JP2018532373A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2018532373A5 JP2018532373A5 JP2018506840A JP2018506840A JP2018532373A5 JP 2018532373 A5 JP2018532373 A5 JP 2018532373A5 JP 2018506840 A JP2018506840 A JP 2018506840A JP 2018506840 A JP2018506840 A JP 2018506840A JP 2018532373 A5 JP2018532373 A5 JP 2018532373A5
- Authority
- JP
- Japan
- Prior art keywords
- cell
- nucleic acid
- chromatin
- transposase
- sample containing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 18
- 210000004027 cell Anatomy 0.000 claims 12
- 150000007523 nucleic acids Chemical class 0.000 claims 12
- 108020004707 nucleic acids Proteins 0.000 claims 11
- 102000039446 nucleic acids Human genes 0.000 claims 11
- 108010077544 Chromatin Proteins 0.000 claims 9
- 210000003483 chromatin Anatomy 0.000 claims 9
- 108010033040 Histones Proteins 0.000 claims 7
- 206010028980 Neoplasm Diseases 0.000 claims 5
- 108010020764 Transposases Proteins 0.000 claims 4
- 102000008579 Transposases Human genes 0.000 claims 4
- 239000011230 binding agent Substances 0.000 claims 4
- 238000002955 isolation Methods 0.000 claims 4
- 239000000126 substance Substances 0.000 claims 4
- 102000006947 Histones Human genes 0.000 claims 3
- 108091023040 Transcription factor Proteins 0.000 claims 3
- 102000040945 Transcription factor Human genes 0.000 claims 3
- 210000004102 animal cell Anatomy 0.000 claims 3
- 201000011510 cancer Diseases 0.000 claims 3
- 210000005260 human cell Anatomy 0.000 claims 3
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 201000010099 disease Diseases 0.000 claims 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 2
- 238000000527 sonication Methods 0.000 claims 2
- 210000001519 tissue Anatomy 0.000 claims 2
- 102000011682 Centromere Protein A Human genes 0.000 claims 1
- 108010076303 Centromere Protein A Proteins 0.000 claims 1
- 108020004414 DNA Proteins 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108010012306 Tn5 transposase Proteins 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 230000021736 acetylation Effects 0.000 claims 1
- 238000006640 acetylation reaction Methods 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 210000000601 blood cell Anatomy 0.000 claims 1
- 230000006242 butyrylation Effects 0.000 claims 1
- 238000010514 butyrylation reaction Methods 0.000 claims 1
- 238000007444 cell Immobilization Methods 0.000 claims 1
- 238000004113 cell culture Methods 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 238000004132 cross linking Methods 0.000 claims 1
- 238000010614 crotonylation reaction Methods 0.000 claims 1
- 230000009089 cytolysis Effects 0.000 claims 1
- 239000003814 drug Substances 0.000 claims 1
- 229940079593 drug Drugs 0.000 claims 1
- 201000005787 hematologic cancer Diseases 0.000 claims 1
- 208000024200 hematopoietic and lymphoid system neoplasm Diseases 0.000 claims 1
- 210000002865 immune cell Anatomy 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
- 230000009878 intermolecular interaction Effects 0.000 claims 1
- 208000032839 leukemia Diseases 0.000 claims 1
- 230000017538 malonylation Effects 0.000 claims 1
- 238000013507 mapping Methods 0.000 claims 1
- 230000011987 methylation Effects 0.000 claims 1
- 238000007069 methylation reaction Methods 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 230000006289 propionylation Effects 0.000 claims 1
- 238000010515 propionylation reaction Methods 0.000 claims 1
- 238000011084 recovery Methods 0.000 claims 1
- 238000007634 remodeling Methods 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 210000000130 stem cell Anatomy 0.000 claims 1
- 230000035322 succinylation Effects 0.000 claims 1
- 238000010613 succinylation reaction Methods 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15180705 | 2015-08-12 | ||
| EP15180705.4 | 2015-08-12 | ||
| EP15189788.1 | 2015-10-14 | ||
| EP15189788 | 2015-10-14 | ||
| PCT/EP2016/069121 WO2017025594A1 (en) | 2015-08-12 | 2016-08-11 | Methods for studying nucleic acids |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018532373A JP2018532373A (ja) | 2018-11-08 |
| JP2018532373A5 true JP2018532373A5 (https=) | 2019-09-19 |
| JP6697070B2 JP6697070B2 (ja) | 2020-05-20 |
Family
ID=56876995
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018506840A Active JP6697070B2 (ja) | 2015-08-12 | 2016-08-11 | 核酸の研究方法 |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US10934636B2 (https=) |
| EP (2) | EP3674414A1 (https=) |
| JP (1) | JP6697070B2 (https=) |
| CN (1) | CN108368540B (https=) |
| CA (1) | CA2995305A1 (https=) |
| CY (1) | CY1122632T1 (https=) |
| DK (1) | DK3334841T3 (https=) |
| ES (1) | ES2779798T3 (https=) |
| HR (1) | HRP20200062T1 (https=) |
| HU (1) | HUE047175T2 (https=) |
| LT (1) | LT3334841T (https=) |
| PL (1) | PL3334841T3 (https=) |
| PT (1) | PT3334841T (https=) |
| RS (1) | RS59838B1 (https=) |
| SI (1) | SI3334841T1 (https=) |
| SM (1) | SMT202000037T1 (https=) |
| WO (1) | WO2017025594A1 (https=) |
Families Citing this family (86)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0719620D0 (en) | 2007-10-08 | 2007-11-14 | Isis Innovation | Mutant Enzymes |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US10689643B2 (en) | 2011-11-22 | 2020-06-23 | Active Motif, Inc. | Targeted transposition for use in epigenetic studies |
| US9938524B2 (en) | 2011-11-22 | 2018-04-10 | Active Motif, Inc. | Multiplex isolation of protein-associated nucleic acids |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| KR20200140929A (ko) | 2013-02-08 | 2020-12-16 | 10엑스 제노믹스, 인크. | 폴리뉴클레오티드 바코드 생성 |
| SG11201508985VA (en) | 2013-05-23 | 2015-12-30 | Univ Leland Stanford Junior | Transposition into native chromatin for personal epigenomics |
| BR112016023625A2 (pt) | 2014-04-10 | 2018-06-26 | 10X Genomics, Inc. | dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos |
| WO2015200893A2 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| CA2953469A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| BR112017008877A2 (pt) | 2014-10-29 | 2018-07-03 | 10X Genomics Inc | métodos e composições para sequenciamento de ácido nucleico-alvo |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| JP6769969B2 (ja) | 2015-01-12 | 2020-10-14 | １０エックス ジェノミクス， インコーポレイテッド | 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー |
| MX2017010857A (es) | 2015-02-24 | 2017-12-11 | 10X Genomics Inc | Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana. |
| EP3262407B1 (en) | 2015-02-24 | 2023-08-30 | 10X Genomics, Inc. | Partition processing methods and systems |
| JP6954899B2 (ja) | 2015-12-04 | 2021-10-27 | １０エックス ゲノミクス，インコーポレイテッド | 核酸の解析のための方法及び組成物 |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP4029939B1 (en) | 2017-01-30 | 2023-06-28 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| US10914729B2 (en) | 2017-05-22 | 2021-02-09 | The Trustees Of Princeton University | Methods for detecting protein binding sequences and tagging nucleic acids |
| US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| EP3445876B1 (en) | 2017-05-26 | 2023-07-05 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| WO2019018385A1 (en) * | 2017-07-17 | 2019-01-24 | The Board Of Trustees Of The Leland Stanford Junior University | EPIGENETIC PROFILING USING THE TARGETED LIGATION OF CHROMATIN |
| DK4060041T5 (en) * | 2017-09-25 | 2024-07-22 | Fred Hutchinson Cancer Center | High efficiency targeted in situ genome-wide profiling |
| WO2019076768A1 (en) * | 2017-10-16 | 2019-04-25 | Tervisetehnoloogiate Arenduskeskus As | METHOD AND KIT FOR PREPARING DNA BANK |
| WO2019084043A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS |
| CN107841533B (zh) * | 2017-11-13 | 2021-11-05 | 深圳先进技术研究院 | 一种染色质破碎方法及其应用 |
| SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| CN108300774A (zh) * | 2017-12-01 | 2018-07-20 | 苏州睿迈英基因检测科技有限公司 | 一种改进的开放染色质检测方法及其试剂盒和应用 |
| WO2019116306A1 (en) | 2017-12-13 | 2019-06-20 | Ecole Polytechnique Federale De Lausanne (Epfl) | System for capture or immunoprecipitation of a protein-dna complex |
| CN108220394B (zh) * | 2018-01-05 | 2021-03-23 | 清华大学 | 基因调控性染色质相互作用的鉴定方法、系统及其应用 |
| US12377396B2 (en) | 2018-02-05 | 2025-08-05 | The Board Of Trustees Of The Leland Stanford Junior University | Systems and methods for multiplexed measurements in single and ensemble cells |
| CN108285494B (zh) * | 2018-02-11 | 2021-09-07 | 北京大学 | 一种融合蛋白、试剂盒以及CHIP-seq检测方法 |
| SG11202007686VA (en) | 2018-02-12 | 2020-09-29 | 10X Genomics Inc | Methods characterizing multiple analytes from individual cells or cell populations |
| CN112262218B (zh) | 2018-04-06 | 2024-11-08 | 10X基因组学有限公司 | 用于单细胞处理中的质量控制的系统和方法 |
| CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
| US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
| CN108913712B (zh) * | 2018-07-17 | 2021-04-02 | 厦门生命互联科技有限公司 | 一种重组Tn5转座酶的表达纯化方法 |
| SG11202101164TA (en) | 2018-08-03 | 2021-03-30 | 10X Genomics Inc | Methods and systems to minimize barcode exchange |
| US12065688B2 (en) | 2018-08-20 | 2024-08-20 | 10X Genomics, Inc. | Compositions and methods for cellular processing |
| CN111269284B (zh) * | 2018-12-04 | 2023-07-25 | 中国科学院大连化学物理研究所 | 同步分离细胞质和细胞核中蛋白质和rna的试剂及方法 |
| CN112867800B (zh) * | 2018-12-17 | 2024-07-12 | Illumina公司 | 用于制备测序文库的方法和手段 |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| CN111440843A (zh) * | 2019-01-16 | 2020-07-24 | 中国科学院生物物理研究所 | 一种利用微量临床穿刺样本进行染色质免疫共沉淀文库制备的方法及其应用 |
| CN113661250A (zh) * | 2019-02-11 | 2021-11-16 | 爱披萨佛公司 | 使用长读长测序的染色质作图测定和试剂盒 |
| WO2020167866A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transposon loading |
| EP3924505B1 (en) | 2019-02-12 | 2025-12-17 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| US11467153B2 (en) | 2019-02-12 | 2022-10-11 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| EP3924507A4 (en) * | 2019-02-15 | 2022-11-30 | Epicypher, Inc. | QUANTITATIVE IMAGE OF CHROMATIN-ASSOCIATED PROTEINS |
| CN109880887A (zh) * | 2019-03-20 | 2019-06-14 | 嘉兴菲沙基因信息有限公司 | 一种适用于植物ATAC-seq测序技术的文库构建方法 |
| WO2020198664A1 (en) * | 2019-03-28 | 2020-10-01 | The Regents Of The University Of California | Methods and systems for sequencing cell-free microbiome dna |
| KR102192455B1 (ko) * | 2019-04-05 | 2020-12-17 | 한국과학기술원 | 개인의 전이효소-접근가능한 염색질 시퀀싱 정보를 이용한 암 진단 마커 및 이의 용도 |
| WO2021011433A1 (en) * | 2019-07-12 | 2021-01-21 | New York Genome Center, Inc | Methods and compositions for scalable pooled rna screens with single cell chromatin accessibility profiling |
| EP3999624B1 (en) | 2019-07-16 | 2026-03-25 | Meliolabs Inc. | Methods and devices for single-cell based digital high resolution melt |
| US12571051B2 (en) * | 2019-08-27 | 2026-03-10 | Belgian Volition Srl | Method of isolating circulating nucleosomes |
| KR20220080091A (ko) | 2019-09-06 | 2022-06-14 | 체엠엠 - 포르슝스첸트룸 퓨어 몰레쿨라레 메디친 게엠베하 | Rna 올리고뉴클레오티드를 시퀀싱하기 위한 방법 |
| EP4081656A1 (en) * | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| CN115135984B (zh) | 2019-12-23 | 2025-12-23 | 10X基因组学有限公司 | 可逆固定试剂及其使用方法 |
| WO2021146219A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Cell capture using du-containing oligonucleotides |
| CN115279917B (zh) * | 2020-09-16 | 2025-10-10 | 深圳华大生命科学研究院 | 多维度解析细胞表观基因组学的方法 |
| TW202242130A (zh) * | 2020-12-29 | 2022-11-01 | 比利時商比利時意志有限公司 | 循環轉錄因子分析 |
| EP4288534A1 (en) * | 2021-02-05 | 2023-12-13 | Ospedale San Raffaele S.r.l. | Engineered transposase and uses thereof |
| CN112795563A (zh) * | 2021-03-23 | 2021-05-14 | 上海欣百诺生物科技有限公司 | 生物素化的转座体在回收CUT&Tag或ATAC-seq产物中的用途及方法 |
| CN113186174B (zh) * | 2021-04-23 | 2022-04-15 | 江南大学 | 一种Tn5突变酶的制备和应用 |
| CN113774052A (zh) * | 2021-09-30 | 2021-12-10 | 首都医科大学附属北京安贞医院 | mtDNA的提取和建库试剂 |
| JP2025523739A (ja) * | 2022-07-27 | 2025-07-25 | イルミナ インコーポレイテッド | タグメンテーションワークフロー |
| CN115948363B (zh) * | 2022-08-26 | 2024-02-27 | 武汉影子基因科技有限公司 | Tn5转座酶突变体及其制备方法和应用 |
| CN116590283B (zh) * | 2023-07-17 | 2023-11-07 | 北京微岩医疗器械有限公司 | 一种细胞结合增强剂和去除人源游离核酸的方法 |
| CN117106857B (zh) * | 2023-10-24 | 2024-02-09 | 北京大学 | 一种血浆游离染色质的检测方法、试剂盒及其应用 |
| WO2025151541A1 (en) * | 2024-01-08 | 2025-07-17 | Massachusetts Eye And Ear Infirmary | Extraction of genomic dna from fixed cells |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| US5965443A (en) | 1996-09-09 | 1999-10-12 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
| US5925545A (en) | 1996-09-09 | 1999-07-20 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
| US6159736A (en) | 1998-09-23 | 2000-12-12 | Wisconsin Alumni Research Foundation | Method for making insertional mutations using a Tn5 synaptic complex |
| US6406896B1 (en) | 1999-08-02 | 2002-06-18 | Wisconsin Alumni Research Foundation | Transposase enzyme and method for use |
| US7067644B2 (en) | 2000-12-05 | 2006-06-27 | Wisconsin Alumni Research Foundation | Double transposition methods for manipulating nucleic acids |
| US7527966B2 (en) | 2002-06-26 | 2009-05-05 | Transgenrx, Inc. | Gene regulation in transgenic animals using a transposon-based vector |
| US7316903B2 (en) | 2003-03-28 | 2008-01-08 | United States Of America As Represented By The Department Of Health And Human Services | Detection of nucleic acid sequence variations using phase Mu transposase |
| WO2004093645A2 (en) | 2003-04-17 | 2004-11-04 | Wisconsin Alumni Research Foundation | Tn5 transposase mutants and the use thereof |
| US7608434B2 (en) | 2004-08-04 | 2009-10-27 | Wisconsin Alumni Research Foundation | Mutated Tn5 transposase proteins and the use thereof |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| WO2012103545A1 (en) | 2011-01-28 | 2012-08-02 | Illumina, Inc. | Oligonucleotide replacement for di-tagged and directional libraries |
| WO2013078470A2 (en) | 2011-11-22 | 2013-05-30 | MOTIF, Active | Multiplex isolation of protein-associated nucleic acids |
| CN102703426A (zh) * | 2012-05-21 | 2012-10-03 | 吴江汇杰生物科技有限公司 | 构建核酸库的方法、试剂及试剂盒 |
| DK2999784T4 (en) * | 2013-05-22 | 2023-02-20 | Active Motif Inc | Målrettet transposition til anvendelse i epigenetiske studier |
| US20160208323A1 (en) | 2013-06-21 | 2016-07-21 | The Broad Institute, Inc. | Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing |
-
2016
- 2016-08-11 LT LTEP16760659.9T patent/LT3334841T/lt unknown
- 2016-08-11 DK DK16760659.9T patent/DK3334841T3/da active
- 2016-08-11 EP EP19205821.2A patent/EP3674414A1/en not_active Withdrawn
- 2016-08-11 US US15/751,918 patent/US10934636B2/en active Active
- 2016-08-11 HR HRP20200062TT patent/HRP20200062T1/hr unknown
- 2016-08-11 PL PL16760659T patent/PL3334841T3/pl unknown
- 2016-08-11 CN CN201680058837.9A patent/CN108368540B/zh not_active Expired - Fee Related
- 2016-08-11 PT PT167606599T patent/PT3334841T/pt unknown
- 2016-08-11 HU HUE16760659A patent/HUE047175T2/hu unknown
- 2016-08-11 SI SI201630614T patent/SI3334841T1/sl unknown
- 2016-08-11 CA CA2995305A patent/CA2995305A1/en active Pending
- 2016-08-11 RS RS20200057A patent/RS59838B1/sr unknown
- 2016-08-11 ES ES16760659T patent/ES2779798T3/es active Active
- 2016-08-11 EP EP16760659.9A patent/EP3334841B1/en active Active
- 2016-08-11 WO PCT/EP2016/069121 patent/WO2017025594A1/en not_active Ceased
- 2016-08-11 SM SM20200037T patent/SMT202000037T1/it unknown
- 2016-08-11 JP JP2018506840A patent/JP6697070B2/ja active Active
-
2020
- 2020-01-22 CY CY20201100053T patent/CY1122632T1/el unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2018532373A5 (https=) | ||
| HRP20200062T1 (hr) | Postupak proučavanja nukleinskih kiselina | |
| Darnell | CLIP (cross-linking and immunoprecipitation) identification of RNAs bound by a specific protein | |
| US20250043366A1 (en) | Tiled assays using crispr-cas based detection | |
| CN116157533A (zh) | 使用杂交方法捕获遗传靶标 | |
| Issac et al. | Proteomic profiling of chikungunya virus-infected human muscle cells: Reveal the role of cytoskeleton network in CHIKV replication | |
| JP2020506671A (ja) | 細胞区画内の生体分子への直交アクセスおよび細胞区画内の生体分子のタグ付けのための分析システム | |
| MX2023008931A (es) | Complejos de transposoma ligados a superficie de complejo. | |
| JP2019522487A (ja) | 保存されたサンプルからの長距離連鎖情報の回復 | |
| WO2014125668A1 (ja) | 内在性dna配列特異的結合分子を用いる特定ゲノム領域の単離方法 | |
| GB2577214A (en) | Methods for trapping and barcoding discrete biological units in hydrogel | |
| Texari et al. | An optimized protocol for rapid, sensitive and robust on-bead ChIP-seq from primary cells | |
| Bai et al. | Development of a DNA‐templated peptide probe for photoaffinity labeling and enrichment of the histone modification reader proteins | |
| CN106801096B (zh) | 一种染色质免疫共沉淀样品快速制备方法 | |
| AU2021411520A1 (en) | Methods and compositions for sequencing library preparation | |
| JP2019017281A (ja) | タンパク質−ｒｎａ相互作用の検出 | |
| Singh et al. | A novel approach for extracting DNA from formalin-fixed paraffin-embedded tissue using microwave | |
| Henikoff et al. | In situ tools for chromatin structural epigenomics | |
| Dabrowska et al. | Efficacy of antiviral drugs against the omicron variant of SARS-CoV-2 | |
| Baranello et al. | Mapping DNA breaks by next-generation sequencing | |
| Colling et al. | cDNA-AFLP-based transcript profiling for genome-wide expression analysis of jasmonate-treated plants and plant cultures | |
| CN103789430B (zh) | 一种快速检测血液中附红细胞体、钩端螺旋体、弓形虫的试剂盒及应用 | |
| JP2017086027A5 (https=) | ||
| Marr et al. | Whole-genome methods to define DNA and histone accessibility and long-range interactions in chromatin | |
| CN105400884A (zh) | 一种基于rpa检测检疫性茄属杂草银毛龙葵的方法 |