JP6697070B2 - 核酸の研究方法 - Google Patents

核酸の研究方法 Download PDF

Info

Publication number
JP6697070B2
JP6697070B2 JP2018506840A JP2018506840A JP6697070B2 JP 6697070 B2 JP6697070 B2 JP 6697070B2 JP 2018506840 A JP2018506840 A JP 2018506840A JP 2018506840 A JP2018506840 A JP 2018506840A JP 6697070 B2 JP6697070 B2 JP 6697070B2
Authority
JP
Japan
Prior art keywords
chromatin
dna
cells
beads
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018506840A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018532373A5 (https=
JP2018532373A (ja
Inventor
クリストフ、ボック
クリスティアン、シュミードル
Original Assignee
ツェーエーエムエム・フォルシュングスツェントルム・フュア・モレクラーレ・メディツィン・ゲーエムベーハー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ツェーエーエムエム・フォルシュングスツェントルム・フュア・モレクラーレ・メディツィン・ゲーエムベーハー filed Critical ツェーエーエムエム・フォルシュングスツェントルム・フュア・モレクラーレ・メディツィン・ゲーエムベーハー
Publication of JP2018532373A publication Critical patent/JP2018532373A/ja
Publication of JP2018532373A5 publication Critical patent/JP2018532373A5/ja
Application granted granted Critical
Publication of JP6697070B2 publication Critical patent/JP6697070B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/04—Methods of creating libraries, e.g. combinatorial synthesis using dynamic combinatorial chemistry techniques
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/08—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/327—RNAse, e.g. RNAseH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10—Nucleic acid binding proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Structural Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
JP2018506840A 2015-08-12 2016-08-11 核酸の研究方法 Active JP6697070B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP15180705 2015-08-12
EP15180705.4 2015-08-12
EP15189788.1 2015-10-14
EP15189788 2015-10-14
PCT/EP2016/069121 WO2017025594A1 (en) 2015-08-12 2016-08-11 Methods for studying nucleic acids

Publications (3)

Publication Number Publication Date
JP2018532373A JP2018532373A (ja) 2018-11-08
JP2018532373A5 JP2018532373A5 (https=) 2019-09-19
JP6697070B2 true JP6697070B2 (ja) 2020-05-20

Family

ID=56876995

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2018506840A Active JP6697070B2 (ja) 2015-08-12 2016-08-11 核酸の研究方法

Country Status (17)

Country Link
US (1) US10934636B2 (https=)
EP (2) EP3674414A1 (https=)
JP (1) JP6697070B2 (https=)
CN (1) CN108368540B (https=)
CA (1) CA2995305A1 (https=)
CY (1) CY1122632T1 (https=)
DK (1) DK3334841T3 (https=)
ES (1) ES2779798T3 (https=)
HR (1) HRP20200062T1 (https=)
HU (1) HUE047175T2 (https=)
LT (1) LT3334841T (https=)
PL (1) PL3334841T3 (https=)
PT (1) PT3334841T (https=)
RS (1) RS59838B1 (https=)
SI (1) SI3334841T1 (https=)
SM (1) SMT202000037T1 (https=)
WO (1) WO2017025594A1 (https=)

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0719620D0 (en) 2007-10-08 2007-11-14 Isis Innovation Mutant Enzymes
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10689643B2 (en) 2011-11-22 2020-06-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
US9938524B2 (en) 2011-11-22 2018-04-10 Active Motif, Inc. Multiplex isolation of protein-associated nucleic acids
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20200140929A (ko) 2013-02-08 2020-12-16 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
SG11201508985VA (en) 2013-05-23 2015-12-30 Univ Leland Stanford Junior Transposition into native chromatin for personal epigenomics
BR112016023625A2 (pt) 2014-04-10 2018-06-26 10X Genomics, Inc. dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos
WO2015200893A2 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
BR112017008877A2 (pt) 2014-10-29 2018-07-03 10X Genomics Inc métodos e composições para sequenciamento de ácido nucleico-alvo
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
JP6769969B2 (ja) 2015-01-12 2020-10-14 １０エックス ジェノミクス， インコーポレイテッド 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー
MX2017010857A (es) 2015-02-24 2017-12-11 10X Genomics Inc Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana.
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
JP6954899B2 (ja) 2015-12-04 2021-10-27 １０エックス ゲノミクス，インコーポレイテッド 核酸の解析のための方法及び組成物
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3445876B1 (en) 2017-05-26 2023-07-05 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2019018385A1 (en) * 2017-07-17 2019-01-24 The Board Of Trustees Of The Leland Stanford Junior University EPIGENETIC PROFILING USING THE TARGETED LIGATION OF CHROMATIN
DK4060041T5 (en) * 2017-09-25 2024-07-22 Fred Hutchinson Cancer Center High efficiency targeted in situ genome-wide profiling
WO2019076768A1 (en) * 2017-10-16 2019-04-25 Tervisetehnoloogiate Arenduskeskus As METHOD AND KIT FOR PREPARING DNA BANK
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN107841533B (zh) * 2017-11-13 2021-11-05 深圳先进技术研究院 一种染色质破碎方法及其应用
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN108300774A (zh) * 2017-12-01 2018-07-20 苏州睿迈英基因检测科技有限公司 一种改进的开放染色质检测方法及其试剂盒和应用
WO2019116306A1 (en) 2017-12-13 2019-06-20 Ecole Polytechnique Federale De Lausanne (Epfl) System for capture or immunoprecipitation of a protein-dna complex
CN108220394B (zh) * 2018-01-05 2021-03-23 清华大学 基因调控性染色质相互作用的鉴定方法、系统及其应用
US12377396B2 (en) 2018-02-05 2025-08-05 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods for multiplexed measurements in single and ensemble cells
CN108285494B (zh) * 2018-02-11 2021-09-07 北京大学 一种融合蛋白、试剂盒以及CHIP-seq检测方法
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
CN108913712B (zh) * 2018-07-17 2021-04-02 厦门生命互联科技有限公司 一种重组Tn5转座酶的表达纯化方法
SG11202101164TA (en) 2018-08-03 2021-03-30 10X Genomics Inc Methods and systems to minimize barcode exchange
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
CN111269284B (zh) * 2018-12-04 2023-07-25 中国科学院大连化学物理研究所 同步分离细胞质和细胞核中蛋白质和rna的试剂及方法
CN112867800B (zh) * 2018-12-17 2024-07-12 Illumina公司 用于制备测序文库的方法和手段
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
CN111440843A (zh) * 2019-01-16 2020-07-24 中国科学院生物物理研究所 一种利用微量临床穿刺样本进行染色质免疫共沉淀文库制备的方法及其应用
CN113661250A (zh) * 2019-02-11 2021-11-16 爱披萨佛公司 使用长读长测序的染色质作图测定和试剂盒
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
EP3924505B1 (en) 2019-02-12 2025-12-17 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
EP3924507A4 (en) * 2019-02-15 2022-11-30 Epicypher, Inc. QUANTITATIVE IMAGE OF CHROMATIN-ASSOCIATED PROTEINS
CN109880887A (zh) * 2019-03-20 2019-06-14 嘉兴菲沙基因信息有限公司 一种适用于植物ATAC-seq测序技术的文库构建方法
WO2020198664A1 (en) * 2019-03-28 2020-10-01 The Regents Of The University Of California Methods and systems for sequencing cell-free microbiome dna
KR102192455B1 (ko) * 2019-04-05 2020-12-17 한국과학기술원 개인의 전이효소-접근가능한 염색질 시퀀싱 정보를 이용한 암 진단 마커 및 이의 용도
WO2021011433A1 (en) * 2019-07-12 2021-01-21 New York Genome Center, Inc Methods and compositions for scalable pooled rna screens with single cell chromatin accessibility profiling
EP3999624B1 (en) 2019-07-16 2026-03-25 Meliolabs Inc. Methods and devices for single-cell based digital high resolution melt
US12571051B2 (en) * 2019-08-27 2026-03-10 Belgian Volition Srl Method of isolating circulating nucleosomes
KR20220080091A (ko) 2019-09-06 2022-06-14 체엠엠 - 포르슝스첸트룸 퓨어 몰레쿨라레 메디친 게엠베하 Rna 올리고뉴클레오티드를 시퀀싱하기 위한 방법
EP4081656A1 (en) * 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
CN115279917B (zh) * 2020-09-16 2025-10-10 深圳华大生命科学研究院 多维度解析细胞表观基因组学的方法
TW202242130A (zh) * 2020-12-29 2022-11-01 比利時商比利時意志有限公司 循環轉錄因子分析
EP4288534A1 (en) * 2021-02-05 2023-12-13 Ospedale San Raffaele S.r.l. Engineered transposase and uses thereof
CN112795563A (zh) * 2021-03-23 2021-05-14 上海欣百诺生物科技有限公司 生物素化的转座体在回收CUT&Tag或ATAC-seq产物中的用途及方法
CN113186174B (zh) * 2021-04-23 2022-04-15 江南大学 一种Tn5突变酶的制备和应用
CN113774052A (zh) * 2021-09-30 2021-12-10 首都医科大学附属北京安贞医院 mtDNA的提取和建库试剂
JP2025523739A (ja) * 2022-07-27 2025-07-25 イルミナ インコーポレイテッド タグメンテーションワークフロー
CN115948363B (zh) * 2022-08-26 2024-02-27 武汉影子基因科技有限公司 Tn5转座酶突变体及其制备方法和应用
CN116590283B (zh) * 2023-07-17 2023-11-07 北京微岩医疗器械有限公司 一种细胞结合增强剂和去除人源游离核酸的方法
CN117106857B (zh) * 2023-10-24 2024-02-09 北京大学 一种血浆游离染色质的检测方法、试剂盒及其应用
WO2025151541A1 (en) * 2024-01-08 2025-07-17 Massachusetts Eye And Ear Infirmary Extraction of genomic dna from fixed cells

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US5925545A (en) 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
US6406896B1 (en) 1999-08-02 2002-06-18 Wisconsin Alumni Research Foundation Transposase enzyme and method for use
US7067644B2 (en) 2000-12-05 2006-06-27 Wisconsin Alumni Research Foundation Double transposition methods for manipulating nucleic acids
US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US7316903B2 (en) 2003-03-28 2008-01-08 United States Of America As Represented By The Department Of Health And Human Services Detection of nucleic acid sequence variations using phase Mu transposase
WO2004093645A2 (en) 2003-04-17 2004-11-04 Wisconsin Alumni Research Foundation Tn5 transposase mutants and the use thereof
US7608434B2 (en) 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2012103545A1 (en) 2011-01-28 2012-08-02 Illumina, Inc. Oligonucleotide replacement for di-tagged and directional libraries
WO2013078470A2 (en) 2011-11-22 2013-05-30 MOTIF, Active Multiplex isolation of protein-associated nucleic acids
CN102703426A (zh) * 2012-05-21 2012-10-03 吴江汇杰生物科技有限公司 构建核酸库的方法、试剂及试剂盒
DK2999784T4 (en) * 2013-05-22 2023-02-20 Active Motif Inc Målrettet transposition til anvendelse i epigenetiske studier
US20160208323A1 (en) 2013-06-21 2016-07-21 The Broad Institute, Inc. Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing

Also Published As

Publication number Publication date
PL3334841T3 (pl) 2020-05-18
EP3674414A1 (en) 2020-07-01
SMT202000037T1 (it) 2020-03-13
US20180237951A1 (en) 2018-08-23
DK3334841T3 (da) 2020-01-20
EP3334841A1 (en) 2018-06-20
CA2995305A1 (en) 2017-02-16
CN108368540B (zh) 2022-10-11
PT3334841T (pt) 2020-01-30
US10934636B2 (en) 2021-03-02
CN108368540A (zh) 2018-08-03
EP3334841B1 (en) 2019-10-30
ES2779798T3 (es) 2020-08-19
RS59838B1 (sr) 2020-02-28
HRP20200062T1 (hr) 2020-04-03
WO2017025594A1 (en) 2017-02-16
LT3334841T (lt) 2020-02-10
JP2018532373A (ja) 2018-11-08
CY1122632T1 (el) 2021-03-12
SI3334841T1 (sl) 2020-03-31
HUE047175T2 (hu) 2020-04-28

Similar Documents

Publication Publication Date Title
JP6697070B2 (ja) 核酸の研究方法
US11885814B2 (en) High efficiency targeted in situ genome-wide profiling
US10914729B2 (en) Methods for detecting protein binding sequences and tagging nucleic acids
JP2023539980A (ja) シークエンシングによる標的タグメンテーションからのｒｎａ発現およびｄｎａに関する個々の細胞の並行解析
Fischer et al. p53 reveals principles of chromatin remodeling and enhancer activation
JP2025534400A (ja) 二本鎖ｄｎａデアミナーゼを用いたフットプリンティングによりゲノムワイドｄｎａ結合タンパク質結合部位を決定するための方法
EP4613870A1 (en) Methods for cut&tag
WO2025186424A1 (en) Methods for cut&tag
Kobayashi et al. Feed-forward loops by NR5A2 ensure robust gene activation during pre-implantation development
Marr Probing Genome-Wide Native Chromatin Structure via Histone Surface Accessibilities

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190809

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190809

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20190823

A975 Report on accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A971005

Effective date: 20190905

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191001

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20191227

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200228

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20200407

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20200423

R150 Certificate of patent or registration of utility model

Ref document number: 6697070

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250