JP2018512431A5 - - Google Patents

Download PDF

Info

Publication number
JP2018512431A5
JP2018512431A5 JP2017553132A JP2017553132A JP2018512431A5 JP 2018512431 A5 JP2018512431 A5 JP 2018512431A5 JP 2017553132 A JP2017553132 A JP 2017553132A JP 2017553132 A JP2017553132 A JP 2017553132A JP 2018512431 A5 JP2018512431 A5 JP 2018512431A5
Authority
JP
Japan
Prior art keywords
less
units
unit
parent
antibody species
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2017553132A
Other languages
English (en)
Japanese (ja)
Other versions
JP6962819B2 (ja
JP2018512431A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2016/026620 external-priority patent/WO2016164708A1/en
Publication of JP2018512431A publication Critical patent/JP2018512431A/ja
Publication of JP2018512431A5 publication Critical patent/JP2018512431A5/ja
Application granted granted Critical
Publication of JP6962819B2 publication Critical patent/JP6962819B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2017553132A 2015-04-10 2016-04-08 親ホモ二量体抗体種からのヘテロ二量体多重特異性抗体の精製方法 Active JP6962819B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562146116P 2015-04-10 2015-04-10
US62/146,116 2015-04-10
US201562249180P 2015-10-30 2015-10-30
US62/249,180 2015-10-30
PCT/US2016/026620 WO2016164708A1 (en) 2015-04-10 2016-04-08 Methods for purifying heterodimeric multispecific antibodies from parental homodimeric antibody species

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020080220A Division JP7111767B2 (ja) 2015-04-10 2020-04-30 親ホモ二量体抗体種からのヘテロ二量体多重特異性抗体の精製方法

Publications (3)

Publication Number Publication Date
JP2018512431A JP2018512431A (ja) 2018-05-17
JP2018512431A5 true JP2018512431A5 (https=) 2019-05-09
JP6962819B2 JP6962819B2 (ja) 2021-11-05

Family

ID=57072068

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2017553132A Active JP6962819B2 (ja) 2015-04-10 2016-04-08 親ホモ二量体抗体種からのヘテロ二量体多重特異性抗体の精製方法
JP2020080220A Active JP7111767B2 (ja) 2015-04-10 2020-04-30 親ホモ二量体抗体種からのヘテロ二量体多重特異性抗体の精製方法
JP2021085111A Pending JP2021119207A (ja) 2015-04-10 2021-05-20 親ホモ二量体抗体種からのヘテロ二量体多重特異性抗体の精製方法

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2020080220A Active JP7111767B2 (ja) 2015-04-10 2020-04-30 親ホモ二量体抗体種からのヘテロ二量体多重特異性抗体の精製方法
JP2021085111A Pending JP2021119207A (ja) 2015-04-10 2021-05-20 親ホモ二量体抗体種からのヘテロ二量体多重特異性抗体の精製方法

Country Status (6)

Country Link
US (2) US10787500B2 (https=)
EP (1) EP3280444B1 (https=)
JP (3) JP6962819B2 (https=)
CN (1) CN108472360B (https=)
AU (2) AU2016245887B2 (https=)
WO (1) WO2016164708A1 (https=)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2892925T3 (es) 2006-03-31 2022-02-07 Chugai Pharmaceutical Co Ltd Métodos para controlar la farmacocinética en sangre de anticuerpos
CN101874042B9 (zh) 2007-09-26 2019-01-01 中外制药株式会社 利用cdr的氨基酸取代来改变抗体等电点的方法
EP3126384B1 (en) * 2014-04-01 2020-12-02 Adimab, LLC Multispecific antibody analogs comprising a common light chain, and methods of their preparation and use
TWI700300B (zh) 2014-09-26 2020-08-01 日商中外製藥股份有限公司 中和具有第ｖｉｉｉ凝血因子（ｆｖｉｉｉ）機能替代活性的物質之抗體
JP7082484B2 (ja) 2015-04-01 2022-06-08 中外製薬株式会社 ポリペプチド異種多量体の製造方法
AU2016245887B2 (en) 2015-04-10 2021-09-23 Adimab, Llc. Methods for purifying heterodimeric multispecific antibodies from parental homodimeric antibody species
US20200270363A1 (en) 2015-12-25 2020-08-27 Chugai Seiyaku Kabushiki Kaisha Antibody having enhanced activity, and method for modifying same
CN108368166B (zh) 2015-12-28 2023-03-28 中外制药株式会社 提高含fc区多肽纯化效率的方法
MA44780A (fr) 2016-04-28 2019-03-06 Chugai Pharmaceutical Co Ltd Préparation contenant un anticorps
KR102591955B1 (ko) 2016-07-29 2023-10-19 추가이 세이야쿠 가부시키가이샤 증강된 fviii 보인자 기능 대체 활성을 갖는 이중특이성 항체
RU2766233C2 (ru) 2016-09-06 2022-02-10 Чугаи Сейяку Кабусики Кайся Способы применения биспецифического антитела, которое распознает фактор свертывания ix и/или активированный фактор свертывания ix и фактор свертывания x и/или активированный фактор свертывания x
CN110461358A (zh) 2017-03-31 2019-11-15 公立大学法人奈良县立医科大学 可用于预防和/或治疗凝血因子ⅸ异常、包含代替凝血因子ⅷ的功能的多特异性抗原结合分子的药物组合物
AR113142A1 (es) 2017-09-29 2020-01-29 Chugai Pharmaceutical Co Ltd Moléculas de unión al antígeno multiespecíficas que tienen actividad de sustitución de la función de cofactor del factor viii de coagulación de sangre (fviii), y formulaciones farmacéuticas que contienen dicha molécula como ingrediente activo
AU2018361430B2 (en) * 2017-11-01 2025-08-14 Chugai Seiyaku Kabushiki Kaisha Antibody variant and isoform with lowered biological activity
MX2021001915A (es) * 2018-08-17 2021-05-31 Regeneron Pharma Metodo y sistema de cromatografia para determinar la cantidad y pureza de una proteina multimerica.
CN112839959A (zh) * 2018-09-21 2021-05-25 特尼奥生物股份有限公司 用于纯化异源二聚多特异性抗体的方法
US11022585B2 (en) 2019-06-09 2021-06-01 Dionex Corporation Methods and systems for optimizing buffer conditions with liquid chromatography
CN114539416B (zh) * 2020-11-26 2024-11-08 盛禾(中国)生物制药有限公司 一种双特异性抗体的层析纯化工艺

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US459004A (en) 1891-09-08 Box for steaming cops
US8188A (en) 1851-07-01 Htkstaht
US231A (en) 1837-06-10 Stove for heating irons for the use of tailors
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US6291159B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
US6291158B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertoire
ES2078518T3 (es) 1990-04-05 1995-12-16 Roberto Crea Mutagenesis por desplazamiento completo.
ES2097363T3 (es) 1991-10-11 1997-04-01 Promega Corp Transcripcion y traduccion acopladas en un extracto exento de celulas eucariotas.
US5492817A (en) 1993-11-09 1996-02-20 Promega Corporation Coupled transcription and translation in eukaryotic cell-free extract
US5665563A (en) 1991-10-11 1997-09-09 Promega Corporation Coupled transcription and translation in eukaryotic cell-free extract
US5866344A (en) 1991-11-15 1999-02-02 Board Of Regents, The University Of Texas System Antibody selection methods using cell surface expressed libraries
US20030036092A1 (en) 1991-11-15 2003-02-20 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
US5667988A (en) 1992-01-27 1997-09-16 The Scripps Research Institute Methods for producing antibody libraries using universal or randomized immunoglobulin light chains
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
US6696248B1 (en) 1995-08-18 2004-02-24 Morphosys Ag Protein/(poly)peptide libraries
AU725609C (en) 1995-08-18 2002-01-03 Morphosys Ag Protein/(poly)peptide libraries
US6165718A (en) 1996-01-10 2000-12-26 Novo Nordisk A/S Novo Alle Method for in vivo production of a mutant library in cells
US6261804B1 (en) 1997-01-21 2001-07-17 The General Hospital Corporation Selection of proteins using RNA-protein fusions
DK1712623T3 (da) 1997-01-21 2012-02-06 Gen Hospital Corp Udvælgelse af proteiner ved anvendelse af RNA-proteinfusioner
JP4093507B2 (ja) * 1997-03-10 2008-06-04 旭化成ファーマ株式会社 緩衝液組成
US6159688A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods of producing polynucleotide variants
DE69834032T2 (de) 1997-04-23 2006-12-07 Universität Zürich Verfahren zur erkennung von nucleinsäuremolekülen codierend für (poly)peptide, welche mit zielmolekülen interagieren
US7951917B1 (en) 1997-05-02 2011-05-31 Genentech, Inc. Method for making multispecific antibodies having heteromultimeric and common components
ES2430857T3 (es) 2000-12-18 2013-11-22 Dyax Corp. Bibliotecas focalizadas de paquetes genéticos
US20060235208A1 (en) 2002-09-27 2006-10-19 Xencor, Inc. Fc variants with optimized properties
US20060134105A1 (en) 2004-10-21 2006-06-22 Xencor, Inc. IgG immunoglobulin variants with optimized effector function
JP2008512352A (ja) 2004-07-17 2008-04-24 イムクローン システムズ インコーポレイティド 新規な四価の二重特異性抗体
CA2603264C (en) * 2005-04-08 2017-03-21 Chugai Seiyaku Kabushiki Kaisha Antibody substituting for function of blood coagulation factor viii
CN101460622A (zh) * 2006-03-31 2009-06-17 中外制药株式会社 用于纯化双特异性抗体的抗体修饰方法
US9670269B2 (en) 2006-03-31 2017-06-06 Chugai Seiyaku Kabushiki Kaisha Methods of modifying antibodies for purification of bispecific antibodies
ATE554397T1 (de) 2006-12-21 2012-05-15 Sekisui Medical Co Ltd Verfahren zur stabilisierung von alpha-thrombin in einer thrombinhaltigen lösung
CA2693053C (en) 2007-05-30 2021-01-05 Xencor, Inc. Methods and compositions for inhibiting cd32b expressing cells
CA2697922A1 (en) 2007-08-28 2009-03-12 Biogen Idec Ma Inc. Compositions that bind multiple epitopes of igf-1r
US8691730B2 (en) 2007-09-14 2014-04-08 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
CN101874042B9 (zh) * 2007-09-26 2019-01-01 中外制药株式会社 利用cdr的氨基酸取代来改变抗体等电点的方法
ES2563027T3 (es) 2008-01-07 2016-03-10 Amgen Inc. Método para fabricación de moléculas heterodímeras Fc de anticuerpos utilizando efectos de conducción electrostática
US8791882B2 (en) 2008-03-06 2014-07-29 Sharp Kabushiki Kaisha Display device of active matrix type
PE20100092A1 (es) 2008-06-03 2010-03-12 Abbott Lab Inmunoglobulina con dominio variable dual y usos de la misma
SG178078A1 (en) * 2009-07-24 2012-03-29 Hoffmann La Roche Optimizing the production of antibodies
US9493578B2 (en) 2009-09-02 2016-11-15 Xencor, Inc. Compositions and methods for simultaneous bivalent and monovalent co-engagement of antigens
EP2507381A4 (en) * 2009-12-04 2016-07-20 Hoffmann La Roche MULTISPECIFIC ANTIBODIES, ANTIBODY ANALOGS, COMPOSITIONS AND METHODS
CN102946906B (zh) * 2010-04-23 2015-07-15 弗·哈夫曼-拉罗切有限公司 生产异源多聚体蛋白质
DK2593594T3 (en) 2010-07-16 2017-12-11 Adimab Llc ANTIBODY LIBRARIES
WO2012015912A1 (en) 2010-07-30 2012-02-02 Medimmune, Llc Method for purifying active polypeptides or immunocojugates
US10344050B2 (en) * 2011-10-27 2019-07-09 Genmab A/S Production of heterodimeric proteins
PT2825559T (pt) 2012-03-13 2019-06-07 Novimmune Sa Anticorpos biespecíficos prontamente isolados com formato de imunoglobulina nativo
CN105051528A (zh) * 2012-11-15 2015-11-11 弗·哈夫曼-拉罗切有限公司 离子强度介导的pH梯度离子交换色谱
AU2016245887B2 (en) 2015-04-10 2021-09-23 Adimab, Llc. Methods for purifying heterodimeric multispecific antibodies from parental homodimeric antibody species

Similar Documents

Publication Publication Date Title
JP2018512431A5 (https=)
Gomis‐Fons et al. Model‐based design and control of a small‐scale integrated continuous end‐to‐end mAb platform
Westermeier et al. Proteomics in practice: a guide to successful experimental design
Stein et al. Cation exchange chromatography in antibody purification: pH screening for optimised binding and HCP removal
Nfor et al. Multi‐dimensional fractionation and characterization of crude protein mixtures: toward establishment of a database of protein purification process development parameters
Buyel et al. Generic chromatography‐based purification strategies accelerate the development of downstream processes for biopharmaceutical proteins produced in plants
RU2017100005A (ru) Способ очистки белка слияния
ES3053015T3 (en) Chromatography
JP2015537212A5 (https=)
Rathore et al. Scale-up and optimization in preparative chromatography
JP2018516229A5 (https=)
Heiss et al. Identification and deletion of the major secreted protein of Pichia pastoris
ES2988082T3 (es) Elución de biomoléculas a partir de resinas multimodales utilizando MES y MOPS como modificadores de fase móvil
IL297116A (en) A method for purifying single-stranded RNA
Muthukumar et al. Economic benefits of membrane chromatography versus packed bed column purification of therapeutic proteins expressed in microbial and mammalian hosts
RU2016104542A (ru) Осуществление оптимизации входных условий ионообменной хроматографии
Zhou et al. Large‐scale identification of human biliary proteins from a cholesterol stone patient using a proteomic approach
Connell‐Crowley et al. Cation exchange chromatography provides effective retrovirus clearance for antibody purification processes
JP2016529489A5 (https=)
CN104136121A (zh) 减少离子交换色谱中的pH漂移
Chen et al. Partition coefficient screening–An effective approach for finding the best conditions for byproduct removal as demonstrated by a bispecific antibody purification case
Morrow Jr Advances in antibody manufacturing using mammalian cells
EP3138913A1 (en) Method and kit for selective isolation of nucleic acid
Hedrich et al. Mixed‐mode membrane chromatography: mechanistic‐modeling‐based optimization and scale‐up of antibody charge variant separation using a novel membrane adsorber
CN118414350A (zh) 纯化具有igg fc结构域的融合蛋白的方法