JP4625318B2 - 相同組換え頻度が上昇した形質転換菌 - Google Patents

相同組換え頻度が上昇した形質転換菌 Download PDF

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JP4625318B2
JP4625318B2 JP2004353026A JP2004353026A JP4625318B2 JP 4625318 B2 JP4625318 B2 JP 4625318B2 JP 2004353026 A JP2004353026 A JP 2004353026A JP 2004353026 A JP2004353026 A JP 2004353026A JP 4625318 B2 JP4625318 B2 JP 4625318B2
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gene
strain
dna
homologous recombination
transformed bacterium
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JP2004353026A
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English (en)
Japanese (ja)
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JP2006158269A5 (enExample
JP2006158269A (ja
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理 高橋
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Noda Institute for Scientific Research
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Noda Institute for Scientific Research
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Priority to JP2004353026A priority Critical patent/JP4625318B2/ja
Priority to US11/292,071 priority patent/US8569038B2/en
Priority to EP05026494.4A priority patent/EP1666601B2/en
Priority to DK05026494.4T priority patent/DK1666601T4/da
Priority to DE602005019966T priority patent/DE602005019966D1/de
Publication of JP2006158269A publication Critical patent/JP2006158269A/ja
Publication of JP2006158269A5 publication Critical patent/JP2006158269A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2004353026A 2004-12-06 2004-12-06 相同組換え頻度が上昇した形質転換菌 Expired - Lifetime JP4625318B2 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2004353026A JP4625318B2 (ja) 2004-12-06 2004-12-06 相同組換え頻度が上昇した形質転換菌
US11/292,071 US8569038B2 (en) 2004-12-06 2005-12-02 Transformant having an increased frequency of homologous recombination
DE602005019966T DE602005019966D1 (de) 2004-12-06 2005-12-05 Transformant mit erhöhter Frequenz homologer Rekombination
DK05026494.4T DK1666601T4 (da) 2004-12-06 2005-12-05 Transformant med forøget frekvens af homolog rekombination
EP05026494.4A EP1666601B2 (en) 2004-12-06 2005-12-05 Transformant having an increased frequency of homologous recombination

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2004353026A JP4625318B2 (ja) 2004-12-06 2004-12-06 相同組換え頻度が上昇した形質転換菌

Publications (3)

Publication Number Publication Date
JP2006158269A JP2006158269A (ja) 2006-06-22
JP2006158269A5 JP2006158269A5 (enExample) 2007-06-28
JP4625318B2 true JP4625318B2 (ja) 2011-02-02

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ID=35953872

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JP2004353026A Expired - Lifetime JP4625318B2 (ja) 2004-12-06 2004-12-06 相同組換え頻度が上昇した形質転換菌

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Country Link
US (1) US8569038B2 (enExample)
EP (1) EP1666601B2 (enExample)
JP (1) JP4625318B2 (enExample)
DE (1) DE602005019966D1 (enExample)
DK (1) DK1666601T4 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007222055A (ja) * 2006-02-22 2007-09-06 Noda Inst For Scient Res 染色体領域欠失株の作製方法
JP2008263927A (ja) * 2007-04-25 2008-11-06 Noda Inst For Scient Res 染色体大領域の欠失方法
JP6081245B2 (ja) 2012-04-07 2017-02-15 キッコーマン株式会社 麹菌染色体における任意領域の重複転座の作製方法
DK3234160T3 (da) * 2014-12-16 2019-12-02 Danisco Us Inc Sammensætninger og fremgangsmåder til hjælper-stamme-medieret svampegenommodifikation
JP6445884B2 (ja) * 2015-01-30 2018-12-26 公益財団法人野田産業科学研究所 抗菌物質
JP6757341B2 (ja) * 2018-01-26 2020-09-16 東洋紡株式会社 アスペルギルス属微生物が生産するタンパク質のｎ型糖鎖構造を改変する方法
JP6599583B1 (ja) * 2018-11-05 2019-10-30 キッコーマン株式会社 多重遺伝子破壊アスペルギルス属微生物及びその製造方法
CN114621882A (zh) * 2020-12-10 2022-06-14 中国科学院大连化学物理研究所 一种具有高同源重组效率的毕赤酵母菌株及其应用
CN113717914B (zh) * 2021-09-23 2022-08-02 陕西海斯夫生物工程有限公司 高效同源重组的拟无枝酸菌工程菌株、其构建方法及应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0696319T3 (da) * 1993-04-30 2003-05-19 Novozymes As Enzym, der udviser pectinmethylesterase-aktivitet
JP2002529705A (ja) * 1998-10-29 2002-09-10 コンネクス・ゲーエムベーハー 糞便中の耐酸性微生物を検出するための新規方法
US6569681B1 (en) 2000-03-14 2003-05-27 Transkaryotic Therapies, Inc. Methods of improving homologous recombination
KR20020026456A (ko) * 2000-04-13 2002-04-10 에말파브 마크 아론 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝
EP1217074A1 (en) * 2000-12-22 2002-06-26 Universiteit Leiden Nucleic acid integration in eukaryotes
EP1384782A4 (en) 2001-03-30 2005-05-25 Nat Inst Of Advanced Ind Scien DETECTION PROCEDURE FOR THE EXPRESSION OF AN ASPERGILLUS GENE
JP4050712B2 (ja) * 2004-02-27 2008-02-20 独立行政法人科学技術振興機構 相同組換えを行わせる方法
DE602005023427D1 (de) * 2004-04-02 2010-10-21 Dsm Ip Assets Bv Filamentöse pilzmutanten mit verbesserter homologer rekombinationseffizienz

Also Published As

Publication number Publication date
US8569038B2 (en) 2013-10-29
US20060183233A1 (en) 2006-08-17
EP1666601B1 (en) 2010-03-17
DE602005019966D1 (de) 2010-04-29
DK1666601T4 (da) 2014-08-18
EP1666601A1 (en) 2006-06-07
DK1666601T3 (da) 2010-06-21
JP2006158269A (ja) 2006-06-22
EP1666601B2 (en) 2014-07-23

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