CN109715818A - 工程化的葡糖基转移酶 - Google Patents

工程化的葡糖基转移酶 Download PDF

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Publication number
CN109715818A
CN109715818A CN201780055064.3A CN201780055064A CN109715818A CN 109715818 A CN109715818 A CN 109715818A CN 201780055064 A CN201780055064 A CN 201780055064A CN 109715818 A CN109715818 A CN 109715818A
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China
Prior art keywords
amino acid
glucosyltransferase
seq
residue
natural
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201780055064.3A
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English (en)
Chinese (zh)
Inventor
李友根
S.M.轩尼诗
S.克拉尔
M.S.佩恩
V.阿尔坎
程琼
R.迪克西莫
E.D.森科
J.B.帕克
R.R.波特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DuPont Industrial Biosciences USA LLC
Nutrition and Biosciences USA 4 Inc
Original Assignee
EI Du Pont de Nemours and Co
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Filing date
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Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of CN109715818A publication Critical patent/CN109715818A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Materials Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201780055064.3A 2016-09-14 2017-09-13 工程化的葡糖基转移酶 Pending CN109715818A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662394354P 2016-09-14 2016-09-14
US62/394354 2016-09-14
PCT/US2017/051279 WO2018052942A1 (en) 2016-09-14 2017-09-13 Engineered glucosyltransferases

Publications (1)

Publication Number Publication Date
CN109715818A true CN109715818A (zh) 2019-05-03

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CN201780055064.3A Pending CN109715818A (zh) 2016-09-14 2017-09-13 工程化的葡糖基转移酶

Country Status (9)

Country Link
US (4) US10301604B2 (https=)
EP (1) EP3512955B1 (https=)
JP (1) JP7136783B2 (https=)
CN (1) CN109715818A (https=)
AU (1) AU2017326168B2 (https=)
BR (1) BR112019004213A2 (https=)
CA (1) CA3033369A1 (https=)
FI (1) FI3512955T3 (https=)
WO (1) WO2018052942A1 (https=)

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CN114717213A (zh) * 2022-05-05 2022-07-08 广西科学院 一种葡聚糖蔗糖酶的n端截短突变酶及其制备方法
CN115003805A (zh) * 2019-07-09 2022-09-02 营养与生物科学美国第四公司 工程化的α-1,3分支酶

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EP3870616B1 (en) 2018-10-25 2023-10-11 DuPont Industrial Biosciences USA, LLC Alpha-1,3-glucan graft copolymers
US20220033531A1 (en) 2018-12-17 2022-02-03 Dupont Industrial Biosciences Usa,Llc Polysaccharide Derivatives and Compositions Comprising Same
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WO2022178073A1 (en) 2021-02-19 2022-08-25 Nutrition & Biosciences USA 4, Inc. Polysaccharide derivatives for detergent compositions
EP4334364A1 (en) 2021-05-04 2024-03-13 Nutrition & Biosciences USA 4, Inc. Compositions comprising oxidized insoluble alpha-glucan
WO2022235655A1 (en) 2021-05-04 2022-11-10 Nutrition & Biosciences USA 4, Inc. Compositions comprising insoluble alpha-glucan
US20240307288A1 (en) 2021-07-13 2024-09-19 Nutrition & Biosciences USA 4, Inc. Cationic glucan ester derivatives
CN118510405A (zh) 2021-09-30 2024-08-16 国际营养与健康丹麦私人有限公司 降低食料中糖的方法
EP4426362A1 (en) 2021-11-05 2024-09-11 Nutrition & Biosciences USA 4, Inc. Glucan derivatives for microbial control
EP4463519A1 (en) 2022-01-12 2024-11-20 Nutrition & Biosciences USA 4, Inc. Coating compositions comprising rubber and insoluble alpha-glucan
KR20240167661A (ko) 2022-03-21 2024-11-27 뉴트리션 앤드 바이오사이언시스 유에스에이 4, 인크. 불용성 알파-글루칸을 포함하는 조성물
JP2025523007A (ja) 2022-07-11 2025-07-17 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド 両親媒性グルカンエステル誘導体
WO2024086560A1 (en) 2022-10-17 2024-04-25 International N&H Denmark Aps Method for improving flavor in plant-based food stuff
US20250339361A1 (en) 2022-11-02 2025-11-06 Nutrition & Biosciences USA 4, Inc. Compositions comprising xanthan gum and crystalline alpha-1,3-glucan
CN120957606A (zh) 2023-03-29 2025-11-14 国际营养与健康丹麦有限公司 经由优选原位产生的α-葡聚糖改变食料质地的方法
CN121398902A (zh) 2023-06-30 2026-01-23 营养与生物科学美国第四公司 多孔α-1,3-葡聚糖组合物
WO2025072419A1 (en) 2023-09-29 2025-04-03 Nutrition & Biosciences Usa 1, Llc Crosslinked alpha-glucan derivatives
WO2025072417A1 (en) 2023-09-29 2025-04-03 Nutrition & Biosciences USA 4, Inc. Polysaccharide derivatives
WO2025072416A1 (en) 2023-09-29 2025-04-03 Nutrition & Biosciences USA 4, Inc. Polysaccharide derivatives
WO2025198996A1 (en) 2024-03-19 2025-09-25 Danisco Us Inc. Method for improving flavor in foodstuff
WO2026015598A2 (en) 2024-07-11 2026-01-15 International N&H Denmark Aps Method to provide improved texture and stable sucrose levels in foodstuff with glucosyltransferase
WO2026024663A1 (en) 2024-07-24 2026-01-29 Nutrition & Biosciences USA 4, Inc. Compositions comprising alpha-glucan with alpha-1,3 glycosidic linkages
WO2026039397A1 (en) 2024-08-13 2026-02-19 Nutrition & Biosciences USA 4, Inc. Alpha-glucan graft copolymer derivatives

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115003805A (zh) * 2019-07-09 2022-09-02 营养与生物科学美国第四公司 工程化的α-1,3分支酶
CN114717213A (zh) * 2022-05-05 2022-07-08 广西科学院 一种葡聚糖蔗糖酶的n端截短突变酶及其制备方法
CN114717213B (zh) * 2022-05-05 2023-09-22 广西科学院 一种葡聚糖蔗糖酶的n端截短突变酶及其制备方法

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US20240417702A1 (en) 2024-12-19
EP3512955A1 (en) 2019-07-24
CA3033369A1 (en) 2018-03-22
US10301604B2 (en) 2019-05-28
FI3512955T3 (fi) 2023-07-18
EP3512955B1 (en) 2023-04-19
JP2019530474A (ja) 2019-10-24
US11952598B2 (en) 2024-04-09
US20190292528A1 (en) 2019-09-26
US11028373B2 (en) 2021-06-08
JP7136783B2 (ja) 2022-09-13
US20180072998A1 (en) 2018-03-15
AU2017326168A1 (en) 2019-02-21
AU2017326168B2 (en) 2022-02-24
US12378535B2 (en) 2025-08-05
BR112019004213A2 (pt) 2019-06-25
WO2018052942A1 (en) 2018-03-22
US20210395705A1 (en) 2021-12-23

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Application publication date: 20190503