MX2017002785A - Sintesis de acido desoxirribonucleico (adn). - Google Patents

Sintesis de acido desoxirribonucleico (adn).

Info

Publication number
MX2017002785A
MX2017002785A MX2017002785A MX2017002785A MX2017002785A MX 2017002785 A MX2017002785 A MX 2017002785A MX 2017002785 A MX2017002785 A MX 2017002785A MX 2017002785 A MX2017002785 A MX 2017002785A MX 2017002785 A MX2017002785 A MX 2017002785A
Authority
MX
Mexico
Prior art keywords
dna
synthesis
nucleotides
reaction mixture
relates
Prior art date
Application number
MX2017002785A
Other languages
English (en)
Inventor
Porter Neil
Rothwell Paul
Extance Jonathan
Original Assignee
Touchlight Ip Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Touchlight Ip Ltd filed Critical Touchlight Ip Ltd
Publication of MX2017002785A publication Critical patent/MX2017002785A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/113—Telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/109—Pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/153—Viscosity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a un proceso mejorado para la síntesis de ADN, ARN, proteínas y moléculas similares, en particular, síntesis de DNA enzimática libre de células, preferiblemente a gran escala. La presente invención se refiere a la síntesis de ADN mediante el uso de replicación por desplazamiento de hebras, y la adición de nucleótidos a la mezcla de reacción es controlada, controlando así el rendimiento. La mezcla de reacción contiene una cantidad inicial de nucleótidos, polimerasa y plantilla de ADN, a la cual se suministran nucleótidos adicionales de una manera controlada.
MX2017002785A 2014-09-05 2015-08-21 Sintesis de acido desoxirribonucleico (adn). MX2017002785A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1415789.5A GB201415789D0 (en) 2014-09-05 2014-09-05 Synthesis of DNA
PCT/GB2015/052434 WO2016034849A1 (en) 2014-09-05 2015-08-21 Synthesis of dna

Publications (1)

Publication Number Publication Date
MX2017002785A true MX2017002785A (es) 2017-09-28

Family

ID=51796300

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017002785A MX2017002785A (es) 2014-09-05 2015-08-21 Sintesis de acido desoxirribonucleico (adn).

Country Status (19)

Country Link
US (1) US10501782B1 (es)
EP (1) EP3188836B1 (es)
JP (1) JP6708631B2 (es)
KR (1) KR102497228B1 (es)
CN (1) CN107075577B (es)
AU (1) AU2015310728B2 (es)
BR (1) BR112017003836B1 (es)
CA (1) CA2959974A1 (es)
DK (1) DK3188836T3 (es)
ES (1) ES2973011T3 (es)
FI (1) FI3188836T3 (es)
GB (1) GB201415789D0 (es)
IL (1) IL250612B (es)
LT (1) LT3188836T (es)
MX (1) MX2017002785A (es)
RU (1) RU2714253C2 (es)
SG (1) SG11201701616PA (es)
WO (1) WO2016034849A1 (es)
ZA (1) ZA201701246B (es)

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US10077459B2 (en) 2016-05-04 2018-09-18 General Electric Company Cell-free protein expression using rolling circle amplification product
WO2018033730A1 (en) * 2016-08-16 2018-02-22 Touchlight IP Limited Closed linear dna production
US11001868B2 (en) 2017-08-11 2021-05-11 Global Life Sciences Solutions Operations UK Ltd Cell-free protein expression using double-stranded concatameric DNA
GB201813429D0 (en) * 2018-08-17 2018-10-03 Touchlight Ip Ltd Synthesis of DNA with improved yield
EP3792367A1 (en) 2019-09-11 2021-03-17 Universität Bielefeld Method for the production of raav and method for the in vitro generation of genetically engineered, linear, single-stranded nucleic acid fragments containing itr sequences flanking a gene of interest
GB202002068D0 (en) 2020-02-14 2020-04-01 Touchlight Ip Ltd DNA synthesis yield improvements
GB202007428D0 (en) 2020-05-19 2020-07-01 Fabricnano Ltd Polynucleotide synthesis
US11720801B2 (en) 2020-08-25 2023-08-08 International Business Machines Corporation Chemical reaction network for estimating concentration of chemical species based on an identified pattern of output chemical species
US20220356515A1 (en) * 2021-05-10 2022-11-10 Pacific Biosciences Of California, Inc. Dna amplification buffer replenishment during rolling circle amplification
GB202111742D0 (en) 2021-08-16 2021-09-29 Touchlight Ip Ltd Improved process
GB202114105D0 (en) 2021-10-01 2021-11-17 Fabricnano Ltd Nucleotide synthesis
WO2023122526A1 (en) 2021-12-20 2023-06-29 Zoetis Services Llc Use of interferon as an adjuvant in vaccines
WO2023122525A1 (en) 2021-12-20 2023-06-29 Zoetis Services Llc Compositions and methods for prevention of piscine myocarditis
WO2023122523A1 (en) 2021-12-20 2023-06-29 Zoetis Services Llc Compositions and methods for expressing antigens on cell membrane
EP4293101A1 (en) 2022-06-14 2023-12-20 Asklepios Biopharmaceutical, Inc. Reactor with temperature control and method of using the same
WO2024059486A1 (en) 2022-09-12 2024-03-21 Zoetis Services Llc Method of administration of aquaculture vaccines
WO2024115669A1 (en) * 2022-11-30 2024-06-06 Evaxion Biotech A/S Process for production of synthetic circular dna
US11981936B1 (en) 2023-09-29 2024-05-14 New England Biolabs, Inc. TelA variants, compositions, and methods

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US6953676B1 (en) 1992-05-01 2005-10-11 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
DE69429038T2 (de) 1993-07-28 2002-03-21 Pe Corp Ny Norwalk Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung
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RU2218414C2 (ru) 2001-05-04 2003-12-10 Институт молекулярной биологии им. В.А. Энгельгардта РАН Способ интеграции множественных полимеразных реакций амплификации с последующим анализом амплифицированных последовательностей непосредственно на биочипе
US20030077611A1 (en) 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
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WO2006119066A2 (en) * 2005-04-29 2006-11-09 The J. Craig Venter Institute Amplification and cloning of single dna molecules using rolling circle amplification
EP3527671A1 (en) 2006-10-06 2019-08-21 Applied DNA Sciences Inc. System for a continuous rapid thermal cycle system
GB201013153D0 (en) * 2010-08-04 2010-09-22 Touchlight Genetics Ltd Primer for production of closed linear DNA
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Also Published As

Publication number Publication date
JP2017525384A (ja) 2017-09-07
RU2714253C2 (ru) 2020-02-13
CN107075577A (zh) 2017-08-18
AU2015310728A1 (en) 2017-03-09
EP3188836B1 (en) 2023-12-20
EP3188836A1 (en) 2017-07-12
IL250612A0 (en) 2017-04-30
US10501782B1 (en) 2019-12-10
GB201415789D0 (en) 2014-10-22
DK3188836T3 (da) 2024-03-11
CA2959974A1 (en) 2016-03-10
SG11201701616PA (en) 2017-03-30
KR102497228B1 (ko) 2023-02-07
JP6708631B2 (ja) 2020-06-10
CN107075577B (zh) 2022-01-11
AU2015310728B2 (en) 2021-09-16
BR112017003836A2 (pt) 2018-04-10
WO2016034849A1 (en) 2016-03-10
ZA201701246B (en) 2021-05-26
FI3188836T3 (fi) 2024-02-21
RU2017111097A3 (es) 2018-10-05
KR20170045349A (ko) 2017-04-26
ES2973011T3 (es) 2024-06-18
RU2017111097A (ru) 2018-10-05
BR112017003836B1 (pt) 2022-04-05
LT3188836T (lt) 2024-03-25
IL250612B (en) 2021-01-31

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