JP3948682B2 - 殺虫性毒素 - Google Patents
殺虫性毒素 Download PDFInfo
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- JP3948682B2 JP3948682B2 JP53819497A JP53819497A JP3948682B2 JP 3948682 B2 JP3948682 B2 JP 3948682B2 JP 53819497 A JP53819497 A JP 53819497A JP 53819497 A JP53819497 A JP 53819497A JP 3948682 B2 JP3948682 B2 JP 3948682B2
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/60—Isolated nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2799/00—Uses of viruses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
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| US08/633,993 US6083499A (en) | 1996-04-19 | 1996-04-19 | Pesticidal toxins |
| US08/633,993 | 1996-04-19 | ||
| PCT/US1997/006463 WO1997040162A2 (en) | 1996-04-19 | 1997-04-18 | Pesticidal toxins |
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| JP2000509273A JP2000509273A (ja) | 2000-07-25 |
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| US6677148B1 (en) | 1996-04-19 | 2004-01-13 | Mycogen Corporation | Pesticidal proteins |
| WO1998040491A2 (en) * | 1997-03-13 | 1998-09-17 | Mycogen Corporation | Pesticidal bacillus thuringiensis strains |
| US6914131B1 (en) * | 1998-10-09 | 2005-07-05 | Chiron S.R.L. | Neisserial antigens |
| EP1710312B1 (en) | 1998-10-23 | 2009-06-03 | Mycogen Corporation | Plant-optimized polynucleotide encoding approximately 45 kDa pesticidal protein |
| DE69940962D1 (de) | 1998-10-23 | 2009-07-16 | Mycogen Corp | Für 45k Da Pestizidprotein kodierendes, pflanzen-optimiertes Polynukleotid |
| AU769939B2 (en) * | 1999-05-04 | 2004-02-12 | Monsanto Technology Llc | Coleopteran-toxic polypeptide compositions and insect-resistant transgenic plants |
| GB9911683D0 (en) * | 1999-05-19 | 1999-07-21 | Chiron Spa | Antigenic peptides |
| PT1790660E (pt) * | 2000-02-28 | 2012-09-17 | Novartis Vaccines & Diagnostic | Expressão heteróloga de proteínas de neisseria |
| US7091399B2 (en) | 2000-05-18 | 2006-08-15 | Bayer Bioscience N.V. | Transgenic plants expressing insecticidal proteins and methods of producing the same |
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| US9816104B2 (en) * | 2000-10-06 | 2017-11-14 | Monsanto Technology Llc | Compositions and methods for deploying a transgenic refuge as a seed blend |
| WO2004002223A2 (en) | 2002-06-28 | 2004-01-08 | Dow Agrosciences Llc | Pesticidally active proteins and polynucleotides obtainable from paenibacillus species |
| US7205454B2 (en) | 2002-07-31 | 2007-04-17 | Bayer Bioscience N.V. | Corn root preferential promoters and uses thereof |
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| EP3587573A1 (en) | 2011-06-16 | 2020-01-01 | The Regents of The University of California | Synthetic gene clusters |
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| US20170359965A1 (en) | 2014-12-19 | 2017-12-21 | E I Du Pont De Nemours And Company | Polylactic acid compositions with accelerated degradation rate and increased heat stability |
| CA3246568A1 (en) | 2015-01-15 | 2025-11-04 | Pioneer Hi Bred Int | Insecticidal proteins and methods for their use |
| EA038923B1 (ru) | 2015-03-11 | 2021-11-10 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидная днк-конструкция и способы её применения |
| CN116333064A (zh) | 2015-05-19 | 2023-06-27 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| CA2986265A1 (en) | 2015-06-16 | 2016-12-22 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| KR102197507B1 (ko) | 2015-07-13 | 2020-12-31 | 피벗 바이오, 인크. | 식물 형질 개선을 위한 방법 및 조성물 |
| UA124495C2 (uk) | 2015-08-06 | 2021-09-29 | Піонір Хай-Бред Інтернешнл, Інк. | Інсектицидний білок рослинного походження та спосіб його застосування |
| US11236347B2 (en) | 2015-08-28 | 2022-02-01 | Pioneer Hi-Bred International, Inc. | Ochrobactrum-mediated transformation of plants |
| AU2016336328A1 (en) | 2015-10-05 | 2018-04-19 | Massachusetts Institute Of Technology | Nitrogen fixation using refactored nif clusters |
| CA2998391C (en) | 2015-10-12 | 2023-09-12 | E. I. Du Pont De Nemours And Company | Fungal entomopathogen biocides and their use in plants |
| EP3390431A1 (en) | 2015-12-18 | 2018-10-24 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| EP4257694A3 (en) | 2015-12-22 | 2023-12-06 | Pioneer Hi-Bred International, Inc. | Tissue-preferred promoters and methods of use |
| US11781151B2 (en) | 2016-04-14 | 2023-10-10 | Pioneer Hi-Bred International, Inc. | Insecticidal CRY1B variants having improved activity spectrum and uses thereof |
| AR108284A1 (es) | 2016-04-19 | 2018-08-08 | Pioneer Hi Bred Int | Combinaciones insecticidas de polipéptidos que tienen espectro de actividad mejorado y usos de éstas |
| BR112018072417B1 (pt) | 2016-05-04 | 2023-03-14 | E. I. Du Pont De Nemours And Company | Polipeptídeo inseticida recombinante, polipeptídeo quimérico, composição, polinucleotídeo recombinante, construtos de dna, métodos para obter uma planta transgênica, métodos para inibir o crescimento ou extermínio de uma praga de inseto ou população de praga, método para obter uma célula procariótica transformada, célula procariótica transformada e método para modificar geneticamente o polipeptídeo inseticida |
| EP3472323A1 (en) | 2016-06-16 | 2019-04-24 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| CN116334123A (zh) | 2016-06-24 | 2023-06-27 | 先锋国际良种公司 | 植物调节元件及其使用方法 |
| CN109788735A (zh) | 2016-07-01 | 2019-05-21 | 先锋国际良种公司 | 来自植物的杀昆虫蛋白及其使用方法 |
| US20210292778A1 (en) | 2016-07-12 | 2021-09-23 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| CN110035662B (zh) | 2016-09-30 | 2022-09-30 | 美国陶氏益农公司 | 二元杀虫cry毒素 |
| EP3528617A4 (en) | 2016-10-21 | 2020-11-25 | Vestaron Corporation | CLIVABLE PEPTIDES AND PROTEINS, INSECTICIDES AND NEMATICIDES CONTAINING THEM |
| MX387927B (es) | 2016-11-01 | 2025-03-19 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para su uso. |
| CA3044404A1 (en) | 2016-12-14 | 2018-06-21 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| MX2019007491A (es) | 2016-12-22 | 2019-09-06 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para su uso. |
| CA3049258A1 (en) | 2017-01-12 | 2018-07-19 | Pivot Bio, Inc. | Methods and compositions for improving plant traits |
| WO2018140214A1 (en) | 2017-01-24 | 2018-08-02 | Pioneer Hi-Bred International, Inc. | Nematicidal protein from pseudomonas |
| US20190390219A1 (en) | 2017-02-08 | 2019-12-26 | Pioneer Hi-Bred International, Inc. | Insecticidal combinations of plant derived insecticidal proteins and methods for their use |
| EP3622076B1 (en) | 2017-05-11 | 2025-04-16 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| EP3630982A1 (en) | 2017-05-26 | 2020-04-08 | Pioneer Hi-Bred International, Inc. | Insecticidal polypeptides having improved activity spectrum and uses thereof |
| BR112020006045A2 (pt) | 2017-09-25 | 2020-10-06 | Pioneer Hi-Bred International, Inc. | molécula de ácido nucleico, cassete de expressão, vetor, célula vegetal, planta, semente da planta, método para produzir uma planta transgênica, planta transgênica, semente da planta transgênica, método para aprimorar a eficiência de maturação de embrião somático e método para produzir uma planta dicotiledônea transgênica ou uma planta gimnosperma transgênica |
| US20200165626A1 (en) | 2017-10-13 | 2020-05-28 | Pioneer Hi-Bred International, Inc. | Virus-induced gene silencing technology for insect control in maize |
| CA3079955A1 (en) | 2017-10-25 | 2019-05-02 | Pivot Bio, Inc. | Methods and compositions for improving engineered microbes that fix nitrogen |
| CN117757706A (zh) | 2017-10-25 | 2024-03-26 | 皮沃特生物股份有限公司 | 靶向改良植物性状的固氮的基因靶标 |
| PL3740070T3 (pl) | 2018-01-18 | 2025-03-03 | Pioneer Hi-Bred International Inc. | Enkapsulacja alginianowa mikrosklerocjów grzybów |
| KR20200123144A (ko) | 2018-02-22 | 2020-10-28 | 지머젠 인코포레이티드 | 바실러스가 농축된 게놈 라이브러리를 생성하고 새로운 cry 독소를 동정하기 위한 방법 |
| AR114446A1 (es) | 2018-03-02 | 2020-09-09 | Zymergen Inc | Plataforma de descubrimiento de proteínas insecticidas, y proteínas insecticidas descubiertas a partir de la misma |
| US20210002657A1 (en) | 2018-03-02 | 2021-01-07 | Pioneer Hi-Bred International, Inc. | Plant health assay |
| CN111886337B (zh) | 2018-03-12 | 2025-01-07 | 先锋国际良种公司 | 形态发生因子用于改善基因编辑的用途 |
| MX2020009435A (es) | 2018-03-14 | 2020-11-11 | Pioneer Hi Bred Int | Proteinas con actividad insecticida de plantas y metodos para sus usos. |
| CN111867377B (zh) | 2018-03-14 | 2023-05-23 | 先锋国际良种公司 | 来自植物的杀昆虫蛋白及其使用方法 |
| US20240247279A1 (en) | 2018-04-27 | 2024-07-25 | Pioneer Hi-Bred International, Inc. | Maize event dp-023211-2 and methods for detection thereof |
| US11859197B2 (en) | 2018-05-03 | 2024-01-02 | Evogene Ltd. | Insecticidal polypeptides and use thereof |
| BR112020023800A2 (pt) | 2018-05-22 | 2021-02-23 | Pioneer Hi-Bred International, Inc. | elementos reguladores de planta e métodos de uso dos mesmos |
| BR112020026771A2 (pt) | 2018-06-27 | 2021-03-30 | Pivot Bio, Inc. | Composições agrícolas que compreendem micróbios de fixação de nitrogênio remodelados |
| WO2020005933A1 (en) | 2018-06-28 | 2020-01-02 | Pioneer Hi-Bred International, Inc. | Methods for selecting transformed plants |
| WO2020014498A1 (en) | 2018-07-11 | 2020-01-16 | Pivot Bio, Inc. | Temporally and spatially targeted dynamic nitrogen delivery by remodeled microbes |
| CN119060146A (zh) | 2018-08-29 | 2024-12-03 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| KR20210084482A (ko) | 2018-10-31 | 2021-07-07 | 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 | 오크로박테륨 매개된 식물 형질전환을 위한 조성물 및 방법 |
| EP3893647A1 (en) | 2018-12-14 | 2021-10-20 | Pioneer Hi-Bred International, Inc. | Biologicals and their use in plants |
| US12281299B2 (en) | 2019-03-19 | 2025-04-22 | Massachusetts Institute Of Technology | Control of nitrogen fixation in rhizobia that associate with cereals |
| WO2021076346A1 (en) | 2019-10-18 | 2021-04-22 | Pioneer Hi-Bred International, Inc. | Maize event dp-202216-6 and dp-023211-2 stack |
| ES2973551T3 (es) | 2020-02-04 | 2024-06-20 | Corteva Agriscience Llc | Composiciones con utilidad como plaguicidas y procedimientos relacionados con ellas |
| CA3172637A1 (en) | 2020-05-01 | 2021-11-04 | Pivot Bio, Inc. | Measurement of nitrogen fixation and incorporation |
| EP4182466A2 (en) | 2020-07-14 | 2023-05-24 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| EP4192848A1 (en) | 2020-08-10 | 2023-06-14 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
| TW202345696A (zh) | 2022-05-18 | 2023-12-01 | 美商科迪華農業科技有限責任公司 | 具有殺有害生物效用之組成物及與其相關的方法 |
| WO2024020360A1 (en) | 2022-07-18 | 2024-01-25 | Pairwise Plants Services, Inc. | Mustard green plants named 'pwrg-1', 'pwrg-2,' and 'pwsgc' |
| WO2025178772A1 (en) | 2024-02-23 | 2025-08-28 | Genective Sa | Insecticidal proteins compositions and methods of use |
| WO2025193453A1 (en) | 2024-03-14 | 2025-09-18 | Genective Sa | Insecticidal proteins compositions and methods of use |
| WO2025235220A1 (en) | 2024-05-08 | 2025-11-13 | Genective Sa | Insecticidal proteins compositions and methods of use |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4448885A (en) * | 1981-04-27 | 1984-05-15 | Board Of The Regents Of The University Of Washington | Bacillus thuringiensis crystal protein in Escherichia coli |
| US4467036A (en) * | 1981-11-12 | 1984-08-21 | The Board Of Regents Of The University Of Washington | Bacillus thuringiensis crystal protein in Escherichia coli |
| US4797276A (en) * | 1985-03-22 | 1989-01-10 | Mycogen Corporation | Control of cotton boll weevil, alfalfa weevil, and corn rootworm via contact with a strain of Bacillus thuringiensis |
| US4918006A (en) * | 1985-07-01 | 1990-04-17 | E. I. Du Pont De Nemours And Company | Gene coding for insecticidal crystal protein |
| US4853331A (en) * | 1985-08-16 | 1989-08-01 | Mycogen Corporation | Cloning and expression of Bacillus thuringiensis toxin gene toxic to beetles of the order Coleoptera |
| US4948734A (en) * | 1987-08-12 | 1990-08-14 | Mycogen Corporation | Novel isolates of bacillus thuringiensis having activity against nematodes |
| US5151363A (en) * | 1990-07-27 | 1992-09-29 | Mycogen Corporation | Isolates of Bacillus thuringiensis that are active against nematodes |
| US4849217A (en) * | 1987-11-19 | 1989-07-18 | Mycogen Corporation | Novel isolates of bacilus thuringiensis having activity against the alfalfa weevil, hypera brunneipennis |
| NZ230375A (en) * | 1988-09-09 | 1991-07-26 | Lubrizol Genetics Inc | Synthetic gene encoding b. thuringiensis insecticidal protein |
| GB9009571D0 (en) * | 1990-04-27 | 1990-06-20 | Univ Singapore | Expression of insecticidal proteins |
| CA2042868A1 (en) * | 1990-06-11 | 1991-12-12 | Kenneth E. Narva | Bacillus thuringiensis microbes active against nematodes, and genes encoding novel nematode-active toxins cloned from bacillus thuringiensis isolates |
| US5204100A (en) * | 1991-02-01 | 1993-04-20 | Ciba-Geigy Corporation | Baciullus thuringiensis strains active against coleopteran insects |
| US5208017A (en) * | 1991-02-21 | 1993-05-04 | Mycogen Corporation | Biologically active Bacillus thuringiensis isolates |
| AU5987794A (en) * | 1992-12-31 | 1994-08-15 | Mycogen Corporation | Novel (bacillus thuringiensis) toxins active against corn rootworm larvae |
| AU686213B2 (en) * | 1993-07-15 | 1998-02-05 | Mycogen Corporation | Materials and methods for the control of calliphoridae pests |
| US6083499A (en) * | 1996-04-19 | 2000-07-04 | Mycogen Corporation | Pesticidal toxins |
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| ATE395417T1 (de) | 2008-05-15 |
| JP2000509273A (ja) | 2000-07-25 |
| CA2251560C (en) | 2010-06-29 |
| CA2251560A1 (en) | 1997-10-30 |
| US6548291B1 (en) | 2003-04-15 |
| ES2306458T3 (es) | 2008-11-01 |
| DE69738689D1 (de) | 2008-06-26 |
| US20030167522A1 (en) | 2003-09-04 |
| US6624145B1 (en) | 2003-09-23 |
| US6083499A (en) | 2000-07-04 |
| WO1997040162A2 (en) | 1997-10-30 |
| US6893872B2 (en) | 2005-05-17 |
| KR20000010538A (ko) | 2000-02-15 |
| EP0914439A2 (en) | 1999-05-12 |
| EP0914439B1 (en) | 2008-05-14 |
| WO1997040162A3 (en) | 1998-03-26 |
| US6127180A (en) | 2000-10-03 |
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