JP2011224002A5 - - Google Patents

Info

Publication number

JP2011224002A5

JP2011224002A5 JP2011090825A JP2011090825A JP2011224002A5 JP 2011224002 A5 JP2011224002 A5 JP 2011224002A5 JP 2011090825 A JP2011090825 A JP 2011090825A JP 2011090825 A JP2011090825 A JP 2011090825A JP 2011224002 A5 JP2011224002 A5 JP 2011224002A5

Authority

JP

Japan

Prior art keywords

mutant

seq

rna polymerase

variant

polypeptide

Prior art date

2011-04-15

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

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• 150000001413 amino acids Chemical class 0.000 claims 12
• 229920001184 polypeptide Polymers 0.000 claims 9
• 102000004196 processed proteins & peptides Human genes 0.000 claims 9
• 108090000765 processed proteins & peptides Proteins 0.000 claims 9
• 101710137500 T7 RNA polymerase Proteins 0.000 claims 7
• 108020004414 DNA Proteins 0.000 claims 6
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 6
• 238000000034 method Methods 0.000 claims 6
• 238000006467 substitution reaction Methods 0.000 claims 6
• 108020004707 nucleic acids Proteins 0.000 claims 4
• 102000039446 nucleic acids Human genes 0.000 claims 4
• 150000007523 nucleic acids Chemical class 0.000 claims 4
• 239000002773 nucleotide Substances 0.000 claims 4
• 125000003729 nucleotide group Chemical group 0.000 claims 4
• LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims 4
• 239000002342 ribonucleoside Substances 0.000 claims 3
• 239000001226 triphosphate Substances 0.000 claims 3
• 235000011178 triphosphate Nutrition 0.000 claims 3
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
• 102000053602 DNA Human genes 0.000 claims 2
• 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims 2
• 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims 2
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 2
• NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 claims 2
• 229920001213 Polysorbate 20 Polymers 0.000 claims 2
• 239000007853 buffer solution Substances 0.000 claims 2
• 230000001419 dependent effect Effects 0.000 claims 2
• 230000000694 effects Effects 0.000 claims 2
• DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 claims 2
• 239000013604 expression vector Substances 0.000 claims 2
• 239000000203 mixture Substances 0.000 claims 2
• 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims 2
• 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims 2
• 229910000160 potassium phosphate Inorganic materials 0.000 claims 2
• 235000011009 potassium phosphates Nutrition 0.000 claims 2
• 102200036370 rs139105452 Human genes 0.000 claims 2
• 102200065848 rs886042557 Human genes 0.000 claims 2
• UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 2
• DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims 2
• 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
• 125000003275 alpha amino acid group Chemical group 0.000 claims 1
• 239000000872 buffer Substances 0.000 claims 1
• 238000004519 manufacturing process Methods 0.000 claims 1
• 230000035772 mutation Effects 0.000 claims 1
• 238000003259 recombinant expression Methods 0.000 claims 1
• -1 ribonucleoside triphosphates Chemical class 0.000 claims 1
• 230000002194 synthesizing effect Effects 0.000 claims 1
• 238000013518 transcription Methods 0.000 claims 1
• 230000035897 transcription Effects 0.000 claims 1