JP2021507694A5 - - Google Patents

Info

Publication number

JP2021507694A5

JP2021507694A5 JP2020532631A JP2020532631A JP2021507694A5 JP 2021507694 A5 JP2021507694 A5 JP 2021507694A5 JP 2020532631 A JP2020532631 A JP 2020532631A JP 2020532631 A JP2020532631 A JP 2020532631A JP 2021507694 A5 JP2021507694 A5 JP 2021507694A5

Authority

JP

Japan

Prior art keywords

dna polymerase

seq

sequence

amino acid

aspartic acid

Prior art date

2018-12-17

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

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• 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 38
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 38
• CKLJMWTZIZZHCS-REOHCLBHSA-N aspartic acid group Chemical group N[C@@H](CC(=O)O)C(=O)O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims 19
• 108020004707 nucleic acids Proteins 0.000 claims 15
• 102000039446 nucleic acids Human genes 0.000 claims 15
• 150000007523 nucleic acids Chemical class 0.000 claims 15
• 238000000034 method Methods 0.000 claims 9
• 125000000539 amino acid group Chemical group 0.000 claims 8
• 150000001413 amino acids Chemical group 0.000 claims 8
• 239000002773 nucleotide Substances 0.000 claims 8
• 125000003729 nucleotide group Chemical group 0.000 claims 8
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 6
• 108091034117 Oligonucleotide Proteins 0.000 claims 4
• 235000003704 aspartic acid Nutrition 0.000 claims 4
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims 4
• 238000006243 chemical reaction Methods 0.000 claims 4
• 239000013615 primer Substances 0.000 claims 4
• 239000011541 reaction mixture Substances 0.000 claims 4
• 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 claims 3
• 239000013604 expression vector Substances 0.000 claims 3
• 239000001963 growth medium Substances 0.000 claims 3
• 239000003155 DNA primer Substances 0.000 claims 2
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
• 235000004279 alanine Nutrition 0.000 claims 2
• 230000003321 amplification Effects 0.000 claims 2
• 238000000137 annealing Methods 0.000 claims 2
• 230000000694 effects Effects 0.000 claims 2
• 239000000203 mixture Substances 0.000 claims 2
• 238000003199 nucleic acid amplification method Methods 0.000 claims 2
• 238000006116 polymerization reaction Methods 0.000 claims 2
• 241000700605 Viruses Species 0.000 claims 1
• 239000007853 buffer solution Substances 0.000 claims 1
• 238000012258 culturing Methods 0.000 claims 1
• 238000004519 manufacturing process Methods 0.000 claims 1
• 108091033319 polynucleotide Proteins 0.000 claims 1
• 102000040430 polynucleotide Human genes 0.000 claims 1
• 239000002157 polynucleotide Substances 0.000 claims 1
• 235000018102 proteins Nutrition 0.000 claims 1
• 102000004169 proteins and genes Human genes 0.000 claims 1
• 108090000623 proteins and genes Proteins 0.000 claims 1
• 238000003259 recombinant expression Methods 0.000 claims 1
• 230000001105 regulatory effect Effects 0.000 claims 1
• 238000012163 sequencing technique Methods 0.000 claims 1
• 239000011780 sodium chloride Substances 0.000 claims 1
• 239000006228 supernatant Substances 0.000 claims 1
• 238000013518 transcription Methods 0.000 claims 1
• 230000035897 transcription Effects 0.000 claims 1