JP2019510482A5 - - Google Patents

Info

Publication number

JP2019510482A5

JP2019510482A5 JP2018544785A JP2018544785A JP2019510482A5 JP 2019510482 A5 JP2019510482 A5 JP 2019510482A5 JP 2018544785 A JP2018544785 A JP 2018544785A JP 2018544785 A JP2018544785 A JP 2018544785A JP 2019510482 A5 JP2019510482 A5 JP 2019510482A5

Authority

JP

Japan

Prior art keywords

cells

methods

derived

culture medium

beta cells

Prior art date

2017-02-24

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 238000000034 method Methods 0.000 claims 13
• 239000003795 chemical substances by application Substances 0.000 claims 7
• 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 claims 6
• 210000004027 cell Anatomy 0.000 claims 4
• 239000003112 inhibitor Substances 0.000 claims 4
• 239000002243 precursor Substances 0.000 claims 4
• 230000019491 signal transduction Effects 0.000 claims 4
• 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 claims 3
• 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 claims 3
• 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 claims 3
• 239000006143 cell culture medium Substances 0.000 claims 3
• 230000002124 endocrine Effects 0.000 claims 3
• 210000001778 pluripotent stem cell Anatomy 0.000 claims 3
• DWJXYEABWRJFSP-XOBRGWDASA-N DAPT Chemical group N([C@@H](C)C(=O)N[C@H](C(=O)OC(C)(C)C)C=1C=CC=CC=1)C(=O)CC1=CC(F)=CC(F)=C1 DWJXYEABWRJFSP-XOBRGWDASA-N 0.000 claims 2
• 101000882127 Homo sapiens Histone-lysine N-methyltransferase EZH2 Proteins 0.000 claims 2
• AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical class IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 claims 2
• 239000012190 activator Substances 0.000 claims 2
• CJGYSWNGNKCJSB-YVLZZHOMSA-N bucladesine Chemical group C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](OC(=O)CCC)[C@@H]2N1C(N=CN=C2NC(=O)CCC)=C2N=C1 CJGYSWNGNKCJSB-YVLZZHOMSA-N 0.000 claims 2
• 229960005263 bucladesine Drugs 0.000 claims 2
• 238000011977 dual antiplatelet therapy Methods 0.000 claims 2
• 239000003540 gamma secretase inhibitor Substances 0.000 claims 2
• 210000000130 stem cell Anatomy 0.000 claims 2
• ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 claims 2
• 239000005495 thyroid hormone Substances 0.000 claims 2
• 229940036555 thyroid hormone Drugs 0.000 claims 2
• OMKHWTRUYNAGFG-IEBDPFPHSA-N 3-deazaneplanocin a Chemical group C1=NC=2C(N)=NC=CC=2N1[C@@H]1C=C(CO)[C@@H](O)[C@H]1O OMKHWTRUYNAGFG-IEBDPFPHSA-N 0.000 claims 1
• 229940125373 Gamma-Secretase Inhibitor Drugs 0.000 claims 1
• HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims 1
• 102000004887 Transforming Growth Factor beta Human genes 0.000 claims 1
• 108090001012 Transforming Growth Factor beta Proteins 0.000 claims 1
• DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 claims 1
• 239000007640 basal medium Substances 0.000 claims 1
• 238000012258 culturing Methods 0.000 claims 1
• 229940079593 drug Drugs 0.000 claims 1
• 239000003814 drug Substances 0.000 claims 1
• 230000003028 elevating effect Effects 0.000 claims 1
• 229960003692 gamma aminobutyric acid Drugs 0.000 claims 1
• BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 claims 1
• 239000001963 growth medium Substances 0.000 claims 1
• 229960002897 heparin Drugs 0.000 claims 1
• 229920000669 heparin Polymers 0.000 claims 1
• 238000004519 manufacturing process Methods 0.000 claims 1
• 239000002609 medium Substances 0.000 claims 1