JP4422797B2 - ペプチドおよび核酸のクロマトグラフィー的分離法、ならびに新規な高アフィニティーイオン交換マトリクス - Google Patents

ペプチドおよび核酸のクロマトグラフィー的分離法、ならびに新規な高アフィニティーイオン交換マトリクス Download PDF

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Publication number
JP4422797B2
JP4422797B2 JP52927797A JP52927797A JP4422797B2 JP 4422797 B2 JP4422797 B2 JP 4422797B2 JP 52927797 A JP52927797 A JP 52927797A JP 52927797 A JP52927797 A JP 52927797A JP 4422797 B2 JP4422797 B2 JP 4422797B2
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chohch
group
anion exchanger
hydrogen
groups
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Expired - Lifetime
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JP52927797A
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Japanese (ja)
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JP2000504625A5 (cg-RX-API-DMAC7.html
JP2000504625A (ja
Inventor
バリィルンド,ロルフ
バリィストレム,ヤン
ベレヴ,マコネン
セデルバリィ,レナート
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Cytiva Sweden AB
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GE Healthcare Bio Sciences Corp
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361Ion-exchange
    • B01D15/363Anion-exchange
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/04Processes using organic exchangers
    • B01J41/07Processes using organic exchangers in the weakly basic form
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20Anion exchangers for chromatographic processes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/18Ion-exchange chromatography
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00Aspects relating to sorbent materials
    • B01J2220/50Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54Sorbents specially adapted for analytical or investigative chromatography

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
JP52927797A 1996-02-19 1997-02-14 ペプチドおよび核酸のクロマトグラフィー的分離法、ならびに新規な高アフィニティーイオン交換マトリクス Expired - Lifetime JP4422797B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9600590-5 1996-02-19
SE9600590A SE9600590D0 (sv) 1996-02-19 1996-02-19 Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris
PCT/SE1997/000237 WO1997029825A1 (en) 1996-02-19 1997-02-14 Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix

Publications (3)

Publication Number Publication Date
JP2000504625A JP2000504625A (ja) 2000-04-18
JP2000504625A5 JP2000504625A5 (cg-RX-API-DMAC7.html) 2004-11-18
JP4422797B2 true JP4422797B2 (ja) 2010-02-24

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JP52927797A Expired - Lifetime JP4422797B2 (ja) 1996-02-19 1997-02-14 ペプチドおよび核酸のクロマトグラフィー的分離法、ならびに新規な高アフィニティーイオン交換マトリクス

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US (1) US6090288A (cg-RX-API-DMAC7.html)
EP (1) EP0888157B1 (cg-RX-API-DMAC7.html)
JP (1) JP4422797B2 (cg-RX-API-DMAC7.html)
AT (1) ATE267037T1 (cg-RX-API-DMAC7.html)
AU (1) AU713635B2 (cg-RX-API-DMAC7.html)
DE (1) DE69729182T2 (cg-RX-API-DMAC7.html)
SE (1) SE9600590D0 (cg-RX-API-DMAC7.html)
WO (1) WO1997029825A1 (cg-RX-API-DMAC7.html)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
KR100463475B1 (ko) 1995-06-08 2005-06-22 로셰 디아그노스틱스 게엠베하 자기성피그먼트
DE19520398B4 (de) 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
SE9700769D0 (sv) 1997-03-04 1997-03-04 Pharmacia Biotech Ab Matriser för separation och separation som utnyttjar matriserna
DE19736366A1 (de) * 1997-08-21 1999-02-25 Ernst Prof Dr Bayer Verfahren zur Isolierung von anionischen organischen Verbindungen aus wäßrigen Systemen mit kationischen Polymernanopartikeln
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
BR9815569A (pt) * 1997-12-06 2001-10-09 Dna Res Instr Ltd Método para extração de biomoléculas de material biológico
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
SE9802214D0 (sv) * 1998-06-18 1998-06-18 Amersham Pharm Biotech Ab Jonbytare och användning därav
WO2000034521A1 (en) * 1998-12-08 2000-06-15 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
EP1147226B1 (en) * 1999-01-27 2013-01-23 Folim G. Halaka Materials and methods for the purification of polyelectrolytes
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
SE9904197D0 (sv) * 1999-11-22 1999-11-22 Amersham Pharm Biotech Ab A method for anion exchange adsorption on matrices carrying mixed mode ligands
GB9927904D0 (en) * 1999-11-25 2000-01-26 Amersham Pharm Biotech Ab A method fro obtaining a nucleic acid variant
SE9904272D0 (sv) * 1999-11-25 1999-11-25 Amersham Pharm Biotech Ab A method for selective removal of a substance from samples containing compounds having nucleic acid structure
EP1257562B1 (en) * 2000-02-08 2006-11-29 Upfront Chromatography A/S Novel resin materials and their use for recovering proteins or peptides
US20030066336A1 (en) * 2000-12-19 2003-04-10 Showa Denko K.K. Method for measuring anions
US20020194901A1 (en) * 2000-12-19 2002-12-26 Showa Denko K.K. Porous polymer particle, alkali-resistant anion exchanger, producing method thereof, column for ion chromatography, and method for measuring anions
SE0004932D0 (sv) * 2000-12-31 2000-12-31 Apbiotech Ab A method for mixed mode adsorption and mixed mode adsorbents
DE60202240T2 (de) * 2001-05-21 2005-12-15 Fuji Photo Film Co., Ltd., Minami-Ashigara Vorrichtung zur Durchführung biochemischer Analysen und Verfahren zu ihrer Herstellung
SE0103084D0 (sv) * 2001-09-14 2001-09-14 Amersham Pharm Biotech Ab Generation of ion exchange media
GB0127809D0 (en) * 2001-11-20 2002-01-09 Dna Res Innovations Ltd Purification of nucleic acid
CN1617938A (zh) 2002-01-16 2005-05-18 戴诺生物技术有限公司 从单个样品中分离核酸和蛋白质的方法
US20040029143A1 (en) * 2002-02-28 2004-02-12 Jeffrey Van Ness Cationic polyelectrolytes in biomolecule purification and analysis
US20060035224A1 (en) * 2002-03-21 2006-02-16 Johansen Jack T Purification methods for oligonucleotides and their analogs
GB0207975D0 (en) * 2002-04-05 2002-05-15 Genovision As Isolating nucleic acid
GB0212826D0 (en) * 2002-05-31 2002-07-10 Dna Res Innovations Ltd Materials and methods relating to polyions and substance delivery
US6989442B2 (en) * 2002-07-12 2006-01-24 Sirna Therapeutics, Inc. Deprotection and purification of oligonucleotides and their derivatives
DE602004018616D1 (de) * 2003-03-05 2009-02-05 Ge Healthcare Bio Sciences Ab Verfahren zur herstellung von multimodalen anionenaustauschliganden
SE0302911D0 (sv) * 2003-10-31 2003-10-31 Amersham Biosciences Ab Novel separation matrix
US20050106589A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Compositions and methods for releasing nucleic acids from solid phase binding materials
SE0303532D0 (sv) * 2003-12-23 2003-12-23 Amersham Biosciences Ab Purification of immunoglobulins
SG150501A1 (en) * 2004-02-05 2009-03-30 Millipore Corp Porous adsorptive or chromatographic media
EP1693108A1 (en) * 2004-12-04 2006-08-23 MERCK PATENT GmbH Mixed-modal anion-exchange type separation material
KR100668337B1 (ko) 2005-05-21 2007-01-12 삼성전자주식회사 단백질에 비하여 핵산에 대한 결합력이 선택적으로 높은pH 의존성 이온 교환물질, 그가 고정화되어 있는 고체기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법
CN102417557B (zh) 2005-08-03 2015-03-11 默克专利股份公司 亲水交联聚合物
EP1754534A1 (de) 2005-08-03 2007-02-21 MERCK PATENT GmbH Hydrophiles vernetztes Polymer
GB2445441B (en) * 2006-09-26 2010-06-30 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2443505B (en) 2006-09-26 2008-12-31 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2445442A (en) 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
DE202008017854U1 (de) 2007-08-14 2010-08-19 Millipore Corp., Billerica Mittel zur Ionenaustausch-Membranchromatographie auf der Basis von primären polymerischen Aminen, Sorptionsvorrichtung mit diesem Mittel, Chromatographieverfahren und Reinigungsverfahren unter dessen Verwendung
US9433922B2 (en) 2007-08-14 2016-09-06 Emd Millipore Corporation Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same
US20090130738A1 (en) * 2007-11-19 2009-05-21 Mikhail Kozlov Media for membrane ion exchange chromatography
EP2250182B1 (en) * 2008-02-07 2015-12-02 GE Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
WO2009126603A1 (en) 2008-04-08 2009-10-15 Bio-Rad Laboratories, Inc. Chromatography purification of antibodies
US20110065900A1 (en) * 2008-05-30 2011-03-17 Ge Healthcare Bio-Science Ab Separation method utilizing polyallylamine ligands
US9481881B2 (en) 2011-01-12 2016-11-01 Sekisui Medical Co., Ltd. Eluent for ion-exchange chromatography, and method of analyzing nucleic acid chains
JP6061381B2 (ja) 2011-01-12 2017-01-18 積水メディカル株式会社 一塩基多型の検出方法
CN105911183B (zh) * 2011-02-10 2019-03-22 积水医疗株式会社 离子交换色谱法用填充剂以及核酸链的分离检测方法
US9669402B2 (en) * 2012-03-08 2017-06-06 Bio-Rad Laboratories, Inc. Anionic exchange-hydrophobic mixed mode
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
EP2812091B1 (en) 2012-09-17 2021-03-10 W.R. Grace & CO. - CONN. Chromatography media and devices
EP2811983B1 (en) * 2012-09-17 2019-05-01 W.R. Grace & CO. - CONN. Functionalized particulate support material and methods of making and using the same
EP2925432A4 (en) * 2012-11-29 2016-08-31 Affigel Ab SUPER-HYDROXYLATED ADSORBENTS AND USES THEREOF
ES2887110T3 (es) 2014-01-16 2021-12-21 Grace W R & Co Medios para cromatografía de afinidad y dispositivos para cromatografía
JP6702587B2 (ja) * 2014-03-14 2020-06-03 ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ 生物粒子の精製用分離マトリックス
PL3137209T3 (pl) 2014-05-02 2023-01-02 W.R. Grace & Co. - Conn. Funkcjonalizowany materiał nośnikowy i sposoby wytwarzania oraz stosowania funkcjonalizowanego materiału nośnikowego
US10280195B2 (en) 2014-05-28 2019-05-07 Agency For Science, Technology And Research Virus reduction method
US10526287B2 (en) 2015-04-23 2020-01-07 Constellation Pharmaceuticals, Inc. LSD1 inhibitors and uses thereof
KR102566292B1 (ko) 2015-06-05 2023-08-10 더블유.알. 그레이스 앤드 캄파니-콘. 흡착성 바이오프로세싱 정화제와 이의 제조 및 사용 방법
CN109715648A (zh) * 2016-09-15 2019-05-03 克拉维格两合股份有限公司 聚合网状物用于大分子纯化的用途
TWI753037B (zh) 2016-10-26 2022-01-21 美商星座製藥公司 Lsd1抑制劑及其用途
US11123656B2 (en) * 2017-06-22 2021-09-21 Showa Denko K.K. Separation/analysis method for mixture of oligonucleotides
EP3749697A4 (en) 2018-02-05 2021-11-03 Bio-Rad Laboratories, Inc. CHROMATOGRAPHY RESIN WITH LIGAND MIXED MODE ANIONIC / HYDROPHOBIC EXCHANGE
US12343702B2 (en) 2018-03-08 2025-07-01 Bio-Rad Laboratories, Inc. Anionic exchange-hydrophobic mixed mode chromatography resin
WO2021046284A1 (en) 2019-09-05 2021-03-11 Bio-Rad Laboratories, Inc. Anionic exchange-hydrophobic mixed mode chromatography resins
EP4070102A1 (en) 2019-12-05 2022-10-12 Waters Technologies Corporation Polyphosphonic acids for improving recovery and minimizing system loss
EP4182460A1 (en) * 2020-07-14 2023-05-24 Waters Technologies Corporation Kits and methods useful for separating oligonucleotides from matrix components

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4767670A (en) * 1987-01-21 1988-08-30 E. I. Du Pont De Nemours And Company Chromatographic supports for separation of oligonucleotides
SE8902315D0 (sv) * 1989-06-27 1989-06-27 Pharmacia Ab Anjonbytare
US5087359A (en) * 1989-08-07 1992-02-11 J. T. Baker Inc. Quaternized PEI silica solid supports for chromatography
ATE103507T1 (de) * 1989-08-07 1994-04-15 Baker J T Inc Feste traeger aus quaternisierter pei-kieselsaeure fuer die chromatographie.
SE9004129D0 (sv) * 1990-12-21 1990-12-21 Pharmacia Lkb Biotech Anion exchanger

Also Published As

Publication number Publication date
EP0888157A1 (en) 1999-01-07
AU713635B2 (en) 1999-12-09
ATE267037T1 (de) 2004-06-15
US6090288A (en) 2000-07-18
EP0888157B1 (en) 2004-05-19
SE9600590D0 (sv) 1996-02-19
DE69729182D1 (de) 2004-06-24
AU1818097A (en) 1997-09-02
DE69729182T2 (de) 2005-01-27
WO1997029825A1 (en) 1997-08-21
JP2000504625A (ja) 2000-04-18

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