JP3594598B2 - 改良された核酸定量方法 - Google Patents
改良された核酸定量方法 Download PDFInfo
- Publication number
- JP3594598B2 JP3594598B2 JP50383695A JP50383695A JP3594598B2 JP 3594598 B2 JP3594598 B2 JP 3594598B2 JP 50383695 A JP50383695 A JP 50383695A JP 50383695 A JP50383695 A JP 50383695A JP 3594598 B2 JP3594598 B2 JP 3594598B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- sample
- amount
- analyte
- construct
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- AIDS & HIV (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP93202015 | 1993-07-09 | ||
EP93202015.9 | 1993-07-09 | ||
EP93203424.2 | 1993-12-06 | ||
EP93203424 | 1993-12-06 | ||
PCT/EP1994/002295 WO1995002067A1 (en) | 1993-07-09 | 1994-07-08 | Improved method for the quantification of nucleic acid |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH08501222A JPH08501222A (ja) | 1996-02-13 |
JP3594598B2 true JP3594598B2 (ja) | 2004-12-02 |
Family
ID=26133903
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP50383695A Expired - Lifetime JP3594598B2 (ja) | 1993-07-09 | 1994-07-08 | 改良された核酸定量方法 |
Country Status (10)
Country | Link |
---|---|
JP (1) | JP3594598B2 (fi) |
KR (1) | KR100312800B1 (fi) |
AT (1) | ATE178946T1 (fi) |
AU (1) | AU668659B2 (fi) |
CA (1) | CA2143857C (fi) |
DE (1) | DE69417863T2 (fi) |
DK (1) | DK0662156T3 (fi) |
ES (1) | ES2131699T3 (fi) |
FI (1) | FI112094B (fi) |
WO (1) | WO1995002067A1 (fi) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9388467B2 (en) | 2009-09-16 | 2016-07-12 | Seiko Epson Corporation | Biochip and target DNA quantitative method |
US9580761B2 (en) | 2011-02-25 | 2017-02-28 | Novartis Ag | Exogenous internal positive control |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH09505464A (ja) * | 1993-09-22 | 1997-06-03 | イゲン,インコーポレーテッド | 自立性配列複製電気化学発光核酸測定法 |
CA2159044A1 (en) * | 1994-09-26 | 1996-03-27 | Falko-Guenter Falkner | Method of quantitating nucleic acids |
US5705365A (en) * | 1995-06-07 | 1998-01-06 | Gen-Probe Incorporated | Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
WO1996040990A1 (en) * | 1995-06-07 | 1996-12-19 | Gen-Probe Incorporated | Methods and kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
US5710029A (en) * | 1995-06-07 | 1998-01-20 | Gen-Probe Incorporated | Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
GB9519638D0 (en) * | 1995-09-26 | 1995-11-29 | Dynal As | Method |
US5795722A (en) * | 1997-03-18 | 1998-08-18 | Visible Genetics Inc. | Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample |
US5677124A (en) * | 1996-07-03 | 1997-10-14 | Ambion, Inc. | Ribonuclease resistant viral RNA standards |
US5939262A (en) * | 1996-07-03 | 1999-08-17 | Ambion, Inc. | Ribonuclease resistant RNA preparation and utilization |
US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
FR2765590B1 (fr) * | 1997-07-01 | 2002-08-09 | Pasteur Institut | Methode de semi-quantification d'alleles |
WO2000000638A2 (en) * | 1998-06-26 | 2000-01-06 | Akzo Nobel N.V. | Tagging of rna amplicons generated by transcription-based amplification |
EP1013776A1 (en) * | 1998-12-22 | 2000-06-28 | Universiteit van Amsterdam | A sensitive assay for the detection or quantitation of human cytomegalovirus nucleic acid |
GB9902422D0 (en) * | 1999-02-03 | 1999-03-24 | Lgc Teddington Limited | Reference material for nucleic acid amplification |
CA2378408C (en) * | 1999-07-23 | 2010-07-06 | Gen-Probe Incorporated | Polynucleotide amplification method |
AT409383B (de) * | 1999-12-22 | 2002-07-25 | Baxter Ag | Verfahren zur detektion und quantifizierung von nukleinsäuren in einer probe |
JP3752488B2 (ja) | 2000-09-22 | 2006-03-08 | 株式会社日立製作所 | 核酸解析方法 |
US6312929B1 (en) * | 2000-12-22 | 2001-11-06 | Cepheid | Compositions and methods enabling a totally internally controlled amplification reaction |
JP5564795B2 (ja) * | 2009-01-06 | 2014-08-06 | 株式会社島津製作所 | Dna定量方法、及び遺伝子解析方法 |
KR102108855B1 (ko) | 2017-10-30 | 2020-05-12 | 한국표준과학연구원 | 안정동위원소 표지 핵산을 내부표준물질로 활용하는 핵산 정량 방법 및 이의 용도 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4942124A (en) * | 1987-08-11 | 1990-07-17 | President And Fellows Of Harvard College | Multiplex sequencing |
EP0525882B1 (en) * | 1991-08-02 | 2004-01-14 | bioMerieux B.V. | Quantification of nucleic acid |
FR2683827B1 (fr) * | 1991-11-15 | 1994-03-04 | Institut Nal Sante Recherc Medic | Procede de determination de la quantite d'un fragment d'adn d'interet par une methode d'amplification enzymatique. |
-
1994
- 1994-07-08 DK DK94924750T patent/DK0662156T3/da active
- 1994-07-08 ES ES94924750T patent/ES2131699T3/es not_active Expired - Lifetime
- 1994-07-08 AU AU74928/94A patent/AU668659B2/en not_active Expired
- 1994-07-08 CA CA002143857A patent/CA2143857C/en not_active Expired - Lifetime
- 1994-07-08 DE DE69417863T patent/DE69417863T2/de not_active Expired - Lifetime
- 1994-07-08 KR KR1019950700919A patent/KR100312800B1/ko not_active IP Right Cessation
- 1994-07-08 JP JP50383695A patent/JP3594598B2/ja not_active Expired - Lifetime
- 1994-07-08 WO PCT/EP1994/002295 patent/WO1995002067A1/en active IP Right Grant
- 1994-07-08 AT AT94924750T patent/ATE178946T1/de active
-
1995
- 1995-03-08 FI FI951082A patent/FI112094B/fi not_active IP Right Cessation
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9388467B2 (en) | 2009-09-16 | 2016-07-12 | Seiko Epson Corporation | Biochip and target DNA quantitative method |
US9416418B2 (en) | 2009-09-16 | 2016-08-16 | Seiko Epson Corporation | Biochip and target DNA quantitative method |
US9580761B2 (en) | 2011-02-25 | 2017-02-28 | Novartis Ag | Exogenous internal positive control |
Also Published As
Publication number | Publication date |
---|---|
KR100312800B1 (ko) | 2002-02-28 |
AU668659B2 (en) | 1996-05-09 |
DE69417863T2 (de) | 1999-10-07 |
CA2143857C (en) | 2005-09-20 |
JPH08501222A (ja) | 1996-02-13 |
FI951082A (fi) | 1995-03-08 |
FI951082A0 (fi) | 1995-03-08 |
CA2143857A1 (en) | 1995-01-19 |
FI112094B (fi) | 2003-10-31 |
AU7492894A (en) | 1995-02-06 |
DE69417863D1 (de) | 1999-05-20 |
ATE178946T1 (de) | 1999-04-15 |
ES2131699T3 (es) | 1999-08-01 |
DK0662156T3 (da) | 1999-10-25 |
WO1995002067A1 (en) | 1995-01-19 |
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