RU2017125728A - Культивирование плюрипотентных стволовых клеток в суспензии - Google Patents
Культивирование плюрипотентных стволовых клеток в суспензии Download PDFInfo
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- RU2017125728A RU2017125728A RU2017125728A RU2017125728A RU2017125728A RU 2017125728 A RU2017125728 A RU 2017125728A RU 2017125728 A RU2017125728 A RU 2017125728A RU 2017125728 A RU2017125728 A RU 2017125728A RU 2017125728 A RU2017125728 A RU 2017125728A
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- cells
- expression
- endoderm
- ptf1a
- differentiating
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- C12N2501/30—Hormones
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- C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (7)
1. Способ дифференцировки человеческих плюрипотентных клеток, включающий стадии: дифференцировки клеток энтодермы передней кишки в клетки панкреатической энтодермы посредством культивирования клеток энтодермы передней кишки в динамической суспензионной культуре при рН от около 7,2 до около 7,0 в течение, по меньшей мере, около 24 часов.
2. Способ по п. 1, дополнительно включающий культивирование клеток энтодермы передней кишки в культуре, в которой концентрация клеток равна или превышает около 1,5 миллиона клеток/мл.
3. Способ по п. 1, дополнительно включающий культивирование клеток энтодермы передней кишки в культуре, в которой концентрация клеток превышает или равна около 2,0 миллиона клеток/мл.
4. Способ по п. 1, в котором клетки панкреатической энтодермы являются по существу отрицательными в отношении экспрессии PTF1A и NGN3.
5. Способ по п. 4, дополнительно включающий обогащение клеток панкреатической энтодермы, которые являются по существу отрицательными в отношении экспрессии PTF1A и NGN3, до популяции клеток панкреатической энтодермы, имеющей около 96% или более клеток, которые являются положительными в отношении совместной экспрессии PDX1 и NKX6.1 и положительными в отношении экспрессии PTF1A.
6. Способ по п. 4, дополнительно включающий дифференцировку клеток панкреатической энтодермы, которые являются по существу отрицательными в отношении экспрессии PTF1A и NGN3, до панкреатических эндокринных клеток при отсутствии стадии дифференцировки, на которой формируются клетки, положительные в отношении экспрессии PTF1A.
7. Способ дифференцировки человеческих плюрипотентных клеток, включающий стадии: дифференцировки клеток энтодермы передней кишки в клетки панкреатической энтодермы посредством культивирования клеток энтодермы передней кишки в динамической суспензионной культуре при рН от около 7,2 до около 7,0 в течение, по меньшей мере, около 24 часов, при концентрации клеток, равной или превышающей около 1,5 миллиона клеток/мл и концентрации ретиноида от около 0,5 до около 1,0 мкМ, причем культивирование выполняют при отсутствии компонентов для одного или более из ингибирования, блокирования, активации или стимулирования сигнального пути TGF-бета и сигнального пути BMP и ингибитора сигнального пути белка sonic hedgehog.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462094509P | 2014-12-19 | 2014-12-19 | |
| US62/094,509 | 2014-12-19 | ||
| PCT/US2015/064713 WO2016100035A1 (en) | 2014-12-19 | 2015-12-09 | Suspension culturing of pluripotent stem cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2017125728A true RU2017125728A (ru) | 2019-01-22 |
| RU2017125728A3 RU2017125728A3 (ru) | 2019-06-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2017125728A RU2017125728A (ru) | 2014-12-19 | 2015-12-09 | Культивирование плюрипотентных стволовых клеток в суспензии |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US20160215268A1 (ru) |
| EP (1) | EP3234109A4 (ru) |
| JP (1) | JP6800854B2 (ru) |
| KR (3) | KR102281752B1 (ru) |
| CN (1) | CN107250349A (ru) |
| AR (1) | AR103080A1 (ru) |
| AU (1) | AU2015363008B2 (ru) |
| BR (1) | BR112017012974A2 (ru) |
| CA (1) | CA2970935A1 (ru) |
| MX (1) | MX2017008176A (ru) |
| PH (1) | PH12017501153A1 (ru) |
| RU (1) | RU2017125728A (ru) |
| SG (1) | SG11201704961VA (ru) |
| TW (1) | TW201636421A (ru) |
| WO (1) | WO2016100035A1 (ru) |
| ZA (1) | ZA201704868B (ru) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017127921A1 (en) | 2016-01-26 | 2017-08-03 | Centre For Commercialization Of Regenerative Medicine | Method for dissociating cell aggregates |
| US10391156B2 (en) | 2017-07-12 | 2019-08-27 | Viacyte, Inc. | University donor cells and related methods |
| EP3654996A4 (en) * | 2017-07-21 | 2021-03-24 | Semma Therapeutics, Inc. | REAGGREGATION OF PANCREAS BETA CELLS FROM STEM CELLS |
| CA3081762A1 (en) * | 2017-11-15 | 2019-05-23 | Semma Therapeutics, Inc. | Islet cell manufacturing compositions and methods of use |
| CA3108275A1 (en) | 2018-08-10 | 2020-02-13 | Vertex Pharmaceuticals Incorporated | Stem cell derived islet differentiation |
| US20200080107A1 (en) | 2018-09-07 | 2020-03-12 | Crispr Therapeutics Ag | Universal donor cells |
| JP7295576B2 (ja) * | 2018-09-19 | 2023-06-21 | オリヅルセラピューティクス株式会社 | インスリン産生細胞 |
| JP2022534545A (ja) | 2019-05-31 | 2022-08-01 | ダブリュ．エル．ゴア アンド アソシエイツ，インコーポレイティド | 生体適合性メンブレン複合体 |
| WO2020243668A1 (en) | 2019-05-31 | 2020-12-03 | W. L. Gore & Associates, Inc. | Cell encapsulation devices with controlled oxygen diffusion distances |
| EP3975926A1 (en) | 2019-05-31 | 2022-04-06 | W.L. Gore & Associates, Inc. | A biocompatible membrane composite |
| CA3139591C (en) | 2019-05-31 | 2024-01-16 | Timothy M. BRUHN | A biocompatible membrane composite |
| CN114364791A (zh) | 2019-09-05 | 2022-04-15 | 克里斯珀医疗股份公司 | 通用供体细胞 |
| US11104918B2 (en) | 2019-09-05 | 2021-08-31 | Crispr Therapeutics Ag | Universal donor cells |
| CA3203392A1 (en) | 2020-12-31 | 2022-07-07 | Alireza Rezania | Universal donor cells |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| CA2692325C (en) | 2001-12-07 | 2015-10-20 | Geron Corporation | Islet cells from human embryonic stem cells |
| JP4950660B2 (ja) | 2003-06-27 | 2012-06-13 | エチコン、インコーポレイテッド | 分娩後由来細胞を使用する眼組織の修復および再生 |
| AU2004309421B2 (en) | 2003-12-23 | 2011-04-21 | Viacyte, Inc. | Definitive endoderm |
| US20050266554A1 (en) | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
| CN1934245B (zh) | 2004-03-23 | 2012-07-04 | 第一三共株式会社 | 多能干细胞的增殖方法 |
| JP2008538276A (ja) | 2005-01-28 | 2008-10-23 | ノヴァセラ・リミテッド | 胚幹細胞培養のための方法 |
| AU2006202209B2 (en) | 2005-05-27 | 2011-04-14 | Lifescan, Inc. | Amniotic fluid derived cells |
| AU2006286149B2 (en) | 2005-08-29 | 2012-09-13 | Technion Research And Development Foundation Ltd. | Media for culturing stem cells |
| DK2674485T3 (da) * | 2005-10-27 | 2019-08-26 | Viacyte Inc | Pdx-1 udtrykkende dorsal og ventral fortarm endoderm |
| US7695965B2 (en) | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
| CA2644468C (en) * | 2006-03-02 | 2022-02-01 | Cythera, Inc. | Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production |
| WO2008013664A2 (en) | 2006-07-26 | 2008-01-31 | Cythera, Inc. | Methods of producing pancreatic hormones |
| DK2733203T3 (en) | 2006-08-02 | 2019-02-04 | Technion Res & Dev Foundation | PROCEDURES FOR EXPANSION OF EMBRYONAL STEM CELLS IN A SUSPENSION CULTURE |
| DK2173863T3 (en) | 2007-06-29 | 2019-01-21 | Fujifilm Cellular Dynamics Inc | Automated method and apparatus for embryonic stem cell culture |
| EP2310492B1 (en) * | 2008-06-30 | 2015-07-22 | Janssen Biotech, Inc. | Differentiation of pluripotent stem cells |
| US9683215B2 (en) * | 2008-08-22 | 2017-06-20 | President And Fellows Of Harvard College | Methods of reprogramming cells |
| AU2010319921A1 (en) | 2009-10-29 | 2012-05-17 | Janssen Biotech Inc. | Pluripotent stem cells |
| EP2563908B1 (en) * | 2010-04-25 | 2019-01-09 | Icahn School of Medicine at Mount Sinai | Generation of anterior foregut endoderm from pluripotent cells |
| BR112014015417A8 (pt) * | 2011-12-22 | 2017-07-04 | Janssen Biotech Inc | diferenciação de células-tronco embrionárias humanas em células positivas de insulina hormonal únicas |
| MX2015008578A (es) * | 2012-12-31 | 2015-09-07 | Janssen Biotech Inc | Diferenciacion de celulas madre embrionarias humanas en celulas endocrinas pancreaticas mediante el uso de relugadores de hb9. |
| US8859286B2 (en) | 2013-03-14 | 2014-10-14 | Viacyte, Inc. | In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells |
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2015
- 2015-12-09 EP EP15870718.2A patent/EP3234109A4/en active Pending
- 2015-12-09 JP JP2017532641A patent/JP6800854B2/ja active Active
- 2015-12-09 WO PCT/US2015/064713 patent/WO2016100035A1/en active Application Filing
- 2015-12-09 US US14/963,730 patent/US20160215268A1/en not_active Abandoned
- 2015-12-09 RU RU2017125728A patent/RU2017125728A/ru not_active Application Discontinuation
- 2015-12-09 AU AU2015363008A patent/AU2015363008B2/en active Active
- 2015-12-09 BR BR112017012974A patent/BR112017012974A2/pt not_active Application Discontinuation
- 2015-12-09 KR KR1020177019548A patent/KR102281752B1/ko active IP Right Grant
- 2015-12-09 CN CN201580076646.0A patent/CN107250349A/zh active Pending
- 2015-12-09 KR KR1020217022958A patent/KR102519502B1/ko active IP Right Grant
- 2015-12-09 KR KR1020237011455A patent/KR20230050478A/ko active IP Right Grant
- 2015-12-09 SG SG11201704961VA patent/SG11201704961VA/en unknown
- 2015-12-09 MX MX2017008176A patent/MX2017008176A/es unknown
- 2015-12-09 CA CA2970935A patent/CA2970935A1/en active Pending
- 2015-12-17 AR ARP150104154A patent/AR103080A1/es unknown
- 2015-12-17 TW TW104142402A patent/TW201636421A/zh unknown
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2017
- 2017-06-19 PH PH12017501153A patent/PH12017501153A1/en unknown
- 2017-07-18 ZA ZA2017/04868A patent/ZA201704868B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20160215268A1 (en) | 2016-07-28 |
| ZA201704868B (en) | 2019-02-27 |
| CA2970935A1 (en) | 2016-06-23 |
| JP6800854B2 (ja) | 2020-12-16 |
| AU2015363008A1 (en) | 2017-06-29 |
| PH12017501153A1 (en) | 2017-11-27 |
| RU2017125728A3 (ru) | 2019-06-03 |
| AR103080A1 (es) | 2017-04-12 |
| EP3234109A4 (en) | 2018-06-27 |
| TW201636421A (zh) | 2016-10-16 |
| AU2015363008B2 (en) | 2021-12-09 |
| MX2017008176A (es) | 2018-02-09 |
| SG11201704961VA (en) | 2017-07-28 |
| KR20230050478A (ko) | 2023-04-14 |
| JP2017537649A (ja) | 2017-12-21 |
| KR102281752B1 (ko) | 2021-07-23 |
| KR20170087520A (ko) | 2017-07-28 |
| BR112017012974A2 (pt) | 2018-01-09 |
| EP3234109A1 (en) | 2017-10-25 |
| CN107250349A (zh) | 2017-10-13 |
| WO2016100035A1 (en) | 2016-06-23 |
| KR20210094141A (ko) | 2021-07-28 |
| KR102519502B1 (ko) | 2023-04-06 |
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