JP2011224002A5 - - Google Patents
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- Publication number
- JP2011224002A5 JP2011224002A5 JP2011090825A JP2011090825A JP2011224002A5 JP 2011224002 A5 JP2011224002 A5 JP 2011224002A5 JP 2011090825 A JP2011090825 A JP 2011090825A JP 2011090825 A JP2011090825 A JP 2011090825A JP 2011224002 A5 JP2011224002 A5 JP 2011224002A5
- Authority
- JP
- Japan
- Prior art keywords
- mutant
- seq
- rna polymerase
- variant
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000001413 amino acids Chemical class 0.000 claims 12
- 229920001184 polypeptide Polymers 0.000 claims 9
- 102000004196 processed proteins & peptides Human genes 0.000 claims 9
- 108090000765 processed proteins & peptides Proteins 0.000 claims 9
- 101710137500 T7 RNA polymerase Proteins 0.000 claims 7
- 108020004414 DNA Proteins 0.000 claims 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 6
- 238000000034 method Methods 0.000 claims 6
- 238000006467 substitution reaction Methods 0.000 claims 6
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 239000002773 nucleotide Substances 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims 4
- 239000002342 ribonucleoside Substances 0.000 claims 3
- 239000001226 triphosphate Substances 0.000 claims 3
- 235000011178 triphosphate Nutrition 0.000 claims 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- 102000053602 DNA Human genes 0.000 claims 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 claims 2
- 229920001213 Polysorbate 20 Polymers 0.000 claims 2
- 239000007853 buffer solution Substances 0.000 claims 2
- 230000001419 dependent effect Effects 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims 2
- 229910000160 potassium phosphate Inorganic materials 0.000 claims 2
- 235000011009 potassium phosphates Nutrition 0.000 claims 2
- 102200036370 rs139105452 Human genes 0.000 claims 2
- 102200065848 rs886042557 Human genes 0.000 claims 2
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 238000003259 recombinant expression Methods 0.000 claims 1
- -1 ribonucleoside triphosphates Chemical class 0.000 claims 1
- 230000002194 synthesizing effect Effects 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10004059 | 2010-04-16 | ||
| EP10004059.1 | 2010-04-16 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2011224002A JP2011224002A (ja) | 2011-11-10 |
| JP2011224002A5 true JP2011224002A5 (https=) | 2014-05-29 |
| JP5851709B2 JP5851709B2 (ja) | 2016-02-03 |
Family
ID=42460013
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011090825A Expired - Fee Related JP5851709B2 (ja) | 2010-04-16 | 2011-04-15 | 増強された熱安定性を有する新規なｔ７ｒｎａポリメラーゼ変異体 |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US9193959B2 (https=) |
| EP (1) | EP2377928A3 (https=) |
| JP (1) | JP5851709B2 (https=) |
| CN (1) | CN102220294B (https=) |
| CA (1) | CA2737014A1 (https=) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2809284C (en) | 2010-08-31 | 2021-02-23 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways through protease manipulation |
| US9045740B2 (en) * | 2012-02-24 | 2015-06-02 | University Of Massachusetts | Modified T7-related RNA polymerases and methods of use thereof |
| BR112016002494A2 (pt) | 2013-08-05 | 2017-09-05 | Greenlight Biosciences Inc | Proteí-nas construídas com um sítio de clivagem de protease, ácido nucléico, vetor, célula e processo de engenharia de uma proteína recombinante e de um grande número de variantes de ácidos nucleicos que codificam proteínas recombinantes |
| KR20180002636A (ko) | 2015-03-30 | 2018-01-08 | 그린라이트 바이오사이언시스, 아이엔씨. | 리보핵산의 무세포 생산 |
| EP3882343A3 (en) | 2016-01-13 | 2021-12-08 | New England Biolabs, Inc. | Thermostable variants of t7 rna polymerase |
| CR20180525A (es) | 2016-04-06 | 2019-02-14 | Greenlight Biosciences Inc | Producción de ácido ribonucleico libre de células |
| CN107460177B (zh) * | 2016-06-06 | 2021-08-17 | 张海生 | 可利用化学修饰核苷酸的rna聚合酶突变体 |
| WO2018111967A1 (en) | 2016-12-13 | 2018-06-21 | Modernatx, Inc. | Rna affinity purification |
| US10034951B1 (en) * | 2017-06-21 | 2018-07-31 | New England Biolabs, Inc. | Use of thermostable RNA polymerases to produce RNAs having reduced immunogenicity |
| CN111417724A (zh) | 2017-06-30 | 2020-07-14 | 科德克希思公司 | T7 rna聚合酶变体 |
| US10793841B2 (en) | 2017-06-30 | 2020-10-06 | Codexis, Inc. | T7 RNA polymerase variants |
| ES2983060T3 (es) | 2017-08-18 | 2024-10-21 | Modernatx Inc | Variantes de ARN polimerasa |
| BR112020007068A2 (pt) | 2017-10-11 | 2020-10-06 | Greenlight Biosciences, Inc. | métodos e composições para produção de nucleosídeo trifosfato e ácido ribonucleico |
| ES2969883T3 (es) * | 2018-03-21 | 2024-05-23 | Hoffmann La Roche | ADN polimerasas para la incorporación eficiente y eficaz de dNTP metilados |
| JP2021520815A (ja) * | 2018-04-10 | 2021-08-26 | グリーンライト バイオサイエンシーズ インコーポレーテッドＧｒｅｅｎｌｉｇｈｔ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． | Ｔ７ｒｎａポリメラーゼバリアント |
| SG11202011240YA (en) | 2018-06-26 | 2020-12-30 | Broad Inst Inc | Crispr/cas and transposase based amplification compositions, systems and methods |
| US11851694B1 (en) | 2019-02-20 | 2023-12-26 | Modernatx, Inc. | High fidelity in vitro transcription |
| CN113795579A (zh) | 2019-02-20 | 2021-12-14 | 摩登纳特斯有限公司 | 用于共转录加帽的rna聚合酶变体 |
| IL300418B2 (en) * | 2020-08-06 | 2024-11-01 | Univ Tsinghua | Chemical synthesis of large and mirror-image proteins and uses thereof |
| EP4232569B1 (en) | 2020-10-23 | 2026-03-11 | LineaRX, Inc. | Compositions and methods for rna synthesis |
| CN112980811B (zh) * | 2021-03-06 | 2022-02-25 | 苏州瀚源新酶生物科技有限公司 | Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用 |
| US20220363937A1 (en) | 2021-05-14 | 2022-11-17 | Armstrong World Industries, Inc. | Stabilization of antimicrobial coatings |
| CN115960247A (zh) * | 2021-10-13 | 2023-04-14 | 苏州科锐迈德生物医药科技有限公司 | 基于t7rna聚合酶的融合多肽及其制备方法、用途 |
| CN114774453B (zh) * | 2022-03-14 | 2023-04-21 | 湖北大学 | 一种运动发酵单胞菌基因表达严谨调控系统的构建方法和应用 |
| CN121046349A (zh) * | 2023-07-05 | 2025-12-02 | 上海天鹜科技有限公司 | 一种具有高热稳定性的t7 rna聚合酶突变体及其应用 |
| WO2025142849A1 (ja) * | 2023-12-26 | 2025-07-03 | 東洋紡株式会社 | 改変型ｒｎａポリメラーゼ |
| CN120082531A (zh) * | 2023-12-28 | 2025-06-03 | 翌圣生物科技(上海)股份有限公司 | T7 rna聚合酶突变体及其应用 |
| US12492420B2 (en) | 2024-03-29 | 2025-12-09 | New England Biolabs, Inc. | Compositions, kits, and methods for in vitro transcription |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4952496A (en) | 1984-03-30 | 1990-08-28 | Associated Universities, Inc. | Cloning and expression of the gene for bacteriophage T7 RNA polymerase |
| US5385834A (en) * | 1993-08-13 | 1995-01-31 | Georgia Tech Research Corporation | Mutant T7 RNA polymerase GP1(lys222) exhibiting altered promoter recognition |
| CA2353792C (en) | 1998-12-11 | 2007-11-27 | Akzo Nobel N.V. | Mutants of rna polymerases with increased stability |
| JP4399684B2 (ja) * | 1999-08-17 | 2010-01-20 | 東洋紡績株式会社 | 改良されたｒｎａポリメラーゼ |
| EP1261696B1 (en) | 2000-03-07 | 2005-05-04 | bioMerieux B.V. | Rna polymerase mutants with increased thermostability |
| JP2003061683A (ja) * | 2001-06-11 | 2003-03-04 | Nippon Genetech Co Ltd | 変異型ｒｎａポリメラーゼ |
| WO2009113718A1 (ja) | 2008-03-11 | 2009-09-17 | 東ソー株式会社 | 機能改善したｒｎａポリメラーゼ変異体 |
| CN102177236B (zh) * | 2008-08-08 | 2013-11-06 | 东曹株式会社 | 功能改善的rna聚合酶突变体 |
| JP5789949B2 (ja) * | 2010-10-05 | 2015-10-07 | 東ソー株式会社 | 改良されたｒｎａポリメラーゼ変異体 |
-
2011
- 2011-03-23 US US13/069,514 patent/US9193959B2/en not_active Expired - Fee Related
- 2011-04-12 CA CA2737014A patent/CA2737014A1/en not_active Abandoned
- 2011-04-14 EP EP11162482A patent/EP2377928A3/en not_active Withdrawn
- 2011-04-15 JP JP2011090825A patent/JP5851709B2/ja not_active Expired - Fee Related
- 2011-04-15 CN CN201110094968.1A patent/CN102220294B/zh not_active Expired - Fee Related
-
2015
- 2015-10-16 US US14/885,732 patent/US20160032261A1/en not_active Abandoned
- 2015-10-16 US US14/885,727 patent/US20160032260A1/en not_active Abandoned
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