JP2019501638A - ニコチンアミドリボシドの微生物生産 - Google Patents

ニコチンアミドリボシドの微生物生産 Download PDF

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JP2019501638A
JP2019501638A JP2018523761A JP2018523761A JP2019501638A JP 2019501638 A JP2019501638 A JP 2019501638A JP 2018523761 A JP2018523761 A JP 2018523761A JP 2018523761 A JP2018523761 A JP 2018523761A JP 2019501638 A JP2019501638 A JP 2019501638A
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activity
protein
polypeptide
seq
nicotinamide
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JP2019501638A5 (enExample
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アダム ジー． ローレンス，
アダム ジー． ローレンス，
セリーヌ ヴィアルージュ，
セリーヌ ヴィアルージュ，
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DSM IP Assets BV
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    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01005—NAD+ synthase (6.3.1.5)
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JP2018523761A 2015-11-13 2016-11-14 ニコチンアミドリボシドの微生物生産 Pending JP2019501638A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562254736P 2015-11-13 2015-11-13
US62/254,736 2015-11-13
PCT/US2016/061905 WO2017083858A2 (en) 2015-11-13 2016-11-14 Microbial production of nicotinamide riboside

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JP2019501638A true JP2019501638A (ja) 2019-01-24
JP2019501638A5 JP2019501638A5 (enExample) 2019-12-12

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US (1) US10913965B2 (enExample)
EP (1) EP3374492A4 (enExample)
JP (1) JP2019501638A (enExample)
KR (1) KR20180083350A (enExample)
CN (1) CN108473948A (enExample)
BR (1) BR112018009393A8 (enExample)
WO (1) WO2017083858A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021070829A1 (ja) * 2019-10-11 2021-04-15 国立大学法人静岡大学 ニコチンアミドリボシドを産生する乳酸菌、並びにニコチンアミドモノヌクレオチド及びニコチンアミドリボシドを産生する乳酸菌
US11959116B2 (en) 2021-10-27 2024-04-16 Asahi Kasei Pharma Corporation Method for producing nicotinamide mononucleotide

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108473948A (zh) * 2015-11-13 2018-08-31 帝斯曼知识产权资产管理有限公司 烟酰胺核糖苷的微生物生产
WO2018211028A1 (en) * 2017-05-18 2018-11-22 Dsm Ip Assets B.V. Microbial production of nicotinamide riboside
WO2019065876A1 (ja) * 2017-09-29 2019-04-04 三菱ケミカル株式会社 ニコチンアミドモノヌクレオチドの製造方法およびその方法に用いる形質転換体
EP3877515A1 (en) * 2018-11-09 2021-09-15 Wageningen Universiteit Microbial hosts engineered for increased tolerance to temperature shifts
CN113755415B (zh) * 2020-06-04 2024-03-29 苏州华赛生物工程技术有限公司 一种新的具有nmn合成路径的重组微生物及生产方法
CN112359082B (zh) * 2020-11-13 2023-02-21 南京工业大学 一种烟酰胺单核苷酸的制备方法
CN112501193B (zh) * 2020-12-09 2022-09-27 江南大学 一种烟酸及烟酰胺生物传感系统
CN112795582B (zh) * 2021-01-22 2022-12-13 江南大学 一种适合在微生物中高效合成nad衍生物的酶基因
CN112852678B (zh) * 2021-03-08 2021-11-05 泓博元生命科技（深圳）有限公司 一株产烟酰胺单核苷酸的成都肠杆菌及其应用
CN112980906B (zh) * 2021-04-14 2021-07-30 深圳瑞德林生物技术有限公司 一种用于制备β-烟酰胺单核苷酸的酶组合物及其应用
CN112795606B (zh) * 2021-04-14 2021-07-27 深圳瑞德林生物技术有限公司 一种β-烟酰胺单核苷酸的酶催化合成方法
CN113528562B (zh) * 2021-06-23 2022-08-16 苏州华赛生物工程技术有限公司 生产β-烟酰胺核糖的重组微生物及其构建方法和应用
CN113897382B (zh) * 2021-09-01 2023-10-20 浙江工业大学 辅酶自足型大肠杆菌及其构建方法与应用
CN114231477B (zh) * 2021-12-27 2024-03-15 天津科技大学 高产β-烟酰胺单核苷酸的基因工程菌株及其构建与应用
CN114854656B (zh) * 2022-05-05 2023-07-18 江南大学 一种产烟酰胺核糖苷的重组菌
CN116286725B (zh) * 2023-03-23 2025-12-12 赛诺根生物科学有限公司 一种催化性能提高的nadh焦磷酸酶突变体及其应用
CN118773286B (zh) * 2024-09-10 2025-02-14 中国科学院深圳先进技术研究院 用于检测qprt活性的组合物、qprt活性的检测方法与应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090202680A1 (en) * 2004-02-10 2009-08-13 Charles Brenner Yeast strain and method for using the same to produce nicotinamide riboside

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102154339A (zh) * 2011-02-16 2011-08-17 南京工业大学 一种产丁二酸大肠杆菌基因工程菌株的构建方法
WO2015069860A1 (en) * 2013-11-06 2015-05-14 President And Fellows Of Harvard College Biological production of nad precursors and analogs
CN108473948A (zh) * 2015-11-13 2018-08-31 帝斯曼知识产权资产管理有限公司 烟酰胺核糖苷的微生物生产

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090202680A1 (en) * 2004-02-10 2009-08-13 Charles Brenner Yeast strain and method for using the same to produce nicotinamide riboside

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
APPL ENVIRON MICROBIOL, 2011 SEP, VOL. 77, NO. 17, PP. 6133-6140, JPN6020040165, ISSN: 0004592978 *
MICROBIOL MOL BIOL REV, 2009 SEP, VOL. 73, NO. 3, PP. 529-541, JPN6020040171, ISSN: 0004371708 *
PROC NATL ACAD SCI U S A, 2009 MAR 3, VOL. 106, NO. 9, PP. 3083-3088, JPN6020040167, ISSN: 0004592979 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021070829A1 (ja) * 2019-10-11 2021-04-15 国立大学法人静岡大学 ニコチンアミドリボシドを産生する乳酸菌、並びにニコチンアミドモノヌクレオチド及びニコチンアミドリボシドを産生する乳酸菌
JPWO2021070829A1 (enExample) * 2019-10-11 2021-04-15
JP2025072615A (ja) * 2019-10-11 2025-05-09 国立大学法人静岡大学 ニコチンアミドリボシドを産生する乳酸菌、並びにニコチンアミドモノヌクレオチド及びニコチンアミドリボシドを産生する乳酸菌
JP7684906B2 (ja) 2019-10-11 2025-05-28 国立大学法人静岡大学 ニコチンアミドリボシドを産生する乳酸菌、並びにニコチンアミドモノヌクレオチド及びニコチンアミドリボシドを産生する乳酸菌
US11959116B2 (en) 2021-10-27 2024-04-16 Asahi Kasei Pharma Corporation Method for producing nicotinamide mononucleotide

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EP3374492A4 (en) 2019-07-24
US20180327797A1 (en) 2018-11-15
CN108473948A (zh) 2018-08-31
WO2017083858A3 (en) 2017-06-29
BR112018009393A8 (pt) 2019-02-26
WO2017083858A2 (en) 2017-05-18
US10913965B2 (en) 2021-02-09
EP3374492A2 (en) 2018-09-19
KR20180083350A (ko) 2018-07-20
BR112018009393A2 (pt) 2018-11-13

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