DE602005019966D1 - Transformant mit erhöhter Frequenz homologer Rekombination - Google Patents
Transformant mit erhöhter Frequenz homologer RekombinationInfo
- Publication number
- DE602005019966D1 DE602005019966D1 DE602005019966T DE602005019966T DE602005019966D1 DE 602005019966 D1 DE602005019966 D1 DE 602005019966D1 DE 602005019966 T DE602005019966 T DE 602005019966T DE 602005019966 T DE602005019966 T DE 602005019966T DE 602005019966 D1 DE602005019966 D1 DE 602005019966D1
- Authority
- DE
- Germany
- Prior art keywords
- transformant
- homologous recombination
- increased frequency
- frequency
- increased
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/111—Antisense spanning the whole gene, or a large part of it
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004353026A JP4625318B2 (ja) | 2004-12-06 | 2004-12-06 | 相同組換え頻度が上昇した形質転換菌 |
Publications (1)
Publication Number | Publication Date |
---|---|
DE602005019966D1 true DE602005019966D1 (de) | 2010-04-29 |
Family
ID=35953872
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE602005019966T Active DE602005019966D1 (de) | 2004-12-06 | 2005-12-05 | Transformant mit erhöhter Frequenz homologer Rekombination |
Country Status (5)
Country | Link |
---|---|
US (1) | US8569038B2 (de) |
EP (1) | EP1666601B2 (de) |
JP (1) | JP4625318B2 (de) |
DE (1) | DE602005019966D1 (de) |
DK (1) | DK1666601T4 (de) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007222055A (ja) * | 2006-02-22 | 2007-09-06 | Noda Inst For Scient Res | 染色体領域欠失株の作製方法 |
JP2008263927A (ja) * | 2007-04-25 | 2008-11-06 | Noda Inst For Scient Res | 染色体大領域の欠失方法 |
JP6081245B2 (ja) | 2012-04-07 | 2017-02-15 | キッコーマン株式会社 | 麹菌染色体における任意領域の重複転座の作製方法 |
JP6445884B2 (ja) * | 2015-01-30 | 2018-12-26 | 公益財団法人野田産業科学研究所 | 抗菌物質 |
JP6599583B1 (ja) * | 2018-11-05 | 2019-10-30 | キッコーマン株式会社 | 多重遺伝子破壊アスペルギルス属微生物及びその製造方法 |
CN114621882A (zh) * | 2020-12-10 | 2022-06-14 | 中国科学院大连化学物理研究所 | 一种具有高同源重组效率的毕赤酵母菌株及其应用 |
CN113717914B (zh) * | 2021-09-23 | 2022-08-02 | 陕西海斯夫生物工程有限公司 | 高效同源重组的拟无枝酸菌工程菌株、其构建方法及应用 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE231914T1 (de) * | 1993-04-30 | 2003-02-15 | Novozymes As | Ein enzym mit pektin methylesteraseaktivität |
DE59913716D1 (de) * | 1998-10-29 | 2006-09-07 | Dakocytomation Denmark As | Nachweis von säure-resistenten mikroorganismen im stuhl |
US6569681B1 (en) * | 2000-03-14 | 2003-05-27 | Transkaryotic Therapies, Inc. | Methods of improving homologous recombination |
KR20020026456A (ko) * | 2000-04-13 | 2002-04-10 | 에말파브 마크 아론 | 사상균에서 발현된 dna 라이브러리의 고산출량 스크리닝 |
EP1217074A1 (de) * | 2000-12-22 | 2002-06-26 | Universiteit Leiden | Integration von Nukleinsäure in Eukaryoten |
EP1384782A4 (de) | 2001-03-30 | 2005-05-25 | Nat Inst Of Advanced Ind Scien | Nachweisverfahren für die expression eines aspergillus-gens |
JP4050712B2 (ja) * | 2004-02-27 | 2008-02-20 | 独立行政法人科学技術振興機構 | 相同組換えを行わせる方法 |
WO2005095624A2 (en) * | 2004-04-02 | 2005-10-13 | Dsm Ip Assets B.V. | Filamentous fungal mutants with improved homologous recombination efficiency |
-
2004
- 2004-12-06 JP JP2004353026A patent/JP4625318B2/ja active Active
-
2005
- 2005-12-02 US US11/292,071 patent/US8569038B2/en active Active
- 2005-12-05 DE DE602005019966T patent/DE602005019966D1/de active Active
- 2005-12-05 EP EP05026494.4A patent/EP1666601B2/de active Active
- 2005-12-05 DK DK05026494.4T patent/DK1666601T4/da active
Also Published As
Publication number | Publication date |
---|---|
EP1666601A1 (de) | 2006-06-07 |
JP4625318B2 (ja) | 2011-02-02 |
DK1666601T4 (da) | 2014-08-18 |
DK1666601T3 (da) | 2010-06-21 |
JP2006158269A (ja) | 2006-06-22 |
US8569038B2 (en) | 2013-10-29 |
EP1666601B2 (de) | 2014-07-23 |
US20060183233A1 (en) | 2006-08-17 |
EP1666601B1 (de) | 2010-03-17 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8363 | Opposition against the patent | ||
R082 | Change of representative |
Ref document number: 1666601 Country of ref document: EP Representative=s name: STREHL, SCHUEBEL-HOPF & PARTNER, 80538 MUENCHEN, D |