JP2019530474A5 - - Google Patents
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- Publication number
- JP2019530474A5 JP2019530474A5 JP2019535206A JP2019535206A JP2019530474A5 JP 2019530474 A5 JP2019530474 A5 JP 2019530474A5 JP 2019535206 A JP2019535206 A JP 2019535206A JP 2019535206 A JP2019535206 A JP 2019535206A JP 2019530474 A5 JP2019530474 A5 JP 2019530474A5
- Authority
- JP
- Japan
- Prior art keywords
- amino acid
- glucan
- unnatural
- leu
- glucosyltransferase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000000340 Glucosyltransferases Human genes 0.000 claims description 42
- 108010055629 Glucosyltransferases Proteins 0.000 claims description 42
- 238000006467 substitution reaction Methods 0.000 claims description 25
- 229920000310 Alpha glucan Polymers 0.000 claims description 24
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 24
- 125000000539 amino acid group Chemical group 0.000 claims description 23
- 102000040430 polynucleotide Human genes 0.000 claims description 14
- 108091033319 polynucleotide Proteins 0.000 claims description 14
- 239000002157 polynucleotide Substances 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 10
- DXALOGXSFLZLLN-WTZPKTTFSA-N (3s,4s,5r)-1,3,4,6-tetrahydroxy-5-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyhexan-2-one Chemical compound OCC(=O)[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O DXALOGXSFLZLLN-WTZPKTTFSA-N 0.000 claims description 9
- JPFGFRMPGVDDGE-UHFFFAOYSA-N Leucrose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)(CO)OC1 JPFGFRMPGVDDGE-UHFFFAOYSA-N 0.000 claims description 9
- 229930006000 Sucrose Natural products 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 239000002773 nucleotide Substances 0.000 claims description 4
- 125000003729 nucleotide group Chemical group 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 239000005720 sucrose Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 229920005640 poly alpha-1,3-glucan Polymers 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000000126 in silico method Methods 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000000734 protein sequencing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662394354P | 2016-09-14 | 2016-09-14 | |
| US62/394,354 | 2016-09-14 | ||
| PCT/US2017/051279 WO2018052942A1 (en) | 2016-09-14 | 2017-09-13 | Engineered glucosyltransferases |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019530474A JP2019530474A (ja) | 2019-10-24 |
| JP2019530474A5 true JP2019530474A5 (https=) | 2020-10-01 |
| JP7136783B2 JP7136783B2 (ja) | 2022-09-13 |
Family
ID=59969237
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019535206A Active JP7136783B2 (ja) | 2016-09-14 | 2017-09-13 | 遺伝子操作されたグルコシルトランスフェラーゼ |
Country Status (9)
| Country | Link |
|---|---|
| US (4) | US10301604B2 (https=) |
| EP (1) | EP3512955B1 (https=) |
| JP (1) | JP7136783B2 (https=) |
| CN (1) | CN109715818A (https=) |
| AU (1) | AU2017326168B2 (https=) |
| BR (1) | BR112019004213A2 (https=) |
| CA (1) | CA3033369A1 (https=) |
| FI (1) | FI3512955T3 (https=) |
| WO (1) | WO2018052942A1 (https=) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3630993A1 (en) | 2017-05-23 | 2020-04-08 | DuPont Industrial Biosciences USA, LLC | Enzymatic production of alpha-1,3-glucan |
| US10865393B2 (en) | 2017-09-13 | 2020-12-15 | Dupont Industrial Biosciences Usa, Llc | Engineered glucosyltransferases |
| EP3682003A1 (en) | 2017-09-13 | 2020-07-22 | DuPont Industrial Biosciences USA, LLC | Engineered glucosyltransferases |
| JP7343490B2 (ja) | 2017-10-13 | 2023-09-12 | ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド | 流動性バルク粒状多糖 |
| US11098334B2 (en) | 2017-12-14 | 2021-08-24 | Nutrition & Biosciences USA 4, Inc. | Alpha-1,3-glucan graft copolymers |
| CN112368374A (zh) * | 2018-03-09 | 2021-02-12 | 营养与生物科学美国4公司 | 工程化的葡糖基转移酶 |
| CN112638163A (zh) * | 2018-07-05 | 2021-04-09 | 杜邦营养生物科学有限公司 | 葡糖基转移酶在发酵奶基产品中提供改善的质地的用途 |
| EP3870616B1 (en) | 2018-10-25 | 2023-10-11 | DuPont Industrial Biosciences USA, LLC | Alpha-1,3-glucan graft copolymers |
| US20220033531A1 (en) | 2018-12-17 | 2022-02-03 | Dupont Industrial Biosciences Usa,Llc | Polysaccharide Derivatives and Compositions Comprising Same |
| US20220267745A1 (en) * | 2019-07-09 | 2022-08-25 | Nutrition & Biosciences USA 4, Inc. | Engineered alpha-1,3 branching enzymes |
| EP4013241A1 (en) | 2019-08-16 | 2022-06-22 | Nutrition & Biosciences USA 4, Inc. | Flour- and meal-based food products comprising insoluble alpha-1,3-glucan |
| JP7849032B2 (ja) | 2019-11-06 | 2026-04-21 | ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド | 高結晶質アルファ－１，３－グルカン |
| CN115052905B (zh) | 2020-02-04 | 2024-06-11 | 营养与生物科学美国4公司 | 包含α-1,3糖苷键的不溶性α-葡聚糖的水性分散体 |
| JP2023528442A (ja) | 2020-06-04 | 2023-07-04 | ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド | デキストラン－α－グルカングラフトコポリマー及びその誘導体 |
| WO2021252569A1 (en) | 2020-06-10 | 2021-12-16 | Nutrition & Biosciences USA 4, Inc. | Poly alpha-1,6-glucan derivatives and compositions comprising same |
| EP4294848A1 (en) | 2021-02-19 | 2023-12-27 | Nutrition & Biosciences USA 4, Inc. | Oxidized polysaccharide derivatives |
| WO2022235735A1 (en) | 2021-05-04 | 2022-11-10 | Nutrition & Biosciences USA 4, Inc. | Compositions comprising oxidized insoluble alpha-glucan |
| CN117242102A (zh) | 2021-05-04 | 2023-12-15 | 营养与生物科学美国4公司 | 包含不溶性α-葡聚糖的组合物 |
| JP2024525685A (ja) | 2021-07-13 | 2024-07-12 | ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド | カチオン性グルカンエステル誘導体 |
| EP4408197A1 (en) | 2021-09-30 | 2024-08-07 | International N&H Denmark ApS | Method for reducing sugar in food stuff |
| EP4426362A1 (en) | 2021-11-05 | 2024-09-11 | Nutrition & Biosciences USA 4, Inc. | Glucan derivatives for microbial control |
| EP4463519A1 (en) | 2022-01-12 | 2024-11-20 | Nutrition & Biosciences USA 4, Inc. | Coating compositions comprising rubber and insoluble alpha-glucan |
| EP4496834A1 (en) | 2022-03-21 | 2025-01-29 | Nutrition & Biosciences USA 4, Inc. | Compositions comprising insoluble alpha-glucan |
| CN114717213B (zh) * | 2022-05-05 | 2023-09-22 | 广西科学院 | 一种葡聚糖蔗糖酶的n端截短突变酶及其制备方法 |
| JP2025523007A (ja) | 2022-07-11 | 2025-07-17 | ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド | 両親媒性グルカンエステル誘導体 |
| WO2024086560A1 (en) | 2022-10-17 | 2024-04-25 | International N&H Denmark Aps | Method for improving flavor in plant-based food stuff |
| US20250339361A1 (en) | 2022-11-02 | 2025-11-06 | Nutrition & Biosciences USA 4, Inc. | Compositions comprising xanthan gum and crystalline alpha-1,3-glucan |
| AU2024242918A1 (en) | 2023-03-29 | 2025-09-18 | Danisco Us Inc. | Methods for modifying texture in foodstuff via preferably in situ produced alpha-glucan |
| CN121398902A (zh) | 2023-06-30 | 2026-01-23 | 营养与生物科学美国第四公司 | 多孔α-1,3-葡聚糖组合物 |
| WO2025072419A1 (en) | 2023-09-29 | 2025-04-03 | Nutrition & Biosciences Usa 1, Llc | Crosslinked alpha-glucan derivatives |
| WO2025072417A1 (en) | 2023-09-29 | 2025-04-03 | Nutrition & Biosciences USA 4, Inc. | Polysaccharide derivatives |
| WO2025072416A1 (en) | 2023-09-29 | 2025-04-03 | Nutrition & Biosciences USA 4, Inc. | Polysaccharide derivatives |
| WO2025198996A1 (en) | 2024-03-19 | 2025-09-25 | Danisco Us Inc. | Method for improving flavor in foodstuff |
| WO2026015598A2 (en) | 2024-07-11 | 2026-01-15 | International N&H Denmark Aps | Method to provide improved texture and stable sucrose levels in foodstuff with glucosyltransferase |
| WO2026024663A1 (en) | 2024-07-24 | 2026-01-29 | Nutrition & Biosciences USA 4, Inc. | Compositions comprising alpha-glucan with alpha-1,3 glycosidic linkages |
| WO2026039397A1 (en) | 2024-08-13 | 2026-02-19 | Nutrition & Biosciences USA 4, Inc. | Alpha-glucan graft copolymer derivatives |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1001736A1 (en) | 1996-10-11 | 2000-05-24 | Novo Nordisk A/S | STARCH BINDING DOMAINS (SBDs) FOR ORAL CARE PRODUCTS |
| US5952205A (en) | 1998-02-06 | 1999-09-14 | Neose Technologies, Inc. | Process for processing sucrose into glucose and fructose |
| JP2000175694A (ja) | 1998-12-17 | 2000-06-27 | Lion Corp | 酵素合成によるムタンの製造方法 |
| DE60009886T2 (de) | 1999-01-25 | 2005-03-31 | E.I. Du Pont De Nemours And Co., Wilmington | Polysaccharidfasern |
| US6867026B2 (en) | 2000-05-25 | 2005-03-15 | Nederlandse Organisatie Voor Toegepast Natuurwetenschappelijk Onderzoek Tno | Glucosyltransferases |
| JP3709435B2 (ja) * | 2001-10-03 | 2005-10-26 | 独立行政法人食品総合研究所 | 改変デキストランスクラーゼ、その遺伝子組み換え体、グルカンの製造法 |
| CA2480962A1 (en) * | 2002-03-07 | 2003-09-18 | The Forsyth Institute | Immunogenicity of glucan binding protein |
| US7780970B2 (en) | 2002-08-06 | 2010-08-24 | Danisco A/S | Composition |
| US7524645B2 (en) | 2004-12-14 | 2009-04-28 | Centre National De La Recherche Scientifique (Cnrs) | Fully active alternansucrases partially deleted in its carboxy-terminal and amino-terminal domains and mutants thereof |
| EP1679374A1 (en) | 2005-01-10 | 2006-07-12 | Bayer CropScience GmbH | Transformed plant expressing a mutansucrase and synthesizing a modified starch |
| FR2897069B1 (fr) | 2006-02-08 | 2012-06-08 | Centre Nat Rech Scient | Construction de nouveaux variants de l'enzyme dextrane-saccharase dsr-s par ingienerie moleculaire. |
| EP2100966A1 (en) | 2008-03-12 | 2009-09-16 | Institut Pasteur | Mutants of glycoside hydrolases and uses thereof for synthesizing complex oligosaccharides and disaccharide intermediates |
| JP5680860B2 (ja) * | 2009-01-30 | 2015-03-04 | サントリーホールディングス株式会社 | フラボノイド３位ガラクトース・グルコース転移酵素、及びそれをコードするポリヌクレオチド |
| JP2011206273A (ja) | 2010-03-30 | 2011-10-20 | Terumo Corp | 注射針組立体および薬剤注射装置 |
| CA2798118A1 (en) | 2010-05-04 | 2011-11-10 | Sanofi-Aventis Deutschland Gmbh | Tactile identification of drug filled cartridge |
| JP5015287B2 (ja) | 2010-05-11 | 2012-08-29 | シャープ株式会社 | 加熱調理器 |
| JP5851149B2 (ja) | 2011-08-08 | 2016-02-03 | 株式会社ニューフレアテクノロジー | 成膜装置および成膜方法 |
| US8642757B2 (en) | 2011-09-09 | 2014-02-04 | E I Du Pont De Nemours And Company | High titer production of highly linear poly (α 1,3 glucan) |
| US9080195B2 (en) | 2011-09-09 | 2015-07-14 | E I Du Pont De Nemours And Company | High titer production of poly (α 1,3 glucan) |
| WO2014052386A2 (en) | 2012-09-25 | 2014-04-03 | E. I. Du Pont De Nemours And Company | Glucosyltransferase enzymes for production of glucan polymers |
| CN104968781A (zh) * | 2012-12-24 | 2015-10-07 | 诺维信公司 | 具有内切葡聚糖酶活性的多肽以及对其进行编码的多核苷酸 |
| US9169506B2 (en) | 2013-09-05 | 2015-10-27 | E I Du Pont De Nemours And Company | Process for producing alpha-1,3-glucan polymer with reduced molecular weight |
| US9644190B2 (en) | 2014-02-06 | 2017-05-09 | The United States Of America, As Represented By The Secretary Of Agriculture | Modified glucansucrase and related methods |
| WO2015123327A1 (en) | 2014-02-14 | 2015-08-20 | E. I. Du Pont De Nemours And Company | Glucosyltransferase enzymes for production of glucan polymers |
| WO2015123323A1 (en) | 2014-02-14 | 2015-08-20 | E. I. Du Pont De Nemours And Company | Poly-alpha-1,3-1,6-glucans for viscosity modification |
| BR112016027867A2 (pt) | 2014-05-29 | 2017-10-24 | Du Pont | composições de fibra e de carboidrato, produto alimentício, métodos para produzir uma composição de fibra, de carboidrato, para reduzir o índice glicêmico e para inibir a elevação do nível de açúcar, composição cosmética, uso da composição de fibra e composição |
| MX2016015703A (es) | 2014-05-29 | 2017-03-16 | Du Pont | Sintesis enzimatica de fibra de glucano soluble. |
| WO2015183722A1 (en) | 2014-05-29 | 2015-12-03 | E. I. Du Pont De Nemours And Company | Enzymatic synthesis of soluble glucan fiber |
| BR112016027887A2 (pt) | 2014-05-29 | 2017-10-24 | Du Pont | composições de fibra e de carboidrato, produto alimentício, métodos para produzir uma composição de fibra, para produzir uma composição de carboidratos misturada, método para reduzir o índice glicêmico, método para inibir a elevação do nível de açúcar no sangue, composição cosmética, uso da composição e composição |
| US20170204442A1 (en) | 2014-05-29 | 2017-07-20 | E I Du Pont De Nemours And Company | Enzymatic synthesis of soluble glucan fiber |
| CA2949289A1 (en) | 2014-05-29 | 2015-12-03 | E. I. Du Pont De Nemours And Company | Enzymatic synthesis of soluble glucan fiber |
| US10059779B2 (en) | 2014-11-05 | 2018-08-28 | E I Du Pont De Nemours And Company | Enzymatically polymerized gelling dextrans |
| CA2989337A1 (en) | 2015-06-17 | 2016-12-22 | E I Du Pont De Nemours And Company | Glucosyltransferase amino acid sequence motifs for producing linear poly alpha-1,3-glucan |
-
2017
- 2017-09-13 FI FIEP17772809.4T patent/FI3512955T3/fi active
- 2017-09-13 US US15/702,893 patent/US10301604B2/en active Active
- 2017-09-13 CN CN201780055064.3A patent/CN109715818A/zh active Pending
- 2017-09-13 AU AU2017326168A patent/AU2017326168B2/en active Active
- 2017-09-13 CA CA3033369A patent/CA3033369A1/en not_active Abandoned
- 2017-09-13 JP JP2019535206A patent/JP7136783B2/ja active Active
- 2017-09-13 EP EP17772809.4A patent/EP3512955B1/en active Active
- 2017-09-13 BR BR112019004213A patent/BR112019004213A2/pt not_active IP Right Cessation
- 2017-09-13 WO PCT/US2017/051279 patent/WO2018052942A1/en not_active Ceased
-
2019
- 2019-04-11 US US16/381,010 patent/US11028373B2/en active Active
-
2021
- 2021-06-04 US US17/338,842 patent/US11952598B2/en active Active
-
2024
- 2024-04-05 US US18/628,140 patent/US12378535B2/en active Active
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