JP2018075021A5 - - Google Patents
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- Publication number
- JP2018075021A5 JP2018075021A5 JP2017236383A JP2017236383A JP2018075021A5 JP 2018075021 A5 JP2018075021 A5 JP 2018075021A5 JP 2017236383 A JP2017236383 A JP 2017236383A JP 2017236383 A JP2017236383 A JP 2017236383A JP 2018075021 A5 JP2018075021 A5 JP 2018075021A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- nanopore
- translocase
- electrical signal
- translocated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 claims 17
- 102000004169 proteins and genes Human genes 0.000 claims 17
- 102000017033 Porins Human genes 0.000 claims 4
- 108010013381 Porins Proteins 0.000 claims 4
- 230000005945 translocation Effects 0.000 claims 4
- 239000012528 membrane Substances 0.000 claims 3
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 239000012530 fluid Substances 0.000 claims 2
- 239000007787 solid Substances 0.000 claims 2
- 101710092462 Alpha-hemolysin Proteins 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 101150096566 clpX gene Proteins 0.000 claims 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261599754P | 2012-02-16 | 2012-02-16 | |
| US61/599,754 | 2012-02-16 | ||
| US201261713163P | 2012-10-12 | 2012-10-12 | |
| US61/713,163 | 2012-10-12 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014557827A Division JP6312607B2 (ja) | 2012-02-16 | 2013-02-15 | 酵素仲介タンパク質トランスロケーションのためのナノポアセンサー |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018075021A JP2018075021A (ja) | 2018-05-17 |
| JP2018075021A5 true JP2018075021A5 (enExample) | 2018-06-28 |
| JP6594944B2 JP6594944B2 (ja) | 2019-10-23 |
Family
ID=48984893
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014557827A Active JP6312607B2 (ja) | 2012-02-16 | 2013-02-15 | 酵素仲介タンパク質トランスロケーションのためのナノポアセンサー |
| JP2017236383A Active JP6594944B2 (ja) | 2012-02-16 | 2017-12-08 | 酵素仲介タンパク質トランスロケーションのためのナノポアセンサー |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014557827A Active JP6312607B2 (ja) | 2012-02-16 | 2013-02-15 | 酵素仲介タンパク質トランスロケーションのためのナノポアセンサー |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US11339365B2 (enExample) |
| EP (1) | EP2814939B1 (enExample) |
| JP (2) | JP6312607B2 (enExample) |
| KR (1) | KR102066758B1 (enExample) |
| CN (1) | CN104619854B (enExample) |
| AU (1) | AU2013221356B2 (enExample) |
| BR (1) | BR112014020255B1 (enExample) |
| CA (1) | CA2864824C (enExample) |
| DK (1) | DK2814939T3 (enExample) |
| WO (1) | WO2013123379A2 (enExample) |
Families Citing this family (63)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| EP2694671B1 (en) | 2011-04-04 | 2021-01-06 | President and Fellows of Harvard College | Nanopore sensor comprising a device for measuring the local electrical potential |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| EP3736339B1 (en) | 2012-02-16 | 2022-07-27 | Oxford Nanopore Technologies plc | Analysis of measurements of a polymer |
| US9274430B2 (en) | 2012-10-10 | 2016-03-01 | Arizona Board Of Regents On Behalf Of Arizona State University | Systems and devices for molecule sensing and method of manufacturing thereof |
| GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
| EP3462167A1 (en) | 2013-03-05 | 2019-04-03 | Arizona Board of Regents on behalf of Arizona State University | Translocation of a polymer through a nanopore |
| GB201316849D0 (en) * | 2013-09-23 | 2013-11-06 | Isis Innovation | Method |
| WO2015127387A1 (en) * | 2014-02-21 | 2015-08-27 | Northeastern University | Fluorescence-based analysis of biopolymers using nanopores |
| US10336713B2 (en) | 2014-02-27 | 2019-07-02 | Arizona Board Of Regents, Acting For And On Behalf Of, Arizona State University | Triazole-based reader molecules and methods for synthesizing and use thereof |
| CA3163156C (en) * | 2014-07-31 | 2025-07-08 | Illumina Inc | HYBRID NANOPORE SENSORS |
| CN115851894B (zh) | 2014-10-16 | 2025-06-27 | 牛津楠路珀尔科技股份有限公司 | 聚合物的分析 |
| US20160194698A1 (en) * | 2014-12-16 | 2016-07-07 | Arizona Board Of Regents On Behalf Of Arizona State University | Systems, apparatuses and methods for reading polymer sequence |
| US10794895B2 (en) | 2015-02-05 | 2020-10-06 | President And Fellows Of Harvard College | Nanopore sensor including fluidic passage |
| GB201508669D0 (en) | 2015-05-20 | 2015-07-01 | Oxford Nanopore Tech Ltd | Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown |
| US10126262B2 (en) * | 2015-09-24 | 2018-11-13 | Genia Technologies, Inc. | Differential output of analog memories storing nanopore measurement samples |
| GB201611770D0 (en) | 2016-07-06 | 2016-08-17 | Oxford Nanopore Tech | Microfluidic device |
| CN106443008A (zh) * | 2016-08-31 | 2017-02-22 | 中国科学院重庆绿色智能技术研究院 | 一种基于固态纳米孔的hiv‑1蛋白酶检测方法 |
| GB201619930D0 (en) * | 2016-11-24 | 2017-01-11 | Oxford Nanopore Tech | Apparatus and methods for controlling insertion of a membrane channel into a membrane |
| KR102593484B1 (ko) | 2017-06-29 | 2023-10-24 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 나노포어를 통한 폴리머의 결정적 스텝핑 |
| EP3655423B1 (en) * | 2017-07-17 | 2023-12-20 | President and Fellows of Harvard College | Nanopore-matched protein shuttle for molecular characterization |
| WO2019081442A1 (en) | 2017-10-23 | 2019-05-02 | F. Hoffmann-La Roche Ag | REMOVAL AND REINTEGRATION OF PROTEIN NANOPORES IN A MEMBRANE USING AN OSMOTIC IMBALANCE |
| GB2568895B (en) | 2017-11-29 | 2021-10-27 | Oxford Nanopore Tech Ltd | Microfluidic device |
| CN108226249B (zh) * | 2018-01-09 | 2021-02-26 | 深圳市梅丽纳米孔科技有限公司 | 一次性纳米孔生物传感器及其制作方法 |
| WO2019226689A1 (en) | 2018-05-22 | 2019-11-28 | Axbio Inc. | Methods, systems, and compositions for nucleic acid sequencing |
| IL280880B2 (en) | 2018-08-27 | 2025-04-01 | Regeneron Pharma | Using Raman Spectroscopy in Downstream Purification |
| BR112021004675A2 (pt) | 2018-09-11 | 2021-06-01 | Rijksuniversiteit Groningen | nanoporos biológicos com diâmetros de poros ajustáveis e usos dos mesmos como ferramentas analíticas |
| JP7474245B2 (ja) * | 2018-09-19 | 2024-04-24 | コナゲン インコーポレイテッド | コリネバクテリウム宿主細胞における操作した分解タグ変異体による制御可能なタンパク質分解 |
| WO2020073008A1 (en) * | 2018-10-05 | 2020-04-09 | University Of Washington | Reporter constructs for nanopore-based detection of biological activity |
| CN113260449B (zh) * | 2018-12-11 | 2023-09-29 | 豪夫迈·罗氏有限公司 | 用于膜中自限性蛋白质孔插入的系统和方法 |
| KR20210138594A (ko) | 2019-03-12 | 2021-11-19 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 나노포어 감지 디바이스 및 이를 작동하는 방법 및 형성하는 방법 |
| NL2023062B1 (en) * | 2019-05-03 | 2020-11-30 | Univ Delft Tech | Protein current trace signal acquisition using a nanopore |
| GB201907243D0 (en) * | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Sensing interactions between molecular entities and nanapores |
| CN112147185B (zh) * | 2019-06-29 | 2022-07-01 | 清华大学 | 一种控制多肽穿过纳米孔速度的方法及其应用 |
| CN110554079B (zh) * | 2019-09-26 | 2024-10-22 | 中国科学院重庆绿色智能技术研究院 | 一种基于纳米通道的抗体单分子检测系统及方法 |
| GB201915480D0 (en) | 2019-10-25 | 2019-12-11 | Oxford Nanopore Tech Ltd | Improved nanopore sensing device, components and method of manufacture |
| WO2021097349A1 (en) * | 2019-11-15 | 2021-05-20 | Machine Bio Inc. | Methods and systems for generating biological molecules |
| BR112022009402A2 (pt) * | 2019-11-19 | 2022-08-09 | Univ Groningen | Nanoporos artificiais e usos e métodos relacionados com os mesmos |
| CN118011004A (zh) * | 2019-12-02 | 2024-05-10 | 牛津纳米孔科技公开有限公司 | 使用纳米孔表征靶多肽的方法 |
| NL2024579B1 (en) | 2019-12-24 | 2021-09-06 | Univ Delft Tech | Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes |
| US20230194499A1 (en) * | 2020-04-14 | 2023-06-22 | Geneus Technologies Chengdu ) Co., Ltd. | Nanopore preparation and detection method and detection apparatus thereof |
| GB202016874D0 (en) | 2020-10-23 | 2020-12-09 | Oxford Nanopore Tech Ltd | Nanopore support structure and manufacture thereof |
| WO2022013551A1 (en) | 2020-07-17 | 2022-01-20 | Oxford Nanopore Technologies Limited | Nanopore sensing device |
| WO2022020461A1 (en) | 2020-07-22 | 2022-01-27 | Oxford Nanopore Technologies Inc. | Solid state nanopore formation |
| US11994508B2 (en) * | 2020-12-23 | 2024-05-28 | Northeastern University | Method and system for linearization and translocation of single protein molecules through nanopores |
| US11994484B2 (en) * | 2021-03-25 | 2024-05-28 | The Regents Of The University Of California | Apparatus and method for single cell discrimination |
| GB202112235D0 (en) | 2021-08-26 | 2021-10-13 | Oxford Nanopore Tech Ltd | Nanopore |
| WO2023055752A2 (en) | 2021-09-28 | 2023-04-06 | Axbio Inc. | Methods for processing a nucleic acid sample and compositions thereof |
| GB202118908D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| CN120092180A (zh) * | 2022-08-02 | 2025-06-03 | 格里菲克生物技术公司 | 通过可聚合分子偶联进行蛋白质测序 |
| IL320502A (en) | 2022-10-28 | 2025-06-01 | Univ Groningen | Nanopore-based protein efflux |
| GB202216162D0 (en) | 2022-10-31 | 2022-12-14 | Oxford Nanopore Tech Plc | Method |
| GB202216905D0 (en) | 2022-11-11 | 2022-12-28 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| WO2024138570A1 (zh) * | 2022-12-29 | 2024-07-04 | 深圳华大生命科学研究院 | 生物纳米孔传感器、其制备方法及应用 |
| WO2024165853A1 (en) | 2023-02-07 | 2024-08-15 | Oxford University Innovation Limited | Method of characterising a peptide, polypeptide or protein using a nanopore |
| WO2024222911A1 (zh) * | 2023-04-28 | 2024-10-31 | 清华大学 | 一种被修饰的多肽、多肽复合物、多肽混合物及控制多肽移位速度或表征多肽的系统和方法 |
| WO2024249122A1 (en) * | 2023-06-01 | 2024-12-05 | University Of Washington | Method and kit for rereading single protein molecules using a nanopore and an unfoldase |
| GB202313202D0 (en) * | 2023-08-30 | 2023-10-11 | Portal Biotech Ltd | Systems and methods for nanopore-based analyte detection |
| CN119667132A (zh) * | 2023-09-20 | 2025-03-21 | 西安电子科技大学 | 一种半合成纳米孔及其制备方法和蛋白质测序方法 |
| GB202317028D0 (en) | 2023-11-06 | 2023-12-20 | Oxford Nanopore Tech Ltd | Method |
| WO2025120010A1 (en) * | 2023-12-04 | 2025-06-12 | Adolphe Merkle Institute, University Of Fribourg | Ply nanopores |
| WO2025120150A1 (en) | 2023-12-08 | 2025-06-12 | Oxford Nanopore Technologies Plc | Methods and apparatus for forming apertures, methods and apparatus for unblocking apertures, methods of sensing molecular entities in apertures, and measurement systems |
| CN119470870B (zh) * | 2024-11-19 | 2025-10-17 | 广东工业大学 | 一种基于组合式纳米孔结构的蛋白质检测方法 |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| SE513280C2 (sv) | 1998-11-05 | 2000-08-14 | Forbo Int Sa | Beläggningsmaterial för golv och förfarande för dess framställning |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| US6464842B1 (en) | 1999-06-22 | 2002-10-15 | President And Fellows Of Harvard College | Control of solid state dimensional features |
| JP2003502166A (ja) | 1999-06-22 | 2003-01-21 | プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ | 固体状態の次元的特徴の制御 |
| DE60025739T2 (de) | 1999-09-07 | 2006-08-31 | The Regents Of The University Of California, Oakland | Verfahren um die anwesenheit von doppelsträngiger dns in einer probe nachzuweisen |
| AU2002239284A1 (en) | 2000-11-27 | 2002-06-03 | The Regents Of The University Of California | Methods and devices for characterizing duplex nucleic acid molecules |
| US20030104428A1 (en) | 2001-06-21 | 2003-06-05 | President And Fellows Of Harvard College | Method for characterization of nucleic acid molecules |
| US8278055B2 (en) | 2002-05-01 | 2012-10-02 | Intel Corporation | Methods and device for analyte characterization |
| US6870361B2 (en) | 2002-12-21 | 2005-03-22 | Agilent Technologies, Inc. | System with nano-scale conductor and nano-opening |
| US7846738B2 (en) * | 2003-08-15 | 2010-12-07 | President And Fellows Of Harvard College | Study of polymer molecules and conformations with a nanopore |
| WO2006028508A2 (en) | 2004-03-23 | 2006-03-16 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| WO2005124888A1 (en) | 2004-06-08 | 2005-12-29 | President And Fellows Of Harvard College | Suspended carbon nanotube field effect transistor |
| DE102005013608A1 (de) * | 2005-03-24 | 2006-09-28 | Westfälische Wilhelms-Universität Münster | Vorrichtung und Verfahren für die zellfreie analytische und präparative Proteinsynthese |
| AU2008236694B2 (en) | 2007-04-04 | 2014-01-23 | The Regents Of The University Of California | Compositions, devices, systems, and methods for using a nanopore |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| US8034910B2 (en) | 2008-05-06 | 2011-10-11 | Academia Sinica, Taiwan | SUMO fusion protein expression system for producing native proteins |
| GB0820927D0 (en) * | 2008-11-14 | 2008-12-24 | Isis Innovation | Method |
| WO2010082860A1 (en) | 2009-01-19 | 2010-07-22 | Instituto De Biologia Experimental E Tecnologia (Ibet) | Method and device for nanopore based single-molecule protein/ protein interaction detection |
| GB0905140D0 (en) * | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| JP5781502B2 (ja) | 2009-04-20 | 2015-09-24 | オックスフォード ナノポール テクノロジーズ リミテッド | 脂質二重層センサアレイ |
| US10385367B2 (en) * | 2009-06-01 | 2019-08-20 | Ginkgo Bioworks, Inc. | Methods and molecules for yield improvement involving metabolic engineering |
| BR112012013074B1 (pt) | 2009-12-01 | 2018-09-18 | Oxford Nanopore Technologies Limited | instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica |
| US9605307B2 (en) | 2010-02-08 | 2017-03-28 | Genia Technologies, Inc. | Systems and methods for forming a nanopore in a lipid bilayer |
| CA2790672C (en) * | 2010-02-23 | 2018-05-29 | University Of Washington | Artificial mycolic acid membranes |
| EP2588613A4 (en) * | 2010-06-30 | 2013-12-04 | Univ Syracuse | GENERALIZED PROTEIN PORES |
-
2013
- 2013-02-15 JP JP2014557827A patent/JP6312607B2/ja active Active
- 2013-02-15 KR KR1020147025764A patent/KR102066758B1/ko active Active
- 2013-02-15 CA CA2864824A patent/CA2864824C/en active Active
- 2013-02-15 EP EP13749423.3A patent/EP2814939B1/en active Active
- 2013-02-15 US US14/378,448 patent/US11339365B2/en active Active
- 2013-02-15 WO PCT/US2013/026414 patent/WO2013123379A2/en not_active Ceased
- 2013-02-15 AU AU2013221356A patent/AU2013221356B2/en active Active
- 2013-02-15 BR BR112014020255-9A patent/BR112014020255B1/pt active IP Right Grant
- 2013-02-15 DK DK13749423.3T patent/DK2814939T3/en active
- 2013-02-15 CN CN201380019821.3A patent/CN104619854B/zh active Active
-
2017
- 2017-12-08 JP JP2017236383A patent/JP6594944B2/ja active Active
-
2022
- 2022-05-16 US US17/745,399 patent/US20220396758A1/en active Pending
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