CA2143857C - Improved method for the quantification of nucleic acid - Google Patents

Improved method for the quantification of nucleic acid Download PDF

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Publication number
CA2143857C
CA2143857C CA002143857A CA2143857A CA2143857C CA 2143857 C CA2143857 C CA 2143857C CA 002143857 A CA002143857 A CA 002143857A CA 2143857 A CA2143857 A CA 2143857A CA 2143857 C CA2143857 C CA 2143857C
Authority
CA
Canada
Prior art keywords
nucleic acid
analyte
sample
constructs
amount
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002143857A
Other languages
English (en)
French (fr)
Other versions
CA2143857A1 (en
Inventor
Marinus G. J. Van Beuningen
Tim Kievits
Thomas A. M. Beumer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux BV
Original Assignee
Biomerieux BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux BV filed Critical Biomerieux BV
Publication of CA2143857A1 publication Critical patent/CA2143857A1/en
Application granted granted Critical
Publication of CA2143857C publication Critical patent/CA2143857C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002143857A 1993-07-09 1994-07-08 Improved method for the quantification of nucleic acid Expired - Lifetime CA2143857C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP93202015.9 1993-07-09
EP93202015 1993-07-09
EP93203424.2 1993-12-06
EP93203424 1993-12-06
PCT/EP1994/002295 WO1995002067A1 (en) 1993-07-09 1994-07-08 Improved method for the quantification of nucleic acid

Publications (2)

Publication Number Publication Date
CA2143857A1 CA2143857A1 (en) 1995-01-19
CA2143857C true CA2143857C (en) 2005-09-20

Family

ID=26133903

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002143857A Expired - Lifetime CA2143857C (en) 1993-07-09 1994-07-08 Improved method for the quantification of nucleic acid

Country Status (10)

Country Link
JP (1) JP3594598B2 (fi)
KR (1) KR100312800B1 (fi)
AT (1) ATE178946T1 (fi)
AU (1) AU668659B2 (fi)
CA (1) CA2143857C (fi)
DE (1) DE69417863T2 (fi)
DK (1) DK0662156T3 (fi)
ES (1) ES2131699T3 (fi)
FI (1) FI112094B (fi)
WO (1) WO1995002067A1 (fi)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2172248C (en) * 1993-09-22 2003-12-30 John Kenten Self-sustained sequence replication electrochemiluminescent nucleic acid assay
CA2159044A1 (en) * 1994-09-26 1996-03-27 Falko-Guenter Falkner Method of quantitating nucleic acids
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5705365A (en) * 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
JP3996639B2 (ja) * 1995-06-07 2007-10-24 ジェン−プローブ・インコーポレーテッド 生成物の増幅後レベルから核酸標的配列の増幅前レベルを決定するための方法とキット
GB9519638D0 (en) * 1995-09-26 1995-11-29 Dynal As Method
US5795722A (en) * 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
US5677124A (en) * 1996-07-03 1997-10-14 Ambion, Inc. Ribonuclease resistant viral RNA standards
US5939262A (en) 1996-07-03 1999-08-17 Ambion, Inc. Ribonuclease resistant RNA preparation and utilization
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
FR2765590B1 (fr) * 1997-07-01 2002-08-09 Pasteur Institut Methode de semi-quantification d'alleles
WO2000000638A2 (en) * 1998-06-26 2000-01-06 Akzo Nobel N.V. Tagging of rna amplicons generated by transcription-based amplification
EP1013776A1 (en) * 1998-12-22 2000-06-28 Universiteit van Amsterdam A sensitive assay for the detection or quantitation of human cytomegalovirus nucleic acid
GB9902422D0 (en) * 1999-02-03 1999-03-24 Lgc Teddington Limited Reference material for nucleic acid amplification
EP1238100B1 (en) * 1999-07-23 2005-09-07 Gen-Probe Incorporated Polynucleotide amplification method
AT409383B (de) * 1999-12-22 2002-07-25 Baxter Ag Verfahren zur detektion und quantifizierung von nukleinsäuren in einer probe
JP3752488B2 (ja) * 2000-09-22 2006-03-08 株式会社日立製作所 核酸解析方法
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
JP5564795B2 (ja) * 2009-01-06 2014-08-06 株式会社島津製作所 Ｄｎａ定量方法、及び遺伝子解析方法
JP2011062119A (ja) 2009-09-16 2011-03-31 Seiko Epson Corp 生体試料定量用チップ
CA2828192A1 (en) * 2011-02-25 2012-08-30 Novartis Ag Exogenous internal positive control
KR102108855B1 (ko) 2017-10-30 2020-05-12 한국표준과학연구원 안정동위원소 표지 핵산을 내부표준물질로 활용하는 핵산 정량 방법 및 이의 용도

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
ATE257860T1 (de) * 1991-08-02 2004-01-15 Biomerieux Bv Quantifikation von nukleinsäuren
FR2683827B1 (fr) * 1991-11-15 1994-03-04 Institut Nal Sante Recherc Medic Procede de determination de la quantite d'un fragment d'adn d'interet par une methode d'amplification enzymatique.

Also Published As

Publication number Publication date
DE69417863D1 (de) 1999-05-20
KR100312800B1 (ko) 2002-02-28
DE69417863T2 (de) 1999-10-07
DK0662156T3 (da) 1999-10-25
FI951082A (fi) 1995-03-08
AU7492894A (en) 1995-02-06
CA2143857A1 (en) 1995-01-19
AU668659B2 (en) 1996-05-09
ES2131699T3 (es) 1999-08-01
WO1995002067A1 (en) 1995-01-19
JP3594598B2 (ja) 2004-12-02
FI112094B (fi) 2003-10-31
JPH08501222A (ja) 1996-02-13
FI951082A0 (fi) 1995-03-08
ATE178946T1 (de) 1999-04-15

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Effective date: 20140708