JP3594598B2 - 改良された核酸定量方法 - Google Patents

改良された核酸定量方法 Download PDF

Info

Publication number
JP3594598B2
JP3594598B2 JP50383695A JP50383695A JP3594598B2 JP 3594598 B2 JP3594598 B2 JP 3594598B2 JP 50383695 A JP50383695 A JP 50383695A JP 50383695 A JP50383695 A JP 50383695A JP 3594598 B2 JP3594598 B2 JP 3594598B2
Authority
JP
Japan
Prior art keywords
nucleic acid
sample
amount
analyte
construct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP50383695A
Other languages
English (en)
Japanese (ja)
Other versions
JPH08501222A (ja
Inventor
ベウニンゲン,マリナス・ヘラルドス・ヨハネス
キービツツ,テイム
ベウメル,トーマス・アウグステイヌス・マリア
Original Assignee
アクゾ・ノベル・エヌ・ベー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by アクゾ・ノベル・エヌ・ベー filed Critical アクゾ・ノベル・エヌ・ベー
Publication of JPH08501222A publication Critical patent/JPH08501222A/ja
Application granted granted Critical
Publication of JP3594598B2 publication Critical patent/JP3594598B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6865Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/702Specific hybridization probes for retroviruses
    • C12Q1/703Viruses associated with AIDS

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP50383695A 1993-07-09 1994-07-08 改良された核酸定量方法 Expired - Lifetime JP3594598B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP93202015 1993-07-09
EP93202015.9 1993-07-09
EP93203424.2 1993-12-06
EP93203424 1993-12-06
PCT/EP1994/002295 WO1995002067A1 (en) 1993-07-09 1994-07-08 Improved method for the quantification of nucleic acid

Publications (2)

Publication Number Publication Date
JPH08501222A JPH08501222A (ja) 1996-02-13
JP3594598B2 true JP3594598B2 (ja) 2004-12-02

Family

ID=26133903

Family Applications (1)

Application Number Title Priority Date Filing Date
JP50383695A Expired - Lifetime JP3594598B2 (ja) 1993-07-09 1994-07-08 改良された核酸定量方法

Country Status (10)

Country Link
JP (1) JP3594598B2 (fi)
KR (1) KR100312800B1 (fi)
AT (1) ATE178946T1 (fi)
AU (1) AU668659B2 (fi)
CA (1) CA2143857C (fi)
DE (1) DE69417863T2 (fi)
DK (1) DK0662156T3 (fi)
ES (1) ES2131699T3 (fi)
FI (1) FI112094B (fi)
WO (1) WO1995002067A1 (fi)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9388467B2 (en) 2009-09-16 2016-07-12 Seiko Epson Corporation Biochip and target DNA quantitative method
US9580761B2 (en) 2011-02-25 2017-02-28 Novartis Ag Exogenous internal positive control

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09505464A (ja) * 1993-09-22 1997-06-03 イゲン,インコーポレーテッド 自立性配列複製電気化学発光核酸測定法
CA2159044A1 (en) * 1994-09-26 1996-03-27 Falko-Guenter Falkner Method of quantitating nucleic acids
US5705365A (en) * 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
WO1996040990A1 (en) * 1995-06-07 1996-12-19 Gen-Probe Incorporated Methods and kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
GB9519638D0 (en) * 1995-09-26 1995-11-29 Dynal As Method
US5795722A (en) * 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
US5677124A (en) * 1996-07-03 1997-10-14 Ambion, Inc. Ribonuclease resistant viral RNA standards
US5939262A (en) * 1996-07-03 1999-08-17 Ambion, Inc. Ribonuclease resistant RNA preparation and utilization
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
FR2765590B1 (fr) * 1997-07-01 2002-08-09 Pasteur Institut Methode de semi-quantification d'alleles
WO2000000638A2 (en) * 1998-06-26 2000-01-06 Akzo Nobel N.V. Tagging of rna amplicons generated by transcription-based amplification
EP1013776A1 (en) * 1998-12-22 2000-06-28 Universiteit van Amsterdam A sensitive assay for the detection or quantitation of human cytomegalovirus nucleic acid
GB9902422D0 (en) * 1999-02-03 1999-03-24 Lgc Teddington Limited Reference material for nucleic acid amplification
CA2378408C (en) * 1999-07-23 2010-07-06 Gen-Probe Incorporated Polynucleotide amplification method
AT409383B (de) * 1999-12-22 2002-07-25 Baxter Ag Verfahren zur detektion und quantifizierung von nukleinsäuren in einer probe
JP3752488B2 (ja) 2000-09-22 2006-03-08 株式会社日立製作所 核酸解析方法
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
JP5564795B2 (ja) * 2009-01-06 2014-08-06 株式会社島津製作所 Dna定量方法、及び遺伝子解析方法
KR102108855B1 (ko) 2017-10-30 2020-05-12 한국표준과학연구원 안정동위원소 표지 핵산을 내부표준물질로 활용하는 핵산 정량 방법 및 이의 용도

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
EP0525882B1 (en) * 1991-08-02 2004-01-14 bioMerieux B.V. Quantification of nucleic acid
FR2683827B1 (fr) * 1991-11-15 1994-03-04 Institut Nal Sante Recherc Medic Procede de determination de la quantite d'un fragment d'adn d'interet par une methode d'amplification enzymatique.

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9388467B2 (en) 2009-09-16 2016-07-12 Seiko Epson Corporation Biochip and target DNA quantitative method
US9416418B2 (en) 2009-09-16 2016-08-16 Seiko Epson Corporation Biochip and target DNA quantitative method
US9580761B2 (en) 2011-02-25 2017-02-28 Novartis Ag Exogenous internal positive control

Also Published As

Publication number Publication date
KR100312800B1 (ko) 2002-02-28
AU668659B2 (en) 1996-05-09
DE69417863T2 (de) 1999-10-07
CA2143857C (en) 2005-09-20
JPH08501222A (ja) 1996-02-13
FI951082A (fi) 1995-03-08
FI951082A0 (fi) 1995-03-08
CA2143857A1 (en) 1995-01-19
FI112094B (fi) 2003-10-31
AU7492894A (en) 1995-02-06
DE69417863D1 (de) 1999-05-20
ATE178946T1 (de) 1999-04-15
ES2131699T3 (es) 1999-08-01
DK0662156T3 (da) 1999-10-25
WO1995002067A1 (en) 1995-01-19

Similar Documents

Publication Publication Date Title
US5837501A (en) Nucleic acid quantitation by co-amplification of target with multiple internal controls
JP3594598B2 (ja) 改良された核酸定量方法
US5834255A (en) Quantification of nucleic acid
US7384769B2 (en) Method for the quantitative detection of nucleic acids
US5800984A (en) Nucleic acid sequence detection by triple helix formation at primer site in amplification reactions
US6541205B1 (en) Method for assaying nucleic acid
KR100615407B1 (ko) Hiv-1의 모든 아형의 증폭 및 검출시 프라이머 및프로브로서 사용할 수 있는 핵산 서열
JP3560974B2 (ja) 核酸検出における偽陰性の除外
IE83814B1 (en) Quantification of nucleic acid
US20030157499A1 (en) Method of assessing the amount of nucleic acid in a sample
EP3018218B1 (en) Methods for detection of nucleic acid sequences over an extended dynamic range
Romano et al. NASBA: a novel, isothermal detection technology for qualitative and quantitative HIV-1 RNA measurements
JP2009000063A (ja) 改良されたノロウイルスrna検出方法
JP4209977B2 (ja) 淋菌の核酸の増幅および検出による淋菌の検出
US6830887B2 (en) Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
US20060228698A1 (en) Oligonucleotide for detection of HIV-1 and detection method
EP0662156B1 (en) Improved method for the quantification of nucleic acid
JP2009017824A (ja) 改良されたノロウイルスrnaの検出方法
US6881544B2 (en) Method of amplifying or detecting HIV-1 RNA
US6756198B2 (en) Oligonucleotide for highly sensitive detection of hepatitis C virus and method for detection thereof
JPWO2006011667A1 (ja) ヘテロ核リボヌクレオチドタンパク質B1(hnRNPB1)mRNAの測定方法
JPH11103897A (ja) リアルタイム検出pcr法によるhbv遺伝子の測定方法並びにそれに用いられるプライマー及びプローブ
JP2000139500A (ja) リアルタイム検出pcrによるhiv遺伝子の測定方法並びにそれに用いられるプライマー及びプローブ
Romano et al. Quantitation of RNA by NASBA™: Applications and Issues for HIV-1 and AIDS
JP2001340086A (ja) 腸炎ビブリオ菌の耐熱性溶血毒遺伝子の検出法

Legal Events

Date Code Title Description
A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20040406

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20040706

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20040824

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20040901

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20080910

Year of fee payment: 4

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20080910

Year of fee payment: 4

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20090910

Year of fee payment: 5

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20100910

Year of fee payment: 6

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20100910

Year of fee payment: 6

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110910

Year of fee payment: 7

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120910

Year of fee payment: 8

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130910

Year of fee payment: 9

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

EXPY Cancellation because of completion of term