BR112019004213A2 - glucosiltransferase não nativa, polinucleotídeo, composição reacional, método de produção de alfa-glucano e método de preparação de uma sequência polinucleotídica que codifica uma glucosiltransferase não nativa - Google Patents

glucosiltransferase não nativa, polinucleotídeo, composição reacional, método de produção de alfa-glucano e método de preparação de uma sequência polinucleotídica que codifica uma glucosiltransferase não nativa

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Publication number
BR112019004213A2
BR112019004213A2 BR112019004213A BR112019004213A BR112019004213A2 BR 112019004213 A2 BR112019004213 A2 BR 112019004213A2 BR 112019004213 A BR112019004213 A BR 112019004213A BR 112019004213 A BR112019004213 A BR 112019004213A BR 112019004213 A2 BR112019004213 A2 BR 112019004213A2
Authority
BR
Brazil
Prior art keywords
native glucosyltransferase
polynucleotide
alpha
preparing
sequence encoding
Prior art date
Application number
BR112019004213A
Other languages
English (en)
Portuguese (pt)
Inventor
D Semke Ellen
B Parker Jared
S Payne Mark
Cheng Qiong
R Bott Richard
Dicosimo Robert
Kralj Slavko
Marie Hennessey Susan
Alkan Veli
Li Yougen
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR112019004213A2 publication Critical patent/BR112019004213A2/pt

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08BPOLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0009Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/18Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Polymers & Plastics (AREA)
  • Materials Engineering (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
BR112019004213A 2016-09-14 2017-09-13 glucosiltransferase não nativa, polinucleotídeo, composição reacional, método de produção de alfa-glucano e método de preparação de uma sequência polinucleotídica que codifica uma glucosiltransferase não nativa BR112019004213A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662394354P 2016-09-14 2016-09-14
PCT/US2017/051279 WO2018052942A1 (en) 2016-09-14 2017-09-13 Engineered glucosyltransferases

Publications (1)

Publication Number Publication Date
BR112019004213A2 true BR112019004213A2 (pt) 2019-06-25

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Family Applications (1)

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BR112019004213A BR112019004213A2 (pt) 2016-09-14 2017-09-13 glucosiltransferase não nativa, polinucleotídeo, composição reacional, método de produção de alfa-glucano e método de preparação de uma sequência polinucleotídica que codifica uma glucosiltransferase não nativa

Country Status (9)

Country Link
US (4) US10301604B2 (https=)
EP (1) EP3512955B1 (https=)
JP (1) JP7136783B2 (https=)
CN (1) CN109715818A (https=)
AU (1) AU2017326168B2 (https=)
BR (1) BR112019004213A2 (https=)
CA (1) CA3033369A1 (https=)
FI (1) FI3512955T3 (https=)
WO (1) WO2018052942A1 (https=)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3630993A1 (en) 2017-05-23 2020-04-08 DuPont Industrial Biosciences USA, LLC Enzymatic production of alpha-1,3-glucan
US10865393B2 (en) 2017-09-13 2020-12-15 Dupont Industrial Biosciences Usa, Llc Engineered glucosyltransferases
EP3682003A1 (en) 2017-09-13 2020-07-22 DuPont Industrial Biosciences USA, LLC Engineered glucosyltransferases
JP7343490B2 (ja) 2017-10-13 2023-09-12 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー,インコーポレイテッド 流動性バルク粒状多糖
US11098334B2 (en) 2017-12-14 2021-08-24 Nutrition & Biosciences USA 4, Inc. Alpha-1,3-glucan graft copolymers
CN112368374A (zh) * 2018-03-09 2021-02-12 营养与生物科学美国4公司 工程化的葡糖基转移酶
CN112638163A (zh) * 2018-07-05 2021-04-09 杜邦营养生物科学有限公司 葡糖基转移酶在发酵奶基产品中提供改善的质地的用途
EP3870616B1 (en) 2018-10-25 2023-10-11 DuPont Industrial Biosciences USA, LLC Alpha-1,3-glucan graft copolymers
US20220033531A1 (en) 2018-12-17 2022-02-03 Dupont Industrial Biosciences Usa,Llc Polysaccharide Derivatives and Compositions Comprising Same
US20220267745A1 (en) * 2019-07-09 2022-08-25 Nutrition & Biosciences USA 4, Inc. Engineered alpha-1,3 branching enzymes
EP4013241A1 (en) 2019-08-16 2022-06-22 Nutrition & Biosciences USA 4, Inc. Flour- and meal-based food products comprising insoluble alpha-1,3-glucan
JP7849032B2 (ja) 2019-11-06 2026-04-21 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー,インコーポレイテッド 高結晶質アルファ-1,3-グルカン
CN115052905B (zh) 2020-02-04 2024-06-11 营养与生物科学美国4公司 包含α-1,3糖苷键的不溶性α-葡聚糖的水性分散体
JP2023528442A (ja) 2020-06-04 2023-07-04 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー,インコーポレイテッド デキストラン-α-グルカングラフトコポリマー及びその誘導体
WO2021252569A1 (en) 2020-06-10 2021-12-16 Nutrition & Biosciences USA 4, Inc. Poly alpha-1,6-glucan derivatives and compositions comprising same
EP4294848A1 (en) 2021-02-19 2023-12-27 Nutrition & Biosciences USA 4, Inc. Oxidized polysaccharide derivatives
WO2022235735A1 (en) 2021-05-04 2022-11-10 Nutrition & Biosciences USA 4, Inc. Compositions comprising oxidized insoluble alpha-glucan
CN117242102A (zh) 2021-05-04 2023-12-15 营养与生物科学美国4公司 包含不溶性α-葡聚糖的组合物
JP2024525685A (ja) 2021-07-13 2024-07-12 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー,インコーポレイテッド カチオン性グルカンエステル誘導体
EP4408197A1 (en) 2021-09-30 2024-08-07 International N&H Denmark ApS Method for reducing sugar in food stuff
EP4426362A1 (en) 2021-11-05 2024-09-11 Nutrition & Biosciences USA 4, Inc. Glucan derivatives for microbial control
EP4463519A1 (en) 2022-01-12 2024-11-20 Nutrition & Biosciences USA 4, Inc. Coating compositions comprising rubber and insoluble alpha-glucan
EP4496834A1 (en) 2022-03-21 2025-01-29 Nutrition & Biosciences USA 4, Inc. Compositions comprising insoluble alpha-glucan
CN114717213B (zh) * 2022-05-05 2023-09-22 广西科学院 一种葡聚糖蔗糖酶的n端截短突变酶及其制备方法
JP2025523007A (ja) 2022-07-11 2025-07-17 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー,インコーポレイテッド 両親媒性グルカンエステル誘導体
WO2024086560A1 (en) 2022-10-17 2024-04-25 International N&H Denmark Aps Method for improving flavor in plant-based food stuff
US20250339361A1 (en) 2022-11-02 2025-11-06 Nutrition & Biosciences USA 4, Inc. Compositions comprising xanthan gum and crystalline alpha-1,3-glucan
AU2024242918A1 (en) 2023-03-29 2025-09-18 Danisco Us Inc. Methods for modifying texture in foodstuff via preferably in situ produced alpha-glucan
CN121398902A (zh) 2023-06-30 2026-01-23 营养与生物科学美国第四公司 多孔α-1,3-葡聚糖组合物
WO2025072419A1 (en) 2023-09-29 2025-04-03 Nutrition & Biosciences Usa 1, Llc Crosslinked alpha-glucan derivatives
WO2025072417A1 (en) 2023-09-29 2025-04-03 Nutrition & Biosciences USA 4, Inc. Polysaccharide derivatives
WO2025072416A1 (en) 2023-09-29 2025-04-03 Nutrition & Biosciences USA 4, Inc. Polysaccharide derivatives
WO2025198996A1 (en) 2024-03-19 2025-09-25 Danisco Us Inc. Method for improving flavor in foodstuff
WO2026015598A2 (en) 2024-07-11 2026-01-15 International N&H Denmark Aps Method to provide improved texture and stable sucrose levels in foodstuff with glucosyltransferase
WO2026024663A1 (en) 2024-07-24 2026-01-29 Nutrition & Biosciences USA 4, Inc. Compositions comprising alpha-glucan with alpha-1,3 glycosidic linkages
WO2026039397A1 (en) 2024-08-13 2026-02-19 Nutrition & Biosciences USA 4, Inc. Alpha-glucan graft copolymer derivatives

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1001736A1 (en) 1996-10-11 2000-05-24 Novo Nordisk A/S STARCH BINDING DOMAINS (SBDs) FOR ORAL CARE PRODUCTS
US5952205A (en) 1998-02-06 1999-09-14 Neose Technologies, Inc. Process for processing sucrose into glucose and fructose
JP2000175694A (ja) 1998-12-17 2000-06-27 Lion Corp 酵素合成によるムタンの製造方法
DE60009886T2 (de) 1999-01-25 2005-03-31 E.I. Du Pont De Nemours And Co., Wilmington Polysaccharidfasern
US6867026B2 (en) 2000-05-25 2005-03-15 Nederlandse Organisatie Voor Toegepast Natuurwetenschappelijk Onderzoek Tno Glucosyltransferases
JP3709435B2 (ja) * 2001-10-03 2005-10-26 独立行政法人食品総合研究所 改変デキストランスクラーゼ、その遺伝子組み換え体、グルカンの製造法
CA2480962A1 (en) * 2002-03-07 2003-09-18 The Forsyth Institute Immunogenicity of glucan binding protein
US7780970B2 (en) 2002-08-06 2010-08-24 Danisco A/S Composition
US7524645B2 (en) 2004-12-14 2009-04-28 Centre National De La Recherche Scientifique (Cnrs) Fully active alternansucrases partially deleted in its carboxy-terminal and amino-terminal domains and mutants thereof
EP1679374A1 (en) 2005-01-10 2006-07-12 Bayer CropScience GmbH Transformed plant expressing a mutansucrase and synthesizing a modified starch
FR2897069B1 (fr) 2006-02-08 2012-06-08 Centre Nat Rech Scient Construction de nouveaux variants de l'enzyme dextrane-saccharase dsr-s par ingienerie moleculaire.
EP2100966A1 (en) 2008-03-12 2009-09-16 Institut Pasteur Mutants of glycoside hydrolases and uses thereof for synthesizing complex oligosaccharides and disaccharide intermediates
JP5680860B2 (ja) * 2009-01-30 2015-03-04 サントリーホールディングス株式会社 フラボノイド3位ガラクトース・グルコース転移酵素、及びそれをコードするポリヌクレオチド
JP2011206273A (ja) 2010-03-30 2011-10-20 Terumo Corp 注射針組立体および薬剤注射装置
CA2798118A1 (en) 2010-05-04 2011-11-10 Sanofi-Aventis Deutschland Gmbh Tactile identification of drug filled cartridge
JP5015287B2 (ja) 2010-05-11 2012-08-29 シャープ株式会社 加熱調理器
JP5851149B2 (ja) 2011-08-08 2016-02-03 株式会社ニューフレアテクノロジー 成膜装置および成膜方法
US8642757B2 (en) 2011-09-09 2014-02-04 E I Du Pont De Nemours And Company High titer production of highly linear poly (α 1,3 glucan)
US9080195B2 (en) 2011-09-09 2015-07-14 E I Du Pont De Nemours And Company High titer production of poly (α 1,3 glucan)
WO2014052386A2 (en) 2012-09-25 2014-04-03 E. I. Du Pont De Nemours And Company Glucosyltransferase enzymes for production of glucan polymers
CN104968781A (zh) * 2012-12-24 2015-10-07 诺维信公司 具有内切葡聚糖酶活性的多肽以及对其进行编码的多核苷酸
US9169506B2 (en) 2013-09-05 2015-10-27 E I Du Pont De Nemours And Company Process for producing alpha-1,3-glucan polymer with reduced molecular weight
US9644190B2 (en) 2014-02-06 2017-05-09 The United States Of America, As Represented By The Secretary Of Agriculture Modified glucansucrase and related methods
WO2015123327A1 (en) 2014-02-14 2015-08-20 E. I. Du Pont De Nemours And Company Glucosyltransferase enzymes for production of glucan polymers
WO2015123323A1 (en) 2014-02-14 2015-08-20 E. I. Du Pont De Nemours And Company Poly-alpha-1,3-1,6-glucans for viscosity modification
BR112016027867A2 (pt) 2014-05-29 2017-10-24 Du Pont composições de fibra e de carboidrato, produto alimentício, métodos para produzir uma composição de fibra, de carboidrato, para reduzir o índice glicêmico e para inibir a elevação do nível de açúcar, composição cosmética, uso da composição de fibra e composição
MX2016015703A (es) 2014-05-29 2017-03-16 Du Pont Sintesis enzimatica de fibra de glucano soluble.
WO2015183722A1 (en) 2014-05-29 2015-12-03 E. I. Du Pont De Nemours And Company Enzymatic synthesis of soluble glucan fiber
BR112016027887A2 (pt) 2014-05-29 2017-10-24 Du Pont composições de fibra e de carboidrato, produto alimentício, métodos para produzir uma composição de fibra, para produzir uma composição de carboidratos misturada, método para reduzir o índice glicêmico, método para inibir a elevação do nível de açúcar no sangue, composição cosmética, uso da composição e composição
US20170204442A1 (en) 2014-05-29 2017-07-20 E I Du Pont De Nemours And Company Enzymatic synthesis of soluble glucan fiber
CA2949289A1 (en) 2014-05-29 2015-12-03 E. I. Du Pont De Nemours And Company Enzymatic synthesis of soluble glucan fiber
US10059779B2 (en) 2014-11-05 2018-08-28 E I Du Pont De Nemours And Company Enzymatically polymerized gelling dextrans
CA2989337A1 (en) 2015-06-17 2016-12-22 E I Du Pont De Nemours And Company Glucosyltransferase amino acid sequence motifs for producing linear poly alpha-1,3-glucan

Also Published As

Publication number Publication date
US20240417702A1 (en) 2024-12-19
US20190292528A1 (en) 2019-09-26
US10301604B2 (en) 2019-05-28
JP7136783B2 (ja) 2022-09-13
US11028373B2 (en) 2021-06-08
US11952598B2 (en) 2024-04-09
JP2019530474A (ja) 2019-10-24
EP3512955B1 (en) 2023-04-19
CA3033369A1 (en) 2018-03-22
CN109715818A (zh) 2019-05-03
EP3512955A1 (en) 2019-07-24
US12378535B2 (en) 2025-08-05
FI3512955T3 (fi) 2023-07-18
WO2018052942A1 (en) 2018-03-22
AU2017326168B2 (en) 2022-02-24
AU2017326168A1 (en) 2019-02-21
US20180072998A1 (en) 2018-03-15
US20210395705A1 (en) 2021-12-23

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