PT2365084T - Construção de novas variantes de dextrano-sacarase dsr-s através de engenharia genética - Google Patents

Construção de novas variantes de dextrano-sacarase dsr-s através de engenharia genética

Info

Publication number
PT2365084T
PT2365084T PT111570339T PT11157033T PT2365084T PT 2365084 T PT2365084 T PT 2365084T PT 111570339 T PT111570339 T PT 111570339T PT 11157033 T PT11157033 T PT 11157033T PT 2365084 T PT2365084 T PT 2365084T
Authority
PT
Portugal
Prior art keywords
construction
genetic engineering
new variants
dextransucrase dsr
dextransucrase
Prior art date
Application number
PT111570339T
Other languages
English (en)
Original Assignee
Centre Nat Rech Scient
Institut Nat Des Sciences Appliquees De Toulouse Insat
Institut National De Rech Pour Lagriculture Lalimentation Et Lenvironnement
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient, Institut Nat Des Sciences Appliquees De Toulouse Insat, Institut National De Rech Pour Lagriculture Lalimentation Et Lenvironnement filed Critical Centre Nat Rech Scient
Publication of PT2365084T publication Critical patent/PT2365084T/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
    • C08B37/0021—Dextran, i.e. (alpha-1,4)-D-glucan; Derivatives thereof, e.g. Sephadex, i.e. crosslinked dextran
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C12P19/08—Dextran
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01005—Dextransucrase (2.4.1.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Materials Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Peptides Or Proteins (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
PT111570339T 2006-02-08 2007-02-08 Construção de novas variantes de dextrano-sacarase dsr-s através de engenharia genética PT2365084T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0601117A FR2897069B1 (fr) 2006-02-08 2006-02-08 Construction de nouveaux variants de l'enzyme dextrane-saccharase dsr-s par ingienerie moleculaire.

Publications (1)

Publication Number Publication Date
PT2365084T true PT2365084T (pt) 2020-06-17

Family

ID=37101659

Family Applications (2)

Application Number Title Priority Date Filing Date
PT111570339T PT2365084T (pt) 2006-02-08 2007-02-08 Construção de novas variantes de dextrano-sacarase dsr-s através de engenharia genética
PT161629134T PT3103874T (pt) 2006-02-08 2007-02-08 Construção de novas variantes de dextrano-sacarase dsr-s através de engenharia genética

Family Applications After (1)

Application Number Title Priority Date Filing Date
PT161629134T PT3103874T (pt) 2006-02-08 2007-02-08 Construção de novas variantes de dextrano-sacarase dsr-s através de engenharia genética

Country Status (12)

Country Link
US (5) US7897373B2 (pt)
EP (3) EP2365084B1 (pt)
JP (1) JP5753340B2 (pt)
KR (1) KR101465805B1 (pt)
AU (1) AU2007213436B2 (pt)
BR (1) BRPI0706981B1 (pt)
CA (2) CA2947729A1 (pt)
ES (3) ES2585203T3 (pt)
FR (1) FR2897069B1 (pt)
MX (1) MX2008010147A (pt)
PT (2) PT2365084T (pt)
WO (1) WO2007091178A2 (pt)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2897069B1 (fr) * 2006-02-08 2012-06-08 Centre Nat Rech Scient Construction de nouveaux variants de l'enzyme dextrane-saccharase dsr-s par ingienerie moleculaire.
KR101783383B1 (ko) 2009-05-07 2017-09-29 테이트 ＆ 라일 인그레디언츠 프랑스 에스에이에스 알파-(1,2)-분지형 알파-(1,6) 올리고덱스트란을 제조하기 위한 조성물 및 방법
WO2012031186A1 (en) 2010-09-03 2012-03-08 Wisconsin Pharmacal Company, Llc. Prebiotic suppositories
CN106661599A (zh) 2014-05-29 2017-05-10 纳幕尔杜邦公司 可溶性葡聚糖纤维的酶促合成
CN107580453A (zh) 2014-05-29 2018-01-12 纳幕尔杜邦公司 可溶性葡聚糖纤维的酶促合成
WO2015183714A1 (en) * 2014-05-29 2015-12-03 E. I. Du Pont De Nemours And Company Enzymatic synthesis of soluble glucan fiber
EP3149186A1 (en) 2014-05-29 2017-04-05 E. I. du Pont de Nemours and Company Enzymatic synthesis of soluble glucan fiber
EP3149184A1 (en) 2014-05-29 2017-04-05 E. I. du Pont de Nemours and Company Enzymatic synthesis of soluble glucan fiber
WO2015183729A1 (en) 2014-05-29 2015-12-03 E. I. Du Pont De Nemours And Company Enzymatic synthesis of soluble glucan fiber
FR3024158A1 (fr) 2014-07-28 2016-01-29 Inst Nat Sciences Appliq Proteine a activite dextrane-saccharase et applications
US10844324B2 (en) 2015-11-13 2020-11-24 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
EP3374488B1 (en) 2015-11-13 2020-10-14 DuPont Industrial Biosciences USA, LLC Glucan fiber compositions for use in laundry care and fabric care
WO2017083229A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
BR112018010683B8 (pt) 2015-11-26 2022-08-23 Du Pont Método para produzir uma composição de glucano
FR3045608A1 (fr) * 2015-12-18 2017-06-23 Rhodia Operations Dextrane carboxyle
BR112019004213A2 (pt) 2016-09-14 2019-06-25 Du Pont glucosiltransferase não nativa, polinucleotídeo, composição reacional, método de produção de alfa-glucano e método de preparação de uma sequência polinucleotídica que codifica uma glucosiltransferase não nativa
CN106504270B (zh) * 2016-11-08 2019-12-20 浙江大华技术股份有限公司 一种视频中目标物体的展示方法及装置
US10774315B2 (en) 2017-09-13 2020-09-15 Dupont Industrial Biosciences Usa, Llc Engineered glucosyltransferases
US10865393B2 (en) 2017-09-13 2020-12-15 Dupont Industrial Biosciences Usa, Llc Engineered glucosyltransferases
AU2019230061A1 (en) 2018-03-09 2020-09-24 Nutrition & Biosciences USA 4, Inc. Engineered glucosyltransferases
CN113201513B (zh) * 2021-06-22 2023-06-09 广西产研院生物制造技术研究所有限公司 一种耐热右旋糖酐蔗糖酶突变体及其制备方法与应用

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2437518A (en) 1943-07-17 1948-03-09 Pharmacia Ab Manufacture of infusion and injection fluids
US3044940A (en) * 1957-04-30 1962-07-17 Bernburg Serum Werk Veb Process for enzymatic synthesis of dextran
FR2601385B1 (fr) * 1986-07-09 1989-09-29 Sucre Rech & Dev Procede de preparation a partir de saccharose d'un melange de sucres a haute teneur en isomaltose par voie enzymatique et produits obtenus
JPS63240757A (ja) * 1987-03-30 1988-10-06 Natl Food Res Inst 新規な食品素材
JPH0198601A (ja) 1987-10-13 1989-04-17 Gunei Kagaku Kogyo Kk 高純度イソマルトオリゴ糖の製造方法
IL90713A0 (en) * 1988-06-21 1990-01-18 Calgene Inc Methods and compositions for altering physical characteristics of fruit and fruit products
JP2779963B2 (ja) 1989-02-09 1998-07-23 株式会社林原生物化学研究所 ビフィズス菌増殖促進剤
US5646016A (en) * 1991-02-06 1997-07-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
US5229277A (en) 1991-03-05 1993-07-20 Louisiana State University Board Of Supervisors Process for the production of dextran polymers of controlled molecular size and molecular size distributions
JPH07274991A (ja) * 1994-04-04 1995-10-24 Fujisawa Pharmaceut Co Ltd デキストランの製造法
CA2409746C (en) 2000-05-18 2014-11-25 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Immunotoxin fusion proteins and means for expression thereof
EP1201131A1 (en) * 2000-10-23 2002-05-02 Société des Produits Nestlé S.A. Method for preparing fermented food products
US6821958B1 (en) 2001-03-06 2004-11-23 Roger K. Hershline Antiviral composition
JP3709435B2 (ja) * 2001-10-03 2005-10-26 独立行政法人食品総合研究所 改変デキストランスクラーゼ、その遺伝子組み換え体、グルカンの製造法
DE10225380B4 (de) * 2002-06-07 2006-07-06 Technische Universität Braunschweig Produktion und Sekretion von Glucosyltransferasen
JP4553604B2 (ja) * 2003-06-30 2010-09-29 明治製菓株式会社 一般食品、保健機能食品または健康補助食品の機能増強組成物及びその方法
US7524645B2 (en) * 2004-12-14 2009-04-28 Centre National De La Recherche Scientifique (Cnrs) Fully active alternansucrases partially deleted in its carboxy-terminal and amino-terminal domains and mutants thereof
EP1672075A1 (en) * 2004-12-17 2006-06-21 Bayer CropScience GmbH Transformed plant expressing a dextransucrase and synthesizing a modified starch
JP2005185290A (ja) 2005-02-28 2005-07-14 Academia Sinica 組換カンジダルゴサリパーゼ
FR2897069B1 (fr) * 2006-02-08 2012-06-08 Centre Nat Rech Scient Construction de nouveaux variants de l'enzyme dextrane-saccharase dsr-s par ingienerie moleculaire.

Also Published As

Publication number Publication date
ES2800724T3 (es) 2021-01-04
PT3103874T (pt) 2020-07-14
WO2007091178A3 (en) 2008-01-10
EP2365084B1 (en) 2020-04-22
FR2897069B1 (fr) 2012-06-08
US10017748B2 (en) 2018-07-10
BRPI0706981A2 (pt) 2011-04-12
JP5753340B2 (ja) 2015-07-22
US20090258389A1 (en) 2009-10-15
EP3103874B1 (en) 2020-05-06
AU2007213436A1 (en) 2007-08-16
FR2897069A1 (fr) 2007-08-10
US9399765B2 (en) 2016-07-26
EP1981914A2 (en) 2008-10-22
ES2585203T3 (es) 2016-10-04
WO2007091178A2 (en) 2007-08-16
US8263380B2 (en) 2012-09-11
US20110178289A1 (en) 2011-07-21
JP2009525745A (ja) 2009-07-16
BRPI0706981B1 (pt) 2021-11-23
KR101465805B1 (ko) 2014-11-26
EP3103874A9 (en) 2018-11-07
US20120289689A1 (en) 2012-11-15
CA2947729A1 (en) 2007-08-16
US20160298091A1 (en) 2016-10-13
EP2365084A3 (en) 2012-08-15
EP2365084A2 (en) 2011-09-14
AU2007213436B2 (en) 2012-02-02
KR20090010024A (ko) 2009-01-28
MX2008010147A (es) 2009-06-12
CA2639150A1 (en) 2007-08-16
ES2792474T3 (es) 2020-11-11
US20150344853A1 (en) 2015-12-03
EP3103874A1 (en) 2016-12-14
CA2639150C (en) 2016-12-20
US7897373B2 (en) 2011-03-01
EP1981914B1 (en) 2016-05-11
US9109207B2 (en) 2015-08-18

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