KR102594174B1 - Dna 중합효소 - Google Patents

Dna 중합효소 Download PDF

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Publication number
KR102594174B1
KR102594174B1 KR1020207020080A KR20207020080A KR102594174B1 KR 102594174 B1 KR102594174 B1 KR 102594174B1 KR 1020207020080 A KR1020207020080 A KR 1020207020080A KR 20207020080 A KR20207020080 A KR 20207020080A KR 102594174 B1 KR102594174 B1 KR 102594174B1
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dna polymerase
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ala
glu
ile
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Korean (ko)
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KR20200108283A (ko
Inventor
아틀레 노라프 라센
이본 표트롭스키
Original Assignee
유니벨시테테트 이 트롬소 - 노르게스 알크티스키 유니벨시테트
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
    • Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020207020080A 2017-12-15 2018-12-17 Dna 중합효소 Active KR102594174B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1721053.5A GB201721053D0 (en) 2017-12-15 2017-12-15 DNA polymerases
GB1721053.5 2017-12-15
PCT/EP2018/085342 WO2019115834A1 (en) 2017-12-15 2018-12-17 Dna polymerases

Publications (2)

Publication Number Publication Date
KR20200108283A KR20200108283A (ko) 2020-09-17
KR102594174B1 true KR102594174B1 (ko) 2023-10-25

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020207020080A Active KR102594174B1 (ko) 2017-12-15 2018-12-17 Dna 중합효소

Country Status (11)

Country Link
US (2) US11390857B2 (https=)
EP (1) EP3724349A1 (https=)
JP (1) JP7361694B2 (https=)
KR (1) KR102594174B1 (https=)
CN (1) CN111433373B (https=)
AU (1) AU2018382619B2 (https=)
BR (1) BR112020011605A2 (https=)
CA (1) CA3085558C (https=)
GB (1) GB201721053D0 (https=)
SG (1) SG11202004757RA (https=)
WO (1) WO2019115834A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022271682A1 (en) * 2021-06-21 2022-12-29 Amazon Technologies, Inc. Polymerases for isothermal nucleic acid amplification
CN120265762A (zh) * 2022-12-28 2025-07-04 深圳华大生命科学研究院 具有dna聚合酶活性的多肽及其应用
CN116103263A (zh) * 2023-02-13 2023-05-12 安序源生物科技(深圳)有限公司 突变型pwt聚合酶及其制备方法与应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100269979B1 (ko) 1998-04-15 2000-11-01 박한오 써무스 필리포미스로부터 분리된 내열성 dna 중합효소의 유전자 및 그로부터 유추되는 아미노산 서열
WO2015048763A1 (en) 2013-09-30 2015-04-02 Life Technologies Corporation Polymerase compositions, methods of making and using same

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6818431B1 (en) 1995-10-18 2004-11-16 Shanghai Mendel Dna Center Co. Ltd. DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
US6165765A (en) 1995-10-18 2000-12-26 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
ATE371022T1 (de) * 1998-06-17 2007-09-15 Ge Healthcare Bio Sciences Fy7 polymerase
JP2003507072A (ja) * 1999-08-21 2003-02-25 アマシャム・バイオサイエンス・コーポレイション 耐塩性を改善した、Ｅ６８１にアミノ酸置換を有するＴａｑＤＮＡポリメラーゼおよびその同族体
US20080311626A1 (en) 2004-09-17 2008-12-18 Arkea Hf Dna Polymerases Having Strand Displacement Activity
WO2006030455A1 (en) 2004-09-17 2006-03-23 Prokaria Ehf. Dna polymerases having strand displacement activity
CA2624324A1 (en) * 2005-10-06 2007-04-19 Lucigen Corporation Thermostable viral polymerases and methods of use
US20100047862A1 (en) * 2005-10-20 2010-02-25 Yoshihide Hayashizaki Novel DNA Polymerase
EP2539471B1 (en) 2010-02-26 2014-08-06 Life Technologies Corporation Method for sequencing using a modified polymerase
US20130040365A1 (en) * 2011-08-10 2013-02-14 Life Technologies Corporation Polymerase compositions, methods of making and using same
US8993298B1 (en) 2012-08-31 2015-03-31 New England Biolabs, Inc. DNA polymerases
GB201414745D0 (en) * 2014-08-19 2014-10-01 Articzymes As Exonucleases
WO2016077324A1 (en) * 2014-11-11 2016-05-19 Life Technologies Corporation Thiolated nucleotide analogues for nucleic acid synthesis
US11091745B2 (en) 2015-05-12 2021-08-17 Dna Polymerase Technology, Inc. Mutant polymerases and uses thereof
EP3356557B1 (en) * 2015-10-01 2022-07-20 Life Technologies Corporation Polymerase compositions and kits, and methods of using and making the same
CN108473970B (zh) * 2015-11-27 2022-09-13 国立大学法人九州大学 Dna聚合酶变体
CN108699539B (zh) 2016-02-29 2022-11-18 吉尼亚科技公司 外切核酸酶缺陷的聚合酶
GB201604876D0 (en) * 2016-03-22 2016-05-04 Uni I Tromsø Norges Arktiske Uni Marine DNA polymerases

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100269979B1 (ko) 1998-04-15 2000-11-01 박한오 써무스 필리포미스로부터 분리된 내열성 dna 중합효소의 유전자 및 그로부터 유추되는 아미노산 서열
WO2015048763A1 (en) 2013-09-30 2015-04-02 Life Technologies Corporation Polymerase compositions, methods of making and using same

Also Published As

Publication number Publication date
CN111433373B (zh) 2024-01-12
AU2018382619B2 (en) 2022-03-31
EP3724349A1 (en) 2020-10-21
CN111433373A (zh) 2020-07-17
AU2018382619A1 (en) 2020-06-11
US20230034466A1 (en) 2023-02-02
CA3085558C (en) 2023-01-10
US20200325459A1 (en) 2020-10-15
SG11202004757RA (en) 2020-07-29
JP2021507694A (ja) 2021-02-25
US11390857B2 (en) 2022-07-19
JP7361694B2 (ja) 2023-10-16
BR112020011605A2 (pt) 2020-12-08
KR20200108283A (ko) 2020-09-17
WO2019115834A1 (en) 2019-06-20
GB201721053D0 (en) 2018-01-31
CA3085558A1 (en) 2019-06-20

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