CN104619854B - 用于酶介导的蛋白质移位的纳米孔传感器 - Google Patents

用于酶介导的蛋白质移位的纳米孔传感器 Download PDF

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CN104619854B
CN104619854B CN201380019821.3A CN201380019821A CN104619854B CN 104619854 B CN104619854 B CN 104619854B CN 201380019821 A CN201380019821 A CN 201380019821A CN 104619854 B CN104619854 B CN 104619854B
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protein
pore
nano
translocase
shifted
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CN104619854A (zh
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J.M.尼瓦拉
D.B.马克斯
M.A.阿克森
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University of California San Diego UCSD
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Organic Chemistry (AREA)
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  • Biotechnology (AREA)
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  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Sustainable Development (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201380019821.3A 2012-02-16 2013-02-15 用于酶介导的蛋白质移位的纳米孔传感器 Active CN104619854B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261599754P 2012-02-16 2012-02-16
US61/599,754 2012-02-16
US201261713163P 2012-10-12 2012-10-12
US61/713,163 2012-10-12
PCT/US2013/026414 WO2013123379A2 (en) 2012-02-16 2013-02-15 Nanopore sensor for enzyme-mediated protein translocation

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CN104619854A CN104619854A (zh) 2015-05-13
CN104619854B true CN104619854B (zh) 2018-07-17

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US (2) US11339365B2 (enExample)
EP (1) EP2814939B1 (enExample)
JP (2) JP6312607B2 (enExample)
KR (1) KR102066758B1 (enExample)
CN (1) CN104619854B (enExample)
AU (1) AU2013221356B2 (enExample)
BR (1) BR112014020255B1 (enExample)
CA (1) CA2864824C (enExample)
DK (1) DK2814939T3 (enExample)
WO (1) WO2013123379A2 (enExample)

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AU2013221356A1 (en) 2014-08-28
AU2013221356B2 (en) 2018-10-18
CN104619854A (zh) 2015-05-13
US20220396758A1 (en) 2022-12-15
US11339365B2 (en) 2022-05-24
WO2013123379A3 (en) 2015-03-12
CA2864824C (en) 2020-06-16
EP2814939A4 (en) 2016-02-24
CA2864824A1 (en) 2013-08-22
EP2814939B1 (en) 2018-04-18
BR112014020255B1 (pt) 2022-01-25
KR20140131539A (ko) 2014-11-13
WO2013123379A2 (en) 2013-08-22
BR112014020255A2 (enExample) 2017-06-20
JP6594944B2 (ja) 2019-10-23
KR102066758B1 (ko) 2020-02-11
JP2015517799A (ja) 2015-06-25
JP2018075021A (ja) 2018-05-17
DK2814939T3 (en) 2018-07-30
US20160032236A1 (en) 2016-02-04
BR112014020255A8 (pt) 2017-07-11
JP6312607B2 (ja) 2018-04-18
EP2814939A2 (en) 2014-12-24

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