JP2019516964A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2019516964A5 JP2019516964A5 JP2018553973A JP2018553973A JP2019516964A5 JP 2019516964 A5 JP2019516964 A5 JP 2019516964A5 JP 2018553973 A JP2018553973 A JP 2018553973A JP 2018553973 A JP2018553973 A JP 2018553973A JP 2019516964 A5 JP2019516964 A5 JP 2019516964A5
- Authority
- JP
- Japan
- Prior art keywords
- hcp
- antibody
- sample
- recombinant polypeptide
- host cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 19
- 229920001184 polypeptide Polymers 0.000 claims 8
- 108090000765 processed proteins & peptides Proteins 0.000 claims 8
- 102000004196 processed proteins & peptides Human genes 0.000 claims 8
- 210000004027 cell Anatomy 0.000 claims 7
- 238000002360 preparation method Methods 0.000 claims 7
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 4
- 239000000758 substrate Substances 0.000 claims 4
- 239000003153 chemical reaction reagent Substances 0.000 claims 3
- 238000004519 manufacturing process Methods 0.000 claims 3
- 241001465754 Metazoa Species 0.000 claims 2
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 2
- 238000012544 monitoring process Methods 0.000 claims 2
- 108090001008 Avidin Proteins 0.000 claims 1
- 241000283707 Capra Species 0.000 claims 1
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 claims 1
- 241001494479 Pecora Species 0.000 claims 1
- 230000000919 anti-host Effects 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 claims 1
- 239000013256 coordination polymer Substances 0.000 claims 1
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 238000011143 downstream manufacturing Methods 0.000 claims 1
- 239000012467 final product Substances 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 230000001225 therapeutic effect Effects 0.000 claims 1
- 238000011144 upstream manufacturing Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662322621P | 2016-04-14 | 2016-04-14 | |
| US62/322,621 | 2016-04-14 | ||
| PCT/EP2017/058775 WO2017178526A1 (en) | 2016-04-14 | 2017-04-12 | Compositions and methods for the detection of host cell proteins |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019516964A JP2019516964A (ja) | 2019-06-20 |
| JP2019516964A5 true JP2019516964A5 (enExample) | 2020-04-23 |
| JP7565147B2 JP7565147B2 (ja) | 2024-10-10 |
Family
ID=58645011
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018553973A Active JP7565147B2 (ja) | 2016-04-14 | 2017-04-12 | 宿主細胞タンパク質の検出のための組成物及び方法 |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US11353468B2 (enExample) |
| EP (2) | EP3443348A1 (enExample) |
| JP (1) | JP7565147B2 (enExample) |
| KR (1) | KR102408260B1 (enExample) |
| CN (2) | CN109564217A (enExample) |
| AU (1) | AU2017251360A1 (enExample) |
| BR (1) | BR112018071107A2 (enExample) |
| CA (1) | CA3020808A1 (enExample) |
| EA (1) | EA201892325A1 (enExample) |
| ES (1) | ES2994760T3 (enExample) |
| IL (2) | IL290358B2 (enExample) |
| MX (1) | MX2018012550A (enExample) |
| SG (1) | SG11201808992UA (enExample) |
| WO (1) | WO2017178526A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109564217A (zh) | 2016-04-14 | 2019-04-02 | 隆扎有限公司 | 用于检测宿主细胞蛋白的组合物和方法 |
| US11268951B2 (en) * | 2017-09-20 | 2022-03-08 | Charles River Laboratories International, Inc. | CHO host cell protein assay |
| CN109078628B (zh) * | 2018-08-27 | 2021-06-11 | 西北大学 | 一种以苄胺为配基的高效疏水相互作用色谱介质、制备方法及其在蛋白质复性与纯化的应用 |
| CN111665364B (zh) * | 2019-03-08 | 2025-01-21 | 湖州申科生物技术股份有限公司 | 一种评估hcp检测用多克隆抗体覆盖率的方法 |
| KR20220107586A (ko) | 2021-01-25 | 2022-08-02 | 금오공과대학교 산학협력단 | 전기신호를 이용한 세포수 검출장치 및 이를 이용한 검출방법 |
| CN114966038A (zh) * | 2022-04-13 | 2022-08-30 | 翌圣生物科技(上海)股份有限公司 | 精确定量检测生物医药制品中残留sp2/0宿主细胞蛋白的方法及ELISA检测试剂盒 |
| WO2024084264A1 (en) * | 2022-10-17 | 2024-04-25 | HAJIAN TEHRANI, Behrooz | Immunoassay for detecting host cell proteins of high five cells |
| CN115894677A (zh) * | 2022-12-01 | 2023-04-04 | 湖州申科生物技术股份有限公司 | 一种提高hcp检测抗体覆盖率的抗体组合及其应用 |
| CN117192107A (zh) * | 2023-09-11 | 2023-12-08 | 福建基诺厚普生物科技有限公司 | 一种工艺特异性宿主细胞蛋白残留的检测方法及试剂盒 |
| US20250334572A1 (en) * | 2024-04-26 | 2025-10-30 | Waters Technologies Corporation | Host cell protein detection using lateral flow devices |
| CN118465164B (zh) * | 2024-05-10 | 2025-02-14 | 上海澳斯康生物制药有限公司 | 一种用于检测hcp抗体覆盖率的方法及其应用 |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2046920B1 (enExample) | 1969-06-19 | 1974-05-03 | Citizen Watch Co Ltd | |
| US3940475A (en) | 1970-06-11 | 1976-02-24 | Biological Developments, Inc. | Radioimmune method of assaying quantitatively for a hapten |
| US4737456A (en) | 1985-05-09 | 1988-04-12 | Syntex (U.S.A.) Inc. | Reducing interference in ligand-receptor binding assays |
| US5272071A (en) | 1989-12-22 | 1993-12-21 | Applied Research Systems Ars Holding N.V. | Method for the modification of the expression characteristics of an endogenous gene of a given cell line |
| GB9022545D0 (en) | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| US6797522B1 (en) * | 1994-01-13 | 2004-09-28 | The Trustees Of Columbia University In The City Of New York | Synthetic receptors |
| EP1958962A3 (en) | 1997-06-12 | 2013-05-01 | Novartis International Pharmaceutical Ltd. | Artificial antibody polypeptides |
| WO1999034220A2 (en) * | 1997-12-31 | 1999-07-08 | Genentech, Inc. | Real-time monitoring of an analyte by chromatography using an on-line assay |
| JP2003516124A (ja) | 1999-10-15 | 2003-05-13 | ユニバーシティー オブ マサチューセッツ | 標的とした遺伝的干渉の手段としてのｒｎａ干渉経路遺伝子 |
| US6326193B1 (en) | 1999-11-05 | 2001-12-04 | Cambria Biosciences, Llc | Insect control agent |
| CN1321753A (zh) * | 2000-04-29 | 2001-11-14 | 上海博德基因开发有限公司 | 一种新的多肽——人b细胞蛋白酪氨酸激酶13和编码这种多肽的多核苷酸 |
| WO2001096584A2 (en) | 2000-06-12 | 2001-12-20 | Akkadix Corporation | Materials and methods for the control of nematodes |
| US20030091976A1 (en) * | 2001-11-14 | 2003-05-15 | Ciphergen Biosystems, Inc. | Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry |
| WO2004094989A2 (en) * | 2003-04-22 | 2004-11-04 | Ciphergen Biosystems, Inc. | Methods of host cell protein analysis |
| WO2005071410A1 (en) | 2004-01-13 | 2005-08-04 | Tanox, Inc. | Detecting and quantifying host cell proteins in recombinant protein products |
| US8728828B2 (en) * | 2004-12-22 | 2014-05-20 | Ge Healthcare Bio-Sciences Ab | Purification of immunoglobulins |
| CN101571549B (zh) * | 2008-04-30 | 2013-08-14 | 上海泽润生物科技有限公司 | Vero细胞HCP检测试剂盒及其应用 |
| CN105111309A (zh) * | 2008-10-20 | 2015-12-02 | Abbvie公司 | 使用a蛋白亲和层析分离和纯化抗体 |
| BR112015009553A2 (pt) | 2012-10-30 | 2017-10-03 | Hoffmann La Roche | Método para separar uma proteína de interesse de uma solução de cultura de células |
| CN103439512B (zh) * | 2013-07-30 | 2014-05-07 | 通化东宝药业股份有限公司 | 一种检测特异性重组人胰岛素大肠杆菌残留宿主蛋白的方法 |
| KR102571391B1 (ko) * | 2013-09-13 | 2023-08-29 | 제넨테크, 인크. | 정제된 재조합 폴리펩티드를 포함하는 방법 및 조성물 |
| CN103792366B (zh) * | 2014-01-21 | 2015-09-23 | 内蒙古必威安泰生物科技有限公司 | 口蹄疫疫苗宿主细胞蛋白双抗夹心酶联免疫检测试剂盒及使用方法 |
| CN105021824A (zh) * | 2015-07-06 | 2015-11-04 | 上海优者生物科技有限公司 | 用于定量检测生物医药制品中残留cho宿主细胞蛋白的elisa试剂盒及其使用方法 |
| CN109564217A (zh) | 2016-04-14 | 2019-04-02 | 隆扎有限公司 | 用于检测宿主细胞蛋白的组合物和方法 |
-
2017
- 2017-04-12 CN CN201780034661.8A patent/CN109564217A/zh active Pending
- 2017-04-12 AU AU2017251360A patent/AU2017251360A1/en not_active Abandoned
- 2017-04-12 KR KR1020187032543A patent/KR102408260B1/ko active Active
- 2017-04-12 BR BR112018071107-1A patent/BR112018071107A2/pt not_active Application Discontinuation
- 2017-04-12 JP JP2018553973A patent/JP7565147B2/ja active Active
- 2017-04-12 IL IL290358A patent/IL290358B2/en unknown
- 2017-04-12 US US16/093,253 patent/US11353468B2/en active Active
- 2017-04-12 SG SG11201808992UA patent/SG11201808992UA/en unknown
- 2017-04-12 EP EP17720378.3A patent/EP3443348A1/en not_active Withdrawn
- 2017-04-12 ES ES20160929T patent/ES2994760T3/es active Active
- 2017-04-12 EA EA201892325A patent/EA201892325A1/ru unknown
- 2017-04-12 MX MX2018012550A patent/MX2018012550A/es unknown
- 2017-04-12 CN CN202210618930.8A patent/CN115109148A/zh active Pending
- 2017-04-12 WO PCT/EP2017/058775 patent/WO2017178526A1/en not_active Ceased
- 2017-04-12 CA CA3020808A patent/CA3020808A1/en not_active Abandoned
- 2017-04-12 EP EP20160929.4A patent/EP3683579B1/en active Active
-
2018
- 2018-10-11 IL IL262297A patent/IL262297A/en unknown
-
2022
- 2022-05-03 US US17/735,555 patent/US20220268792A1/en active Pending
- 2022-05-03 US US17/735,524 patent/US12203948B2/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2019516964A5 (enExample) | ||
| US20250271424A1 (en) | Target interference suppressed anti-drug antibody assay | |
| JP6927472B2 (ja) | トロンビン・アンチトロンビン複合体の測定試薬及び測定方法 | |
| US10281469B2 (en) | Mycoplasma pneumoniae detection reagent and application of same | |
| CN101865924A (zh) | 基于免疫磁珠联合酶联免疫吸附试验检测伏马毒素的方法 | |
| CN110873791A (zh) | 一种基于双标记信号放大的间接背景荧光胶体金免疫层析试纸条及其应用 | |
| WO2018047793A1 (ja) | 腫瘍マーカーの測定方法及び測定試薬 | |
| JP5541748B2 (ja) | アビジン−ビオチン連結標識試薬を用いたイムノクロマトグラフ用試験具及びその利用 | |
| JP7315966B2 (ja) | 生物学的試料中の遊離ａｉｍの免疫学的分析方法 | |
| CN106198962B (zh) | 用于封闭生物磁珠的方法 | |
| EP3929584A1 (en) | Method for measuring thyroglobulin | |
| CN106153935B (zh) | 一种定量检测CD79α的酶联免疫试剂盒 | |
| CN112964874B (zh) | 基于间接法的SARS-CoV-2检测试剂盒 | |
| Chen et al. | Simply mixing poly protein G with detection antibodies enhances the detection limit and sensitivity of immunoassays | |
| CN112964872A (zh) | SARS-CoV-2检测试剂盒 | |
| JP7266252B2 (ja) | 新規の免疫グロブリンｅのエピトープ、それと結合する抗体および前記抗体を含む試料のうち免疫グロブリンｅを分析するためのキット | |
| JP2009529133A (ja) | 細胞膜受容体に結合した抗体を検出するための新規なアッセイ方法 | |
| CN117030997B (zh) | 一种小分子化合物的均相免疫分析方法 | |
| Singh et al. | Surface plasmon resonance sensogram based characterization of antibodies raised against intact teliospores and purified diagnostic antigen for development of nano-immunosensor for fungal spore antigen of karnal bunt (Tilletia indica) of wheat | |
| Boas et al. | Method to conjugate polysaccharide antigens to surfaces for the detection of antibodies | |
| Fu et al. | A modified quick PETIA for detecting anti-CCP antibodies in human serum | |
| JP5701666B2 (ja) | 高親和性抗ハプテンポリクローナル抗体の作製法 | |
| Li et al. | Establishment and Evaluation of Immunocolloidal Gold Chromatography Method in the Sandwich Mode for Novel Coronavirus Double-Antibody Detection | |
| CN105137083A (zh) | 一种针对HPA-1a/3a/5b抗体的MAIPA检测试剂盒及检测方法 | |
| JP2024093998A (ja) | 非特異反応を抑制可能な抗体溶液 |