JP4372341B2 - リニアービーコン（ＬｉｎｅａｒＢｅａｃｏｎｓ）に関する方法、キットおよび組成物 - Google Patents
リニアービーコン（ＬｉｎｅａｒＢｅａｃｏｎｓ）に関する方法、キットおよび組成物 Download PDFInfo
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- JP4372341B2 JP4372341B2 JP2000517989A JP2000517989A JP4372341B2 JP 4372341 B2 JP4372341 B2 JP 4372341B2 JP 2000517989 A JP2000517989 A JP 2000517989A JP 2000517989 A JP2000517989 A JP 2000517989A JP 4372341 B2 JP4372341 B2 JP 4372341B2
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6485901B1 (en) * | 1997-10-27 | 2002-11-26 | Boston Probes, Inc. | Methods, kits and compositions pertaining to linear beacons |
| US6361942B1 (en) | 1998-03-24 | 2002-03-26 | Boston Probes, Inc. | Method, kits and compositions pertaining to detection complexes |
| US6287772B1 (en) * | 1998-04-29 | 2001-09-11 | Boston Probes, Inc. | Methods, kits and compositions for detecting and quantitating target sequences |
| ATE449868T1 (de) | 1998-04-29 | 2009-12-15 | Boston Probes Inc | Methoden, testsätze und kompositionen zur detektion und quantifizierung von zielsequenzen |
| GB9815933D0 (en) | 1998-07-23 | 1998-09-23 | Secr Defence | Detection method |
| US6441152B1 (en) | 1998-12-08 | 2002-08-27 | Boston Probes, Inc. | Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices |
| US6692965B1 (en) * | 1999-11-23 | 2004-02-17 | Chromocell Corporation | Isolation of living cells and preparation of cell lines based on detection and quantification of preselected cellular ribonucleic acid sequences |
| US20040081959A9 (en) * | 1999-12-08 | 2004-04-29 | Epoch Biosciences, Inc. | Fluorescent quenching detection reagents and methods |
| US6727356B1 (en) | 1999-12-08 | 2004-04-27 | Epoch Pharmaceuticals, Inc. | Fluorescent quenching detection reagents and methods |
| GB2359625B (en) | 1999-12-10 | 2004-10-20 | Molecular Light Tech Res Ltd | Monitoring oligonucleotide binding process using chemiluminescence quenching |
| US6579680B2 (en) | 2000-02-28 | 2003-06-17 | Corning Incorporated | Method for label-free detection of hybridized DNA targets |
| US6887664B2 (en) | 2000-06-06 | 2005-05-03 | Applera Corporation | Asynchronous primed PCR |
| EP1295941B1 (en) * | 2000-06-27 | 2009-12-16 | National Institute of Advanced Industrial Science and Technology | Novel nucleic acid probes and method of assaying nucleic acid by using the same |
| US20020090626A1 (en) | 2000-09-26 | 2002-07-11 | Hyldig-Nielsen Jens J. | Probes, probe sets, methods and kits pertaining to the detection, identification and/or enumeration of bacteria |
| WO2002057493A2 (en) | 2000-12-15 | 2002-07-25 | Boston Probes, Inc. | Methods for determining organisms |
| JP4808365B2 (ja) * | 2001-03-02 | 2011-11-02 | ユニバーシティ オブ ピッツバーグ オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション | Ｐｃｒ法 |
| EP1748078B1 (en) | 2001-03-09 | 2011-01-19 | Boston Probes, Inc. | Methods, kits and compositions of combination oligomers |
| AU2007200335B2 (en) * | 2001-03-09 | 2009-01-22 | Boston Probes, Inc. | Methods, kits and compositions pertaining to combination oligomers and libraries for their preparation |
| AU2002257284A1 (en) | 2001-05-18 | 2002-12-03 | Boston Probes, Inc. | Pna probes, probe sets, methods and kits pertaining to the detection of candida |
| US7297494B2 (en) * | 2001-06-25 | 2007-11-20 | Georgia Tech Research Corporation | Activatable probes and methods for in vivo gene detection |
| EP1432826B1 (en) | 2001-09-24 | 2012-12-12 | Life Technologies Corporation | Methods, kits and compositions pertaining to the suppression of detectable probe binding to randomly distributed repeat sequences in genomic nucleic acid |
| EP1444365A4 (en) * | 2001-10-25 | 2005-07-20 | Gorilla Genomics Inc | ASYMMETRIC PCR WITH NUCLEASE-FREE POLYMERASE OR NUCLEASERESISTENT MOLECULAR BEACONS |
| US8017758B2 (en) * | 2002-03-21 | 2011-09-13 | Boston Probes, Inc. | PNA oligomers, oligomer sets, methods and kits pertaining to the detection of Bacillus anthracis |
| US20030211509A1 (en) * | 2002-03-26 | 2003-11-13 | Wiley Steven R. | TNF-delta ligand and uses thereof |
| EP1509624A2 (en) * | 2002-05-31 | 2005-03-02 | Secretary, Department Of Atomic Energy | Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands |
| EP1575990B1 (en) | 2002-09-08 | 2009-11-18 | Applera Corporation | Solid phase bound PNA-dimers and their synthesis |
| EP1556325A4 (en) * | 2002-09-20 | 2007-09-19 | Integrated Dna Tech Inc | ANTHRAQUINONE-BASED FLUORESCENCE EXTINGUISHING DYES AND METHODS OF MAKING AND USING SAME |
| US20040259132A1 (en) * | 2002-11-22 | 2004-12-23 | Henrik Stender | Peptide nucleic acid probes for analysis of pseudomonas (sensu stricto) |
| EP2194147B1 (en) | 2002-12-04 | 2015-07-22 | Applied Biosystems, LLC | Multiplex amplification of polynucleotides |
| BRPI0410780A (pt) * | 2003-05-20 | 2006-06-27 | Investigen Inc | sistema para detectar polinucleotìdeos |
| JP2007531496A (ja) * | 2003-07-11 | 2007-11-08 | サロメッド，インコーポレイテッド | 病原体を検出するためのマルチプレックス分子ビーコンアッセイ |
| JPWO2005012571A1 (ja) * | 2003-07-30 | 2007-09-27 | 財団法人理工学振興会 | 部分二重鎖型核酸分子 |
| US7417726B2 (en) * | 2003-09-19 | 2008-08-26 | Applied Biosystems Inc. | Normalization of data using controls |
| WO2005029041A2 (en) * | 2003-09-19 | 2005-03-31 | Applera Corporation | High density sequence detection methods and apparatus |
| US7570443B2 (en) | 2003-09-19 | 2009-08-04 | Applied Biosystems, Llc | Optical camera alignment |
| WO2005049849A2 (en) | 2003-11-14 | 2005-06-02 | Integrated Dna Technologies, Inc. | Fluorescence quenching azo dyes, their methods of preparation and use |
| US20050123975A1 (en) * | 2003-11-19 | 2005-06-09 | Applera Corporation | Methods for determining the degradation state or concentration of nucleic acids |
| JP2007534308A (ja) * | 2003-11-19 | 2007-11-29 | アレロジック・バイオサイエンシズ・コーポレーション | 複数のフルオロフォアで標識したオリゴヌクレオチド |
| KR20060129469A (ko) * | 2004-02-18 | 2006-12-15 | 크로모셀 코포레이션 | 신호 프로브를 이용하는 방법 및 물질 |
| EP1721011A1 (en) | 2004-03-01 | 2006-11-15 | Applera Corporation | Methods, compositions and kits for use in polynucleotide amplification |
| US20050255485A1 (en) * | 2004-05-14 | 2005-11-17 | Livak Kenneth J | Detection of gene duplications |
| EP2290070B1 (en) | 2004-05-28 | 2015-03-25 | Asuragen, Inc. | Methods and compositions involving microRNA |
| US20060003337A1 (en) * | 2004-06-30 | 2006-01-05 | John Brandis | Detection of small RNAS |
| WO2006034387A1 (en) | 2004-09-21 | 2006-03-30 | Applera Corporation | TWO-COLOR REAL-TIME/END-POINT QUANTITATION OF MICRORNAS (miRNAs) |
| ES2534302T3 (es) | 2004-11-12 | 2015-04-21 | Asuragen, Inc. | Procedimientos y composiciones que implican miARN y moléculas inhibidoras de miARN |
| JP2008541698A (ja) * | 2005-05-03 | 2008-11-27 | アンサンブル ディスカバリー コーポレイション | 核酸検出のためのターンオーバープローブおよびその使用 |
| EP1907560B1 (en) * | 2005-05-20 | 2013-01-23 | Integrated DNA Technologies, Inc. | Compounds and methods for labeling oligonucleotides |
| DK2236616T3 (da) * | 2005-06-02 | 2014-01-13 | Advandx Inc | Peptid-nukleinsyreprober til analyse af mikroorganismer |
| US9677123B2 (en) | 2006-03-15 | 2017-06-13 | Siemens Healthcare Diagnostics Inc. | Degenerate nucleobase analogs |
| AU2007224843A1 (en) | 2006-03-16 | 2007-09-20 | Pentabase Aps | Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids |
| US20080038783A1 (en) * | 2006-06-29 | 2008-02-14 | Applera Corporation | Compositions and Methods Pertaining to Guanylation of PNA Oligomers |
| EP2049688B1 (en) | 2006-08-01 | 2018-01-24 | Applied Biosystems, LLC | Detection of analytes and nucleic acids |
| CA2671194A1 (en) * | 2006-12-08 | 2008-06-19 | Asuragen, Inc. | Mir-20 regulated genes and pathways as targets for therapeutic intervention |
| US9938641B2 (en) * | 2006-12-18 | 2018-04-10 | Fluidigm Corporation | Selection of aptamers based on geometry |
| US20080274458A1 (en) * | 2007-05-01 | 2008-11-06 | Latham Gary J | Nucleic acid quantitation methods |
| US8361714B2 (en) | 2007-09-14 | 2013-01-29 | Asuragen, Inc. | Micrornas differentially expressed in cervical cancer and uses thereof |
| WO2009070805A2 (en) | 2007-12-01 | 2009-06-04 | Asuragen, Inc. | Mir-124 regulated genes and pathways as targets for therapeutic intervention |
| EP2990487A1 (en) | 2008-05-08 | 2016-03-02 | Asuragen, INC. | Compositions and methods related to mirna modulation of neovascularization or angiogenesis |
| US9534255B2 (en) * | 2008-12-17 | 2017-01-03 | Life Technologies Corporation | Methods, compositions, and kits for detecting allelic variants |
| WO2010111682A2 (en) | 2009-03-27 | 2010-09-30 | Life Technologies Corporation | Methods, compositions, and kits for detecting allelic variants |
| AU2010249421A1 (en) | 2009-05-22 | 2011-12-08 | Asuragen, Inc. | miRNA biomarkers of prostate disease |
| JP2012529908A (ja) | 2009-06-15 | 2012-11-29 | ネットバイオ・インコーポレーテッド | 法医学的ｄｎａの定量化のための改善された方法 |
| WO2011017567A1 (en) | 2009-08-05 | 2011-02-10 | Life Technologies Corporation | Methods for the analysis of proximity binding assay data |
| EP2775300A3 (en) | 2009-08-28 | 2015-04-01 | Asuragen, INC. | miRNA Biomarkers of Lung Disease |
| BR112012018394B8 (pt) * | 2009-12-21 | 2021-07-27 | Seegene Inc | método para detecção de uma sequência de ácido nucleico alvo e kit para detecção de uma sequência de ácido nucleico alvo |
| US9506057B2 (en) | 2010-03-26 | 2016-11-29 | Integrated Dna Technologies, Inc. | Modifications for antisense compounds |
| CA2794485C (en) | 2010-03-26 | 2018-05-22 | Integrated Dna Technologies, Inc. | Methods for enhancing nucleic acid hybridization |
| US20110276317A1 (en) | 2010-04-11 | 2011-11-10 | Life Technologies Corporation | SYSTEMS AND METHODS FOR MODEL-BASED qPCR |
| US20110287425A1 (en) * | 2010-05-14 | 2011-11-24 | Kromatid, Inc. | Assays to Detect Small-Scale Mutations in Individual Cells |
| CN106929506A (zh) | 2010-06-21 | 2017-07-07 | 生命技术公司 | 用于核酸的合成和/或检测的组合物、试剂盒及方法 |
| EP2582839B1 (en) | 2010-06-21 | 2016-05-18 | Life Technologies Corporation | Compositions, methods and kits for nucleic acid synthesis and amplification using rt |
| EP2896696B1 (en) | 2010-09-07 | 2017-12-27 | Integrated Dna Technologies, Inc. | Modifications for antisense compounds |
| DK2633071T3 (en) | 2010-10-27 | 2017-01-30 | Harvard College | COMPOSITIONS OF "MAINTENANCE" PRIMER DUPLEXES AND METHODS OF USE |
| EP2633080B1 (en) | 2010-10-29 | 2018-12-05 | President and Fellows of Harvard College | Method of detecting targets using fluorescently labelled nucleic acid nanotube probes |
| WO2012068276A2 (en) | 2010-11-16 | 2012-05-24 | Life Technologies Corporation | Systems and methods for the analysis of proximity binding assay data |
| EP2658998B1 (en) | 2010-12-29 | 2015-04-22 | Life Technologies Corporation | Ddao compounds as fluorescent reference standards |
| EP3567121B1 (en) | 2011-01-17 | 2023-08-30 | Life Technologies Corporation | Workflow for detection of ligands using nucleic acids |
| AU2012212105A1 (en) | 2011-02-03 | 2013-09-12 | Mirna Therapeutics, Inc. | Synthetic mimics of miR-124 |
| WO2012135620A2 (en) * | 2011-04-01 | 2012-10-04 | Advandx. Inc. | Molecular gram stain |
| US20140170653A1 (en) | 2011-04-15 | 2014-06-19 | Life Technologies Corporation | Chemical ligation |
| WO2012170907A2 (en) | 2011-06-08 | 2012-12-13 | Life Technologies Corporation | Polymerization of nucleic acids using proteins having low isoelectric points |
| WO2012170908A1 (en) | 2011-06-08 | 2012-12-13 | Life Technologies Corporation | Design and development of novel detergents for use in pcr systems |
| EP2723894B1 (en) | 2011-06-27 | 2016-05-11 | Eisai R&D Management Co., Ltd. | Microrna biomarkers indicative of alzheimer's disease |
| AU2012296385A1 (en) | 2011-08-18 | 2014-02-20 | Nestec S.A. | Compositions and methods for detecting allelic variants |
| US9644241B2 (en) | 2011-09-13 | 2017-05-09 | Interpace Diagnostics, Llc | Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease |
| US20130179086A1 (en) | 2011-11-29 | 2013-07-11 | Life Technologies Corporation | Systems and methods for the determination of a copy number of a genomic sequence |
| US9127317B2 (en) | 2012-03-02 | 2015-09-08 | Winthrop-University Hospital | Method for using probe based PCR detection to measure the levels of circulating demethylated β cell derived DNA as a measure of β cell loss in diabetes |
| JP6450308B2 (ja) | 2012-06-14 | 2019-01-09 | ライフ テクノロジーズ コーポレーション | ポリメラーゼ連鎖反応（ｐｃｒ）のための新規の組成物、方法、およびキット |
| WO2014059011A1 (en) | 2012-10-09 | 2014-04-17 | Advandx, Inc. | Devices, compositions and methods pertaining to microscopic analysis of microorganisms and other analytes of interest |
| ES3049590T3 (en) | 2012-11-02 | 2025-12-17 | Life Technologies Corp | Small rna capture, detection and quantification |
| WO2014093291A1 (en) | 2012-12-10 | 2014-06-19 | Advandx, Inc. | Use of probes for mass spectrometric identification of microorganisms or cells and associated conditions of interest |
| CN105339505B (zh) | 2013-03-12 | 2021-08-10 | 生命技术公司 | 基于通用报告体的基因分型方法和材料 |
| WO2014144121A2 (en) | 2013-03-15 | 2014-09-18 | Life Technologies Corporation | Classification and actionability indices for lung cancer |
| EP2981621B1 (en) | 2013-04-05 | 2019-05-22 | Life Technologies Corporation | Gene fusions |
| JP5928906B2 (ja) | 2013-08-27 | 2016-06-01 | 横河電機株式会社 | 核酸配列計測方法、核酸配列計測用デバイス、核酸配列計測用デバイスの製造方法および核酸配列計測装置 |
| EP3044324A1 (en) | 2013-09-13 | 2016-07-20 | Life Technologies Corporation | Classification and actionability indices for cancer |
| JP6742238B2 (ja) | 2013-10-25 | 2020-08-19 | ライフ テクノロジーズ コーポレーション | Ｐｃｒシステムにおいて使用するための新規化合物及びその用途 |
| ES2703792T3 (es) * | 2013-11-22 | 2019-03-12 | Orion Diagnostica Oy | Detección de ácidos nucleicos mediante amplificación basada en invasión de hebra |
| JP6706208B2 (ja) | 2014-01-05 | 2020-06-03 | バイオマーナ ホールディングス リミテッド | Ｍｉｒｎａ比率を使用する肺がん決定 |
| US10619210B2 (en) | 2014-02-07 | 2020-04-14 | The Johns Hopkins University | Predicting response to epigenetic drug therapy |
| EP3122901B1 (en) | 2014-03-27 | 2018-08-15 | Life Technologies Corporation | Gene fusions and gene variants associated with cancer |
| CN106414766B (zh) * | 2014-04-04 | 2019-10-08 | 帕纳金股份有限公司 | 使用pna探针的熔解曲线分析核苷酸多态性的方法和试剂盒 |
| CN107109474B (zh) | 2014-08-19 | 2021-03-19 | 豪夫迈·罗氏有限公司 | 用于核酸检测的方法和组合物 |
| WO2016094330A2 (en) | 2014-12-08 | 2016-06-16 | 20/20 Genesystems, Inc | Methods and machine learning systems for predicting the liklihood or risk of having cancer |
| WO2016092045A1 (en) | 2014-12-11 | 2016-06-16 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods and kits for predicting medically refractory acute severe colitis |
| ES2891085T3 (es) | 2015-01-30 | 2022-01-26 | Harvard College | Obtención de imágenes sin microscopio |
| WO2016177774A1 (en) | 2015-05-04 | 2016-11-10 | Academisch Medisch Centrum | Method of quantifying mirnas using normalization |
| CN108779486B (zh) | 2016-02-17 | 2023-02-28 | 哈佛学院院长及董事 | 分子编程工具 |
| RU2757416C2 (ru) | 2016-04-06 | 2021-10-15 | Лайф Текнолоджис Корпорейшн | Композиции, способы и наборы для синтеза и обнаружения нуклеиновых кислот |
| US20170362640A1 (en) | 2016-06-16 | 2017-12-21 | Life Technologies Corporation | Novel compositions, methods and kits for microorganism detection |
| CN109689886A (zh) | 2016-08-26 | 2019-04-26 | 生命技术公司 | 核酸提取和扩增对照及其使用方法 |
| WO2018045162A1 (en) | 2016-09-01 | 2018-03-08 | Biogen Ma Inc. | Biomarkers predictive of primary progressive multiple sclerosis and uses thereof |
| JP7116062B2 (ja) | 2017-01-10 | 2022-08-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 多重化シグナル増幅 |
| KR20200081476A (ko) | 2017-11-13 | 2020-07-07 | 라이프 테크놀로지스 코포레이션 | 요로 미생물 검출을 위한 조성물, 방법 및 키트 |
| CN111630179B (zh) | 2018-01-26 | 2024-07-19 | 哈佛学院院长及董事 | 邻近检测方法和组合物 |
| JP7308261B2 (ja) | 2018-08-30 | 2023-07-13 | ライフ テクノロジーズ コーポレーション | ジェノタイピングｐｃｒアッセイのための機械学習システム |
| US11836614B2 (en) | 2018-08-31 | 2023-12-05 | Life Technologies Corporation | Image driven quality control for array-based PCR |
| EP3719144A1 (en) | 2019-04-05 | 2020-10-07 | Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) | Mir-151a-3p as an universal endogenous control for exosome cargo normalization |
| US20220267857A1 (en) | 2019-07-19 | 2022-08-25 | Fundación Para La Investigación Biomédikca Del Hospital Universitario La Paz (Fibhulp) | Method for determining the response to treatment of a patient affected by non-small cell lung carcinoma (nsclc) |
| US20230304932A1 (en) | 2020-07-23 | 2023-09-28 | Life Technologies Corporation | Compositions, systems and methods for biological analysis involving energy transfer dye conjugates and analytes comprising the same |
| CA3186955A1 (en) | 2020-07-23 | 2022-01-27 | Scott Benson | Energy transfer dye conjugates for use in biological assays |
| US11649441B2 (en) | 2020-10-07 | 2023-05-16 | Abclonal Science, Inc. | Taq DNA polymerase mutants for probe qPCR |
| WO2022178216A2 (en) | 2021-02-22 | 2022-08-25 | Cotropia Joseph | Corona virus antigens and epitopes and proteins that bind thereto |
| WO2022197882A1 (en) | 2021-03-18 | 2022-09-22 | Cotropia Joseph | Human immunodeficiency virus (hiv) antigens and epitopes and proteins that bind thereto |
| WO2023004356A2 (en) | 2021-07-21 | 2023-01-26 | Life Technologies Corporation | Azaindole cyanine dyes, uses, and methods of preparation |
| CN117897454A (zh) | 2021-07-21 | 2024-04-16 | 生命技术公司 | 二苯并呫吨猝灭剂、用途和制备方法 |
| MX2024002584A (es) | 2021-08-30 | 2024-07-02 | Galderma Holding SA | Tratamientos para la dermatitis atopica. |
| WO2023039525A1 (en) | 2021-09-10 | 2023-03-16 | Life Technologies Corporation | Master mix compositions, kits, and methods |
| EP4426854A1 (en) | 2021-11-02 | 2024-09-11 | Life Technologies Corporation | Compositions and methods for reducing master mix contamination |
| WO2025007089A1 (en) | 2023-06-30 | 2025-01-02 | Life Technologies Corporation | Compositions, methods and kits for nucleic acids synthesis and amplification |
| WO2025151807A1 (en) | 2024-01-11 | 2025-07-17 | Life Technologies Corporation | Crude lysate sample extraction for digital pcr |
| WO2025175243A1 (en) | 2024-02-15 | 2025-08-21 | Life Technologies Corporation | Amplification and data collection protocol for rapid genotyping |
| WO2025175212A1 (en) | 2024-02-15 | 2025-08-21 | Life Technologies Corporation | Methods for rapid extraction-free genotyping |
| WO2025188713A1 (en) | 2024-03-05 | 2025-09-12 | Cotropia Joseph | Feline leukemia virus antigens and epitopes and proteins that bind thereto |
Family Cites Families (80)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4174384A (en) | 1975-06-30 | 1979-11-13 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4261968A (en) | 1979-05-10 | 1981-04-14 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US5288611A (en) | 1983-01-10 | 1994-02-22 | Gen-Probe Incorporated | Method for detecting, identifying, and quantitating organisms and viruses |
| US5688645A (en) | 1983-01-10 | 1997-11-18 | Gen-Probe Incorporated | Method for detecting, identifying, and quantitating non-viral organisms |
| US4542104A (en) | 1983-04-06 | 1985-09-17 | The Board Of Trustees Of The Leland Stanford Jr. Univ. | Phycobiliprotein fluorescent conjugates |
| US4766062A (en) | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay method and polynucleotide complex reagent therefor |
| US4666862A (en) | 1984-08-14 | 1987-05-19 | Ortho Diagnostic Systems Inc. | Fluorescent energy transfer with phycobiliproteins |
| FR2572180B1 (fr) * | 1984-10-24 | 1987-03-20 | Eric Marteau D Autry | Procede et appareil de reparation d'echantillons a des fins d'analyse |
| US4822733A (en) | 1985-05-28 | 1989-04-18 | Amoco Corporation | Lifetime-resolved assay procedures |
| US4725536A (en) | 1985-09-19 | 1988-02-16 | Genetics Institute, Inc. | Reagent polynucleotide complex with multiple target binding regions, and kit and methods |
| US4725537A (en) | 1985-09-19 | 1988-02-16 | Allied Corporation | Assay, reagent and kit employing nucleic acid strand displacement and restriction endonuclease cleavage |
| US4725538A (en) * | 1985-10-25 | 1988-02-16 | The Beth Israel Hospital Association | Method of assaying the presence of cancer cells |
| US4996143A (en) | 1985-12-23 | 1991-02-26 | Syngene, Inc. | Fluorescent stokes shift probes for polynucleotide hybridization |
| US4868103A (en) | 1986-02-19 | 1989-09-19 | Enzo Biochem, Inc. | Analyte detection by means of energy transfer |
| US5635347A (en) | 1986-04-30 | 1997-06-03 | Igen, Inc. | Rapid assays for amplification products |
| US5118801A (en) | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| JP3208486B2 (ja) | 1990-07-02 | 2001-09-10 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 蛍光エネルギー転移を利用する分析物の検出 |
| US5439793A (en) | 1990-07-19 | 1995-08-08 | Syntex (U.S.A.) Inc. | Method for producing a polynucleotide having an intramolecularly base-paired structure |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5237515A (en) | 1991-04-10 | 1993-08-17 | University Of Utah Research Foundation | Method of determining energy transfer efficiency of a donor-acceptor pair of fluorescent dyes |
| US5539082A (en) | 1993-04-26 | 1996-07-23 | Nielsen; Peter E. | Peptide nucleic acids |
| US5714331A (en) | 1991-05-24 | 1998-02-03 | Buchardt, Deceased; Ole | Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility |
| US5719262A (en) | 1993-11-22 | 1998-02-17 | Buchardt, Deceased; Ole | Peptide nucleic acids having amino acid side chains |
| CA2073630C (en) | 1991-08-30 | 2007-12-11 | Atsushi Ohshima | Method for synthesizing single-stranded stem-loop dnas, the products and uses therefor |
| US5787032A (en) | 1991-11-07 | 1998-07-28 | Nanogen | Deoxyribonucleic acid(DNA) optical storage using non-radiative energy transfer between a donor group, an acceptor group and a quencher group |
| CA2100159C (en) | 1991-11-15 | 2003-01-21 | John R. Link | Rapid assays for amplification products |
| US5348853A (en) | 1991-12-16 | 1994-09-20 | Biotronics Corporation | Method for reducing non-specific priming in DNA amplification |
| EP0552108B1 (en) | 1992-01-17 | 1999-11-10 | Lakowicz, Joseph R. | Energy transfer phase-modulation fluoro-immunoassay |
| WO1993016094A2 (en) | 1992-02-12 | 1993-08-19 | Chromagen, Inc. | Applications of fluorescent n-nucleosides and fluorescent structural analogs of n-nucleosides |
| DE69207580T2 (de) | 1992-03-23 | 1996-08-08 | Hoffmann La Roche | Verfahren zum DNS-Nachweis |
| US6020124A (en) | 1992-04-27 | 2000-02-01 | Trustees Of Dartmouth College | Detection of soluble gene sequences in biological fluids |
| GB9211979D0 (en) | 1992-06-05 | 1992-07-15 | Buchard Ole | Uses of nucleic acid analogues |
| EP0656068B1 (en) | 1992-07-24 | 1999-12-15 | University Of South Australia | Amplification and detection process |
| JPH06153997A (ja) | 1992-11-27 | 1994-06-03 | Canon Inc | 検出信号増幅による標的核酸の検出方法 |
| EP0601889A2 (en) | 1992-12-10 | 1994-06-15 | Maine Medical Center Research Institute | Nucleic acid probes |
| GB9311386D0 (en) | 1993-06-02 | 1993-07-21 | Pna Diagnostics As | Nucleic acid analogue assay procedures |
| DE69430824T2 (de) * | 1993-08-12 | 2003-01-23 | Neurotech S.A., Evry | Biokompatible immunoisolatorische Kapseln, die genetisch veränderte Zellen enthalten |
| US5527675A (en) | 1993-08-20 | 1996-06-18 | Millipore Corporation | Method for degradation and sequencing of polymers which sequentially eliminate terminal residues |
| CA2105364A1 (en) | 1993-09-01 | 1995-03-02 | Eric T. Kool | Stem-loop oligonucleotides containing parallel and antiparallel binding domains |
| DE4331012A1 (de) | 1993-09-13 | 1995-03-16 | Bayer Ag | Nukleinsäuren-bindende Oligomere mit N-Verzweigung für Therapie und Diagnostik |
| US6309823B1 (en) | 1993-10-26 | 2001-10-30 | Affymetrix, Inc. | Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same |
| US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| CA2176348C (en) | 1993-11-12 | 2004-11-02 | Sanjay Tyagi | Hybridization probes for nucleic acid detection, universal stems, methods and kits |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5654419A (en) | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
| US5539083A (en) | 1994-02-23 | 1996-07-23 | Isis Pharmaceuticals, Inc. | Peptide nucleic acid combinatorial libraries and improved methods of synthesis |
| WO1995032305A1 (en) | 1994-05-19 | 1995-11-30 | Dako A/S | Pna probes for detection of neisseria gonorrhoeae and chlamydia trachomatis |
| US5491063A (en) | 1994-09-01 | 1996-02-13 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
| US5629178A (en) | 1994-10-28 | 1997-05-13 | Genetics & Ivf Institute | Method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (PNA) |
| US5675517A (en) | 1995-04-25 | 1997-10-07 | Systemix | Fluorescence spectral overlap compensation for high speed flow cytometry systems |
| EP0826066B1 (en) | 1995-05-05 | 2000-09-13 | The Perkin-Elmer Corporation | Methods and reagents for combined pcr amplification and hybridization probing assay |
| US5879885A (en) | 1995-06-07 | 1999-03-09 | Gen-Probe Incorporated | Compositions for detecting and quantifying biological samples |
| WO1997007235A2 (en) | 1995-08-14 | 1997-02-27 | Abbott Laboratories | All-in-one nucleic acid amplification assay |
| US5770365A (en) | 1995-08-25 | 1998-06-23 | Tm Technologies, Inc. | Nucleic acid capture moieties |
| DE69637086T2 (de) | 1995-10-12 | 2008-01-10 | Lansdorp, Peter M., Vancouver | Verfahren zum nachweis von mehrfachkopien einer wiederholungssequenz in einem nukleinsäuremolekül |
| ES2289753T3 (es) | 1995-11-16 | 2008-02-01 | Dako Denmark A/S | Hibridacion in situ para detectar secuencias de acidos nucleicos especificas en muestras eucarioticas. |
| US5888733A (en) | 1995-11-16 | 1999-03-30 | Dako A/S | In situ hybridization to detect specific nucleic acid sequences in eucaryotic samples |
| EP0868534A4 (en) | 1995-12-18 | 2001-06-06 | Univ Washington | METHOD FOR NUCLEIC ACID ANALYSIS BY FLUORESCENT RESONANT ENERGY TRANSFER |
| US5723294A (en) | 1996-03-05 | 1998-03-03 | Gull Laboratories | Methods for detection and discrimination of multiple analytes using fluorescent technology |
| WO1998010096A1 (en) | 1996-04-12 | 1998-03-12 | The Public Health Research Institute Of The City Of New York, Inc. | Detection probes, kits and assays |
| JP3898228B2 (ja) * | 1996-04-12 | 2007-03-28 | ザ パブリック ヘルス リサーチ インスティチュート オブ ザ シティー オブ ニューヨーク インク | 検出プローブ、キット及びアッセイ |
| US5800996A (en) | 1996-05-03 | 1998-09-01 | The Perkin Elmer Corporation | Energy transfer dyes with enchanced fluorescence |
| SE506700C2 (sv) | 1996-05-31 | 1998-02-02 | Mikael Kubista | Sond och förfaranden för analys av nukleinsyra |
| DK1179600T3 (da) | 1996-06-04 | 2005-09-05 | Univ Utah Res Found | Overvågning af hybridisering under PCR |
| US5780233A (en) | 1996-06-06 | 1998-07-14 | Wisconsin Alumni Research Foundation | Artificial mismatch hybridization |
| US5866336A (en) | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US5827660A (en) | 1996-08-09 | 1998-10-27 | University Of Massachusetts | Caged fluorochrome-labeled probes and subtraction of autoflourescence background |
| US5691145A (en) | 1996-08-27 | 1997-11-25 | Becton, Dickinson And Company | Detection of nucleic acids using G-quartets |
| US5853992A (en) | 1996-10-04 | 1998-12-29 | The Regents Of The University Of California | Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels |
| WO1998018965A1 (en) | 1996-10-29 | 1998-05-07 | University Of Nebraska At Lincoln | Method for detecting point mutations in dna utilizing fluorescence energy transfer |
| US6110676A (en) | 1996-12-04 | 2000-08-29 | Boston Probes, Inc. | Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays |
| AU7847798A (en) | 1996-12-13 | 1998-07-03 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Fluorescent nucleotide analog hairpin formation for detection of nuclei c acid hybridization |
| US5837466A (en) | 1996-12-16 | 1998-11-17 | Vysis, Inc. | Devices and methods for detecting nucleic acid analytes in samples |
| US6025133A (en) | 1996-12-30 | 2000-02-15 | Gen-Probe Incorporated | Promoter-sequestered oligonucleoside and method of use |
| US5804386A (en) | 1997-01-15 | 1998-09-08 | Incyte Pharmaceuticals, Inc. | Sets of labeled energy transfer fluorescent primers and their use in multi component analysis |
| EP0968309B1 (en) | 1997-02-24 | 2004-10-13 | Georgia Tech Research Corporation | Method for determining a nucleic acid |
| US5846729A (en) | 1997-02-27 | 1998-12-08 | Lorne Park Research, Inc. | Assaying nucleotides in solution using a fluorescent intensity quenching effect |
| US6485901B1 (en) * | 1997-10-27 | 2002-11-26 | Boston Probes, Inc. | Methods, kits and compositions pertaining to linear beacons |
| AU1366299A (en) | 1997-10-27 | 1999-05-17 | Boston Probes, Inc. | Methods, kits and compositions pertaining to pna molecular beacons |
| US6361942B1 (en) | 1998-03-24 | 2002-03-26 | Boston Probes, Inc. | Method, kits and compositions pertaining to detection complexes |
-
1998
- 1998-10-26 US US09/179,162 patent/US6485901B1/en not_active Expired - Lifetime
- 1998-10-27 DE DE69842040T patent/DE69842040D1/de not_active Expired - Lifetime
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| WO1999021881A1 (en) | 1999-05-06 |
| EP1484337B1 (en) | 2010-12-08 |
| US20090136941A1 (en) | 2009-05-28 |
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| DE69842040D1 (de) | 2011-01-20 |
| EP1484337A3 (en) | 2005-06-29 |
| JP2009082137A (ja) | 2009-04-23 |
| AU1121499A (en) | 1999-05-17 |
| US6485901B1 (en) | 2002-11-26 |
| EP1484337A2 (en) | 2004-12-08 |
| ATE490978T1 (de) | 2010-12-15 |
| US20110250596A1 (en) | 2011-10-13 |
| DE69828432D1 (de) | 2005-02-03 |
| DE69828432T2 (de) | 2006-01-12 |
| EP1027372A1 (en) | 2000-08-16 |
| US20020064772A1 (en) | 2002-05-30 |
| EP1027372B1 (en) | 2004-12-29 |
| US7892741B2 (en) | 2011-02-22 |
| US20070178471A1 (en) | 2007-08-02 |
| ATE286066T1 (de) | 2005-01-15 |
| US7422852B2 (en) | 2008-09-09 |
| ES2235375T3 (es) | 2005-07-01 |
| US6649349B2 (en) | 2003-11-18 |
| DK1027372T3 (da) | 2005-05-09 |
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