donnees
WO2004099432A3 (fr) Identification de biomarqueurs permettant de detecter la presence d'un cancer du pancreas
WO2006020579A3 (fr) Procede d'identification rapide de micro-organismes
WO2006073811A3 (fr) Système de détection par fluorescence
WO2004013609A8 (fr) Utilisation de biomarqueurs pour detecter un cancer de l'ovaire
WO2008135862A3 (fr) Dispositifs et procédés pour détecter des analysats
GB2449043A (en) Water soluble conjugates for electrochemical detection
EP2987866A3 (fr) Procédé et kit de mesure à super-haute sensibilité de protéines et acides nucléiques et nouveau substrat d'enzyme
WO2007026099A3 (fr) Procede pour la mise en evidence d'un processus biologique par mesure d'un fret
WO2006002433A3 (fr) Procedes et reactifs pour la detection de melanome
AU2002221848A1 (en) Dye pair for fluorescence resonance energy transfer (fret) measurements
WO2006002243A3 (fr) Procedes de profilage d'oligosaccharides pour la detection du cancer
WO2005118836A3 (fr) Essai d'activite de prolyl hydroxylase de hif
WO2007035864A3 (fr) Standards, procedes et ensembles d'electrophorese
GB9901072D0 (en) Methods for detecting changes to a macromolecular component of a cell
WO2006008172A3 (fr) Procede et appareil d'estimation d'une distribution de permeabilite lors d'un essai de puits
WO2005031005A3 (fr) Compositions, methodes et kits de detection de la presence de trichomonas vaginalis dans un echantillon d'essai
WO2009055382A3 (fr) Dosages immunologiques en une étape présentant une sensibilité et une spécificité accrues

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KM KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NG NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2007517367

Country of ref document: JP

REEP Request for entry into the european phase

Ref document number: 2005783888

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2005783888

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2005783888

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 11571331

Country of ref document: US