JP2018521653A5 - - Google Patents
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- Publication number
- JP2018521653A5 JP2018521653A5 JP2018502076A JP2018502076A JP2018521653A5 JP 2018521653 A5 JP2018521653 A5 JP 2018521653A5 JP 2018502076 A JP2018502076 A JP 2018502076A JP 2018502076 A JP2018502076 A JP 2018502076A JP 2018521653 A5 JP2018521653 A5 JP 2018521653A5
- Authority
- JP
- Japan
- Prior art keywords
- amino acid
- epimerase
- tagatose
- acid residue
- hexuronic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 125000000539 amino acid group Chemical group 0.000 claims 8
- BJHIKXHVCXFQLS-UYFOZJQFSA-N Fructose Natural products OC[C@@H](O)[C@@H](O)[C@H](O)C(=O)CO BJHIKXHVCXFQLS-UYFOZJQFSA-N 0.000 claims 6
- CIWBSHSKHKDKBQ-SZSCBOSDSA-N 2-[(1S)-1,2-dihydroxyethyl]-3,4-dihydroxy-2H-furan-5-one Chemical compound OC[C@H](O)C1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-SZSCBOSDSA-N 0.000 claims 5
- LKDRXBCSQODPBY-OEXCPVAWSA-N D-tagatose Chemical compound OCC1(O)OC[C@@H](O)[C@H](O)[C@@H]1O LKDRXBCSQODPBY-OEXCPVAWSA-N 0.000 claims 4
- 238000004519 manufacturing process Methods 0.000 claims 4
- MTCFGRXMJLQNBG-UWTATZPHSA-N D-serine Chemical compound OC[C@@H](N)C(O)=O MTCFGRXMJLQNBG-UWTATZPHSA-N 0.000 claims 3
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 238000006345 epimerization reaction Methods 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 239000004475 Arginine Substances 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- QIVBCDIJIAJPQS-SECBINFHSA-N D-tryptophane Chemical compound C1=CC=C2C(C[C@@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-SECBINFHSA-N 0.000 claims 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 claims 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims 1
- 239000004473 Threonine Substances 0.000 claims 1
- 150000001413 amino acids Chemical group 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 239000008103 glucose Substances 0.000 claims 1
- 230000003301 hydrolyzing Effects 0.000 claims 1
- 239000002184 metal Substances 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 239000004474 valine Substances 0.000 claims 1
- LKDRXBCSQODPBY-DPYQTVNSSA-N β-D-tagatopyranose Chemical compound OC[C@@]1(O)OC[C@@H](O)[C@H](O)[C@@H]1O LKDRXBCSQODPBY-DPYQTVNSSA-N 0.000 claims 1
Claims (7)
- 配列番号１のアミノ酸配列で構成されたヘキスロン酸Ｃ４−エピメラーゼ（Ｈｅｘｕｒｏｎａｔｅ Ｃ４−ｅｐｉｍｅｒａｓｅ）のＮ−末端から１２５番セリン（Ｓ）のアミノ酸残基、２６７番バリン（Ｖ）のアミノ酸残基、２６８番セリン（Ｓ）のアミノ酸残基、３０６番トリプトファン（Ｗ）のアミノ酸残基と３８６番アルギニン（Ｒ）のアミノ酸残基が変異され、１８５番セリン（Ｓ）のアミノ酸残基、２７２番スレオニン（Ｔ）のアミノ酸残基、および４０３番チロシン（Ｙ）のアミノ酸残基が変異されていないヘキスロン酸Ｃ４−エピメラーゼ変異体。
- 請求項１に記載のヘキスロン酸Ｃ４−エピメラーゼ変異体をコードする核酸。
- 請求項２に記載の核酸を含む形質転換体。
- 請求項１に記載のヘキスロン酸Ｃ４−エピメラーゼ変異体を含む、タガトース生産用の組成物。
- 請求項１に記載のヘキスロン酸Ｃ４−エピメラーゼ変異体、請求項３に記載の形質転換体、または請求項４に記載のＤ−タガトース生産用の組成物とＤ−フルクトース（Ｄ−ｆｒｕｃｔｏｓｅ）を反応させて前記Ｄ−フルクトースをエピマー化（ｅｐｉｍｅｒｉｚａｔｉｏｎ）する工程を備える、Ｄ−タガトースの製造方法。
- 前記エピマー化する工程は、金属塩が存在する条件で行われることを特徴とする、請求項５に記載のＤ−タガトースの製造方法。
- 前記エピマー化する工程の前に、砂糖を酵素で加水分解してＤ−フルクトースを得る工程またはブドウ糖を酵素で異性化する工程をさらに備えることを特徴とする、請求項５に記載のＤ−タガトースの製造方法。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2015-0107436 | 2015-07-29 | ||
| KR20150107436 | 2015-07-29 | ||
| PCT/KR2016/008412 WO2017018863A1 (ko) | 2015-07-29 | 2016-07-29 | 전환 활성이 향상된 헥수론산 c4-에피머화 효소 변이체 및 이를 이용한 d-타가토스의 제조 방법 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2018521653A JP2018521653A (ja) | 2018-08-09 |
| JP2018521653A5 true JP2018521653A5 (ja) | 2019-09-05 |
Family
ID=57884824
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018502076A Pending JP2018521653A (ja) | 2015-07-29 | 2016-07-29 | 転換活性が高められたヘキスロン酸ｃ４−エピメラーゼ変異体及びこれを用いたｄ−タガトースの製造方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10196626B2 (ja) |
| EP (1) | EP3333260A4 (ja) |
| JP (1) | JP2018521653A (ja) |
| KR (2) | KR20170015250A (ja) |
| CN (1) | CN108138161B (ja) |
| HK (1) | HK1250049A1 (ja) |
| WO (1) | WO2017018863A1 (ja) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3006568B1 (en) * | 2013-06-05 | 2019-08-14 | Cj Cheiljedang Corporation | Production method for tagatose |
| US10196626B2 (en) * | 2015-07-29 | 2019-02-05 | Cj Cheiljedang Corporation | Hexuronate C4-epimerase mutant with improved conversion activity, and method for producing D-tagatose by using same |
| KR101946157B1 (ko) * | 2016-07-29 | 2019-02-11 | 씨제이제일제당 (주) | D-타가토스 전환 활성이 향상된 헥수론산 c4-에피머화 효소 변이체 및 이를 이용한 d-타가토스의 제조 방법 |
| KR101883351B1 (ko) * | 2016-12-20 | 2018-07-30 | (주)케비젠 | 과당에서 타가토스를 생산하는 능력이 개선된 헥수론산 c4-에피머화 효소 변이체 |
| KR20190001934A (ko) * | 2017-06-28 | 2019-01-07 | 주식회사 삼양사 | 기능성 감미료의 제조방법 |
| WO2019004702A2 (ko) * | 2017-06-28 | 2019-01-03 | 주식회사 삼양사 | 기능성 감미료의 제조방법 |
| US10963877B2 (en) | 2017-07-11 | 2021-03-30 | Mastercard International Incorporated | Systems and methods for use in authenticating users in connection with network transactions |
| KR101940785B1 (ko) * | 2017-11-21 | 2019-01-21 | (주)케비젠 | 써모토가 페트로필라 유래의 헥수론산 c4-에피머화 변이체 효소 및 이의 용도 |
| WO2020067781A1 (ko) * | 2018-09-28 | 2020-04-02 | 씨제이제일제당 (주) | 신규 과당-4-에피머화 효소 및 이를 이용한 타가토스의 제조 방법 |
| KR102409878B1 (ko) | 2018-09-28 | 2022-06-20 | 씨제이제일제당 주식회사 | 신규 과당-4-에피머화 효소 및 이를 이용한 타가토스의 제조 방법 |
| USD893758S1 (en) | 2018-12-10 | 2020-08-18 | EverBlock Systems, LLC | Wall connector |
| KR102131638B1 (ko) * | 2018-12-12 | 2020-07-08 | 대상 주식회사 | 과당으로부터 타가토스로의 전환 활성이 향상된 헥수론산 c4-에피머화 효소 변이체 |
| CN110396513B (zh) * | 2019-07-19 | 2022-01-11 | 天津科技大学 | 一种d-阿洛酮糖-3-差向异构酶的突变体及其应用 |
| KR102219195B1 (ko) * | 2019-08-14 | 2021-02-24 | 씨제이제일제당 주식회사 | 신규 과당-4-에피머화 효소 및 이를 이용한 타가토스의 제조 방법 |
| KR102399441B1 (ko) | 2020-01-20 | 2022-05-18 | 씨제이제일제당 주식회사 | 타가토스 생산용 조성물 및 이를 이용한 타가토스 제조 방법 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100483281B1 (ko) | 2000-12-19 | 2005-04-15 | 주식회사 오리온 | 신규한 내열성 갈락토스 이성화효소 및 그를 이용한타가토스 제조방법 |
| KR100964091B1 (ko) | 2008-01-28 | 2010-06-16 | 씨제이제일제당 (주) | 대두 올리고당을 이용한 타가토스의 제조 방법 |
| CN102421893B (zh) * | 2009-04-30 | 2014-10-29 | 花王株式会社 | 碱性蛋白酶变体 |
| CN102373230A (zh) * | 2010-08-27 | 2012-03-14 | 天津工业生物技术研究所 | 某种梭菌d-塔格糖3-差向异构酶的核苷酸序列及其应用 |
| KR20140111093A (ko) | 2013-03-06 | 2014-09-18 | 씨제이제일제당 (주) | 향상된 전환 활성을 가지는 l-아라비노스 이성화효소 변이체 및 이를 이용한 d-타가토스의 생산 방법 |
| EP3006568B1 (en) * | 2013-06-05 | 2019-08-14 | Cj Cheiljedang Corporation | Production method for tagatose |
| US10196626B2 (en) * | 2015-07-29 | 2019-02-05 | Cj Cheiljedang Corporation | Hexuronate C4-epimerase mutant with improved conversion activity, and method for producing D-tagatose by using same |
-
2016
- 2016-07-29 US US15/748,164 patent/US10196626B2/en active Active
- 2016-07-29 KR KR1020160097499A patent/KR20170015250A/ko not_active Application Discontinuation
- 2016-07-29 CN CN201680044426.4A patent/CN108138161B/zh active Active
- 2016-07-29 EP EP16830896.3A patent/EP3333260A4/en not_active Withdrawn
- 2016-07-29 WO PCT/KR2016/008412 patent/WO2017018863A1/ko active Application Filing
- 2016-07-29 JP JP2018502076A patent/JP2018521653A/ja active Pending
-
2017
- 2017-12-12 KR KR1020170170672A patent/KR20170141176A/ko not_active Application Discontinuation
-
2018
- 2018-07-20 HK HK18109468.4A patent/HK1250049A1/zh unknown
- 2018-12-21 US US16/231,239 patent/US10544409B2/en active Active
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