FR2897069B1 - Construction de nouveaux variants de l'enzyme dextrane-saccharase dsr-s par ingienerie moleculaire. - Google Patents

Construction de nouveaux variants de l'enzyme dextrane-saccharase dsr-s par ingienerie moleculaire.

Info

Publication number
FR2897069B1
FR2897069B1 FR0601117A FR0601117A FR2897069B1 FR 2897069 B1 FR2897069 B1 FR 2897069B1 FR 0601117 A FR0601117 A FR 0601117A FR 0601117 A FR0601117 A FR 0601117A FR 2897069 B1 FR2897069 B1 FR 2897069B1
Authority
FR
France
Prior art keywords
saccharase
dextrane
dsr
enzyme
construction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR0601117A
Other languages
English (en)
Other versions
FR2897069A1 (fr
Inventor
Pierre Monsan
Simeon Magali Remaud
Veronese Gabrielle Potocki
Claire Moulis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Original Assignee
Centre National de la Recherche Scientifique CNRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR0601117A priority Critical patent/FR2897069B1/fr
Application filed by Centre National de la Recherche Scientifique CNRS filed Critical Centre National de la Recherche Scientifique CNRS
Priority to CA2947729A priority patent/CA2947729A1/fr
Priority to PT111570339T priority patent/PT2365084T/pt
Priority to ES11157033T priority patent/ES2792474T3/es
Priority to EP11157033.9A priority patent/EP2365084B1/fr
Priority to KR1020087021616A priority patent/KR101465805B1/ko
Priority to PT161629134T priority patent/PT3103874T/pt
Priority to BRPI0706981-2A priority patent/BRPI0706981B1/pt
Priority to US12/223,744 priority patent/US7897373B2/en
Priority to CA2639150A priority patent/CA2639150C/fr
Priority to ES16162913T priority patent/ES2800724T3/es
Priority to EP07734270.7A priority patent/EP1981914B1/fr
Priority to MX2008010147A priority patent/MX2008010147A/es
Priority to ES07734270.7T priority patent/ES2585203T3/es
Priority to EP16162913.4A priority patent/EP3103874B1/fr
Priority to PCT/IB2007/000951 priority patent/WO2007091178A2/fr
Priority to JP2008553855A priority patent/JP5753340B2/ja
Priority to AU2007213436A priority patent/AU2007213436B2/en
Publication of FR2897069A1 publication Critical patent/FR2897069A1/fr
Priority to US12/930,748 priority patent/US8263380B2/en
Publication of FR2897069B1 publication Critical patent/FR2897069B1/fr
Application granted granted Critical
Priority to US13/564,776 priority patent/US9109207B2/en
Priority to US14/798,518 priority patent/US9399765B2/en
Priority to US15/190,282 priority patent/US10017748B2/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
    • C08B37/0021—Dextran, i.e. (alpha-1,4)-D-glucan; Derivatives thereof, e.g. Sephadex, i.e. crosslinked dextran
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C12P19/08—Dextran
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01005—Dextransucrase (2.4.1.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
FR0601117A 2006-02-08 2006-02-08 Construction de nouveaux variants de l'enzyme dextrane-saccharase dsr-s par ingienerie moleculaire. Expired - Fee Related FR2897069B1 (fr)

Priority Applications (22)

Application Number Priority Date Filing Date Title
FR0601117A FR2897069B1 (fr) 2006-02-08 2006-02-08 Construction de nouveaux variants de l'enzyme dextrane-saccharase dsr-s par ingienerie moleculaire.
EP16162913.4A EP3103874B1 (fr) 2006-02-08 2007-02-08 Construction de nouvelles variantes de la dextransucrase dsr-s par genie genetique
PT111570339T PT2365084T (pt) 2006-02-08 2007-02-08 Construção de novas variantes de dextrano-sacarase dsr-s através de engenharia genética
PCT/IB2007/000951 WO2007091178A2 (fr) 2006-02-08 2007-02-08 Construction de nouveaux variants de dextransucrase dsr-s par génie génétique
KR1020087021616A KR101465805B1 (ko) 2006-02-08 2007-02-08 유전자 조작법에 의한 덱스트란수크라제 ｄｓｒ－ｓ의 신규변이체의 제조
PT161629134T PT3103874T (pt) 2006-02-08 2007-02-08 Construção de novas variantes de dextrano-sacarase dsr-s através de engenharia genética
BRPI0706981-2A BRPI0706981B1 (pt) 2006-02-08 2007-02-08 Sequência de nucleotídeos, vetor, dextrana sacarase truncada e/ou mutada, proteína de fusão, método de preparação de dextrana sacarase mutada e/ou truncada, método de produção de dextranas e/ou isomalto-oligossacarídeos e uso de uma dextrana sacarase truncada e/ou mutada
US12/223,744 US7897373B2 (en) 2006-02-08 2007-02-08 Construction of new variants of dextransucrase DSR-S by genetic engineering
CA2639150A CA2639150C (fr) 2006-02-08 2007-02-08 Construction de nouveaux variants de dextransucrase dsr-s par genie genetique
ES16162913T ES2800724T3 (es) 2006-02-08 2007-02-08 Construcción de nuevas variantes de dextransacarasa DSR-S por ingeniería genética
EP07734270.7A EP1981914B1 (fr) 2006-02-08 2007-02-08 Construction de nouveaux variants de dextransucrase dsr-s par genie genetique
MX2008010147A MX2008010147A (es) 2006-02-08 2007-02-08 Construccion de nuevas variantes de dextransucrasa dsr-s por ingenieria genetica.
CA2947729A CA2947729A1 (fr) 2006-02-08 2007-02-08 Construction de nouveaux variants de dextransucrase dsr-s par genie genetique
ES11157033T ES2792474T3 (es) 2006-02-08 2007-02-08 Construcción de nuevas variantes de dextransacarasa DSR-S por ingeniería genética
EP11157033.9A EP2365084B1 (fr) 2006-02-08 2007-02-08 Construction de nouvelles variantes de la dextransucrase DSR-S par génie génétique
JP2008553855A JP5753340B2 (ja) 2006-02-08 2007-02-08 遺伝子操作によるデキストランスクラーゼｄｓｒ−ｓの新規変異体の構築
AU2007213436A AU2007213436B2 (en) 2006-02-08 2007-02-08 Construction of new variants of dextransucrase DSR-S by genetic engineering
ES07734270.7T ES2585203T3 (es) 2006-02-08 2007-02-08 Construcción de variantes nuevas de dextransacarasa DSR-S mediante ingeniería genética
US12/930,748 US8263380B2 (en) 2006-02-08 2011-01-14 Construction of new variants of dextransucrase DSR-S by genetic engineering
US13/564,776 US9109207B2 (en) 2006-02-08 2012-08-02 Construction of new variants of dextransucrase DSR-S by genetic engineering
US14/798,518 US9399765B2 (en) 2006-02-08 2015-07-14 Construction of new variants of dextransucrase DSR-S by genetic engineering
US15/190,282 US10017748B2 (en) 2006-02-08 2016-06-23 Construction of new variants of dextransucrase DSR-S by genetic engineering

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0601117A FR2897069B1 (fr) 2006-02-08 2006-02-08 Construction de nouveaux variants de l'enzyme dextrane-saccharase dsr-s par ingienerie moleculaire.

Publications (2)

Publication Number Publication Date
FR2897069A1 FR2897069A1 (fr) 2007-08-10
FR2897069B1 true FR2897069B1 (fr) 2012-06-08

Family

ID=37101659

Family Applications (1)

Application Number Title Priority Date Filing Date
FR0601117A Expired - Fee Related FR2897069B1 (fr) 2006-02-08 2006-02-08 Construction de nouveaux variants de l'enzyme dextrane-saccharase dsr-s par ingienerie moleculaire.

Country Status (12)

Country Link
US (5) US7897373B2 (fr)
EP (3) EP1981914B1 (fr)
JP (1) JP5753340B2 (fr)
KR (1) KR101465805B1 (fr)
AU (1) AU2007213436B2 (fr)
BR (1) BRPI0706981B1 (fr)
CA (2) CA2639150C (fr)
ES (3) ES2800724T3 (fr)
FR (1) FR2897069B1 (fr)
MX (1) MX2008010147A (fr)
PT (2) PT3103874T (fr)
WO (1) WO2007091178A2 (fr)

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JP6077303B2 (ja) 2009-05-07 2017-02-08 タト エ リル アングルディアント フランス ソシエテ パ アクシオンス シンプリフィエ アルファ−（１，２）−分岐アルファ−（１，６）オリゴデキストランを含有する組成物及びアルファ−（１，２）−分岐アルファ−（１，６）オリゴデキストランの製造方法
WO2012031186A1 (fr) 2010-09-03 2012-03-08 Wisconsin Pharmacal Company, Llc. Ovules prébiotiques
WO2015183721A1 (fr) 2014-05-29 2015-12-03 E. I. Du Pont De Nemours And Company Synthèse enzymatique d'une fibre de glucane soluble
WO2015183729A1 (fr) 2014-05-29 2015-12-03 E. I. Du Pont De Nemours And Company Synthèse enzymatique de fibres de glucane soluble
US20170198322A1 (en) 2014-05-29 2017-07-13 E. I. Du Pont De Nemours And Company Enzymatic synthesis of soluble glucan fiber
MX2016015607A (es) 2014-05-29 2017-03-10 Du Pont Síntesis enzimática de fibra soluble de glucano.
CN106414506A (zh) 2014-05-29 2017-02-15 纳幕尔杜邦公司 可溶性葡聚糖纤维的酶促合成
BR112016027887A2 (pt) 2014-05-29 2017-10-24 Du Pont composições de fibra e de carboidrato, produto alimentício, métodos para produzir uma composição de fibra, para produzir uma composição de carboidratos misturada, método para reduzir o índice glicêmico, método para inibir a elevação do nível de açúcar no sangue, composição cosmética, uso da composição e composição
FR3024158A1 (fr) 2014-07-28 2016-01-29 Inst Nat Sciences Appliq Proteine a activite dextrane-saccharase et applications
US10822574B2 (en) 2015-11-13 2020-11-03 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
US10876074B2 (en) 2015-11-13 2020-12-29 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
US10844324B2 (en) 2015-11-13 2020-11-24 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
MX2018006273A (es) 2015-11-26 2018-08-16 Du Pont Polipeptidos capaces de producir glucanos que tienen ramificaciones alfa-1,2 y uso de los mismos.
FR3045608A1 (fr) * 2015-12-18 2017-06-23 Rhodia Operations Dextrane carboxyle
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WO2019055377A1 (fr) 2017-09-13 2019-03-21 E. I. Du Pont De Nemours And Company Glucosyltransférases modifiées
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CN113201513B (zh) * 2021-06-22 2023-06-09 广西产研院生物制造技术研究所有限公司 一种耐热右旋糖酐蔗糖酶突变体及其制备方法与应用

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Also Published As

Publication number Publication date
ES2800724T3 (es) 2021-01-04
AU2007213436A1 (en) 2007-08-16
EP2365084A3 (fr) 2012-08-15
CA2639150A1 (fr) 2007-08-16
EP2365084B1 (fr) 2020-04-22
US20160298091A1 (en) 2016-10-13
ES2792474T3 (es) 2020-11-11
ES2585203T3 (es) 2016-10-04
EP3103874A1 (fr) 2016-12-14
FR2897069A1 (fr) 2007-08-10
US20120289689A1 (en) 2012-11-15
EP1981914A2 (fr) 2008-10-22
JP2009525745A (ja) 2009-07-16
WO2007091178A3 (fr) 2008-01-10
KR101465805B1 (ko) 2014-11-26
PT3103874T (pt) 2020-07-14
US20110178289A1 (en) 2011-07-21
WO2007091178A2 (fr) 2007-08-16
BRPI0706981B1 (pt) 2021-11-23
BRPI0706981A2 (pt) 2011-04-12
CA2639150C (fr) 2016-12-20
US9109207B2 (en) 2015-08-18
PT2365084T (pt) 2020-06-17
US9399765B2 (en) 2016-07-26
CA2947729A1 (fr) 2007-08-16
US7897373B2 (en) 2011-03-01
US20090258389A1 (en) 2009-10-15
EP3103874B1 (fr) 2020-05-06
KR20090010024A (ko) 2009-01-28
US10017748B2 (en) 2018-07-10
EP3103874A9 (fr) 2018-11-07
EP1981914B1 (fr) 2016-05-11
AU2007213436B2 (en) 2012-02-02
US20150344853A1 (en) 2015-12-03
MX2008010147A (es) 2009-06-12
EP2365084A2 (fr) 2011-09-14
US8263380B2 (en) 2012-09-11
JP5753340B2 (ja) 2015-07-22

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