CN102220294B - 具有增强的热稳定性的新颖的t7 rna聚合酶变体 - Google Patents

具有增强的热稳定性的新颖的t7 rna聚合酶变体 Download PDF

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CN102220294B
CN102220294B CN201110094968.1A CN201110094968A CN102220294B CN 102220294 B CN102220294 B CN 102220294B CN 201110094968 A CN201110094968 A CN 201110094968A CN 102220294 B CN102220294 B CN 102220294B
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glu
lys
val
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CN102220294A (zh
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M·格赖夫
R·米勒
C·鲁道夫
A·鲁夫
M·施密特
H·佐贝克
J-P·塔尔霍费尔
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201110094968.1A 2010-04-16 2011-04-15 具有增强的热稳定性的新颖的t7 rna聚合酶变体 Expired - Fee Related CN102220294B (zh)

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EP10004059 2010-04-16
EP10004059.1 2010-04-16

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CN102220294A CN102220294A (zh) 2011-10-19
CN102220294B true CN102220294B (zh) 2014-11-12

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US (3) US9193959B2 (https=)
EP (1) EP2377928A3 (https=)
JP (1) JP5851709B2 (https=)
CN (1) CN102220294B (https=)
CA (1) CA2737014A1 (https=)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2809284C (en) 2010-08-31 2021-02-23 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
US9045740B2 (en) * 2012-02-24 2015-06-02 University Of Massachusetts Modified T7-related RNA polymerases and methods of use thereof
BR112016002494A2 (pt) 2013-08-05 2017-09-05 Greenlight Biosciences Inc Proteí-nas construídas com um sítio de clivagem de protease, ácido nucléico, vetor, célula e processo de engenharia de uma proteína recombinante e de um grande número de variantes de ácidos nucleicos que codificam proteínas recombinantes
KR20180002636A (ko) 2015-03-30 2018-01-08 그린라이트 바이오사이언시스, 아이엔씨. 리보핵산의 무세포 생산
EP3882343A3 (en) 2016-01-13 2021-12-08 New England Biolabs, Inc. Thermostable variants of t7 rna polymerase
CR20180525A (es) 2016-04-06 2019-02-14 Greenlight Biosciences Inc Producción de ácido ribonucleico libre de células
CN107460177B (zh) * 2016-06-06 2021-08-17 张海生 可利用化学修饰核苷酸的rna聚合酶突变体
WO2018111967A1 (en) 2016-12-13 2018-06-21 Modernatx, Inc. Rna affinity purification
US10034951B1 (en) * 2017-06-21 2018-07-31 New England Biolabs, Inc. Use of thermostable RNA polymerases to produce RNAs having reduced immunogenicity
CN111417724A (zh) 2017-06-30 2020-07-14 科德克希思公司 T7 rna聚合酶变体
US10793841B2 (en) 2017-06-30 2020-10-06 Codexis, Inc. T7 RNA polymerase variants
ES2983060T3 (es) 2017-08-18 2024-10-21 Modernatx Inc Variantes de ARN polimerasa
BR112020007068A2 (pt) 2017-10-11 2020-10-06 Greenlight Biosciences, Inc. métodos e composições para produção de nucleosídeo trifosfato e ácido ribonucleico
ES2969883T3 (es) * 2018-03-21 2024-05-23 Hoffmann La Roche ADN polimerasas para la incorporación eficiente y eficaz de dNTP metilados
JP2021520815A (ja) * 2018-04-10 2021-08-26 グリーンライト バイオサイエンシーズ インコーポレーテッドＧｒｅｅｎｌｉｇｈｔ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． Ｔ７ｒｎａポリメラーゼバリアント
SG11202011240YA (en) 2018-06-26 2020-12-30 Broad Inst Inc Crispr/cas and transposase based amplification compositions, systems and methods
US11851694B1 (en) 2019-02-20 2023-12-26 Modernatx, Inc. High fidelity in vitro transcription
CN113795579A (zh) 2019-02-20 2021-12-14 摩登纳特斯有限公司 用于共转录加帽的rna聚合酶变体
IL300418B2 (en) * 2020-08-06 2024-11-01 Univ Tsinghua Chemical synthesis of large and mirror-image proteins and uses thereof
EP4232569B1 (en) 2020-10-23 2026-03-11 LineaRX, Inc. Compositions and methods for rna synthesis
CN112980811B (zh) * 2021-03-06 2022-02-25 苏州瀚源新酶生物科技有限公司 Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用
US20220363937A1 (en) 2021-05-14 2022-11-17 Armstrong World Industries, Inc. Stabilization of antimicrobial coatings
CN115960247A (zh) * 2021-10-13 2023-04-14 苏州科锐迈德生物医药科技有限公司 基于t7rna聚合酶的融合多肽及其制备方法、用途
CN114774453B (zh) * 2022-03-14 2023-04-21 湖北大学 一种运动发酵单胞菌基因表达严谨调控系统的构建方法和应用
CN121046349A (zh) * 2023-07-05 2025-12-02 上海天鹜科技有限公司 一种具有高热稳定性的t7 rna聚合酶突变体及其应用
WO2025142849A1 (ja) * 2023-12-26 2025-07-03 東洋紡株式会社 改変型ｒｎａポリメラーゼ
CN120082531A (zh) * 2023-12-28 2025-06-03 翌圣生物科技(上海)股份有限公司 T7 rna聚合酶突变体及其应用
US12492420B2 (en) 2024-03-29 2025-12-09 New England Biolabs, Inc. Compositions, kits, and methods for in vitro transcription

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000036112A1 (en) * 1998-12-11 2000-06-22 Akzo Nobel N.V. Mutants of rna polymerases with increased stability
WO2001066705A1 (en) * 2000-03-07 2001-09-13 Akzo Nobel N.V. Rna polymerase mutants with increased thermostability
WO2009113718A1 (ja) * 2008-03-11 2009-09-17 東ソー株式会社 機能改善したｒｎａポリメラーゼ変異体

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952496A (en) 1984-03-30 1990-08-28 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
US5385834A (en) * 1993-08-13 1995-01-31 Georgia Tech Research Corporation Mutant T7 RNA polymerase GP1(lys222) exhibiting altered promoter recognition
JP4399684B2 (ja) * 1999-08-17 2010-01-20 東洋紡績株式会社 改良されたｒｎａポリメラーゼ
JP2003061683A (ja) * 2001-06-11 2003-03-04 Nippon Genetech Co Ltd 変異型ｒｎａポリメラーゼ
CN102177236B (zh) * 2008-08-08 2013-11-06 东曹株式会社 功能改善的rna聚合酶突变体
JP5789949B2 (ja) * 2010-10-05 2015-10-07 東ソー株式会社 改良されたｒｎａポリメラーゼ変異体

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000036112A1 (en) * 1998-12-11 2000-06-22 Akzo Nobel N.V. Mutants of rna polymerases with increased stability
WO2001066705A1 (en) * 2000-03-07 2001-09-13 Akzo Nobel N.V. Rna polymerase mutants with increased thermostability
WO2009113718A1 (ja) * 2008-03-11 2009-09-17 東ソー株式会社 機能改善したｒｎａポリメラーゼ変異体

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JP5851709B2 (ja) 2016-02-03
CA2737014A1 (en) 2011-10-16
EP2377928A3 (en) 2012-06-27
US20160032261A1 (en) 2016-02-04
US20110256589A1 (en) 2011-10-20
EP2377928A2 (en) 2011-10-19
CN102220294A (zh) 2011-10-19
US9193959B2 (en) 2015-11-24
JP2011224002A (ja) 2011-11-10
US20160032260A1 (en) 2016-02-04
HK1163165A1 (en) 2012-09-07

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